7 Questions
What is Agrobacterium-mediated transformation?
A method for plant genetic modification that involves the use of bacteria
What is the binary vector system?
A two-plasmid system that is effective for transforming plant cells
Which of the following can be used as explants for transformation?
Leaf pieces, cotyledons, stem segments, callus suspension cultures, and germinating seeds
Why are viruses not routinely used as vectors for gene transfer in plants?
Because they are not effective in transferring genes to plants
What is biolistics?
A method for plant genetic modification that involves the use of high-velocity particles
What is electroporation?
A method for plant genetic modification that involves the use of electricity
What are the genetic elements that constitute the T-DNA transfer machinery?
T-DNA, Agrobacterium chromosomal virulence genes, and Ti plasmid virulence genes
Study Notes
- Agrobacterium-mediated transformation is a common method for plant genetic modification.
- Tumorigenic genes are removed from the bacterium during transformation.
- The binary vector system is a two-plasmid system that is effective for transforming plant cells.
- Leaf pieces, cotyledons, stem segments, callus suspension cultures, and germinating seeds can be used as explants for transformation.
- Viruses have been investigated as vectors for gene transfer in plants, but are not routinely used.
- Biolistics is the most effective and widely used method for generating commercial transgenic crops.
- Physico-chemical uptake of DNA by protoplasts and cells can be used to produce transgenic plants.
- Electroporation can induce pores in the protoplast membrane to allow for DNA uptake.
- T-DNA, Agrobacterium chromosomal virulence genes, and Ti plasmid virulence genes are the genetic elements that constitute the T-DNA transfer machinery.
- An efficient tissue culture system is required for the regeneration of transformant cells.
Are you interested in plant genetic modification? Test your knowledge with this quiz on Agrobacterium-mediated transformation, binary vector systems, explants, and other methods for generating transgenic plants. Learn about the genetic elements involved in T-DNA transfer machinery and the tissue culture systems required for successful transformation. Sharpen your understanding of the latest techniques and technologies in plant biotechnology.
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