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Questions and Answers
What is Agrobacterium-mediated transformation?
What is Agrobacterium-mediated transformation?
- A common method for animal genetic modification
- A method for plant genetic modification that involves the use of chemicals
- A method for plant genetic modification that involves the use of bacteria (correct)
- A method for plant genetic modification that involves the use of viruses
What is the binary vector system?
What is the binary vector system?
- A three-plasmid system that is effective for transforming plant cells
- A two-plasmid system that is effective for transforming animal cells
- A one-plasmid system that is effective for transforming plant cells
- A two-plasmid system that is effective for transforming plant cells (correct)
Which of the following can be used as explants for transformation?
Which of the following can be used as explants for transformation?
- Only stem segments and callus suspension cultures
- Only leaf pieces and cotyledons
- Only germinating seeds
- Leaf pieces, cotyledons, stem segments, callus suspension cultures, and germinating seeds (correct)
Why are viruses not routinely used as vectors for gene transfer in plants?
Why are viruses not routinely used as vectors for gene transfer in plants?
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What is biolistics?
What is biolistics?
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What is electroporation?
What is electroporation?
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What are the genetic elements that constitute the T-DNA transfer machinery?
What are the genetic elements that constitute the T-DNA transfer machinery?
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Study Notes
- Agrobacterium-mediated transformation is a common method for plant genetic modification.
- Tumorigenic genes are removed from the bacterium during transformation.
- The binary vector system is a two-plasmid system that is effective for transforming plant cells.
- Leaf pieces, cotyledons, stem segments, callus suspension cultures, and germinating seeds can be used as explants for transformation.
- Viruses have been investigated as vectors for gene transfer in plants, but are not routinely used.
- Biolistics is the most effective and widely used method for generating commercial transgenic crops.
- Physico-chemical uptake of DNA by protoplasts and cells can be used to produce transgenic plants.
- Electroporation can induce pores in the protoplast membrane to allow for DNA uptake.
- T-DNA, Agrobacterium chromosomal virulence genes, and Ti plasmid virulence genes are the genetic elements that constitute the T-DNA transfer machinery.
- An efficient tissue culture system is required for the regeneration of transformant cells.
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