Plant Genetic Modification
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Questions and Answers

What is Agrobacterium-mediated transformation?

  • A common method for animal genetic modification
  • A method for plant genetic modification that involves the use of chemicals
  • A method for plant genetic modification that involves the use of bacteria (correct)
  • A method for plant genetic modification that involves the use of viruses
  • What is the binary vector system?

  • A three-plasmid system that is effective for transforming plant cells
  • A two-plasmid system that is effective for transforming animal cells
  • A one-plasmid system that is effective for transforming plant cells
  • A two-plasmid system that is effective for transforming plant cells (correct)
  • Which of the following can be used as explants for transformation?

  • Only stem segments and callus suspension cultures
  • Only leaf pieces and cotyledons
  • Only germinating seeds
  • Leaf pieces, cotyledons, stem segments, callus suspension cultures, and germinating seeds (correct)
  • Why are viruses not routinely used as vectors for gene transfer in plants?

    <p>Because they are not effective in transferring genes to plants</p> Signup and view all the answers

    What is biolistics?

    <p>A method for plant genetic modification that involves the use of high-velocity particles</p> Signup and view all the answers

    What is electroporation?

    <p>A method for plant genetic modification that involves the use of electricity</p> Signup and view all the answers

    What are the genetic elements that constitute the T-DNA transfer machinery?

    <p>T-DNA, Agrobacterium chromosomal virulence genes, and Ti plasmid virulence genes</p> Signup and view all the answers

    Study Notes

    • Agrobacterium-mediated transformation is a common method for plant genetic modification.
    • Tumorigenic genes are removed from the bacterium during transformation.
    • The binary vector system is a two-plasmid system that is effective for transforming plant cells.
    • Leaf pieces, cotyledons, stem segments, callus suspension cultures, and germinating seeds can be used as explants for transformation.
    • Viruses have been investigated as vectors for gene transfer in plants, but are not routinely used.
    • Biolistics is the most effective and widely used method for generating commercial transgenic crops.
    • Physico-chemical uptake of DNA by protoplasts and cells can be used to produce transgenic plants.
    • Electroporation can induce pores in the protoplast membrane to allow for DNA uptake.
    • T-DNA, Agrobacterium chromosomal virulence genes, and Ti plasmid virulence genes are the genetic elements that constitute the T-DNA transfer machinery.
    • An efficient tissue culture system is required for the regeneration of transformant cells.

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    Description

    Are you interested in plant genetic modification? Test your knowledge with this quiz on Agrobacterium-mediated transformation, binary vector systems, explants, and other methods for generating transgenic plants. Learn about the genetic elements involved in T-DNA transfer machinery and the tissue culture systems required for successful transformation. Sharpen your understanding of the latest techniques and technologies in plant biotechnology.

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