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Questions and Answers
What is the overall thickness of the plasma membrane as shown in the electron micrograph?
What is the overall thickness of the plasma membrane as shown in the electron micrograph?
Which of the following is a characteristic of phosphatidylcholine?
Which of the following is a characteristic of phosphatidylcholine?
Which component is part of a glycosylphosphatidylinositol (GPI)-anchored protein structure?
Which component is part of a glycosylphosphatidylinositol (GPI)-anchored protein structure?
Which fatty acid is associated with glycosylphosphatidylinositol (GPI)-anchored proteins?
Which fatty acid is associated with glycosylphosphatidylinositol (GPI)-anchored proteins?
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Which type of protein is linked to the membrane through a prenyl group?
Which type of protein is linked to the membrane through a prenyl group?
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What is the primary function of nucleosomes in the packaging of DNA?
What is the primary function of nucleosomes in the packaging of DNA?
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Which type of chromatin is characterized as being transcriptionally inactive?
Which type of chromatin is characterized as being transcriptionally inactive?
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What percentage of the DNA in the nucleus is typically composed of heterochromatin?
What percentage of the DNA in the nucleus is typically composed of heterochromatin?
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During mitosis, how does chromatin condense?
During mitosis, how does chromatin condense?
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What characterizes euchromatin within a cell?
What characterizes euchromatin within a cell?
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What is the primary function of peripheral proteins in a cell membrane?
What is the primary function of peripheral proteins in a cell membrane?
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Which component of phospholipids is variable and can influence its functions?
Which component of phospholipids is variable and can influence its functions?
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What distinguishes anchored proteins from peripheral proteins?
What distinguishes anchored proteins from peripheral proteins?
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Which property of phospholipid molecules contributes to their amphipathic nature?
Which property of phospholipid molecules contributes to their amphipathic nature?
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What role do ionic and hydrogen bonds play in the association of peripheral proteins with the membrane?
What role do ionic and hydrogen bonds play in the association of peripheral proteins with the membrane?
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What role does the signal peptide play in the process described?
What role does the signal peptide play in the process described?
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How are N-linked glycoproteins transported to the Golgi apparatus?
How are N-linked glycoproteins transported to the Golgi apparatus?
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What occurs within the lumens of the Golgi cisternae?
What occurs within the lumens of the Golgi cisternae?
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What is the role of the trans Golgi network (TGN) in the process?
What is the role of the trans Golgi network (TGN) in the process?
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What happens to the contents as they traverse from one Golgi cisterna to the next?
What happens to the contents as they traverse from one Golgi cisterna to the next?
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Flashcards
Integral Membrane Proteins
Integral Membrane Proteins
Proteins that are embedded in the lipid bilayer of membranes.
Phospholipids
Phospholipids
A class of lipids with two fatty acids linked to glycerol and a phosphate group.
Peripheral Proteins
Peripheral Proteins
Proteins attached to the membrane surface by noncovalent bonds.
Amphipathic Molecules
Amphipathic Molecules
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Head Group (Phospholipids)
Head Group (Phospholipids)
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Nucleus Size vs DNA Length
Nucleus Size vs DNA Length
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Nucleosome Structure
Nucleosome Structure
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Chromatin Types
Chromatin Types
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Euchromatin Activity
Euchromatin Activity
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Heterochromatin Features
Heterochromatin Features
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Phosphatidylcholine
Phosphatidylcholine
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Galactosylglyceride
Galactosylglyceride
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Glycosylphosphatidylinositol (GPI)
Glycosylphosphatidylinositol (GPI)
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Fatty acid–anchored proteins
Fatty acid–anchored proteins
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Prenyl lipid–anchored proteins
Prenyl lipid–anchored proteins
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Signal Recognition Particle (SRP)
Signal Recognition Particle (SRP)
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N-linked glycoproteins
N-linked glycoproteins
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Golgi apparatus
Golgi apparatus
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Cisternae
Cisternae
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Trans Golgi Network (TGN)
Trans Golgi Network (TGN)
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Study Notes
Plant Cells
- Plants are multicellular organisms with specialized cells
- All plant cells have a basic eukaryotic organization:
- Nucleus
- Cytoplasm
- Subcellular organelles
- Enclosed in a membrane
- Some structures (like the nucleus) might be lost during cell maturation, but all plant cells start with similar organelles
Biological Membranes
- All cells are enclosed by a membrane (plasma membrane or plasmalemma), separating the cytoplasm from the external environment
- The plasma membrane controls the transport of substances into and out of the cell
- Membranes define boundaries of specialized organelles
- Made up of a phospholipid bilayer with embedded proteins
- Phospholipids have hydrophilic heads and hydrophobic tails (amphipathic)
- Integral proteins are embedded in the bilayer, sometimes spanning the entire width
- Peripheral proteins are bound to the surface by non-covalent bonds
- Anchored proteins are bound to the membrane by lipid molecules
- The fluidity of the membrane is influenced by temperature. Unsaturated fatty acids help maintain fluidity at low temperatures
- Plastid membranes differ in their lipid composition, mainly containing glycosylglycerides instead of phospholipids.
The Nucleus
- Contains the majority of the genetic information in the cell (the nuclear genome)
- Enclosed by a double membrane (nuclear envelope)
- Contains nuclear pores for transport of macromolecules
- Contains chromatin (DNA and proteins) and nucleolus (ribosome synthesis)
- Sizes of plant nuclear genomes vary significantly
The Endoplasmic Reticulum (ER)
- A network of internal membranes creating sacs and tubules (cisternae)
- Continuous with the outer nuclear membrane
- Two types:
- Rough ER (RER) is studded with ribosomes involved in protein synthesis
- Smooth ER (SER) is involved in lipid synthesis and membrane assembly
Protein Synthesis
- Starts in the rough ER, where proteins destined for secretion enter the lumen
- Signal peptides direct the proteins through the ER.
- Glycosylation (adding sugars) occurs in the ER and Golgi.
- Subsequent processing, packaging, and transport of proteins are managed by the Golgi apparatus
The Golgi Apparatus
- A series of flattened sacs or cisternae (Golgi bodies/dictyosomes)
- Has a trans and cis face.
- Involved in processing, modifying, and packaging proteins and polysaccharides for secretion
- Modifies glycosylated proteins. Adds or removes sugars, creating different glycoproteins
- Involved in cell wall formation and secretion. Sends products to cell exterior or vacuoles.
The Central Vacuole
- Large, water-filled compartment in mature plant cells
- Surrounded by a vacuolar membrane (tonoplast)
- Stores water, solutes (inorganic ions, sugars, enzymes, secondary metabolites)
- Important for turgor pressure, structural support (in herbaceous plants)
- Involved in storage and degradation
Mitochondria and Chloroplasts
- Organelles involved in energy conversion
- Mitochondria: respiration (ATP synthesis from glucose)
- Chloroplasts: photosynthesis (glucose synthesis)
- Both contain their own DNA and ribosomes (are considered semi-autonomous)
- Double membranes, with inner membranes forming cristae in mitochondria and thylakoids in chloroplasts
Microbodies
- Small, spherical organelles with a single membrane
- Types: peroxisomes and glyoxysomes
- Peroxisomes: involved in oxidative reactions, breaking down H₂O₂
- Glyoxysomes: present in oil-storing seeds, convert stored fatty acids to sugars
Plasmodesmata
- Channels that traverse cell walls, connecting neighboring plant cells
- Allow communication and transport of molecules between cells
- Essential for symplastic transport (movement of substances through the interconnected cytoplasm)
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Description
Explore the fascinating world of plant cells and biological membranes in this quiz. Learn about the structure and function of eukaryotic plant cells, including their organelles, as well as the vital roles of membranes in cellular transport and structure. Test your knowledge and deepen your understanding of these essential biological concepts.