Piper Betle Extract Preparation
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Questions and Answers

What is the significance of the appearance of a brown color with a green ring or a blue/greenish-blue color in the Ferric Chloride and Folin-Ciocalteu tests?

It signifies the presence of phenols.

What is the purpose of preparing a standard gallic acid solution for calibration in the Folin-Ciocalteu method?

To determine the total phenolic content and express it as milligrams of gallic acid equivalents per gram of dry extract.

What is the purpose of performing qualitative phytochemical analysis of Piper betle methanol extract?

To gain valuable insights into the chemical profile of Piper betle leaves.

What is the difference between the results of Mayer's Test and Wagner's Test for alkaloids?

<p>Both tests showed the presence of alkaloids.</p> Signup and view all the answers

What is the significance of the absence of flavonoids in the Shinod's Test?

<p>It indicates that flavonoids are not present in the extract.</p> Signup and view all the answers

What is the purpose of expressing the total phenolic content in terms of milligrams of gallic acid equivalents per gram of dry extract?

<p>To quantify the amount of phenols present in the extract in a standardized unit.</p> Signup and view all the answers

What is the formula used to calculate the total phenolic content (TPC) from the absorbance and amount of gallic acid in mg/ml?

<p>y = 0.0024x + 0.6882</p> Signup and view all the answers

What units are used to express the total phenolic content (TPC) of the Piper betle leaf extract?

<p>mg GAE/g</p> Signup and view all the answers

What is the purpose of using the methanol extraction process in phytochemical analysis?

<p>To identify the presence of various phytochemicals</p> Signup and view all the answers

What type of phytochemicals are suggested to be responsible for the potential antibacterial effects of Piper betle leaves?

<p>Alkaloids, flavonoids, and phenols</p> Signup and view all the answers

What is the value of R² in the linear equation obtained from the calibration curve of gallic acid?

<p>0.9935</p> Signup and view all the answers

What is the concentration of gallic acid established from the calibration curve in mg/ml, represented by?

<p>c1</p> Signup and view all the answers

What is the ratio of Piper betle leaves to methanol used in the extraction process?

<p>1 gram leaves : 10 ml methanol</p> Signup and view all the answers

What is the purpose of applying a water bath to the extract?

<p>To further concentrate the extract by reducing its volume.</p> Signup and view all the answers

Which tests are employed to detect the presence of alkaloids in the extract?

<p>Mayer's and Wagner's tests</p> Signup and view all the answers

What is the indication of flavonoids presence in the extract using the alkaline reagent test and Shinod's test?

<p>A yellow color or a pink-scarlet color formation</p> Signup and view all the answers

What is the purpose of shade-drying the Piper betle leaves?

<p>To preserve the phytochemicals present in the leaves</p> Signup and view all the answers

What is the final volume of the concentrated extract after dilution with methanol?

<p>10 ml</p> Signup and view all the answers

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