18 Questions
What is the significance of the appearance of a brown color with a green ring or a blue/greenish-blue color in the Ferric Chloride and Folin-Ciocalteu tests?
It signifies the presence of phenols.
What is the purpose of preparing a standard gallic acid solution for calibration in the Folin-Ciocalteu method?
To determine the total phenolic content and express it as milligrams of gallic acid equivalents per gram of dry extract.
What is the purpose of performing qualitative phytochemical analysis of Piper betle methanol extract?
To gain valuable insights into the chemical profile of Piper betle leaves.
What is the difference between the results of Mayer's Test and Wagner's Test for alkaloids?
Both tests showed the presence of alkaloids.
What is the significance of the absence of flavonoids in the Shinod's Test?
It indicates that flavonoids are not present in the extract.
What is the purpose of expressing the total phenolic content in terms of milligrams of gallic acid equivalents per gram of dry extract?
To quantify the amount of phenols present in the extract in a standardized unit.
What is the formula used to calculate the total phenolic content (TPC) from the absorbance and amount of gallic acid in mg/ml?
y = 0.0024x + 0.6882
What units are used to express the total phenolic content (TPC) of the Piper betle leaf extract?
mg GAE/g
What is the purpose of using the methanol extraction process in phytochemical analysis?
To identify the presence of various phytochemicals
What type of phytochemicals are suggested to be responsible for the potential antibacterial effects of Piper betle leaves?
Alkaloids, flavonoids, and phenols
What is the value of R² in the linear equation obtained from the calibration curve of gallic acid?
0.9935
What is the concentration of gallic acid established from the calibration curve in mg/ml, represented by?
c1
What is the ratio of Piper betle leaves to methanol used in the extraction process?
1 gram leaves : 10 ml methanol
What is the purpose of applying a water bath to the extract?
To further concentrate the extract by reducing its volume.
Which tests are employed to detect the presence of alkaloids in the extract?
Mayer's and Wagner's tests
What is the indication of flavonoids presence in the extract using the alkaline reagent test and Shinod's test?
A yellow color or a pink-scarlet color formation
What is the purpose of shade-drying the Piper betle leaves?
To preserve the phytochemicals present in the leaves
What is the final volume of the concentrated extract after dilution with methanol?
10 ml
Follow the steps to prepare Piper betle extract, from taxonomically identifying leaves to grinding and mixing with methanol. Learn the proportions and resting time for proper extraction. Test your knowledge of this laboratory process.
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