Picric Acid Fixatives Quiz

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Which type of fixatives are made up of only 1 component substance?

Compound Fixatives

Microanatomical Fixatives

Metallic Fixatives

Aldehyde Fixatives (correct)

Which fixative is known for its corrosive nature?

Heidenhain’s susa

Zenker-formol (Helly’s) (correct)

Zenker's solution

Bouin’s solution

What is the primary component of Nuclear Fixatives?

Acetone

Absolute Ethyl alcohol

10% Formol saline

Glacial acetic acid (correct)

Which fixative is used to preserve chemical constituents of cells and tissues?

Newcomer’s fluid Signup and view all the answers

What is the pH of Nuclear Fixatives that preserve nuclear structures?

4.6 Signup and view all the answers

Which fixative is part of routine fixatives under Aldehyde Fixatives?

Formol saline Signup and view all the answers

What is the composition of commercial formalin in terms of formaldehyde gas by weight?

35-40% Signup and view all the answers

What is the advantage of buffering formalin to pH 7?

Prevents formation of dark brown artifact pigment granules Signup and view all the answers

What is the function of methanol in commercial formaldehyde?

Inhibits the formation of paraformaldehyde Signup and view all the answers

What happens to tissues fixed in unbuffered formalin that contain a high amount of blood?

Formation of dark brown artifact pigment granules Signup and view all the answers

What are Formalin pigments a result of?

Overfixation Signup and view all the answers

What is the chemical name for picric acid fixatives?

2,4,6-trinitrophenol Signup and view all the answers

Why is it recommended to keep picric acid moist during storage?

To maintain its fixative properties Signup and view all the answers

Which fixative is preferred for Masson’s trichrome staining for collagen, elastic, or connective tissue?

Brasil’s Alcoholic Picroformol Fixative Signup and view all the answers

What must be done to remove the yellow color caused by picric acid staining?

Add 50-70% ethanol Signup and view all the answers

What effect does Glacial Acetic Acid have on tissues?

It solidifies at 17ºC Signup and view all the answers

Why is Brasili’s Alcoholic Picroformol Fixative considered better than Bouin’s solution?

It causes tissues to swell less Signup and view all the answers

What is the main function of Methyl Alcohol in Wright's stain?

Fixing wet and dry smears Signup and view all the answers

Which alcohol is used in 70-100% concentrations and can cause RBC lysis at lower concentrations?

Ethyl Alcohol Signup and view all the answers

What is the recommended fixation time for Carnoy's Fluid?

1-3 hours Signup and view all the answers

Which fixative is suitable for preserving glycogen and capable of coagulating mucus?

Formalin Signup and view all the answers

What is the primary use of Newcomer's Fluid in fixing tissues?

Fixing mucopolysaccharides and nuclear proteins Signup and view all the answers

Which fixative is recommended for the nuclear preparation of sections?

Chrome-Osmium acetic acid Signup and view all the answers

Which fixative is recommended for cytoplasmic structures such as mitochondria?

Trichloroacetic acid (TCA) Signup and view all the answers

What effect does Trichloroacetic acid (TCA) have on tissues?

It causes marked swelling effect Signup and view all the answers

At what temperature is Acetone used as a fixative?

-50C to 40C Signup and view all the answers

Which fixative is commonly used for freeze substitution techniques?

Acetone Signup and view all the answers

For what purpose is Heat Fixation usually employed?

Fixing frozen tissue sections Signup and view all the answers

What is the primary use of Karnovsky’s paraformaldehyde-glutaraldehyde solution?

Fixation for Electron Microscopy Signup and view all the answers

Picric Acid Fixatives Quiz

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Questions and Answers

Which type of fixatives are made up of only 1 component substance?

Which fixative is known for its corrosive nature?

What is the primary component of Nuclear Fixatives?

Which fixative is used to preserve chemical constituents of cells and tissues?

What is the pH of Nuclear Fixatives that preserve nuclear structures?

Which fixative is part of routine fixatives under Aldehyde Fixatives?

What is the composition of commercial formalin in terms of formaldehyde gas by weight?

What is the advantage of buffering formalin to pH 7?

What is the function of methanol in commercial formaldehyde?

What happens to tissues fixed in unbuffered formalin that contain a high amount of blood?

What are Formalin pigments a result of?

What is the chemical name for picric acid fixatives?

Why is it recommended to keep picric acid moist during storage?

Which fixative is preferred for Masson’s trichrome staining for collagen, elastic, or connective tissue?

What must be done to remove the yellow color caused by picric acid staining?

What effect does Glacial Acetic Acid have on tissues?

Why is Brasili’s Alcoholic Picroformol Fixative considered better than Bouin’s solution?

What is the main function of Methyl Alcohol in Wright's stain?

Which alcohol is used in 70-100% concentrations and can cause RBC lysis at lower concentrations?

What is the recommended fixation time for Carnoy's Fluid?

Which fixative is suitable for preserving glycogen and capable of coagulating mucus?

What is the primary use of Newcomer's Fluid in fixing tissues?

Which fixative is recommended for the nuclear preparation of sections?

Which fixative is recommended for cytoplasmic structures such as mitochondria?

What effect does Trichloroacetic acid (TCA) have on tissues?

At what temperature is Acetone used as a fixative?

Which fixative is commonly used for freeze substitution techniques?

For what purpose is Heat Fixation usually employed?

What is the primary use of Karnovsky’s paraformaldehyde-glutaraldehyde solution?

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