Picric Acid Fixatives Quiz
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Questions and Answers

Which type of fixatives are made up of only 1 component substance?

  • Compound Fixatives
  • Microanatomical Fixatives
  • Metallic Fixatives
  • Aldehyde Fixatives (correct)
  • Which fixative is known for its corrosive nature?

  • Heidenhain’s susa
  • Zenker-formol (Helly’s) (correct)
  • Zenker's solution
  • Bouin’s solution
  • What is the primary component of Nuclear Fixatives?

  • Acetone
  • Absolute Ethyl alcohol
  • 10% Formol saline
  • Glacial acetic acid (correct)
  • Which fixative is used to preserve chemical constituents of cells and tissues?

    <p>Newcomer’s fluid</p> Signup and view all the answers

    What is the pH of Nuclear Fixatives that preserve nuclear structures?

    <p>4.6</p> Signup and view all the answers

    Which fixative is part of routine fixatives under Aldehyde Fixatives?

    <p>Formol saline</p> Signup and view all the answers

    What is the composition of commercial formalin in terms of formaldehyde gas by weight?

    <p>35-40%</p> Signup and view all the answers

    What is the advantage of buffering formalin to pH 7?

    <p>Prevents formation of dark brown artifact pigment granules</p> Signup and view all the answers

    What is the function of methanol in commercial formaldehyde?

    <p>Inhibits the formation of paraformaldehyde</p> Signup and view all the answers

    What happens to tissues fixed in unbuffered formalin that contain a high amount of blood?

    <p>Formation of dark brown artifact pigment granules</p> Signup and view all the answers

    What are Formalin pigments a result of?

    <p>Overfixation</p> Signup and view all the answers

    What is the chemical name for picric acid fixatives?

    <p>2,4,6-trinitrophenol</p> Signup and view all the answers

    Why is it recommended to keep picric acid moist during storage?

    <p>To maintain its fixative properties</p> Signup and view all the answers

    Which fixative is preferred for Masson’s trichrome staining for collagen, elastic, or connective tissue?

    <p>Brasil’s Alcoholic Picroformol Fixative</p> Signup and view all the answers

    What must be done to remove the yellow color caused by picric acid staining?

    <p>Add 50-70% ethanol</p> Signup and view all the answers

    What effect does Glacial Acetic Acid have on tissues?

    <p>It solidifies at 17ºC</p> Signup and view all the answers

    Why is Brasili’s Alcoholic Picroformol Fixative considered better than Bouin’s solution?

    <p>It causes tissues to swell less</p> Signup and view all the answers

    What is the main function of Methyl Alcohol in Wright's stain?

    <p>Fixing wet and dry smears</p> Signup and view all the answers

    Which alcohol is used in 70-100% concentrations and can cause RBC lysis at lower concentrations?

    <p>Ethyl Alcohol</p> Signup and view all the answers

    What is the recommended fixation time for Carnoy's Fluid?

    <p>1-3 hours</p> Signup and view all the answers

    Which fixative is suitable for preserving glycogen and capable of coagulating mucus?

    <p>Formalin</p> Signup and view all the answers

    What is the primary use of Newcomer's Fluid in fixing tissues?

    <p>Fixing mucopolysaccharides and nuclear proteins</p> Signup and view all the answers

    Which fixative is recommended for the nuclear preparation of sections?

    <p>Chrome-Osmium acetic acid</p> Signup and view all the answers

    Which fixative is recommended for cytoplasmic structures such as mitochondria?

    <p>Trichloroacetic acid (TCA)</p> Signup and view all the answers

    What effect does Trichloroacetic acid (TCA) have on tissues?

    <p>It causes marked swelling effect</p> Signup and view all the answers

    At what temperature is Acetone used as a fixative?

    <p>-50C to 40C</p> Signup and view all the answers

    Which fixative is commonly used for freeze substitution techniques?

    <p>Acetone</p> Signup and view all the answers

    For what purpose is Heat Fixation usually employed?

    <p>Fixing frozen tissue sections</p> Signup and view all the answers

    What is the primary use of Karnovsky’s paraformaldehyde-glutaraldehyde solution?

    <p>Fixation for Electron Microscopy</p> Signup and view all the answers

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