Picric Acid Fixatives Quiz
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Questions and Answers

Which type of fixatives are made up of only 1 component substance?

  • Compound Fixatives
  • Microanatomical Fixatives
  • Metallic Fixatives
  • Aldehyde Fixatives (correct)

Which fixative is known for its corrosive nature?

  • Heidenhain’s susa
  • Zenker-formol (Helly’s) (correct)
  • Zenker's solution
  • Bouin’s solution

What is the primary component of Nuclear Fixatives?

  • Acetone
  • Absolute Ethyl alcohol
  • 10% Formol saline
  • Glacial acetic acid (correct)

Which fixative is used to preserve chemical constituents of cells and tissues?

<p>Newcomer’s fluid (B)</p> Signup and view all the answers

What is the pH of Nuclear Fixatives that preserve nuclear structures?

<p>4.6 (B)</p> Signup and view all the answers

Which fixative is part of routine fixatives under Aldehyde Fixatives?

<p>Formol saline (A)</p> Signup and view all the answers

What is the composition of commercial formalin in terms of formaldehyde gas by weight?

<p>35-40% (A)</p> Signup and view all the answers

What is the advantage of buffering formalin to pH 7?

<p>Prevents formation of dark brown artifact pigment granules (D)</p> Signup and view all the answers

What is the function of methanol in commercial formaldehyde?

<p>Inhibits the formation of paraformaldehyde (D)</p> Signup and view all the answers

What happens to tissues fixed in unbuffered formalin that contain a high amount of blood?

<p>Formation of dark brown artifact pigment granules (C)</p> Signup and view all the answers

What are Formalin pigments a result of?

<p>Overfixation (B)</p> Signup and view all the answers

What is the chemical name for picric acid fixatives?

<p>2,4,6-trinitrophenol (B)</p> Signup and view all the answers

Why is it recommended to keep picric acid moist during storage?

<p>To maintain its fixative properties (D)</p> Signup and view all the answers

Which fixative is preferred for Masson’s trichrome staining for collagen, elastic, or connective tissue?

<p>Brasil’s Alcoholic Picroformol Fixative (B)</p> Signup and view all the answers

What must be done to remove the yellow color caused by picric acid staining?

<p>Add 50-70% ethanol (D)</p> Signup and view all the answers

What effect does Glacial Acetic Acid have on tissues?

<p>It solidifies at 17ºC (D)</p> Signup and view all the answers

Why is Brasili’s Alcoholic Picroformol Fixative considered better than Bouin’s solution?

<p>It causes tissues to swell less (A)</p> Signup and view all the answers

What is the main function of Methyl Alcohol in Wright's stain?

<p>Fixing wet and dry smears (C)</p> Signup and view all the answers

Which alcohol is used in 70-100% concentrations and can cause RBC lysis at lower concentrations?

<p>Ethyl Alcohol (A)</p> Signup and view all the answers

What is the recommended fixation time for Carnoy's Fluid?

<p>1-3 hours (B)</p> Signup and view all the answers

Which fixative is suitable for preserving glycogen and capable of coagulating mucus?

<p>Formalin (A)</p> Signup and view all the answers

What is the primary use of Newcomer's Fluid in fixing tissues?

<p>Fixing mucopolysaccharides and nuclear proteins (A)</p> Signup and view all the answers

Which fixative is recommended for the nuclear preparation of sections?

<p>Chrome-Osmium acetic acid (B)</p> Signup and view all the answers

Which fixative is recommended for cytoplasmic structures such as mitochondria?

<p>Trichloroacetic acid (TCA) (B)</p> Signup and view all the answers

What effect does Trichloroacetic acid (TCA) have on tissues?

<p>It causes marked swelling effect (D)</p> Signup and view all the answers

At what temperature is Acetone used as a fixative?

<p>-50C to 40C (D)</p> Signup and view all the answers

Which fixative is commonly used for freeze substitution techniques?

<p>Acetone (D)</p> Signup and view all the answers

For what purpose is Heat Fixation usually employed?

<p>Fixing frozen tissue sections (A)</p> Signup and view all the answers

What is the primary use of Karnovsky’s paraformaldehyde-glutaraldehyde solution?

<p>Fixation for Electron Microscopy (D)</p> Signup and view all the answers

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