Physics: Vectors and Vector operations

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Questions and Answers

In radioimmunoassay (RIA), what happens to the amount of radiolabeled antigen bound to a specific antibody as the concentration of unlabeled antigen increases?

  • It fluctuates randomly due to the radioactive decay of the labeled antigen.
  • It decreases as more unlabeled antigen competes for binding sites. (correct)
  • It increases proportionally with the concentration of unlabeled antigen.
  • It remains constant, unaffected by the unlabeled antigen.

Which of the following best describes the principle behind radioimmunoassay (RIA)?

  • Competitive binding of radiolabeled and unlabeled antigen to a high-affinity antibody. (correct)
  • Amplification of the signal produced by a small amount of antigen using enzymatic reactions.
  • Detection of specific antigens through their precipitation in a gel matrix.
  • Direct measurement of the electrical charge of an antibody-antigen complex.

In immunofluorescence microscopy, what property of fluorescent molecules is essential for their use as labels?

  • They absorb light at one wavelength and emit light at a longer wavelength. (correct)
  • They scatter light in proportion to the size of the molecule.
  • They conduct electricity when exposed to UV light.
  • They catalyze enzymatic reactions that produce visible light.

What is the primary purpose of using a UV light source in immunofluorescence microscopy?

<p>To excite the fluorophores attached to the antibodies. (B)</p> Signup and view all the answers

In the context of agglutination reactions, what are agglutinins?

<p>Antibodies that produce visible clumping of particulate antigens. (B)</p> Signup and view all the answers

How does the prozone effect inhibit agglutination reactions?

<p>By having an excess amount of antibody, preventing lattice formation. (D)</p> Signup and view all the answers

What is the fundamental principle underlying precipitation reactions between antigens and antibodies?

<p>The formation of a visible aggregate (lattice) from the interaction of soluble antigens and antibodies. (C)</p> Signup and view all the answers

For a precipitation reaction to occur effectively, what valency characteristics must the antibody and antigen possess?

<p>The antibody must be bivalent, and the antigen must be either bivalent or polyvalent. (A)</p> Signup and view all the answers

In a one-way mixed lymphocyte reaction (MLR), how is the stimulator cell population rendered incapable of proliferation?

<p>By treating them with mitomycin C or lethal x-irradiation. (B)</p> Signup and view all the answers

What is the fundamental purpose of performing a mixed lymphocyte reaction (MLR)?

<p>To assess the degree of T-cell proliferation in response to allogeneic stimulation. (A)</p> Signup and view all the answers

What is the role of the enzyme-conjugated secondary antibody in an enzyme-linked immunosorbent assay (ELISA)?

<p>To amplify the signal by binding to the primary antibody and catalyzing a color change in the substrate. (D)</p> Signup and view all the answers

In the context of single/radial immunodiffusion (Mancini method), how is the concentration of an antigen determined?

<p>By measuring the diameter of the precipitin ring that forms around the antigen well. (B)</p> Signup and view all the answers

What is the key principle behind double immunodiffusion (Ouchterlony method)?

<p>The simultaneous diffusion of both antigens and antibodies through a gel matrix to form precipitation patterns. (D)</p> Signup and view all the answers

In agglutination inhibition assays, what does the absence of agglutination indicate?

<p>A high concentration of the antigen in the sample, inhibiting agglutination. (D)</p> Signup and view all the answers

Which of the following best describes the purpose of a chromogenic substrate in an ELISA?

<p>To detect the presence of enzyme-conjugated antibodies through a color change. (A)</p> Signup and view all the answers

Flashcards

Radioimmunoassay (RIA)

A sensitive technique for detecting antigens or antibodies using radio-labeled antigens and antibodies.

Principle of RIA

The competitive binding of radiolabeled and unlabeled antigen to a high-affinity antibody.

Fluorescein

The most widely used label for immunofluorescence which absorbs blue light (490 nm) and emits intense yellow-green fluorescence (517 nm).

Rhodamine

An organic dye that absorbs in the yellow-green range (515 nm) and emits a deep red fluorescence (546 nm).

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Immunofluorescence

A technique where antibody molecules are tagged with a fluorescent dye or fluorochrome allow immune complexes to be detected by colored light emission.

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Enzyme-Linked Immunosorbent Assay (ELISA)

A type of enzyme-linked immunoassay where an enzyme conjugated with an antibody reacts with a colorless substrate to generate a colored reaction product.

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Agglutination

Antibody and antigen interaction that result in visible clumping.

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Agglutinins

Antibodies that produce agglutination reactions.

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Precipitation

Antibody and soluble antigen interacting in aqueous solution form a lattice that eventually develops into a visible precipitate.

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Mancini method

Single/Radial immunodiffusion

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Ouchterlony method

Double immunodiffusion.

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Mixed Lymphocyte Reaction (MLR)

A technique used to determine the immunogenicity of a compound.

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Study Notes

Physics

Vectors

  • Vectors can be added analytically by summing their respective components.
  • If vector $\overrightarrow{A} = A_x\hat{i} + A_y\hat{j} + A_z\hat{k}$ and vector $\overrightarrow{B} = B_x\hat{i} + B_y\hat{j} + B_z\hat{k}$, then $\overrightarrow{A} + \overrightarrow{B} = (A_x + B_x)\hat{i} + (A_y + B_y)\hat{j} + (A_z + B_z)\hat{k}$.

Scalar Product (Dot Product)

  • The dot product of two vectors is equal to the product of their magnitudes and the cosine of the angle between them: $\overrightarrow{A} \cdot \overrightarrow{B} = |\overrightarrow{A}||\overrightarrow{B}| \cos(\theta)$
  • The dot product can be calculated using components: $\overrightarrow{A} \cdot \overrightarrow{B} = (A_x \hat{i} + A_y \hat{j} + A_z \hat{k})\cdot(B_x \hat{i} + B_y \hat{j} + B_z \hat{k}) = A_xB_x + A_yB_y + A_zB_z$

Vector Product (Cross Product)

  • The cross product of two vectors is equal to the product of their magnitudes and the sine of the angle between them, multiplied by a unit vector normal to the plane containing the two vectors: $\overrightarrow{A} \times \overrightarrow{B} = |\overrightarrow{A}||\overrightarrow{B}| \sin(\theta) \hat{n}$
  • In component form: $\overrightarrow{A} \times \overrightarrow{B} = (A_x \hat{i} + A_y \hat{j} + A_z \hat{k})\times(B_x \hat{i} + B_y \hat{j} + B_z \hat{k}) = (A_yB_z - A_zB_y)\hat{i} + (A_zB_x - A_xB_z)\hat{j} + (A_xB_y - A_yB_x)\hat{k}$

Mixed Product

  • The mixed product of three vectors can be calculated as the determinant of a matrix formed by their components: $\overrightarrow{A} \cdot (\overrightarrow{B} \times \overrightarrow{C}) = \begin{vmatrix} A_x & A_y & A_z \ B_x & B_y & B_z \ C_x & C_y & C_z \end{vmatrix}$

Kinematics

Uniform Rectilinear Motion (MRU)

  • Position as a function of time: $x = x_0 + vt$

Uniformly Accelerated Rectilinear Motion (MRUA)

  • Position as a function of time: $x = x_0 + v_0t + \frac{1}{2}at^2$
  • Velocity as a function of time: $v = v_0 + at$
  • Velocity as a function of position: $v^2 = v_0^2 + 2a(x - x_0)$

Projectile Motion

  • Horizontal position: $x = x_0 + v_{0x}t$
  • Vertical position: $y = y_0 + v_{0y}t - \frac{1}{2}gt^2$
  • Horizontal velocity: $v_x = v_{0x}$
  • Vertical velocity: $v_y = v_{0y} - gt$

Uniform Circular Motion (MCU)

  • Arc length: $s = r\theta$
  • Linear velocity: $v = r\omega$
  • Linear acceleration: $a = r\alpha$
  • Angular velocity: $\omega = \frac{d\theta}{dt}$
  • Angular acceleration: $\alpha = \frac{d\omega}{dt}$

Dynamics

Newton's Laws

  • 1st Law: Net force is zero implies constant velocity: $\sum \overrightarrow{F} = 0 \Rightarrow \overrightarrow{v} = cte.$
  • 2nd Law: Net force equals mass times acceleration: $\sum \overrightarrow{F} = m\overrightarrow{a}$
  • 3rd Law: Action-reaction forces are equal and opposite: $\overrightarrow{F}{AB} = -\overrightarrow{F}{BA}$

Work and Energy

  • Work done by a force: $W = \overrightarrow{F} \cdot \overrightarrow{d} = |\overrightarrow{F}||\overrightarrow{d}| \cos(\theta)$
  • Kinetic energy: $K = \frac{1}{2}mv^2$
  • Gravitational potential energy: $U_g = mgh$
  • Elastic potential energy: $U_e = \frac{1}{2}kx^2$
  • Work-energy theorem: $W = \Delta K + \Delta U$
  • Power: $P = \frac{dW}{dt} = \overrightarrow{F} \cdot \overrightarrow{v}$

Impulse and Momentum

  • Momentum: $\overrightarrow{p} = m\overrightarrow{v}$
  • Impulse: $\overrightarrow{J} = \int \overrightarrow{F} dt = \Delta \overrightarrow{p}$
  • Conservation of momentum: $\sum \overrightarrow{p_i} = \sum \overrightarrow{p_f}$

Rotation

  • Moment of inertia: $I = \sum m_ir_i^2$
  • Kinetic energy of rotation: $K = \frac{1}{2}I\omega^2$
  • Torque: $\overrightarrow{\tau} = \overrightarrow{r} \times \overrightarrow{F} = I\overrightarrow{\alpha}$
  • Angular momentum: $L = I\omega$

Gravitation

  • Gravitational force: $\overrightarrow{F} = -G\frac{m_1m_2}{r^2}\hat{r}$
  • Gravitational potential energy: $U = -G\frac{m_1m_2}{r}$

Lecture 19: Lyapunov Stability

Autonomous System

  • The system is defined by $\dot{x} = f(x)$, where $x \in \mathbb{R}^n$ and $f: \mathbb{R}^n \rightarrow \mathbb{R}^n$.
  • $f$ must be locally Lipschitz, $f(0) = 0$, and $x_e = 0$ is an equilibrium point.

Definition of Stability

  • Stable: For every $\epsilon > 0$, there exists $\delta > 0$ such that if $||x(0)|| < \delta$, then $||x(t)|| < \epsilon$ for all $t \geq 0$.
  • Unstable: Not stable.
  • Asymptotically Stable: Stable, and there exists $\delta > 0$ such that if $||x(0)|| < \delta$, then $\lim_{t \to \infty} x(t) = 0$.
  • Globally Asymptotically Stable: Stable, and $\lim_{t \to \infty} x(t) = 0$ for all $x(0) \in \mathbb{R}^n$.

Lyapunov's Direct Method

Lyapunov Function

  • $V: \mathbb{R}^n \rightarrow \mathbb{R}$ is a Lyapunov function candidate if:
    • $V(0) = 0$
    • $V(x) > 0$ for all $x \neq 0$
  • $V(x)$ must be positive definite.

Theorem

  • If there exists a Lyapunov function candidate $V(x)$ such that $\dot{V}(x) \leq 0$ for all $x$, then $x_e = 0$ is stable.
  • If $\dot{V}(x) < 0$ for all $x \neq 0$, then $x_e = 0$ is asymptotically stable.
  • If $\dot{V}(x) < 0$ for all $x \neq 0$ and $V(x)$ is radially unbounded (i.e., $||x|| \rightarrow \infty \implies V(x) \rightarrow \infty$), then $x_e = 0$ is globally asymptotically stable.

Calculus Definitions

Common Functions

Polynomial

  • A polynomial function has the form: $f(x) = a_n x^n + a_{n-1} x^{n-1} +... + a_1 x + a_0$

Rational

  • A rational function is a ratio of two polynomials: $f(x) = \frac{P(x)}{Q(x)}$

Algebraic

  • Construction uses algebraic operations like addition, multiplication, and roots.

Trigonometric

  • Examples: $\sin(x), \cos(x), \tan(x)$ etc.

Exponential

  • Exponential functions have the form: $f(x) = a^x$ where a is a positive constant

Logarithmic

  • Logarithmic functions have the form: $f(x) = \log_a(x)$ where a is a positive constant

Limit Definition

  • $\lim_{x \to a} f(x) = L$ means that $f(x)$ can be made arbitrarily close to L when x is close to a but not equal to a.

Limit Laws

  • If $\lim_{x \to a} f(x)$ and $\lim_{x \to a} g(x)$ exist, then:
    • $\lim_{x \to a} [f(x) + g(x)] = \lim_{x \to a} f(x) + \lim_{x \to a} g(x)$
    • $\lim_{x \to a} [f(x) - g(x)] = \lim_{x \to a} f(x) - \lim_{x \to a} g(x)$
    • $\lim_{x \to a} [cf(x)] = c \lim_{x \to a} f(x)$
    • $\lim_{x \to a} [f(x)g(x)] = \lim_{x \to a} f(x) \cdot \lim_{x \to a} g(x)$
    • $\lim_{x \to a} \frac{f(x)}{g(x)} = \frac{\lim_{x \to a} f(x)}{\lim_{x \to a} g(x)}$, if $\lim_{x \to a} g(x) \neq 0$

Continuity

  • A function f is continuous at a number a if $\lim_{x \to a} f(x) = f(a)$

Conditions for Continuity

  • f must satisfy these conditions to be continuous at a:
    • $f(a)$ is defined
    • $\lim_{x \to a} f(x)$ exists
    • $\lim_{x \to a} f(x) = f(a)$

Types of Discontinuities

  • Removable: The limit exists, but $f(a)$ doesn't match the limit or is undefined.
    • Jump: Left and right limits exist but are unequal.
    • Infinite: The function has a vertical asymptote at x = a.

Derivatives

  • $f'(x) = \lim_{h \to 0} \frac{f(x+h) - f(x)}{h}$
    • Alternative Definition:
    • $f'(a) = \lim_{x \to a} \frac{f(x) - f(a)}{x - a}$

Basic Rules

Function Derivative
$f(x) = c$ $f'(x) = 0$
$f(x) = x^n$ $f'(x) = nx^{n-1}$
$f(x) = cf(x)$ $f'(x) = cf'(x)$
$f(x) = f(x) + g(x)$ $f'(x) = f'(x) + g'(x)$
$f(x) = f(x) - g(x)$ $f'(x) = f'(x) - g'(x)$

Product Rule

  • $(fg)' = f'g + fg'$

Quotient Rule

  • $(\frac{f}{g})' = \frac{f'g - fg'}{g^2}$

Chain Rule

  • $(f(g(x)))' = f'(g(x)) \cdot g'(x)$

Theorems

Intermediate Value Theorem

  • If f is continuous on $[a, b]$ and N is between $f(a)$ and $f(b)$, there exists c in $(a, b)$ such that $f(c) = N$.

Mean Value Theorem

  • If f is continuous on $[a, b]$ and differentiable on $(a, b)$, there exists c in $(a, b)$ such that $f'(c) = \frac{f(b) - f(a)}{b - a}$.

Static Electricity

Charging

Friction

  • Achieved when two neutral materials are rubbed together.
  • Objects gain opposite charges.
  • One material attracts electrons more strongly.

Conduction

  • A charged object makes contact with a neutral object.
  • Objects end up with the same charge.

Induction

  • A charged object is brought near, but doesn't touch, a neutral object.
  • Electrons rearrange within the neutral object (polarization).
  • Grounding after polarization results in an opposite charge on the neutral object.

Electric Fields

  • A region around a charged object exerts force on other charged objects.
  • Electric field lines point away from positives and towards negatives.
  • Closer field lines indicate a stronger field.

Electric Potential

  • Electric potential is the electric potential energy per unit charge, measured in volts (V).
  • Potential difference is voltage.
  • Electrons accelerate towards higher potential.

Capacitance

  • The ability of a conductor to store energy by separating charge.
  • Measured in farads (F).
  • Capacitance of a parallel plate capacitor: $C = \epsilon_0 * \frac{A}{d}$, where $\epsilon_0 = 8.85 \times 10^{-12} \frac{C^2}{Nm^2}$, A is plate area, and d is the distance between plates.

Current

  • The rate of flow of electric charge, measured in amperes (A).
  • Current is given by: $I = \frac{\Delta Q}{\Delta t}$
  • One ampere equals one coulomb per second.
  • Current flows in the direction of positive charge.
  • Electrons flow in the opposite direction.

Resistance

  • The opposition to current flow, measured in ohms ($\Omega$).
  • $R = \frac{\rho L}{A}$, where $\rho$ is resistivity, L is length, and A is cross-sectional area.

Ohm's Law

  • $V = IR$ where V is voltage, I is current, and R is resistance.

Electric Power

  • The rate at which electrical energy is converted to other forms.
  • Measured in watts (W).
  • $P = IV = I^2R = \frac{V^2}{R}$.

Bernoulli's Principle

  • States that an increase in fluid speed occurs simultaneously with a decrease in pressure or potential energy.

How Wings Generate Lift

  • Airfoil Shape: Curved upper surface, flatter lower surface.
  • Airflow Difference: Air travels a longer distance over the curved upper surface.
  • Speed and Pressure: Faster air (above) has lower pressure, and slower air (below) has higher pressure.
  • Lift Generation: Pressure difference creates an upward force (lift).

Applications

  • Airplanes use wings to generate lift.
  • Race cars use inverted wings to generate downward force.
  • Spray bottles draw fluid up a tube using low pressure.
  • Chimneys draw smoke up using low pressure at the top.

Bernoulli's Equation

  • $P + \frac{1}{2} \rho v^2 + \rho g h = \text{constant}$
  • $P$ is pressure, $\rho$ is density, $v$ is velocity, $g$ is gravity, $h$ is height.
  • Applies to ideal, steady-flow fluids.
  • Viscosity and turbulence may affect real-world accuracy.

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