Podcast
Questions and Answers
In radioimmunoassay (RIA), what happens to the amount of radiolabeled antigen bound to a specific antibody as the concentration of unlabeled antigen increases?
In radioimmunoassay (RIA), what happens to the amount of radiolabeled antigen bound to a specific antibody as the concentration of unlabeled antigen increases?
- It fluctuates randomly due to the radioactive decay of the labeled antigen.
- It decreases as more unlabeled antigen competes for binding sites. (correct)
- It increases proportionally with the concentration of unlabeled antigen.
- It remains constant, unaffected by the unlabeled antigen.
Which of the following best describes the principle behind radioimmunoassay (RIA)?
Which of the following best describes the principle behind radioimmunoassay (RIA)?
- Competitive binding of radiolabeled and unlabeled antigen to a high-affinity antibody. (correct)
- Amplification of the signal produced by a small amount of antigen using enzymatic reactions.
- Detection of specific antigens through their precipitation in a gel matrix.
- Direct measurement of the electrical charge of an antibody-antigen complex.
In immunofluorescence microscopy, what property of fluorescent molecules is essential for their use as labels?
In immunofluorescence microscopy, what property of fluorescent molecules is essential for their use as labels?
- They absorb light at one wavelength and emit light at a longer wavelength. (correct)
- They scatter light in proportion to the size of the molecule.
- They conduct electricity when exposed to UV light.
- They catalyze enzymatic reactions that produce visible light.
What is the primary purpose of using a UV light source in immunofluorescence microscopy?
What is the primary purpose of using a UV light source in immunofluorescence microscopy?
In the context of agglutination reactions, what are agglutinins?
In the context of agglutination reactions, what are agglutinins?
How does the prozone effect inhibit agglutination reactions?
How does the prozone effect inhibit agglutination reactions?
What is the fundamental principle underlying precipitation reactions between antigens and antibodies?
What is the fundamental principle underlying precipitation reactions between antigens and antibodies?
For a precipitation reaction to occur effectively, what valency characteristics must the antibody and antigen possess?
For a precipitation reaction to occur effectively, what valency characteristics must the antibody and antigen possess?
In a one-way mixed lymphocyte reaction (MLR), how is the stimulator cell population rendered incapable of proliferation?
In a one-way mixed lymphocyte reaction (MLR), how is the stimulator cell population rendered incapable of proliferation?
What is the fundamental purpose of performing a mixed lymphocyte reaction (MLR)?
What is the fundamental purpose of performing a mixed lymphocyte reaction (MLR)?
What is the role of the enzyme-conjugated secondary antibody in an enzyme-linked immunosorbent assay (ELISA)?
What is the role of the enzyme-conjugated secondary antibody in an enzyme-linked immunosorbent assay (ELISA)?
In the context of single/radial immunodiffusion (Mancini method), how is the concentration of an antigen determined?
In the context of single/radial immunodiffusion (Mancini method), how is the concentration of an antigen determined?
What is the key principle behind double immunodiffusion (Ouchterlony method)?
What is the key principle behind double immunodiffusion (Ouchterlony method)?
In agglutination inhibition assays, what does the absence of agglutination indicate?
In agglutination inhibition assays, what does the absence of agglutination indicate?
Which of the following best describes the purpose of a chromogenic substrate in an ELISA?
Which of the following best describes the purpose of a chromogenic substrate in an ELISA?
Flashcards
Radioimmunoassay (RIA)
Radioimmunoassay (RIA)
A sensitive technique for detecting antigens or antibodies using radio-labeled antigens and antibodies.
Principle of RIA
Principle of RIA
The competitive binding of radiolabeled and unlabeled antigen to a high-affinity antibody.
Fluorescein
Fluorescein
The most widely used label for immunofluorescence which absorbs blue light (490 nm) and emits intense yellow-green fluorescence (517 nm).
Rhodamine
Rhodamine
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Immunofluorescence
Immunofluorescence
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Enzyme-Linked Immunosorbent Assay (ELISA)
Enzyme-Linked Immunosorbent Assay (ELISA)
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Agglutination
Agglutination
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Agglutinins
Agglutinins
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Precipitation
Precipitation
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Mancini method
Mancini method
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Ouchterlony method
Ouchterlony method
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Mixed Lymphocyte Reaction (MLR)
Mixed Lymphocyte Reaction (MLR)
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Study Notes
Physics
Vectors
- Vectors can be added analytically by summing their respective components.
- If vector $\overrightarrow{A} = A_x\hat{i} + A_y\hat{j} + A_z\hat{k}$ and vector $\overrightarrow{B} = B_x\hat{i} + B_y\hat{j} + B_z\hat{k}$, then $\overrightarrow{A} + \overrightarrow{B} = (A_x + B_x)\hat{i} + (A_y + B_y)\hat{j} + (A_z + B_z)\hat{k}$.
Scalar Product (Dot Product)
- The dot product of two vectors is equal to the product of their magnitudes and the cosine of the angle between them: $\overrightarrow{A} \cdot \overrightarrow{B} = |\overrightarrow{A}||\overrightarrow{B}| \cos(\theta)$
- The dot product can be calculated using components: $\overrightarrow{A} \cdot \overrightarrow{B} = (A_x \hat{i} + A_y \hat{j} + A_z \hat{k})\cdot(B_x \hat{i} + B_y \hat{j} + B_z \hat{k}) = A_xB_x + A_yB_y + A_zB_z$
Vector Product (Cross Product)
- The cross product of two vectors is equal to the product of their magnitudes and the sine of the angle between them, multiplied by a unit vector normal to the plane containing the two vectors: $\overrightarrow{A} \times \overrightarrow{B} = |\overrightarrow{A}||\overrightarrow{B}| \sin(\theta) \hat{n}$
- In component form: $\overrightarrow{A} \times \overrightarrow{B} = (A_x \hat{i} + A_y \hat{j} + A_z \hat{k})\times(B_x \hat{i} + B_y \hat{j} + B_z \hat{k}) = (A_yB_z - A_zB_y)\hat{i} + (A_zB_x - A_xB_z)\hat{j} + (A_xB_y - A_yB_x)\hat{k}$
Mixed Product
- The mixed product of three vectors can be calculated as the determinant of a matrix formed by their components: $\overrightarrow{A} \cdot (\overrightarrow{B} \times \overrightarrow{C}) = \begin{vmatrix} A_x & A_y & A_z \ B_x & B_y & B_z \ C_x & C_y & C_z \end{vmatrix}$
Kinematics
Uniform Rectilinear Motion (MRU)
- Position as a function of time: $x = x_0 + vt$
Uniformly Accelerated Rectilinear Motion (MRUA)
- Position as a function of time: $x = x_0 + v_0t + \frac{1}{2}at^2$
- Velocity as a function of time: $v = v_0 + at$
- Velocity as a function of position: $v^2 = v_0^2 + 2a(x - x_0)$
Projectile Motion
- Horizontal position: $x = x_0 + v_{0x}t$
- Vertical position: $y = y_0 + v_{0y}t - \frac{1}{2}gt^2$
- Horizontal velocity: $v_x = v_{0x}$
- Vertical velocity: $v_y = v_{0y} - gt$
Uniform Circular Motion (MCU)
- Arc length: $s = r\theta$
- Linear velocity: $v = r\omega$
- Linear acceleration: $a = r\alpha$
- Angular velocity: $\omega = \frac{d\theta}{dt}$
- Angular acceleration: $\alpha = \frac{d\omega}{dt}$
Dynamics
Newton's Laws
- 1st Law: Net force is zero implies constant velocity: $\sum \overrightarrow{F} = 0 \Rightarrow \overrightarrow{v} = cte.$
- 2nd Law: Net force equals mass times acceleration: $\sum \overrightarrow{F} = m\overrightarrow{a}$
- 3rd Law: Action-reaction forces are equal and opposite: $\overrightarrow{F}{AB} = -\overrightarrow{F}{BA}$
Work and Energy
- Work done by a force: $W = \overrightarrow{F} \cdot \overrightarrow{d} = |\overrightarrow{F}||\overrightarrow{d}| \cos(\theta)$
- Kinetic energy: $K = \frac{1}{2}mv^2$
- Gravitational potential energy: $U_g = mgh$
- Elastic potential energy: $U_e = \frac{1}{2}kx^2$
- Work-energy theorem: $W = \Delta K + \Delta U$
- Power: $P = \frac{dW}{dt} = \overrightarrow{F} \cdot \overrightarrow{v}$
Impulse and Momentum
- Momentum: $\overrightarrow{p} = m\overrightarrow{v}$
- Impulse: $\overrightarrow{J} = \int \overrightarrow{F} dt = \Delta \overrightarrow{p}$
- Conservation of momentum: $\sum \overrightarrow{p_i} = \sum \overrightarrow{p_f}$
Rotation
- Moment of inertia: $I = \sum m_ir_i^2$
- Kinetic energy of rotation: $K = \frac{1}{2}I\omega^2$
- Torque: $\overrightarrow{\tau} = \overrightarrow{r} \times \overrightarrow{F} = I\overrightarrow{\alpha}$
- Angular momentum: $L = I\omega$
Gravitation
- Gravitational force: $\overrightarrow{F} = -G\frac{m_1m_2}{r^2}\hat{r}$
- Gravitational potential energy: $U = -G\frac{m_1m_2}{r}$
Lecture 19: Lyapunov Stability
Autonomous System
- The system is defined by $\dot{x} = f(x)$, where $x \in \mathbb{R}^n$ and $f: \mathbb{R}^n \rightarrow \mathbb{R}^n$.
- $f$ must be locally Lipschitz, $f(0) = 0$, and $x_e = 0$ is an equilibrium point.
Definition of Stability
- Stable: For every $\epsilon > 0$, there exists $\delta > 0$ such that if $||x(0)|| < \delta$, then $||x(t)|| < \epsilon$ for all $t \geq 0$.
- Unstable: Not stable.
- Asymptotically Stable: Stable, and there exists $\delta > 0$ such that if $||x(0)|| < \delta$, then $\lim_{t \to \infty} x(t) = 0$.
- Globally Asymptotically Stable: Stable, and $\lim_{t \to \infty} x(t) = 0$ for all $x(0) \in \mathbb{R}^n$.
Lyapunov's Direct Method
Lyapunov Function
- $V: \mathbb{R}^n \rightarrow \mathbb{R}$ is a Lyapunov function candidate if:
- $V(0) = 0$
- $V(x) > 0$ for all $x \neq 0$
- $V(x)$ must be positive definite.
Theorem
- If there exists a Lyapunov function candidate $V(x)$ such that $\dot{V}(x) \leq 0$ for all $x$, then $x_e = 0$ is stable.
- If $\dot{V}(x) < 0$ for all $x \neq 0$, then $x_e = 0$ is asymptotically stable.
- If $\dot{V}(x) < 0$ for all $x \neq 0$ and $V(x)$ is radially unbounded (i.e., $||x|| \rightarrow \infty \implies V(x) \rightarrow \infty$), then $x_e = 0$ is globally asymptotically stable.
Calculus Definitions
Common Functions
Polynomial
- A polynomial function has the form: $f(x) = a_n x^n + a_{n-1} x^{n-1} +... + a_1 x + a_0$
Rational
- A rational function is a ratio of two polynomials: $f(x) = \frac{P(x)}{Q(x)}$
Algebraic
- Construction uses algebraic operations like addition, multiplication, and roots.
Trigonometric
- Examples: $\sin(x), \cos(x), \tan(x)$ etc.
Exponential
- Exponential functions have the form: $f(x) = a^x$ where a is a positive constant
Logarithmic
- Logarithmic functions have the form: $f(x) = \log_a(x)$ where a is a positive constant
Limit Definition
- $\lim_{x \to a} f(x) = L$ means that $f(x)$ can be made arbitrarily close to L when x is close to a but not equal to a.
Limit Laws
- If $\lim_{x \to a} f(x)$ and $\lim_{x \to a} g(x)$ exist, then:
- $\lim_{x \to a} [f(x) + g(x)] = \lim_{x \to a} f(x) + \lim_{x \to a} g(x)$
- $\lim_{x \to a} [f(x) - g(x)] = \lim_{x \to a} f(x) - \lim_{x \to a} g(x)$
- $\lim_{x \to a} [cf(x)] = c \lim_{x \to a} f(x)$
- $\lim_{x \to a} [f(x)g(x)] = \lim_{x \to a} f(x) \cdot \lim_{x \to a} g(x)$
- $\lim_{x \to a} \frac{f(x)}{g(x)} = \frac{\lim_{x \to a} f(x)}{\lim_{x \to a} g(x)}$, if $\lim_{x \to a} g(x) \neq 0$
Continuity
- A function f is continuous at a number a if $\lim_{x \to a} f(x) = f(a)$
Conditions for Continuity
- f must satisfy these conditions to be continuous at a:
- $f(a)$ is defined
- $\lim_{x \to a} f(x)$ exists
- $\lim_{x \to a} f(x) = f(a)$
Types of Discontinuities
- Removable: The limit exists, but $f(a)$ doesn't match the limit or is undefined.
- Jump: Left and right limits exist but are unequal.
- Infinite: The function has a vertical asymptote at x = a.
Derivatives
- $f'(x) = \lim_{h \to 0} \frac{f(x+h) - f(x)}{h}$
- Alternative Definition:
- $f'(a) = \lim_{x \to a} \frac{f(x) - f(a)}{x - a}$
Basic Rules
Function | Derivative |
---|---|
$f(x) = c$ | $f'(x) = 0$ |
$f(x) = x^n$ | $f'(x) = nx^{n-1}$ |
$f(x) = cf(x)$ | $f'(x) = cf'(x)$ |
$f(x) = f(x) + g(x)$ | $f'(x) = f'(x) + g'(x)$ |
$f(x) = f(x) - g(x)$ | $f'(x) = f'(x) - g'(x)$ |
Product Rule
- $(fg)' = f'g + fg'$
Quotient Rule
- $(\frac{f}{g})' = \frac{f'g - fg'}{g^2}$
Chain Rule
- $(f(g(x)))' = f'(g(x)) \cdot g'(x)$
Theorems
Intermediate Value Theorem
- If f is continuous on $[a, b]$ and N is between $f(a)$ and $f(b)$, there exists c in $(a, b)$ such that $f(c) = N$.
Mean Value Theorem
- If f is continuous on $[a, b]$ and differentiable on $(a, b)$, there exists c in $(a, b)$ such that $f'(c) = \frac{f(b) - f(a)}{b - a}$.
Static Electricity
Charging
Friction
- Achieved when two neutral materials are rubbed together.
- Objects gain opposite charges.
- One material attracts electrons more strongly.
Conduction
- A charged object makes contact with a neutral object.
- Objects end up with the same charge.
Induction
- A charged object is brought near, but doesn't touch, a neutral object.
- Electrons rearrange within the neutral object (polarization).
- Grounding after polarization results in an opposite charge on the neutral object.
Electric Fields
- A region around a charged object exerts force on other charged objects.
- Electric field lines point away from positives and towards negatives.
- Closer field lines indicate a stronger field.
Electric Potential
- Electric potential is the electric potential energy per unit charge, measured in volts (V).
- Potential difference is voltage.
- Electrons accelerate towards higher potential.
Capacitance
- The ability of a conductor to store energy by separating charge.
- Measured in farads (F).
- Capacitance of a parallel plate capacitor: $C = \epsilon_0 * \frac{A}{d}$, where $\epsilon_0 = 8.85 \times 10^{-12} \frac{C^2}{Nm^2}$, A is plate area, and d is the distance between plates.
Current
- The rate of flow of electric charge, measured in amperes (A).
- Current is given by: $I = \frac{\Delta Q}{\Delta t}$
- One ampere equals one coulomb per second.
- Current flows in the direction of positive charge.
- Electrons flow in the opposite direction.
Resistance
- The opposition to current flow, measured in ohms ($\Omega$).
- $R = \frac{\rho L}{A}$, where $\rho$ is resistivity, L is length, and A is cross-sectional area.
Ohm's Law
- $V = IR$ where V is voltage, I is current, and R is resistance.
Electric Power
- The rate at which electrical energy is converted to other forms.
- Measured in watts (W).
- $P = IV = I^2R = \frac{V^2}{R}$.
Bernoulli's Principle
- States that an increase in fluid speed occurs simultaneously with a decrease in pressure or potential energy.
How Wings Generate Lift
- Airfoil Shape: Curved upper surface, flatter lower surface.
- Airflow Difference: Air travels a longer distance over the curved upper surface.
- Speed and Pressure: Faster air (above) has lower pressure, and slower air (below) has higher pressure.
- Lift Generation: Pressure difference creates an upward force (lift).
Applications
- Airplanes use wings to generate lift.
- Race cars use inverted wings to generate downward force.
- Spray bottles draw fluid up a tube using low pressure.
- Chimneys draw smoke up using low pressure at the top.
Bernoulli's Equation
- $P + \frac{1}{2} \rho v^2 + \rho g h = \text{constant}$
- $P$ is pressure, $\rho$ is density, $v$ is velocity, $g$ is gravity, $h$ is height.
- Applies to ideal, steady-flow fluids.
- Viscosity and turbulence may affect real-world accuracy.
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