Phlebotomy Procedures and Techniques
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Questions and Answers

What should be done if alcohol is not allowed to dry before venipuncture?

  • It may cause specimen hemolysis. (correct)
  • It improves patient comfort.
  • It ensures proper needle insertion.
  • It causes specimen contamination.
  • A needle with visible burrs or discoloration should be used for venipuncture.

    False

    What is the angle of needle entry recommended for venipuncture?

    15 to 30 degrees

    After drawing blood, apply direct __________ to the puncture site.

    <p>pressure</p> Signup and view all the answers

    When multiple specimens are required, what should you do after the first collection tube is filled?

    <p>Invert the tube to prevent clotting.</p> Signup and view all the answers

    Match the tube color with its primary use:

    <p>Red-top tube = Clotting for serum testing Gel-barrier tube = Serum separation Lavender-top tube = Complete blood count (CBC) Green-top tube = Plasma testing</p> Signup and view all the answers

    It is safe to recap, cut, or bend used needles before disposal.

    <p>False</p> Signup and view all the answers

    What should you do with the used needle and holder after venipuncture?

    <p>Dispose of them in a sharps container.</p> Signup and view all the answers

    What is the purpose of the tiny rubber sleeve in blood specimen collection?

    <p>To seal the blood when the tube is removed</p> Signup and view all the answers

    It is acceptable to repeatedly have the patient pump their fist to make the vein more prominent.

    <p>False</p> Signup and view all the answers

    What should be done after securing the tourniquet and selecting the best vein for puncture?

    <p>Swab the site with a sterile alcohol sponge in a circular motion.</p> Signup and view all the answers

    The ______ sleeve automatically closes to prevent leakage during blood specimen collection.

    <p>rubber</p> Signup and view all the answers

    Match the following tube types with their additives:

    <p>Red Top = No additives Green Top = Heparin Lavender Top = EDTA Blue Top = Sodium citrate</p> Signup and view all the answers

    How long should a tourniquet be left on the arm?

    <p>One minute</p> Signup and view all the answers

    It is recommended to leave the tourniquet on while the blood is drawn.

    <p>True</p> Signup and view all the answers

    What is the recommended action before removing the needle shield on a blood specimen needle?

    <p>Pull the safety shield back over the holder.</p> Signup and view all the answers

    What color is hemolyzed serum or plasma typically?

    <p>Pink or red</p> Signup and view all the answers

    Using a 21- to 22-gauge needle is recommended for routine blood collections.

    <p>True</p> Signup and view all the answers

    What should be done if there is air leakage around the needle during blood collection?

    <p>Replace the vacuum tube.</p> Signup and view all the answers

    Too much __________ in drawing blood may damage red cells.

    <p>pressure</p> Signup and view all the answers

    Which of the following is a recommended practice for preventing hemolysis when performing venipuncture?

    <p>Allowing the collection site to dry after cleaning</p> Signup and view all the answers

    Match the following steps to their purpose in preventing hemolysis:

    <p>Use a 21- to 22-gauge needle = Minimize damage to red blood cells Allow specimen to clot completely = Ensure accurate serum separation Collect using sterile equipment = Prevent contamination Be gentle when drawing blood = Reduce cell rupture</p> Signup and view all the answers

    How long should a specimen be allowed to clot before centrifugation?

    <p>30 to 60 minutes</p> Signup and view all the answers

    Using a blood pressure cuff instead of a tourniquet increases the risk of red blood cell damage.

    <p>False</p> Signup and view all the answers

    Study Notes

    Venipuncture Preparation and Technique

    • Iodine can contaminate specimens; avoid using it for certain tests.
    • Allow the puncture site to air dry after cleaning; drying with gauze is acceptable.
    • If alcohol doesn’t dry, it may lead to hemolysis, which can affect test results.
    • Perform visual inspection of the needle for burrs or discoloration before use; do not use damaged needles.
    • Anchor the vein and insert the needle at a 15 to 30-degree angle.

    Blood Collection Process

    • For single tube collection, release the tourniquet when the tube is filled, and remove it carefully.
    • In multiple sample collections, invert the first tube after filling to prevent clotting before inserting the next tube.
    • A tiny rubber sleeve prevents leakage when changing tubes; do not recap or reuse needles.
    • Use direct pressure on the puncture site after blood draw to minimize bleeding; prolonged bleeding over five minutes should be reported.

    Safety and Disposal

    • Lock the safety shield on the needle after use and dispose of in a sharps container.
    • Follow exposure control plans for disposal; do not bend or cut needles.

    Needle and Syringe Preparation

    • Prepare the needle holder and attach the safety needle securely.
    • Use caution while removing the needle shield and push collection tubes forward until the needle touches the stopper to maintain vacuum integrity.

    Patient Positioning and Tourniquet Use

    • Avoid having the patient pump their fist, which can alter results by shifting fluid concentrations.
    • Do not leave a tourniquet on for more than one minute to prevent discomfort and hemolysis.

    Puncture Site Preparation

    • Clean the puncture site using a sterile alcohol sponge in a circular motion from inside to outside.
    • Do not use iodine unless specified; hemolysis can occur if not handled correctly.

    Hemolysis Prevention

    • Use a 21- to 22-gauge needle for routine collections; a 23-gauge may be needed for elderly or pediatric patients.
    • Replace vacuum tubes if there is air leakage or loss of vacuum to prevent specimen integrity loss.
    • For difficult venipunctures, adopt gentle techniques to minimize red blood cell damage.

    Collection and Processing Guidelines

    • Avoid removing the needle while the tube is engaged; premature removal can cause hemolysis.
    • Draw blood evenly and gently to minimize damage to red blood cells.
    • Ensure the collection site completely dries after alcohol cleaning to prevent contamination.
    • Do not collect specimens through a hematoma and allow samples to clot for 30 to 60 minutes before centrifuging.

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    Description

    This quiz covers essential phlebotomy techniques, emphasizing the importance of dry puncture sites and the handling of iodine contamination. Understanding how to properly prepare a patient for venipuncture is crucial for avoiding complications such as specimen hemolysis.

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