Questions and Answers
What is the correct order of draw for blood collection tubes?
Why is the order of draw important?
To prevent contaminating later tubes with the additive from previous tubes.
What does the Serum Separator Tube (SST) contain?
Gold.
What does the Plasma Separator Tube (PST) contain?
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What tests are associated with Light Blue tubes?
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Red tubes contain additives.
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What do Gray tubes contain?
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What tests are performed with Yellow tubes?
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What is the primary function of Dark Green tubes?
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What tests are conducted with Lavender tubes?
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What is contained in Royal Blue tubes?
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Study Notes
Phlebotomy Order of Draw
- Order of draw sequence is crucial to prevent cross-contamination and inaccurate test results.
- Tubes in order: Sterile, Light Blue, Red, Gold, Light Green, Dark Green, Lavender, Gray, Yellow, Royal Blue.
Mnemonic Device
- Mnemonic: "Stop Light Red, Stay Put Green, Light Go" aids in recalling order of draw colors:
- Stop = Sterile
- Light = Light Blue
- Red
- Stay = SST (Gold)
- Put = PST (Light Green)
- Green = Dark Green
- Light = Lavender
- Go = Gray
Importance of Order of Draw
- Maintaining the correct order is vital to avoid contaminating subsequent tubes with additives from prior tubes.
- Errors in order can lead to erroneous test results.
Serum Separator Tube (SST)
- Gold tubes, containing a gel for clot separation, are used for serum tests.
Plasma Separator Tube (PST)
- Light Green tubes contain fibrinogen and are used for plasma tests.
Sterile Collection Bottles
- Must be collected first to ensure sterility for aerobic and anaerobic tests.
Light Blue Tubes
- Contain Sodium Citrate and are essential for coagulation tests like PT or PTT, as well as D-dimer tests.
Red Tubes
- Additive-free, used for tests such as HIV, blood type, drug levels, and Type and Screen.
- Red can be used as a discard tube when using a butterfly needle to ensure accurate filling of light blue tube.
Gold or Red/Gray/Speckled Tubes
- Function as Serum Separator Tubes (SST) containing glass particles or thixotropic gel.
- Utilized for serum tests including PSA, lipids, thyroid, and B12.
Light Green Tubes
- Known as Plasma Separating Tubes (PST) and contain Lithium heparin for chemistry tests.
Dark Green Tubes
- Contain sodium heparin, an anticoagulant, used for chemistry tests such as BMP and CMP.
Lavender Tubes
- Contain Ethylenediaminetetraacetic acid (EDTA) for hematology tests like CBC, ESR, HCT, RBC, WBC, and HGB.
Gray Tubes
- Contain Sodium Fluoride, which is antiglycolytic and is used for measuring blood glucose levels.
Yellow Tubes
- Contain Acid-citrate dextrose, used for tissue typing, paternity testing, and DNA studies.
Royal Blue Tubes
- Typically additive-free, used primarily for toxicology tests, but may contain traces of heparin.
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Description
Test your knowledge on the order of draw for phlebotomy with these comprehensive flashcards. This quiz covers the various tube colors, a useful mnemonic, and the importance of following the correct order. Enhance your understanding and retention of crucial information for successful blood collection.
