Pharmaceutical Microbiology: Cold Sterilization Methods

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Questions and Answers

What is the mechanism of action of ethylene oxide in sterilization?

Inhibition of DNA replication
Oxidation of cellular components
Denaturation of enzymes
Alkylation of hydroxyl and carboxyl groups of proteins and nucleic acids (correct)

What is the capacity of a gas autoclave used for ethylene oxide sterilization?

100-300 L (correct)
20-50 L
50-100 L
400-600 L

Which factor affects the efficacy of ethylene oxide sterilization?

Ionic strength
pH level
Concentration (correct)
Light exposure

What is the temperature range for effective ethylene oxide sterilization?

<p>45-63°C (B)</p> Signup and view all the answers

Which material does ethylene oxide penetrate during sterilization?

<p>Plastic and rubber (D)</p> Signup and view all the answers

Flashcards

How does ethylene oxide sterilize?

Ethylene oxide sterilizes by attaching (alkylating) to essential parts of proteins and nucleic acids (DNA, RNA), disrupting their function. This kills microorganisms.

What's the ideal temperature for ETO sterilization?

Ethylene oxide sterilization is typically done between 45°C and 63°C. This temperature range ensures effectiveness and prevents damage to materials.

How big are ETO gas autoclaves?

The volume of a gas autoclave for ethylene oxide sterilization is typically 100-300 liters. This allows for the efficient sterilization of a good amount of medical equipment.

What's the key factor in ETO effectiveness?

The concentration of ethylene oxide directly impacts its effectiveness. A higher concentration means faster sterilization.

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What can ETO sterilize?

Ethylene oxide can effectively penetrate materials like plastic and rubber. This makes it ideal for sterilizing many medical devices that can't withstand traditional high-heat sterilization.

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Study Notes

Mechanism of Action of Ethylene Oxide

Alkylating agent that denatures proteins and disrupts DNA, preventing microorganisms from reproducing
Reacts with amino, hydroxyl, and sulfhydryl groups in proteins and nucleic acids, rendering them inactive