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Questions and Answers
What is the primary function of a spectrophotometer?
What is the principle behind spectrophotometry?
What is the purpose of a monochromator in a spectrophotometer?
What type of spectrophotometer is used to measure the amount of light absorbed in the visible range of the spectrum?
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What is the purpose of a wavelength selector in a spectrophotometer?
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What is the application of spectrophotometry in various fields?
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What is the role of a collimator in a spectrophotometer?
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What is measured in spectrophotometry to determine the amount of a chemical substance?
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What is the purpose of a spectrophotometer in quantitative analysis?
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What is the use of a cuvette in spectrophotometry?
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Study Notes
What is a Spectrophotometer?
- A spectrophotometer measures the amount of light absorbed by a sample.
- It is used to measure the concentration of solutes in solution by measuring the amount of light absorbed by the solution in a cuvette.
Spectrophotometry
- Spectrophotometry is a method to measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through a sample solution.
- Each compound absorbs or transmits light over a certain range of wavelength.
- This measurement can also be used to measure the amount of a known chemical substance.
Spectrometer
- A spectrometer produces a desired range of wavelength of light.
- It consists of:
- A collimator (lens) that transmits a straight beam of light (photons).
- A monochromator (prism) that splits the light into several component wavelengths (spectrum).
- A wavelength selector (slit) that transmits only the desired wavelengths.
Applications of Spectrophotometers
- Spectrophotometry is one of the most useful methods of quantitative analysis in various fields, including:
- Chemistry
- Physics
- Biochemistry
- Material and chemical engineering
- Clinical applications
Types of Spectrophotometers
- Visible spectrophotometers
- UV/Visible spectrophotometers
- NIR (Near-Infrared) spectrophotometers
- NMR (Nuclear Magnetic Resonance) spectrophotometers
- Atomic Absorption Spectrophotometers
Using a Spectrophotometer in Lab
- A spectrophotometer is used in lab to measure the concentration of solutes in solution.
- A standard curve is plotted to determine the unknown concentration of a sample.
- A standard curve is a plot of absorbance vs. concentration of a known substance.
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Description
Learn about spectrophotometers, instruments that measure light absorption by a sample, and spectrophotometry, a method to measure chemical substance absorbance.
