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Questions and Answers
What is the primary function of a spectrophotometer?
What is the primary function of a spectrophotometer?
What is the principle behind spectrophotometry?
What is the principle behind spectrophotometry?
What is the purpose of a monochromator in a spectrophotometer?
What is the purpose of a monochromator in a spectrophotometer?
What type of spectrophotometer is used to measure the amount of light absorbed in the visible range of the spectrum?
What type of spectrophotometer is used to measure the amount of light absorbed in the visible range of the spectrum?
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What is the purpose of a wavelength selector in a spectrophotometer?
What is the purpose of a wavelength selector in a spectrophotometer?
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What is the application of spectrophotometry in various fields?
What is the application of spectrophotometry in various fields?
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What is the role of a collimator in a spectrophotometer?
What is the role of a collimator in a spectrophotometer?
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What is measured in spectrophotometry to determine the amount of a chemical substance?
What is measured in spectrophotometry to determine the amount of a chemical substance?
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What is the purpose of a spectrophotometer in quantitative analysis?
What is the purpose of a spectrophotometer in quantitative analysis?
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What is the use of a cuvette in spectrophotometry?
What is the use of a cuvette in spectrophotometry?
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Study Notes
What is a Spectrophotometer?
- A spectrophotometer measures the amount of light absorbed by a sample.
- It is used to measure the concentration of solutes in solution by measuring the amount of light absorbed by the solution in a cuvette.
Spectrophotometry
- Spectrophotometry is a method to measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through a sample solution.
- Each compound absorbs or transmits light over a certain range of wavelength.
- This measurement can also be used to measure the amount of a known chemical substance.
Spectrometer
- A spectrometer produces a desired range of wavelength of light.
- It consists of:
- A collimator (lens) that transmits a straight beam of light (photons).
- A monochromator (prism) that splits the light into several component wavelengths (spectrum).
- A wavelength selector (slit) that transmits only the desired wavelengths.
Applications of Spectrophotometers
- Spectrophotometry is one of the most useful methods of quantitative analysis in various fields, including:
- Chemistry
- Physics
- Biochemistry
- Material and chemical engineering
- Clinical applications
Types of Spectrophotometers
- Visible spectrophotometers
- UV/Visible spectrophotometers
- NIR (Near-Infrared) spectrophotometers
- NMR (Nuclear Magnetic Resonance) spectrophotometers
- Atomic Absorption Spectrophotometers
Using a Spectrophotometer in Lab
- A spectrophotometer is used in lab to measure the concentration of solutes in solution.
- A standard curve is plotted to determine the unknown concentration of a sample.
- A standard curve is a plot of absorbance vs. concentration of a known substance.
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Description
Learn about spectrophotometers, instruments that measure light absorption by a sample, and spectrophotometry, a method to measure chemical substance absorbance.
