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Questions and Answers
Which of the following is NOT a characteristic of dental plaque?
Which of the following is NOT a characteristic of dental plaque?
Why is a profound knowledge of periodontal microbiology necessary?
Why is a profound knowledge of periodontal microbiology necessary?
Which of the following is a hard deposit that forms by mineralization of dental plaque?
Which of the following is a hard deposit that forms by mineralization of dental plaque?
What is the primary etiologic factor for gingivitis and periodontitis?
What is the primary etiologic factor for gingivitis and periodontitis?
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Which of the following is NOT an ecosystem (niche) in the oral cavity?
Which of the following is NOT an ecosystem (niche) in the oral cavity?
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What is the significance of studying the formation, composition, and characteristics of dental plaque?
What is the significance of studying the formation, composition, and characteristics of dental plaque?
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Which of the following statements is TRUE about the role of bacteria in periodontal disease?
Which of the following statements is TRUE about the role of bacteria in periodontal disease?
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What is the significance of understanding the interactions between bacteria in dental plaque?
What is the significance of understanding the interactions between bacteria in dental plaque?
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What is the primary energy source for F. nucleatum?
What is the primary energy source for F. nucleatum?
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Which of the following bacteria are associated with pregnancy-associated gingivitis?
Which of the following bacteria are associated with pregnancy-associated gingivitis?
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What is the primary difference between the Nonspecific Plaque Hypothesis and the Specific Plaque Hypothesis?
What is the primary difference between the Nonspecific Plaque Hypothesis and the Specific Plaque Hypothesis?
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Which of the following bacteria is NOT associated with chronic periodontitis?
Which of the following bacteria is NOT associated with chronic periodontitis?
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Which type of plaque is associated with soft tissue destruction?
Which type of plaque is associated with soft tissue destruction?
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Which of the following bacterial species is most commonly associated with Localized Aggressive Periodontitis (LAP)?
Which of the following bacterial species is most commonly associated with Localized Aggressive Periodontitis (LAP)?
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What is the most effective way to control dental plaque biofilm?
What is the most effective way to control dental plaque biofilm?
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What is the primary characteristic of the bacterial composition in periodontal health?
What is the primary characteristic of the bacterial composition in periodontal health?
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What is the primary component of the intercellular matrix in dental plaque?
What is the primary component of the intercellular matrix in dental plaque?
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Which bacteria strain is predominantly found in the polysaccharide components of dental plaque?
Which bacteria strain is predominantly found in the polysaccharide components of dental plaque?
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What serves as the main source of inorganic materials in dental plaque?
What serves as the main source of inorganic materials in dental plaque?
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In what form do more than 99% of all bacteria on Earth live?
In what form do more than 99% of all bacteria on Earth live?
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Which of the following components is NOT part of the composition of the dental plaque intercellular matrix?
Which of the following components is NOT part of the composition of the dental plaque intercellular matrix?
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What is the difference in microbial composition between gingivitis and chronic periodontitis?
What is the difference in microbial composition between gingivitis and chronic periodontitis?
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What does the Red complex in periodontal disease primarily consist of?
What does the Red complex in periodontal disease primarily consist of?
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Which type of bacteria is primarily responsible for pregnancy-associated gingivitis?
Which type of bacteria is primarily responsible for pregnancy-associated gingivitis?
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What is the initial change observed in the microbial shift as health progresses to periodontitis?
What is the initial change observed in the microbial shift as health progresses to periodontitis?
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What is the best method for effective plaque control in periodontitis?
What is the best method for effective plaque control in periodontitis?
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Which cluster is primarily associated with bleeding on probing?
Which cluster is primarily associated with bleeding on probing?
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What characteristic do secondary colonizers generally exhibit?
What characteristic do secondary colonizers generally exhibit?
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Which statement about microbial interaction is accurate?
Which statement about microbial interaction is accurate?
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Which of the following species is typically associated with the tooth surface in dental plaque?
Which of the following species is typically associated with the tooth surface in dental plaque?
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What distinguishes the outer surface of mature plaque mass?
What distinguishes the outer surface of mature plaque mass?
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Which species is NOT typically found in the orange complex?
Which species is NOT typically found in the orange complex?
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How do oral bacterial species typically organize within subgingival plaque?
How do oral bacterial species typically organize within subgingival plaque?
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What is a characteristic of gram-positive species at the tooth surface?
What is a characteristic of gram-positive species at the tooth surface?
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What is the primary function of the pellicle in the oral cavity?
What is the primary function of the pellicle in the oral cavity?
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Which type of microorganisms primarily colonize the dental pellicle initially?
Which type of microorganisms primarily colonize the dental pellicle initially?
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What structural features do bacteria use to attach to the pellicle?
What structural features do bacteria use to attach to the pellicle?
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Which of the following is NOT a phase in the maturation of dental plaque?
Which of the following is NOT a phase in the maturation of dental plaque?
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What is coaggregation in dental plaque formation?
What is coaggregation in dental plaque formation?
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As dental plaque matures, the environment transitions from aerobic to what type of environment?
As dental plaque matures, the environment transitions from aerobic to what type of environment?
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Which of the following microorganisms is considered a secondary colonizer in dental plaque?
Which of the following microorganisms is considered a secondary colonizer in dental plaque?
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What classification system is used to categorize bacteria in the formation of dental plaque?
What classification system is used to categorize bacteria in the formation of dental plaque?
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Flashcards
Composition of Dental Plaque
Composition of Dental Plaque
Dental plaque consists of 70% microorganisms and 30% intercellular matrix.
Biofilm
Biofilm
A biofilm is a complex community of bacteria attached to a surface and embedded in a slime layer.
Bacterial Lifestyles
Bacterial Lifestyles
Bacteria can live free-floating (planktonic) or attached to surfaces, forming biofilms.
Intercellular Matrix Components
Intercellular Matrix Components
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Sources of Inorganic Materials
Sources of Inorganic Materials
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Tooth Deposits
Tooth Deposits
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Dental Plaque
Dental Plaque
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Calculus
Calculus
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Materia Alba
Materia Alba
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Periodontal Microbiota
Periodontal Microbiota
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Intraoral Ecosystems
Intraoral Ecosystems
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Bacterial Interactions
Bacterial Interactions
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Periodontal Treatment Modalities
Periodontal Treatment Modalities
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Pellicle formation
Pellicle formation
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Components of pellicle
Components of pellicle
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Initial Colonization
Initial Colonization
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Adhesins
Adhesins
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Fimbriae
Fimbriae
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Plaque maturation
Plaque maturation
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Secondary colonization
Secondary colonization
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Coaggregation
Coaggregation
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Microbial Clusters
Microbial Clusters
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Red Complex
Red Complex
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Green and Orange Complexes
Green and Orange Complexes
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Coaggregation Bridges
Coaggregation Bridges
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Competition in Biofilms
Competition in Biofilms
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Subgingival Plaque Architecture
Subgingival Plaque Architecture
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Dental Plaque Composition
Dental Plaque Composition
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Pathogen Interactions
Pathogen Interactions
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Subgingival Niche
Subgingival Niche
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Nucleatum
Nucleatum
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Supragingival Plaque
Supragingival Plaque
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Ecological Plaque Hypothesis
Ecological Plaque Hypothesis
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Marginal Plaque
Marginal Plaque
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Red Complex Bacteria
Red Complex Bacteria
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Subgingival Plaque
Subgingival Plaque
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Microbial Shifts
Microbial Shifts
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Specific Plaque Hypothesis
Specific Plaque Hypothesis
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Chronic Periodontitis
Chronic Periodontitis
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Gingivitis
Gingivitis
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P.intermedia
P.intermedia
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Mechanical Removal
Mechanical Removal
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Study Notes
Microbiology of Periodontal Diseases
- The periodontal microbiota is extremely complex
- It affects the host, the oral environment, and periodontal treatment
- Profound knowledge of periodontal microbiology is necessary
Learning Objectives
- Understand different tooth deposits, their formation, and disease implications
- Recognize different bacterial complexes and families involved in periodontal disease
- Comprehend bacterial interactions during various periodontal diseases
- Correlate periodontal treatments to the microbial reasons
Bacterial Types
-
Gram-negative bacteria
- Veillonella spp.
- Prevotella spp.
- Haemophilus spp.
- Neisseria spp.
- Fusobacterium
- Porphyromonas gingivalis
- Capnocytophaga spp.
-
Gram-positive bacteria
- Actinomyces spp.
- Actinomyces odontolyticus
- Streptococcus mitis
- Streptococcus sanguinis
- Streptococcus oralis
- Streptococcus salavanus
- Staphylococcus
- Streptococcus
- Clostratium spp
-
Other Bacteria
- Corroding rods
- Other Fusobacteria
- Aggregatibacter actinomycetemcomitans
- Porphyromonas gingivalis
Different Deposits on Teeth
- Dental plaque: A soft biofilm that adheres to tooth surfaces, including restorations
- Materia alba: Soft accumulations of bacteria and tissue cells; easily displaced
- Calculus: A hard deposit formed by mineralization of dental plaque
- Generally covered by a layer of unmineralized plaque
Dental Plaque = Biofilm
- Dental plaque is the primary etiologic factor for gingivitis and periodontitis
- It is a microbial community with a complex structure
- It increases resistance to antimicrobial agents due to limited diffusion of substances
Composition of Dental Plaque
- Microorganisms (70%)
- More than 500 bacterial strains; non-bacteria (e.g., yeasts, protozoa, viruses)
- Intercellular Matrix (30%) - Organic materials (e.g., glycoproteins, polysaccharides, albumin, lipids) - Inorganic materials (e.g., calcium, phosphorus, sodium, potassium, fluoride)
Organic Materials in Plaque
- Glycoproteins, derived from saliva and important for pellicle formation
- Polysaccharides, produced by bacteria (e.g., dextran)
- Albumin, derived from crevicular fluid
- Lipids, debris from disrupted cells and food
Inorganic Materials in Plaque
- Calcium and phosphorus
- Sodium, potassium, and fluoride
Bacterial Lifestyles
- Free-floating (planktonic)
- Attached to a surface forming a biofilm community
Biofilm Communities
- A biofilm is a well-organized community of bacteria adhering to surfaces
- Embedded in an extracellular slime layer
- More than 99% of bacteria on Earth are attached bacteria in biofilms
- Found on many medical and dental devices
Formation of Dental Plaque
- Different factors influence the location and rate of plaque formation (oral hygiene, diet, salivary composition, saliva flow rate)
- In the absence of oral hygiene, plaque accumulates until a balance between removal and formation is reached
- Plaque formation happens in 3 phases
- Formation of the pellicle on the tooth surface
- Initial colonization by bacteria
- Secondary colonization
- Plaque maturation
Initial Colonization of Plaque
- Bacteria are found within a few hours on the dental pellicle
- Predominantly gram-positive facultative microorganisms (e.g., Actinomyces viscosus, Streptococcus sanguis)
- Adherence to pellicle via specific molecules called adhesins on the bacterial surface
Secondary Colonization & Plaque Maturation
- Secondary colonizers are microorganisms that do not initially colonize clean surfaces
- e.g., Prevotella intermedia, Prevotella loescheii, Capnocytophaga spp., Fusobacterium nucleatum, Porphyromonas gingivalis
- They often adhere to pre-existing cells in the plaque
Coaggregation
- Interactions among different gram-positive bacteria
- Then between gram-positive and gram-negative species
- Between different gram-negative species in later stages
Microbial Ecology and Interactions
- Bacteria are classified into 5 clusters based on nutritional and atmospheric similarities/differences
- Interactions between clusters influence each other, related to specific periodontal states
- Species from certain clusters are found interacting with others, mainly well; certain clusters rarely observed without another cluster
Microorganisms Associated with Periodontal Diseases
- Periodontal health—Gram-positive, facultative species from genera Streptococcus and Actinomyces
- Gingivitis—equal proportions of gram-positive and gram-negative cocci, rods, and filaments
- Pregnancy-associated gingivitis—P. intermedia
- Chronic periodontitis—high percentages of aerobic bacterial species and P. gingivalis, P. intermedia, F. nucleatum, B. forsythus
- Localized aggressive periodontitis—A. actinomycetemcomitans is prominent with Herpesviruses (EBV-1 and HCMV) as potential factors
- Abscesses—F. nucleatum, P. intermedia, P. gingivalis, P. micros, and B. forsythus
Plaque Index
- A method for measuring plaque levels
- Plaque stained by tablets
- Calculation: (Number of sites with plaque / Number of sites evaluated) x 100
Controlling Dental Plaque Biofilm
- Mechanical removal is the most effective treatment
Plaque Hypothesis
- Nonspecific plaque hypothesis
- Specific plaque hypothesis
- Ecological plaque hypothesis
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Description
Test your knowledge on the characteristics of dental plaque and its role in periodontal diseases. This quiz covers essential topics like the microbiological aspects of gingivitis, plaque formation, and associated bacteria. Perfect for dental students and professionals seeking to refresh their understanding of periodontal microbiology.