PCR Reagents and Applications Quiz
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PCR Reagents and Applications Quiz

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@ArtisticOmaha

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Questions and Answers

What is the main function of hot-start DNA polymerases in PCR?

  • Reduce nonspecific amplification and increase yields (correct)
  • Enhance the specificity of the PCR reaction
  • Allow convenient room temperature setup for high-throughput applications
  • Increase the speed of DNA amplification

    • Which of the following components is NOT typically found in a standard PCR buffer?

  • KCl
  • Tris
  • MgCl2
  • EDTA (correct)

    • What is the primary role of MgCl2 in the PCR reaction?

  • It is the cofactor for thermostable DNA polymerases (correct)
  • It enhances the specificity of the PCR reaction
  • It facilitates primer binding
  • It aids in the denaturation of the DNA template

    • Which of the following reagents can be added to the PCR buffer to aid the reaction?

    <p>DMSO, BSA, glycerol, Tween-20</p> Signup and view all the answers

    What is the primary purpose of PCR optimization?

    <p>To enhance the specificity and yield of the desired amplicon</p> Signup and view all the answers

    Which of the following is NOT a common application of PCR?

    <p>Protein synthesis</p> Signup and view all the answers

    What is the primary role of $MgCl_2$ in the PCR reaction?

    <p>To provide a cofactor for DNA polymerase activity</p> Signup and view all the answers

    Which of the following DNA polymerases is known for its high fidelity in copying DNA?

    <p>$Pfu$ DNA polymerase</p> Signup and view all the answers

    What is the primary purpose of the denaturation step in the PCR cycle?

    <p>To separate the double-stranded DNA into single strands</p> Signup and view all the answers

    Which of the following is NOT a typical source of template DNA for PCR?

    <p>Bacterial cultures</p> Signup and view all the answers

    What is the primary advantage of using a hot-start DNA polymerase in PCR?

    <p>It reduces the risk of non-specific amplification</p> Signup and view all the answers

    What was the role of Taq polymerase in the Polymerase Chain Reaction (PCR)?

    <p>It was responsible for making many copies of a short length of DNA defined by primers.</p> Signup and view all the answers

    What is the purpose of Polymerase Chain Reaction (PCR)?

    <p>To amplify the number of copies of a specific region of DNA.</p> Signup and view all the answers

    Who invented the Polymerase Chain Reaction (PCR) and was awarded the 1993 Nobel Prize in Chemistry?

    <p>Kary Mullis</p> Signup and view all the answers

    What is the main outcome of using Polymerase Chain Reaction (PCR) in DNA analysis?

    <p>Creating an infinite number of DNA copies from one original copy.</p> Signup and view all the answers

    What is the significance of using a special DNA polymerase like Taq in PCR?

    <p>It prevents DNA degradation during high-temperature cycles.</p> Signup and view all the answers

    How does Polymerase Chain Reaction (PCR) aid in diagnosing illnesses?

    <p>By amplifying defective genes for diagnosis.</p> Signup and view all the answers

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