Podcast
Questions and Answers
What is the main function of hot-start DNA polymerases in PCR?
What is the main function of hot-start DNA polymerases in PCR?
- Reduce nonspecific amplification and increase yields (correct)
- Enhance the specificity of the PCR reaction
- Allow convenient room temperature setup for high-throughput applications
- Increase the speed of DNA amplification
Which of the following components is NOT typically found in a standard PCR buffer?
Which of the following components is NOT typically found in a standard PCR buffer?
- KCl
- Tris
- MgCl2
- EDTA (correct)
What is the primary role of MgCl2 in the PCR reaction?
What is the primary role of MgCl2 in the PCR reaction?
- It is the cofactor for thermostable DNA polymerases (correct)
- It enhances the specificity of the PCR reaction
- It facilitates primer binding
- It aids in the denaturation of the DNA template
Which of the following reagents can be added to the PCR buffer to aid the reaction?
Which of the following reagents can be added to the PCR buffer to aid the reaction?
What is the primary purpose of PCR optimization?
What is the primary purpose of PCR optimization?
Which of the following is NOT a common application of PCR?
Which of the following is NOT a common application of PCR?
What is the primary role of $MgCl_2$ in the PCR reaction?
What is the primary role of $MgCl_2$ in the PCR reaction?
Which of the following DNA polymerases is known for its high fidelity in copying DNA?
Which of the following DNA polymerases is known for its high fidelity in copying DNA?
What is the primary purpose of the denaturation step in the PCR cycle?
What is the primary purpose of the denaturation step in the PCR cycle?
Which of the following is NOT a typical source of template DNA for PCR?
Which of the following is NOT a typical source of template DNA for PCR?
What is the primary advantage of using a hot-start DNA polymerase in PCR?
What is the primary advantage of using a hot-start DNA polymerase in PCR?
What was the role of Taq polymerase in the Polymerase Chain Reaction (PCR)?
What was the role of Taq polymerase in the Polymerase Chain Reaction (PCR)?
What is the purpose of Polymerase Chain Reaction (PCR)?
What is the purpose of Polymerase Chain Reaction (PCR)?
Who invented the Polymerase Chain Reaction (PCR) and was awarded the 1993 Nobel Prize in Chemistry?
Who invented the Polymerase Chain Reaction (PCR) and was awarded the 1993 Nobel Prize in Chemistry?
What is the main outcome of using Polymerase Chain Reaction (PCR) in DNA analysis?
What is the main outcome of using Polymerase Chain Reaction (PCR) in DNA analysis?
What is the significance of using a special DNA polymerase like Taq in PCR?
What is the significance of using a special DNA polymerase like Taq in PCR?
How does Polymerase Chain Reaction (PCR) aid in diagnosing illnesses?
How does Polymerase Chain Reaction (PCR) aid in diagnosing illnesses?
Flashcards are hidden until you start studying
