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PCR Reagents and Applications Quiz

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Questions and Answers

What is the main function of hot-start DNA polymerases in PCR?

Reduce nonspecific amplification and increase yields (correct)

Enhance the specificity of the PCR reaction

Allow convenient room temperature setup for high-throughput applications

Increase the speed of DNA amplification

Which of the following components is NOT typically found in a standard PCR buffer?

KCl

Tris

MgCl2

EDTA (correct)

What is the primary role of MgCl2 in the PCR reaction?

It is the cofactor for thermostable DNA polymerases (correct)

It enhances the specificity of the PCR reaction

It facilitates primer binding

It aids in the denaturation of the DNA template

Which of the following reagents can be added to the PCR buffer to aid the reaction?

DMSO, BSA, glycerol, Tween-20 Signup and view all the answers

What is the primary purpose of PCR optimization?

To enhance the specificity and yield of the desired amplicon Signup and view all the answers

Which of the following is NOT a common application of PCR?

Protein synthesis Signup and view all the answers

What is the primary role of $MgCl_2$ in the PCR reaction?

To provide a cofactor for DNA polymerase activity Signup and view all the answers

Which of the following DNA polymerases is known for its high fidelity in copying DNA?

$Pfu$ DNA polymerase Signup and view all the answers

What is the primary purpose of the denaturation step in the PCR cycle?

To separate the double-stranded DNA into single strands Signup and view all the answers

Which of the following is NOT a typical source of template DNA for PCR?

Bacterial cultures Signup and view all the answers

What is the primary advantage of using a hot-start DNA polymerase in PCR?

It reduces the risk of non-specific amplification Signup and view all the answers

What was the role of Taq polymerase in the Polymerase Chain Reaction (PCR)?

It was responsible for making many copies of a short length of DNA defined by primers. Signup and view all the answers

What is the purpose of Polymerase Chain Reaction (PCR)?

To amplify the number of copies of a specific region of DNA. Signup and view all the answers

Who invented the Polymerase Chain Reaction (PCR) and was awarded the 1993 Nobel Prize in Chemistry?

Kary Mullis Signup and view all the answers

What is the main outcome of using Polymerase Chain Reaction (PCR) in DNA analysis?

Creating an infinite number of DNA copies from one original copy. Signup and view all the answers

What is the significance of using a special DNA polymerase like Taq in PCR?

It prevents DNA degradation during high-temperature cycles. Signup and view all the answers

How does Polymerase Chain Reaction (PCR) aid in diagnosing illnesses?

By amplifying defective genes for diagnosis. Signup and view all the answers