17 Questions
What is the main function of hot-start DNA polymerases in PCR?
Reduce nonspecific amplification and increase yields
Which of the following components is NOT typically found in a standard PCR buffer?
EDTA
What is the primary role of MgCl2 in the PCR reaction?
It is the cofactor for thermostable DNA polymerases
Which of the following reagents can be added to the PCR buffer to aid the reaction?
DMSO, BSA, glycerol, Tween-20
What is the primary purpose of PCR optimization?
To enhance the specificity and yield of the desired amplicon
Which of the following is NOT a common application of PCR?
Protein synthesis
What is the primary role of $MgCl_2$ in the PCR reaction?
To provide a cofactor for DNA polymerase activity
Which of the following DNA polymerases is known for its high fidelity in copying DNA?
$Pfu$ DNA polymerase
What is the primary purpose of the denaturation step in the PCR cycle?
To separate the double-stranded DNA into single strands
Which of the following is NOT a typical source of template DNA for PCR?
Bacterial cultures
What is the primary advantage of using a hot-start DNA polymerase in PCR?
It reduces the risk of non-specific amplification
What was the role of Taq polymerase in the Polymerase Chain Reaction (PCR)?
It was responsible for making many copies of a short length of DNA defined by primers.
What is the purpose of Polymerase Chain Reaction (PCR)?
To amplify the number of copies of a specific region of DNA.
Who invented the Polymerase Chain Reaction (PCR) and was awarded the 1993 Nobel Prize in Chemistry?
Kary Mullis
What is the main outcome of using Polymerase Chain Reaction (PCR) in DNA analysis?
Creating an infinite number of DNA copies from one original copy.
What is the significance of using a special DNA polymerase like Taq in PCR?
It prevents DNA degradation during high-temperature cycles.
How does Polymerase Chain Reaction (PCR) aid in diagnosing illnesses?
By amplifying defective genes for diagnosis.
Test your knowledge on Polymerase Chain Reaction (PCR) reagents and their applications in genetic fingerprinting, DNA mapping, molecular breeding, species identification, and more. Explore the concentrations of different components like Buffer, MgCl2, dNTPs, Primers, and DNA polymerase for various sample types and DNA templates.
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