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Questions and Answers
Which step of the Polymerase Chain Reaction (PCR) involves the separation of double-stranded DNA into single strands?
Which step of the Polymerase Chain Reaction (PCR) involves the separation of double-stranded DNA into single strands?
- Annealing
- Amplification
- Denaturation (correct)
- Extension
What is the purpose of annealing in the Polymerase Chain Reaction (PCR)?
What is the purpose of annealing in the Polymerase Chain Reaction (PCR)?
- To bind primers to the DNA flanking the target region (correct)
- To synthesize a new DNA strand
- To amplify specific segments of DNA
- To create millions of copies of a particular DNA region
At what temperature range does annealing occur in the Polymerase Chain Reaction (PCR)?
At what temperature range does annealing occur in the Polymerase Chain Reaction (PCR)?
- Around 30-45°C
- Around 90-105°C
- Around 50-65°C (correct)
- Around 70-85°C
What is the role of the DNA polymerase enzyme in the Polymerase Chain Reaction (PCR)?
What is the role of the DNA polymerase enzyme in the Polymerase Chain Reaction (PCR)?
What is the optimal temperature range for the extension step in the Polymerase Chain Reaction (PCR)?
What is the optimal temperature range for the extension step in the Polymerase Chain Reaction (PCR)?
Flashcards
Denaturation (PCR)
Denaturation (PCR)
The process of separating double-stranded DNA into single strands, achieved by heating the reaction mixture to a high temperature.
Annealing (PCR)
Annealing (PCR)
The step where short, single-stranded DNA molecules called primers bind to specific regions flanking the target DNA sequence.
Annealing Temperature
Annealing Temperature
The optimal temperature range for annealing in PCR, where primers bind efficiently to the DNA template.
DNA Polymerase (PCR)
DNA Polymerase (PCR)
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Extension Temperature
Extension Temperature
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Study Notes
Polymerase Chain Reaction (PCR) Steps
- Denaturation is the step that involves the separation of double-stranded DNA into single strands.
Annealing in PCR
- The purpose of annealing in PCR is to allow primers to bind to the template strands.
- Annealing occurs at a temperature range of 50-65°C.
Extension in PCR
- The role of the DNA polymerase enzyme in PCR is to synthesize new DNA strands by adding nucleotides to the primers.
- The optimal temperature range for the extension step in PCR is 72°C.
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