PCR Quiz
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Questions and Answers

Which step of the Polymerase Chain Reaction (PCR) involves the separation of double-stranded DNA into single strands?

  • Annealing
  • Amplification
  • Denaturation (correct)
  • Extension
  • What is the purpose of annealing in the Polymerase Chain Reaction (PCR)?

  • To bind primers to the DNA flanking the target region (correct)
  • To synthesize a new DNA strand
  • To amplify specific segments of DNA
  • To create millions of copies of a particular DNA region
  • At what temperature range does annealing occur in the Polymerase Chain Reaction (PCR)?

  • Around 30-45°C
  • Around 90-105°C
  • Around 50-65°C (correct)
  • Around 70-85°C
  • What is the role of the DNA polymerase enzyme in the Polymerase Chain Reaction (PCR)?

    <p>To synthesize a new DNA strand</p> Signup and view all the answers

    What is the optimal temperature range for the extension step in the Polymerase Chain Reaction (PCR)?

    <p>Around 70-85°C</p> Signup and view all the answers

    Study Notes

    Polymerase Chain Reaction (PCR) Steps

    • Denaturation is the step that involves the separation of double-stranded DNA into single strands.

    Annealing in PCR

    • The purpose of annealing in PCR is to allow primers to bind to the template strands.
    • Annealing occurs at a temperature range of 50-65°C.

    Extension in PCR

    • The role of the DNA polymerase enzyme in PCR is to synthesize new DNA strands by adding nucleotides to the primers.
    • The optimal temperature range for the extension step in PCR is 72°C.

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    Description

    Test your knowledge of the Polymerase Chain Reaction (PCR) with this quiz! Learn about the key steps involved in this molecular biology technique used to amplify specific DNA segments.

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