5 Questions
Which step of the Polymerase Chain Reaction (PCR) involves the separation of double-stranded DNA into single strands?
Denaturation
What is the purpose of annealing in the Polymerase Chain Reaction (PCR)?
To bind primers to the DNA flanking the target region
At what temperature range does annealing occur in the Polymerase Chain Reaction (PCR)?
Around 50-65°C
What is the role of the DNA polymerase enzyme in the Polymerase Chain Reaction (PCR)?
To synthesize a new DNA strand
What is the optimal temperature range for the extension step in the Polymerase Chain Reaction (PCR)?
Around 70-85°C
Study Notes
Polymerase Chain Reaction (PCR) Steps
- Denaturation is the step that involves the separation of double-stranded DNA into single strands.
Annealing in PCR
- The purpose of annealing in PCR is to allow primers to bind to the template strands.
- Annealing occurs at a temperature range of 50-65°C.
Extension in PCR
- The role of the DNA polymerase enzyme in PCR is to synthesize new DNA strands by adding nucleotides to the primers.
- The optimal temperature range for the extension step in PCR is 72°C.
Test your knowledge of the Polymerase Chain Reaction (PCR) with this quiz! Learn about the key steps involved in this molecular biology technique used to amplify specific DNA segments.
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