PCR Quiz
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Questions and Answers

What year was the polymerase chain reaction (PCR) invented?

  • 1976
  • 1983 (correct)
  • 1993
  • 2000

    • Who was jointly awarded the Nobel Prize in Chemistry in 1993 for their work related to PCR?

  • Rosalind Franklin and Maurice Wilkins
  • Kary Mullis and Michael Smith (correct)
  • Francis Crick and James Watson
  • Barbara McClintock and Thomas Hunt Morgan

    • What is the main purpose of PCR?

  • To create new DNA sequences
  • To make millions to billions of copies of a specific DNA sample rapidly (correct)
  • To identify protein structures
  • To analyze RNA samples

    • In what field is PCR now a common and often indispensable technique used?

    <p>Biomedical research</p> Signup and view all the answers

    What do the majority of PCR methods rely on?

    <p>Exponentially amplifying DNA sequences in a series of cycles of temperature changes</p> Signup and view all the answers

    What is the main purpose of molecular cloning?

    <p>To assemble recombinant DNA molecules and direct their replication within host organisms</p> Signup and view all the answers

    What does the term 'cloning' refer to in molecular cloning?

    <p>Replication of one molecule to produce a population of cells with identical DNA molecules</p> Signup and view all the answers

    What is the source of DNA used in molecular cloning?

    <p>Two different organisms: the species that is the source of the DNA to be cloned and the species that will serve as the living host for replication</p> Signup and view all the answers

    What is the initial step in a conventional molecular cloning experiment?

    <p>Treating the DNA with enzymes to generate smaller DNA fragments</p> Signup and view all the answers

    What is the role of vector DNA in molecular cloning?

    <p>To combine with DNA fragments to generate recombinant DNA molecules</p> Signup and view all the answers

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