PCR Cycle Quiz

Polymerase Chain Reaction (PCR)

• The purpose of PCR is to amplify specific DNA sequences, generating millions of copies of a target DNA molecule.

Denaturation Step of PCR

• During denaturation, the double-stranded DNA is heated to a high temperature (94-96°C), causing the hydrogen bonds between the strands to break, resulting in single-stranded DNA.

Annealing Step of PCR

• During annealing, the temperature is lowered to allow primers to bind specifically to the target DNA sequences, ensuring that the DNA polymerase extends the correct sequence.

Extension Step of PCR

• DNA polymerase reads the template DNA strand and adds nucleotides to the primer, synthesizing a new DNA strand complementary to the template.

Gel Electrophoresis

• The main principle behind gel electrophoresis is the separation of DNA molecules based on their size and charge, using an electric current to move them through a gel matrix, with smaller molecules moving faster than larger ones.

Test your knowledge of PCR (polymerase chain reaction) with this quiz that covers the three main steps in the PCR cycle. Sharpen your understanding of this common laboratory technique used to amplify specific regions of DNA for various applications in research and forensic science.