5 Questions
What is the purpose of the Polymerase Chain Reaction (PCR)?
To make many copies of a particular region of DNA
What happens during the denaturation step of PCR?
DNA double strands are separated into single strands
What occurs during the annealing step of PCR?
The temperature is lowered to allow primers to bind to specific target sequences
What is the role of DNA polymerase in the extension step of PCR?
Adds nucleotides to the primers, synthesizing new DNA strands
What is the main principle behind gel electrophoresis?
Using electrical current to move DNA in a gel
Study Notes
Polymerase Chain Reaction (PCR)
- The purpose of PCR is to amplify specific DNA sequences, generating millions of copies of a target DNA molecule.
Denaturation Step of PCR
- During denaturation, the double-stranded DNA is heated to a high temperature (94-96°C), causing the hydrogen bonds between the strands to break, resulting in single-stranded DNA.
Annealing Step of PCR
- During annealing, the temperature is lowered to allow primers to bind specifically to the target DNA sequences, ensuring that the DNA polymerase extends the correct sequence.
Extension Step of PCR
- DNA polymerase reads the template DNA strand and adds nucleotides to the primer, synthesizing a new DNA strand complementary to the template.
Gel Electrophoresis
- The main principle behind gel electrophoresis is the separation of DNA molecules based on their size and charge, using an electric current to move them through a gel matrix, with smaller molecules moving faster than larger ones.
Test your knowledge of PCR (polymerase chain reaction) with this quiz that covers the three main steps in the PCR cycle. Sharpen your understanding of this common laboratory technique used to amplify specific regions of DNA for various applications in research and forensic science.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
