PCR Application in Dermatophyte Identification

Keratinophilic fungi that infect keratinized tissues (skin, hair, and nails) of humans and animals, causing dermatophytosis.

An infection of the keratinized tissues caused by dermatophytes.

Traditional methods for identifying dermatophytes based on visible characteristics (microscopy and in-vitro culture).

Arbitrarily Primed PCR, a nucleic acid amplification technique to rapidly and precisely identify dermatophyte fungi.

Methods like PCR that make many copies of a specific piece of DNA to reveal characteristics of the origin.

Fungi that have a specific preference for keratin, a protein component of skin, hair, and nails.

The three main genera of dermatophyte fungi.

Primers that bind to various sections of DNA, allowing for the analysis of diversity in microbial species, like dermatophytes in this case.

A fungus that infects the skin, hair, or nails.

A fungal skin infection caused by dermatophytes, often called ringworm.

A single species of fungus in the genus Epidermophyton.

Fungi genera that cause dermatophytosis with various species.

Pathogenic fungus species in the Trichophyton genus, with some variants.

A molecular technique used to identify dermatophyte isolates quickly.

Visible signs and symptoms of a disease, helping to distinguish between similar dermatophytosis.

Technology used to enhance the speed and quality of diagnosing dermatophyte infections, like "AP-PCR"

A DNA amplification technique for quickly identifying dermatophytes without needing to know the exact DNA sequence.

Primers that bind to various locations on a DNA strand, enabling analysis of diversity in genetic material.

Traditional ways of identifying dermatophytes based on observable traits like growth on culture media.

The temperature at which primers bind to the DNA template.

Controlling the degree of primer matching to the template DNA sequence in a PCR reaction.

The process of determining the specific type of fungi causing skin, hair, or nail infections.

Different growth mediums for cultivating microorganisms, like dermatophytes.

A nucleic acid amplification technique to rapidly and precisely identify dermatophyte fungi.

A DNA amplification technique used to identify dermatophytes, focusing on their genetic makeup rather than visible traits.

The genetic traits of an organism, distinct from its observable physical traits (phenotype).

Primers that bind to various sections of DNA, allowing for the analysis of diversity in microbial species.

The physical traits of an organism, like size, shape, or color.

Fungi infecting keratinized tissues (skin, hair, nails) in humans.

Unique DNA sequences visible after amplification with random primers, distinguishing one fungus from another

Short DNA sequences that can attach to various parts of a DNA strand in AP-PCR, amplifying different sections.

Determining the order of DNA building blocks (nucleotides) in a specific DNA fragment, to study specific gene regions.

Table showing DNA products (in kilobases) obtained from different dermatophyte species using various random primers.

Specific species of dermatophyte fungi.

The process of identifying fungi that infect the skin, hair, and nails (dermatophytes).

Different random primers used for amplification of fungal DNA in AP-PCR.

Trichophyton ajelloi is identi®able using OPAA11 in AP-PCR.

Trichophyton concentricum is identi®able using OPD18 in AP-PCR.

Trichophyton equinum var.autotrophicum is identi®able using OPAA17 in AP-PCR.

AP-PCR markers like OPAA17, OPD18, OPAA17, OPU15, show it's non-identifiable.

Identi®cation by AP-PCR methods for various T. mentagrophytes variants are different, using various markers.

Microsporum audouinii is identi®able using AP-PCR markers OPAA11, OPD18, OPAA17, and OPU15

Various Dermatophyte species are identi®ed with varying results based on AP-PCR markers like OPAA11, OPD18, OPAA17, and OPU15.