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Questions and Answers
In the context of genetic engineering, what is the primary role of a vector?
In the context of genetic engineering, what is the primary role of a vector?
- To directly modify the genome of the host organism.
- To carry the desired DNA into a host cell for cloning. (correct)
- To induce tumor formation in plant cells.
- To provide resistance against antibiotics for the host cell.
Agrobacterium tumefaciens is LEAST likely to cause tumor formation in which type of plant:
Agrobacterium tumefaciens is LEAST likely to cause tumor formation in which type of plant:
- Sunflower
- Corn (correct)
- Soybean
- Tomato
What is the main function of the 'rop' gene in a plasmid?
What is the main function of the 'rop' gene in a plasmid?
- To initiate tumor formation in plant cells.
- To code for antibiotic resistance.
- To control the replication of the plasmid. (correct)
- To facilitate the transfer of T-DNA into the host cell.
What is the correct term to describe DNA from one organism that is inserted into another organism using a vector?
What is the correct term to describe DNA from one organism that is inserted into another organism using a vector?
What is the role of T-DNA in Agrobacterium-mediated transformation?
What is the role of T-DNA in Agrobacterium-mediated transformation?
What is the process by which Agrobacterium tumefaciens transfers T-DNA into a host plant cell?
What is the process by which Agrobacterium tumefaciens transfers T-DNA into a host plant cell?
What is the purpose of using a host cell in DNA cloning?
What is the purpose of using a host cell in DNA cloning?
Which of the following best describes the function of the Ti plasmid in Agrobacterium tumefaciens?
Which of the following best describes the function of the Ti plasmid in Agrobacterium tumefaciens?
In the context of pBR322, what do the resistant genes confer to the host cell?
In the context of pBR322, what do the resistant genes confer to the host cell?
What happens to the T-DNA after it is transferred into the host plant cell?
What happens to the T-DNA after it is transferred into the host plant cell?
Flashcards
Antibiotic Resistance Gene
Antibiotic Resistance Gene
A gene in PBR322 that provides resistance to ampicillin and tetracycline.
ROP Codes
ROP Codes
Codes for proteins involved in plasmid replication.
Desired/Foreign DNA
Desired/Foreign DNA
DNA from one organism inserted into another, combined with a vector DNA.
Host Cell
Host Cell
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Vectors in Cloning
Vectors in Cloning
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Agrobacterium tumefaciens
Agrobacterium tumefaciens
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Ti Plasmid
Ti Plasmid
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T-DNA
T-DNA
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Study Notes
- In PBR322, resistant genes for ampicillin and tetracycline are present.
- Rop codes for the protein involved in the replication of plasmid.
- Desired DNA/Foreign DNA: DNA transferred from one organism into another by combining it with the vector DNA.
- Host cell (organism): The cell or organism used for DNA cloning.
- Hosts can be plant, animal, bacteria, or fungi.
- Vectors for cloning genes exist in plants and animals.
- Agrobacterium tumefaciens, a plant pathogenic bacterium, produces plant tumors in almost all dicotyledonous plants but not in cereals.
- Tumor formation is induced by its plasmid called Ti plasmid (Tumor-inducing plasmid).
- This bacterium naturally transfers part of the Ti plasmid into the host plant DNA without any human intervention.
- The part of the Ti plasmid responsible for tumors is called T-DNA.
- Inside the host plant cell, T-DNA separates from the Ti plasmid and integrates into the host plant DNA to cause tumors.
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