Pathology Techniques Course Quiz

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Which department does Doctor Sara Fraticelli belong to?

Department of Molecular Medicine (correct)

Unit of Pathology

Department of Immunohistochemistry

Department of Electron Microscopy

What is the structure of the course?

Endocrinology, Neurology, Cardiology

Introduction, Analysis of Tumours, Recommended Textbook

Immunohistochemistry, PCR, RNA Analysis (correct)

Chemistry, Biology, Physics

How many points are awarded for each correct answer in the exam?

4 points

3 points

2 points

1 point (correct)

What is the minimum number of points required to pass the exam?

18 points Signup and view all the answers

What are the PDF handouts given before/after each lesson?

Free on-line at Springer website as PDF Signup and view all the answers

When was Pathology born?

When physicians began to search for correlations between morphologic and clinical changes Signup and view all the answers

Who is considered as the greatest medical figure of his time and maybe of all times?

Galen Signup and view all the answers

Which Roman writer wrote De Re Medica in eight volumes?

Cornelius Celsus Signup and view all the answers

Who left remarkably clear descriptions of many pathological features such as wound inflammation, tumours, haemorrhoids, malaria, and tuberculosis?

Hippocrates of Cos Signup and view all the answers

Who pursued anatomy as a science and constantly tried to correlate structure with disease?

Herophilus of Chalcedon Signup and view all the answers

Who is believed to be among the first to pursue anatomy as a science and constantly tried to correlate structure with disease?

Herophilus of Chalcedon Signup and view all the answers

Who wrote the classic definition of inflammation: 'Notae vero inflammationis sunt quatuor, rubor et tumor, cum calore et dolore'?

Cornelius Celsus Signup and view all the answers

What is the role of dNTPs in the Polymerase Chain Reaction (PCR)?

To be used by Taq polymerase to build new template molecules Signup and view all the answers

At which temperature does the denaturation step occur in the Polymerase Chain Reaction (PCR)?

~ 95°C Signup and view all the answers

What is the function of the forward and reverse primers in PCR?

To amplify a specific DNA sequence by binding to sense and anti-sense strands of the template Signup and view all the answers

What is the purpose of using a thermal cycler in PCR?

To perform the different temperature cycles required for denaturation, annealing, and elongation steps Signup and view all the answers

What is the base for many laboratory methods involving small amounts of DNA in various fields such as microbiology, genetics, and forensic sciences?

Polymerase Chain Reaction (PCR) Signup and view all the answers

What is the function of MgCl2 in Polymerase Chain Reaction (PCR)?

To act as a catalyst needed by Taq polymerase to work and for specificity Signup and view all the answers

What is the purpose of Sanger sequencing?

Validation of deep sequencing results Signup and view all the answers

What is the main drawback of using intercalating dye-based method in real-time PCR?

Less specific than labelled probe-based technique Signup and view all the answers

Which enzyme is responsible for removing the probe by extending DNA in hydrolysis probe-based method in real-time PCR?

Taq DNA polymerase Signup and view all the answers

What is the primary function of real-time PCR?

Simultaneous amplification and quantification of DNA or RNA Signup and view all the answers

What is the main advantage of Sanger sequencing over Next Generation Sequencing?

Validation of deep sequencing results Signup and view all the answers

What can lead to no amplification in PCR due to Taq polymerase?

PCR inhibitors like ethanol Signup and view all the answers

What is the purpose of fluorescent dye in real-time PCR?

To monitor the reaction using fluorescence Signup and view all the answers

What is the main difference between intercalating dye-based method and hydrolysis probe-based method in real-time PCR?

Fluorescence emission mechanism Signup and view all the answers

Which step does Sanger sequencing substitute for?

'Melt curve analysis Signup and view all the answers

What does hydrolysis probe-based method involve in real-time PCR?

Hybridisation of probe and target separates reporter and quencher Signup and view all the answers