Pathology Techniques Course Quiz
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Pathology Techniques Course Quiz

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@OrganizedIrony

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Questions and Answers

Which department does Doctor Sara Fraticelli belong to?

  • Department of Molecular Medicine (correct)
  • Unit of Pathology
  • Department of Immunohistochemistry
  • Department of Electron Microscopy

    • What is the structure of the course?

  • Endocrinology, Neurology, Cardiology
  • Introduction, Analysis of Tumours, Recommended Textbook
  • Immunohistochemistry, PCR, RNA Analysis (correct)
  • Chemistry, Biology, Physics

    • How many points are awarded for each correct answer in the exam?

  • 4 points
  • 3 points
  • 2 points
  • 1 point (correct)

    • What is the minimum number of points required to pass the exam?

    <p>18 points</p> Signup and view all the answers

    What are the PDF handouts given before/after each lesson?

    <p>Free on-line at Springer website as PDF</p> Signup and view all the answers

    When was Pathology born?

    <p>When physicians began to search for correlations between morphologic and clinical changes</p> Signup and view all the answers

    Who is considered as the greatest medical figure of his time and maybe of all times?

    <p>Galen</p> Signup and view all the answers

    Which Roman writer wrote De Re Medica in eight volumes?

    <p>Cornelius Celsus</p> Signup and view all the answers

    Who left remarkably clear descriptions of many pathological features such as wound inflammation, tumours, haemorrhoids, malaria, and tuberculosis?

    <p>Hippocrates of Cos</p> Signup and view all the answers

    Who pursued anatomy as a science and constantly tried to correlate structure with disease?

    <p>Herophilus of Chalcedon</p> Signup and view all the answers

    Who is believed to be among the first to pursue anatomy as a science and constantly tried to correlate structure with disease?

    <p>Herophilus of Chalcedon</p> Signup and view all the answers

    Who wrote the classic definition of inflammation: 'Notae vero inflammationis sunt quatuor, rubor et tumor, cum calore et dolore'?

    <p>Cornelius Celsus</p> Signup and view all the answers

    What is the role of dNTPs in the Polymerase Chain Reaction (PCR)?

    <p>To be used by Taq polymerase to build new template molecules</p> Signup and view all the answers

    At which temperature does the denaturation step occur in the Polymerase Chain Reaction (PCR)?

    <p>~ 95°C</p> Signup and view all the answers

    What is the function of the forward and reverse primers in PCR?

    <p>To amplify a specific DNA sequence by binding to sense and anti-sense strands of the template</p> Signup and view all the answers

    What is the purpose of using a thermal cycler in PCR?

    <p>To perform the different temperature cycles required for denaturation, annealing, and elongation steps</p> Signup and view all the answers

    What is the base for many laboratory methods involving small amounts of DNA in various fields such as microbiology, genetics, and forensic sciences?

    <p>Polymerase Chain Reaction (PCR)</p> Signup and view all the answers

    What is the function of MgCl2 in Polymerase Chain Reaction (PCR)?

    <p>To act as a catalyst needed by Taq polymerase to work and for specificity</p> Signup and view all the answers

    What is the purpose of Sanger sequencing?

    <p>Validation of deep sequencing results</p> Signup and view all the answers

    What is the main drawback of using intercalating dye-based method in real-time PCR?

    <p>Less specific than labelled probe-based technique</p> Signup and view all the answers

    Which enzyme is responsible for removing the probe by extending DNA in hydrolysis probe-based method in real-time PCR?

    <p>Taq DNA polymerase</p> Signup and view all the answers

    What is the primary function of real-time PCR?

    <p>Simultaneous amplification and quantification of DNA or RNA</p> Signup and view all the answers

    What is the main advantage of Sanger sequencing over Next Generation Sequencing?

    <p>Validation of deep sequencing results</p> Signup and view all the answers

    What can lead to no amplification in PCR due to Taq polymerase?

    <p>PCR inhibitors like ethanol</p> Signup and view all the answers

    What is the purpose of fluorescent dye in real-time PCR?

    <p>To monitor the reaction using fluorescence</p> Signup and view all the answers

    What is the main difference between intercalating dye-based method and hydrolysis probe-based method in real-time PCR?

    <p>Fluorescence emission mechanism</p> Signup and view all the answers

    Which step does Sanger sequencing substitute for?

    <p>'Melt curve analysis</p> Signup and view all the answers

    What does hydrolysis probe-based method involve in real-time PCR?

    <p>Hybridisation of probe and target separates reporter and quencher</p> Signup and view all the answers

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