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Questions and Answers
Which technique is used in patch clamping to control the voltage across the cell membrane?
What type of cells is the patch clamp technique especially useful in studying?
Who developed the patch clamp technique in the late 1970s and early 1980s?
What is recorded in the current clamp technique used in patch clamping?
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Apart from excitable cells, what else can the patch clamp technique be applied to study?
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What is the primary function of a clamper circuit?
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What components are typically found in a simple clamper circuit?
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What does a clamper circuit do to the waveform of the applied signal?
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What type of energy storage elements are commonly used in clamper circuits?
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What condition must be satisfied to maintain the time period of the waveform in a clamper circuit?
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Study Notes
Patch Clamp Technique
- Voltage clamp technique is used to control the voltage across the cell membrane in patch clamping.
- Patch clamp technique is especially useful in studying excitable cells, such as neurons.
Development of Patch Clamp Technique
- The patch clamp technique was developed by Erwin Neher and Bert Sakmann in the late 1970s and early 1980s.
Current Clamp Technique
- In the current clamp technique, the current flowing through the cell membrane is recorded.
Applications of Patch Clamp Technique
- Apart from excitable cells, the patch clamp technique can be applied to study endocrine cells, muscle cells, and epithelial cells.
Clamper Circuit
- The primary function of a clamper circuit is to shift the direct current (DC) level of a signal to a desired value.
- A simple clamper circuit typically consists of a diode, a capacitor, and a resistor.
- A clamper circuit shifts the DC level of the applied signal, without changing its waveform.
Energy Storage Elements
- Capacitors are commonly used as energy storage elements in clamper circuits.
Maintaining Waveform Time Period
- The time constant of the capacitor and resistor must be much smaller than the time period of the waveform to maintain the time period in a clamper circuit.
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Description
Test your knowledge of the patch clamp technique with this quiz, which covers its use in studying ionic currents in excitable cells like neurons and cardiomyocytes, as well as its application in the study of bacterial ion channels.
