Parasitology 3 - Diagnostics
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Questions and Answers

Which staining method is most frequently used for detecting parasites in blood?

  • Giemsa stain (correct)
  • Wright's stain
  • Gram stain
  • Hemotoxylin stain
  • What is a common clinical sign associated with parasitic infections?

  • Frequent urination
  • Anemia (correct)
  • Increased appetite
  • Hyperactivity
  • Which of the following parasites can be detected in peripheral blood?

  • Giardia
  • Ascaris lumbricoides
  • Babesia (correct)
  • Toxocara canis
  • What sample is required for the quantitative microfilariae count?

    <p>1ml EDTA blood (D)</p> Signup and view all the answers

    Which technique is NOT recommended for fecal examination?

    <p>Fecal loops (C)</p> Signup and view all the answers

    What is the role of the Modified Knott’s test?

    <p>To detect heartworm microfilariae (D)</p> Signup and view all the answers

    Which of the following parasites is transmitted via ticks?

    <p>Dirofilaria immitis (B)</p> Signup and view all the answers

    Which clinical sign indicates a parasitic infection in a patient?

    <p>Coughing (D)</p> Signup and view all the answers

    What are the two main categories of parasitology diagnostics?

    <p>Tests based on morphological identification and tests that do not rely on morphology. (A)</p> Signup and view all the answers

    Which of the following is an example of a test that is based on visual morphological identification?

    <p>Geima staining. (C)</p> Signup and view all the answers

    What is considered a good sample handling practice for parasitological samples?

    <p>Refrigerate or use a preservation solution if fresh samples are not available. (C)</p> Signup and view all the answers

    What is the advantage of using fresh samples in parasitology diagnostics?

    <p>They provide more accurate diagnostic results. (C)</p> Signup and view all the answers

    What is the primary purpose of using quantitative tests in parasitology?

    <p>To calculate the number of parasite eggs. (A)</p> Signup and view all the answers

    Which of the following should be considered when collecting parasitological samples to minimize contamination risks?

    <p>Consider opportunistic collections. (B)</p> Signup and view all the answers

    Which factor is critical for the success of parasitological diagnostic tests?

    <p>Having a good quality sample. (B)</p> Signup and view all the answers

    When might it be necessary to send samples out to a diagnostic laboratory?

    <p>When in-house testing equipment is inadequate or unavailable. (C)</p> Signup and view all the answers

    What is the primary purpose of a direct fecal mount?

    <p>To detect and identify protozoan trophozoites, cysts, and oocysts in fresh feces (C)</p> Signup and view all the answers

    What is the significance of centrifugation in the flotation procedure?

    <p>It increases the recovery of eggs by bringing them to the surface (D)</p> Signup and view all the answers

    Which of the following is a limitation of passive flotation techniques?

    <p>It may miss many of the heavier eggs due to lack of energy input (A)</p> Signup and view all the answers

    Which specific gravity range is appropriate for a sugar solution used in the flotation of eunipworms?

    <p>1.25 - 1.8 (B)</p> Signup and view all the answers

    What types of eggs are primarily recovered using sedimentation techniques?

    <p>Trematode eggs that do not float (A)</p> Signup and view all the answers

    Which of the following methods is least effective for recovering all types of eggs from fecal samples?

    <p>Passive flotation (C)</p> Signup and view all the answers

    Which organism's eggs are specifically mentioned as being recovered using sedimentation techniques?

    <p>Fasciola hepatica (B)</p> Signup and view all the answers

    What challenge is associated with flotation methods in terms of egg recovery?

    <p>Some eggs are heavier and do not float (C)</p> Signup and view all the answers

    What is the primary purpose of the Baermann technique?

    <p>To recover live nematode larvae from fresh feces (A)</p> Signup and view all the answers

    Which of the following is NOT a reason for quantifying fecal diagnostics?

    <p>Assessing overall health of a herd (C)</p> Signup and view all the answers

    What is the minimum egg per gram (EPG) detection limit for the 2g/18mL miniFLOTAC method?

    <p>5 EPG (C)</p> Signup and view all the answers

    Which parasitological test is specifically mentioned for recovering ova from urine?

    <p>Dioctophyme renale test (A)</p> Signup and view all the answers

    What is a key factor regarding the variability of parasite tolerance among animals in a herd?

    <p>20-30% of animals hold 80% of the parasites. (C)</p> Signup and view all the answers

    Which method can be used for detecting genetic markers of parasites?

    <p>PCR (C)</p> Signup and view all the answers

    In the context of fecal diagnostics, what does EPG stand for?

    <p>Eggs Per Gram (A)</p> Signup and view all the answers

    Which non-morphological diagnostic method uses blood or feces to identify parasites?

    <p>Serology (D)</p> Signup and view all the answers

    Flashcards

    Parasitology Diagnostics Categories

    Techniques used to identify parasites, categorized as visual identification and non-visual tests.

    Visual Parasite Identification

    Diagnosing parasites by physically examining samples like blood, feces, urine, or tissue sections using stains/procedures like Giemsa or McMaster.

    Non-visual Parasite Identification

    Techniques like fecal antigen tests, PCR, or serology that don't directly visualize the parasite.

    Qualitative vs. Quantitative Tests

    Qualitative tests detect the presence or absence of a parasite, while quantitative tests measure the number of parasites present.

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    Fecal Examination (Fec)

    A diagnostic test used to identify parasites in fecal matter.

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    Sample Handling (Parasitology)

    Proper methods for collecting, preserving, and submitting parasite specimens.

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    Biohazard Potential (Parasitology)

    Recognition of the risk of infection from parasitological samples.

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    Fresh Sample Importance

    Fresh samples are ideal for parasite diagnostics but freezing/preservatives are necessary if fresh aren't possible.

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    Parasite Detection in Blood

    Methods to find parasitic protozoa (like Trypanosoma and Babesia) and nematode larvae (microfilariae) in blood samples.

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    Blood Smear Stain

    Giemsa stain is commonly used to visually detect parasites in blood samples.

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    Clinical Signs of Parasitism

    Symptoms like anemia, fever, lethargy, or exposure to ectoparasites can indicate blood parasites.

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    Fecal Parasite Detection

    Methods to identify parasites in fecal samples to diagnose intestinal infections.

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    Fecal Examination Techniques

    Direct mounts, flotation, sedimentation, Baermann, and McMaster techniques help find parasites in feces.

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    Fecal Loop Contraindicated

    Fecal loops should not be used for parasite detection.

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    Cytauxzoon felis Transmission

    Cytauxzoon felis, a parasite, is transmitted by ticks.

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    Heartworm Identification

    Differentiating between Dirofilaria immitis and Acanthocheilonema reconditum in blood samples.

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    Direct Fecal Mount

    A method used to identify protozoa, cysts, oocysts, and some larvae in fresh feces.

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    Flotation (with centrifugation)

    Separates eggs/cysts by density; a high-specific gravity solution sinks heavier materials.

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    Flotation solution

    A solution with high specific gravity for flotation.

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    Passive Flotation

    Eggs/cysts float by specific gravity, no special procedures.

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    Sedimentation

    Separating eggs of trematodes (flukes) by letting solids settle.

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    Baermann Technique

    Used to recover trematode eggs by allowing them to be washed through a liquid.

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    Specific Gravity (SG)

    Density of a substance compared to water. Used in flotation solutions.

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    Protozoa Trophozoites, Cysts & Oocysts

    Single-celled organisms, stages of their lifecycle, and other pathogens identified in fecal analysis.

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    Quantitative Fecal Diagnostics

    Methods to estimate the level of parasite infection, not just the presence.

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    McMasters Technique

    A fecal flotation diagnostic method, ideal for horses and ruminants.

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    Dilution Egg Counts

    More precise parasite level measurement when egg per gram (EPG) counts are high.

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    Selective Deworming

    Treating only animals that have high parasite counts.

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    Determining Treatment Efficacy

    Assessing the success of parasite control measures.

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    Skin Scrape

    A method to find small ectoparasites (e.g., mites).

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    Non-morphological Diagnostics

    Parasite identification methods that don't rely on looking at the parasite's shape.

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    Study Notes

    Parasitology 3 - Diagnostics

    • Students will learn the various techniques used to diagnose parasites.
    • Students will determine the appropriate diagnostic test, deciding if it should be performed in-house or sent to a lab.
    • Students will understand the crucial role of proper sample handling in diagnostics (collection, submission, and preservation).
    • Students will recognize the biohazard potential of parasitological samples.

    Useful Sites

    Parasitology Diagnostics Categories

    • Tests based on visual (morphological) identification of parasite stages:
      • Blood, feces, urine, tissue sections
      • Examples: Giemsa staining, McMaster FEC, sedimentation, histopathology
    • Tests not based on morphology:
      • Examples: Fecal antigen, PCR, serology

    Qualitative vs Quantitative Testing

    • Qualitative: presence/absence (yes/no), frequently used with small animals
    • Quantitative: number of parasites (count eggs and calculate), frequently used with large animals/ruminants

    Good Sample Collection Practices

    • Fresh samples are ideal.
    • If fresh samples are not possible, refrigeration or preservation solutions (e.g., formalin, ethanol) are acceptable.
    • Avoid risky collection practices. Take proper precautions to prevent contamination.
    • Collect adequate samples (at least 1 gram of feces for flotation) for accurate results.
    • Appropriate collection methods (e.g. temperature probe for Giardia, last drop of blood in the syringe for microfilariae) are essential.
    • Consider opportunistic collections.
    • Consult a parasitologist when in doubt.

    Looking for Parasites in Blood

    • Identify parasitic protozoa (e.g., Trypanosoma, Babesia).
    • Recognize the first-stage larvae (microfilariae) of tissue-dwelling nematodes (e.g., D. immitis).
    • Giemsa staining is frequently used.
    • Clinical signs (anemia, fever, lethargy) along with a known exposure to ectoparasites are significant indicators.
    • Heartworm testing & microfilaria identification are important.
    • Modified Knott's test or quantitative microfilariae count are necessary. 1 mL EDTA blood, 2% formalin, methylene blue are required for testing

    Looking for Parasites in Feces

    • Clinical signs (diarrhea, weight loss, ill-thrift, coughing) suggest parasite presence.
    • Multiple testing options are available:
      • Direct Mount
      • Flotation with Centrifugation
      • Sedimentation
      • Baermann technique
      • McMaster Fecal Egg Count

    Direct Fecal Mount

    • Used to detect and identify protozoan trophozoites, cysts, and oocysts, and some larvae.
    • Use fresh feces with saline and Lugol's solutions.
    • Observe for motility.

    Flotation with Centrifugation

    • Improves efficiency by concentrating the eggs/cysts to the surface.
    • A solution of high specific gravity is needed.
    • Examine the surface of the fecal suspension microscopically.

    Sedimentation & Baermann Technique

    • Sedimentation: used to recover trematode eggs (do not float using standard flotation).
    • Baermann technique: used to recover live nematode larvae from fresh feces and sample analysis of lung worms, etc.

    Quantitative Fecal Diagnostics (McMaster)

    • Dilution egg counts offer more accurate results when egg per gram (EPG) counts are high.
    • Useful for estimating strongyle infections in ruminants and horses.
    • Different protocols, chambers, have varying minimum detection limits.

    Why Quantify Fecal Parasites?

    • Identify high shedders for contamination risk.
    • Enable selective deworming.
    • Determine treatment efficacy, particularly in herds where resistance can vary among animals.

    Urine Samples / Tests

    • Ova are frequently seen in urine sediment.
    • Dioctophyme renale (giant kidney worm) identification.
    • Similar process for BAL/TTW recovery (ova, larvae)

    Non-Morphological Diagnostics

    • Antigen: detecting parasite antigens in blood/serum or feces (coproantigen).
    • Serology: detecting antibodies produced by host to parasites.
    • PCR: detecting genetic markers to identify parasite stages.

    Ectoparasite Diagnostics

    • Skin Scrape: for small ectoparasites, especially mites.
    • Swab & Slide: for mites.
    • Skin and Hair Coat Examination: visual inspection.
    • Flea Comb: used to find fleas.
    • Vacuum: used to collect ectoparasites

    Egg/Oocyst Comparison (Canine, Feline, Equine, Ruminant)

    • Images display various egg/oocyst sizes and shapes for different species of parasites.

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    Parasitology Diagnostics PDF

    Description

    This quiz focuses on the diagnostic techniques used in parasitology. Students will explore the appropriate tests for diagnosing parasites and understand the importance of sample handling. Additionally, the potential biohazard risks associated with parasitological samples will be discussed.

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