Parasitic Infections Diagnostic Techniques

Play an AI-generated podcast conversation about this lesson

Why is it essential to deliver the stool sample to the laboratory immediately or within an hour?

To reduce the cost of the laboratory test
To prevent contamination of the sample
To prevent the disintegration of some development stages of parasites (correct)
To analyze the sample quickly

What is the purpose of adding Eosin in the preparation of the direct faecal wet mount?

To preserve the sample for later analysis
To study the morphology of the parasite and identify the parasite species
To detect the presence of any motile live development stages of the parasites
To screen the fresh sample for presence of protozoa only (correct)

Why is it important to prepare the wet mount from the stool sample as soon as possible?

To prevent the faecal sample from drying up and resulting in the death of parasites (correct)
To allow the parasites to develop further
To analyze the sample quickly
To allow the sample to settle

What is the purpose of adding Lugol's iodine in the preparation of the direct faecal wet mount?

To study the morphology of the parasite and identify the parasite species (D) Signup and view all the answers

What is the purpose of adding normal saline in the preparation of the direct faecal wet mount?

To detect the presence of any motile live development stages of the parasites (A) Signup and view all the answers

What is the primary concern while collecting a stool sample?

To collect a sample that is free from urine and water (A) Signup and view all the answers

What is the purpose of adding eosin to the faeces in the first step of the direct faecal wet mount?

To enhance the visibility of the protozoa under the microscope (C) Signup and view all the answers

What is the limitation of the direct faecal wet mount?

It is not suitable for long-term storage (D) Signup and view all the answers

How many wet mounts are prepared in the procedure described?

Three (A) Signup and view all the answers

What is the purpose of preparing a trichrome-stained permanent mount?

To preserve the faecal sample for long-term storage and record (A) Signup and view all the answers

What is the name of the stain used in the trichrome-stained permanent mount?

Trichrome (A) Signup and view all the answers

What is the reason for treating the faecal smear with chemical reagents before staining?

To fix the protozoa to the slide (B) Signup and view all the answers

What can be found in the stools of an infected person?

A whole or part of an adult worm (B) Signup and view all the answers

What type of blood film is used for estimating the number of parasites in the patient's blood?

Thick blood film (A) Signup and view all the answers

What is the purpose of preparing a blood film as soon as possible?

To prevent the blood sample from clotting (A) Signup and view all the answers

What is the purpose of a thin blood film?

To examine the morphology of the parasite (B) Signup and view all the answers

What type of parasite is Entamoeba histolytica?

Intestinal amoeba (D) Signup and view all the answers

What is the purpose of a blood film?

To assess the severity of infection and identify the species (C) Signup and view all the answers

What is the natural host of Toxoplasma gondii?

Cats and the related feline species (A) Signup and view all the answers

Why is laboratory diagnosis of toxoplasmosis dependent on serology?

Because the parasite can cause asymptomatic latent infection (A) Signup and view all the answers

How is Toxoplasma gondii transmitted?

Through the oocyst passed in the cat faeces (D) Signup and view all the answers

What is the common symptom of amoebic liver abscess?

Pain at the lower right quadrant (B) Signup and view all the answers

Why is direct detection of Entamoeba histolytica not preferred?

Because it requires invasive technique (C) Signup and view all the answers

What is the significance of oocyst in the transmission of toxoplasmosis?

It is a developmental form of the parasite (C) Signup and view all the answers

What is the ideal amount of blood to be placed onto a clean slide for preparing a thin blood film?

3-5 drops (C) Signup and view all the answers

What is the purpose of staining a blood film?

To reveal the detail morphology of the parasite and blood cells (A) Signup and view all the answers

Why are some parasitic infections diagnosed through blood tests?

Because parasites are in the tissues and not obtainable in the faeces or blood (B) Signup and view all the answers

What type of parasite is responsible for elephantiasis?

Nematode (B) Signup and view all the answers

What is the purpose of microscopic examination of a stained blood film?

To examine the morphology of the parasite and blood cells (D) Signup and view all the answers

What is the principle of serology?

Detecting circulating antigens or antibodies in the blood (B) Signup and view all the answers