Parasitic Infections Diagnostic Techniques
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Questions and Answers

Why is it essential to deliver the stool sample to the laboratory immediately or within an hour?

  • To reduce the cost of the laboratory test
  • To prevent contamination of the sample
  • To prevent the disintegration of some development stages of parasites (correct)
  • To analyze the sample quickly
  • What is the purpose of adding Eosin in the preparation of the direct faecal wet mount?

  • To preserve the sample for later analysis
  • To study the morphology of the parasite and identify the parasite species
  • To detect the presence of any motile live development stages of the parasites
  • To screen the fresh sample for presence of protozoa only (correct)
  • Why is it important to prepare the wet mount from the stool sample as soon as possible?

  • To prevent the faecal sample from drying up and resulting in the death of parasites (correct)
  • To allow the parasites to develop further
  • To analyze the sample quickly
  • To allow the sample to settle
  • What is the purpose of adding Lugol's iodine in the preparation of the direct faecal wet mount?

    <p>To study the morphology of the parasite and identify the parasite species</p> Signup and view all the answers

    What is the purpose of adding normal saline in the preparation of the direct faecal wet mount?

    <p>To detect the presence of any motile live development stages of the parasites</p> Signup and view all the answers

    What is the primary concern while collecting a stool sample?

    <p>To collect a sample that is free from urine and water</p> Signup and view all the answers

    What is the purpose of adding eosin to the faeces in the first step of the direct faecal wet mount?

    <p>To enhance the visibility of the protozoa under the microscope</p> Signup and view all the answers

    What is the limitation of the direct faecal wet mount?

    <p>It is not suitable for long-term storage</p> Signup and view all the answers

    How many wet mounts are prepared in the procedure described?

    <p>Three</p> Signup and view all the answers

    What is the purpose of preparing a trichrome-stained permanent mount?

    <p>To preserve the faecal sample for long-term storage and record</p> Signup and view all the answers

    What is the name of the stain used in the trichrome-stained permanent mount?

    <p>Trichrome</p> Signup and view all the answers

    What is the reason for treating the faecal smear with chemical reagents before staining?

    <p>To fix the protozoa to the slide</p> Signup and view all the answers

    What can be found in the stools of an infected person?

    <p>A whole or part of an adult worm</p> Signup and view all the answers

    What type of blood film is used for estimating the number of parasites in the patient's blood?

    <p>Thick blood film</p> Signup and view all the answers

    What is the purpose of preparing a blood film as soon as possible?

    <p>To prevent the blood sample from clotting</p> Signup and view all the answers

    What is the purpose of a thin blood film?

    <p>To examine the morphology of the parasite</p> Signup and view all the answers

    What type of parasite is Entamoeba histolytica?

    <p>Intestinal amoeba</p> Signup and view all the answers

    What is the purpose of a blood film?

    <p>To assess the severity of infection and identify the species</p> Signup and view all the answers

    What is the natural host of Toxoplasma gondii?

    <p>Cats and the related feline species</p> Signup and view all the answers

    Why is laboratory diagnosis of toxoplasmosis dependent on serology?

    <p>Because the parasite can cause asymptomatic latent infection</p> Signup and view all the answers

    How is Toxoplasma gondii transmitted?

    <p>Through the oocyst passed in the cat faeces</p> Signup and view all the answers

    What is the common symptom of amoebic liver abscess?

    <p>Pain at the lower right quadrant</p> Signup and view all the answers

    Why is direct detection of Entamoeba histolytica not preferred?

    <p>Because it requires invasive technique</p> Signup and view all the answers

    What is the significance of oocyst in the transmission of toxoplasmosis?

    <p>It is a developmental form of the parasite</p> Signup and view all the answers

    What is the ideal amount of blood to be placed onto a clean slide for preparing a thin blood film?

    <p>3-5 drops</p> Signup and view all the answers

    What is the purpose of staining a blood film?

    <p>To reveal the detail morphology of the parasite and blood cells</p> Signup and view all the answers

    Why are some parasitic infections diagnosed through blood tests?

    <p>Because parasites are in the tissues and not obtainable in the faeces or blood</p> Signup and view all the answers

    What type of parasite is responsible for elephantiasis?

    <p>Nematode</p> Signup and view all the answers

    What is the purpose of microscopic examination of a stained blood film?

    <p>To examine the morphology of the parasite and blood cells</p> Signup and view all the answers

    What is the principle of serology?

    <p>Detecting circulating antigens or antibodies in the blood</p> Signup and view all the answers

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