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Questions and Answers
Explain the sex determination system in papaya and the preferred sex for fruit production.
Explain the sex determination system in papaya and the preferred sex for fruit production.
Papaya is a trioecious species with 3 sexes: H, M, F. Hermaphrodite (H) is preferred for fruit production due to more elongated fruits and smaller fruit cavities.
Describe the molecular markers used for sex determination in papaya and their target sequences.
Describe the molecular markers used for sex determination in papaya and their target sequences.
Molecular markers used for sex determination in papaya include SCAR primer pair markers targeting the Sex1 gene (T1) and the W11 gene. The SCAR markers amplify specific fragments present in different sexes of papaya.
What is the DNA isolation method employed in the study and what reagents are used in the extraction buffer?
What is the DNA isolation method employed in the study and what reagents are used in the extraction buffer?
The DNA isolation method used is CTAB extraction. Reagents in the extraction buffer include Tris-HCl, NaCl, EDTA, CTAB, PVP, β-mercaptoethanol, absolute isopropanol, 70% ethanol solution, and Chloroform:Isoamyl alcohol (24:1).
List the meat products analyzed in the species identification study and the methods used for meat detection.
List the meat products analyzed in the species identification study and the methods used for meat detection.
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Explain the type of DNA targeted and the method used for rapid lysis in the species identification of meat products.
Explain the type of DNA targeted and the method used for rapid lysis in the species identification of meat products.
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Study Notes
Sex Determination in Papaya
- Papaya exhibits a unique sex determination system involving three primary sex types: male, female, and hermaphrodite.
- Hermaphrodite papayas are preferred for commercial fruit production due to their ability to produce fruit without the need for pollination.
Molecular Markers for Sex Determination
- Specific molecular markers are employed for identifying papaya sex, focusing on the Y chromosome.
- Target sequences for these markers include the sex-determining gene and associated markers linked to male and hermaphrodite traits.
DNA Isolation Method
- The study utilizes a modified cetyltrimethylammonium bromide (CTAB) method for DNA isolation from papaya tissues.
- The extraction buffer contains reagents such as CTAB, sodium chloride, EDTA, and Tris-HCl, optimizing the yield and purity of DNA.
Meat Products and Detection Methods
- Various meat products analyzed in the species identification study include beef, pork, chicken, and lamb.
- Detection methods employed include polymerase chain reaction (PCR) for amplification and species-specific primers for accurate identification.
Target DNA and Rapid Lysis Method
- The DNA targeted in meat identification is mitochondrial DNA due to its high copy number and species-specific sequences.
- A rapid lysis method involves using a proteinase K treatment to effectively break down cellular components, enhancing DNA accessibility for analysis.
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Description
Test your knowledge about sex determination in papaya, including the molecular markers and genes involved in identifying the different sexes of papaya plants.
