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Order of Draw in Phlebotomy
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Order of Draw in Phlebotomy

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Questions and Answers

What is the primary reason for collecting blood culture tubes first in the order of draw?

  • To ensure separation of serum from clot
  • To prevent contamination with additives from other tubes (correct)
  • To prevent platelet activation
  • To prevent activation of coagulation factors
  • Why is it essential to use a new needle for each tube?

  • To prevent cross-contamination of blood samples (correct)
  • To follow proper procedures for tube inversion and mixing
  • To handle tubes gently and prevent hemolysis
  • To ensure the correct needle size and type for the patient's vein
  • What is the purpose of collecting serum tubes in the order of draw?

  • To prevent contamination with additives from other tubes
  • To ensure separation of serum from clot (correct)
  • To prevent platelet activation
  • To prevent interference with coagulation tests
  • Why is it important to handle tubes gently during the order of draw?

    <p>To prevent hemolysis</p> Signup and view all the answers

    What is the purpose of collecting EDTA tubes in the order of draw?

    <p>To prevent platelet activation</p> Signup and view all the answers

    What is the final step in the standard order of draw?

    <p>Collecting other tubes according to specific requirements</p> Signup and view all the answers

    What is the primary purpose of using red top tubes?

    <p>For serum chemistry tests</p> Signup and view all the answers

    What concentration of potassium oxalate is used in grey top tubes?

    <p>1.5-2.2 mg/mL</p> Signup and view all the answers

    Which of the following tubes contains sodium citrate?

    <p>Light blue top tubes</p> Signup and view all the answers

    What is the concentration of heparin used in green top tubes?

    <p>15-30 units/mL</p> Signup and view all the answers

    What is the purpose of using lavender top tubes?

    <p>For hematology tests</p> Signup and view all the answers

    What is the concentration of EDTA used in lavender top tubes?

    <p>1.5-2.2 mg/mL</p> Signup and view all the answers

    Study Notes

    Order of Draw

    The order of draw is a standardized procedure for collecting blood samples from a patient to minimize contamination and ensure accurate test results.

    Importance of Order of Draw

    • Prevents cross-contamination of blood samples
    • Reduces the risk of false test results
    • Ensures accurate diagnosis and treatment

    Standard Order of Draw

    1. Blood Culture: Collect blood culture tubes first to prevent contamination with additives from other tubes.
    2. Sodium Citrate (Coagulation) Tubes: Collect citrate tubes next to prevent activation of coagulation factors.
    3. Serum Tubes (Red Top or Gold Top): Collect serum tubes to ensure separation of serum from clot.
    4. Heparin Tubes (Green Top): Collect heparin tubes to prevent interference with coagulation tests.
    5. EDTA Tubes (Lavender Top): Collect EDTA tubes to prevent platelet activation.
    6. Fluoride Tubes (Gray Top): Collect fluoride tubes to prevent glycolysis.
    7. Other Tubes (e.g., Oxalate, Acid-Citrate-Dextrose): Collect other tubes according to specific requirements.

    Key Considerations

    • Always use a new needle for each tube to prevent cross-contamination.
    • Use the correct needle size and type for the patient's vein size and type.
    • Ensure the patient's identification and tube labels match.
    • Handle tubes gently to prevent hemolysis.
    • Follow proper procedures for tube inversion and mixing.

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    Description

    Learn the standardized procedure for collecting blood samples from a patient to minimize contamination and ensure accurate test results. Understand the importance of the order of draw and the key considerations to prevent cross-contamination and ensure accurate diagnosis.

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