Optical Biosensors: Principles and Applications
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Questions and Answers

What is the primary method of detecting and quantifying biological compounds?

  • Electrophoresis
  • Chromatography
  • Fluorescence activity (correct)
  • Spectrophotometry

What is the effect of environmental changes on most organic fluorophores?

  • They are highly resistant to environmental changes
  • They are completely unaffected by environmental changes
  • They are slightly affected by environmental changes
  • Their fluorescent properties are sensitive to environmental changes (correct)

What is the advantage of fluorescence-based detection methods?

  • They have low sensitivity to environmental changes
  • They are highly specific to a single compound
  • They require complex instrumentation
  • They can detect multiple compounds within a single device (correct)

What is the relationship between fluorescence intensity and excitation light intensity?

<p>Fluorescence intensity is directly proportional to excitation light intensity (A)</p> Signup and view all the answers

What is the term that describes the efficiency of a fluorophore in emitting light?

<p>Quantum yield (A)</p> Signup and view all the answers

What is the primary function of a calorimeter?

<p>To measure the energy changes in the form of heat (A)</p> Signup and view all the answers

In biophysics, what is calorimetry typically used to measure?

<p>The amount of energy absorbed or released in biochemical reactions, conformational transitions, or ligand binding (A)</p> Signup and view all the answers

What is an example of a process that can be measured using calorimetry?

<p>The unwinding of a piece of DNA helix (A)</p> Signup and view all the answers

What is the purpose of measuring the binding strength of various drugs to a particular protein using calorimetry?

<p>To identify the most effective drug for a particular task (A)</p> Signup and view all the answers

What is the primary application of calorimetry in biophysics?

<p>To measure the energy changes in biochemical reactions, conformational transitions, and ligand binding (B)</p> Signup and view all the answers

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