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Optical Biosensors: Principles and Applications

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Optical Biosensors: Principles and Applications

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Questions and Answers

What is the primary method of detecting and quantifying biological compounds?

Electrophoresis

Chromatography

Fluorescence activity (correct)

Spectrophotometry

What is the effect of environmental changes on most organic fluorophores?

They are highly resistant to environmental changes

They are completely unaffected by environmental changes

They are slightly affected by environmental changes

Their fluorescent properties are sensitive to environmental changes (correct)

What is the advantage of fluorescence-based detection methods?

They have low sensitivity to environmental changes

They are highly specific to a single compound

They require complex instrumentation

They can detect multiple compounds within a single device (correct)

What is the relationship between fluorescence intensity and excitation light intensity?

Fluorescence intensity is directly proportional to excitation light intensity Signup and view all the answers

What is the term that describes the efficiency of a fluorophore in emitting light?

Quantum yield Signup and view all the answers

What is the primary function of a calorimeter?

To measure the energy changes in the form of heat Signup and view all the answers

In biophysics, what is calorimetry typically used to measure?

The amount of energy absorbed or released in biochemical reactions, conformational transitions, or ligand binding Signup and view all the answers

What is an example of a process that can be measured using calorimetry?

The unwinding of a piece of DNA helix Signup and view all the answers

What is the purpose of measuring the binding strength of various drugs to a particular protein using calorimetry?

To identify the most effective drug for a particular task Signup and view all the answers

What is the primary application of calorimetry in biophysics?

To measure the energy changes in biochemical reactions, conformational transitions, and ligand binding Signup and view all the answers