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Questions and Answers
Why do we put iodine solution on the onion peel when observing it under a microscope?
Why do we put iodine solution on the onion peel when observing it under a microscope?
- To prevent the cells from drying out
- To enhance the contrast between different regions of the cells (correct)
- To eliminate the need for focusing under the microscope
- To increase the size of the cells for better observation
What would happen if we tried observing the onion peel under a microscope without putting iodine solution on it?
What would happen if we tried observing the onion peel under a microscope without putting iodine solution on it?
- The color of the cells would change dramatically
- The cells would become transparent
- The cells would appear larger than usual
- It would be difficult to differentiate between different regions of the cells (correct)
Why might some regions of the cells appear darker than others when observing the onion peel with iodine solution?
Why might some regions of the cells appear darker than others when observing the onion peel with iodine solution?
- As a result of excess light exposure under the microscope
- Because of uneven distribution of water in the cells
- Because the cells are dying
- Due to differences in chemical composition of the cell regions (correct)
In addition to iodine solution, what other substances could be used to stain cells when observing them?
In addition to iodine solution, what other substances could be used to stain cells when observing them?
What is the main purpose of staining cells before observation under a microscope?
What is the main purpose of staining cells before observation under a microscope?
What is the purpose of putting iodine solution on the onion peel before observing it under a microscope?
What is the purpose of putting iodine solution on the onion peel before observing it under a microscope?
What would be the likely outcome if one attempted to observe the onion peel without putting iodine solution?
What would be the likely outcome if one attempted to observe the onion peel without putting iodine solution?
Why do different regions of cells in the onion peel get colored differentially when stained?
Why do different regions of cells in the onion peel get colored differentially when stained?
Apart from iodine solution, what other substance could be used to stain cells when observing them under a microscope?
Apart from iodine solution, what other substance could be used to stain cells when observing them under a microscope?
What is the main advantage of staining cells before observation under a microscope?
What is the main advantage of staining cells before observation under a microscope?
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Study Notes
Iodine Solution and Microscopy
- Iodine solution is applied to the onion peel to enhance visibility of cell structures under a microscope
- Without iodine solution, the onion peel's cellular structures would be difficult to distinguish under the microscope due to their transparency
- Iodine solution selectively stains different regions of cells, causing some areas to appear darker than others due to varying levels of starch storage
Alternative Staining Substances
- Besides iodine, other substances can be used to stain cells, such as methylene blue or safranin
Purpose of Staining
- The primary purpose of staining cells is to increase their contrast, making microscopic observation easier and more accurate
- Staining helps to distinguish between different cellular structures and components, enhancing the quality of microscopic observations
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