Podcast
Questions and Answers
What is the primary difference between nutrient broth and nutrient agar?
What is the primary difference between nutrient broth and nutrient agar?
Nutrient broth does not contain agar, while nutrient agar is a solid medium that includes agar.
List two pieces of equipment required for preparing nutrient agar.
List two pieces of equipment required for preparing nutrient agar.
Conical flask and autoclave.
What temperature and pressure conditions are required for autoclaving nutrient agar?
What temperature and pressure conditions are required for autoclaving nutrient agar?
15 lbs pressure and 121°C temperature.
At what temperature should the nutrient agar be cooled before pouring into Petri plates?
At what temperature should the nutrient agar be cooled before pouring into Petri plates?
Signup and view all the answers
Why is it important for the surface of the nutrient agar to be dry when inoculated?
Why is it important for the surface of the nutrient agar to be dry when inoculated?
Signup and view all the answers
Study Notes
Nutrient Agar Preparation
- Nutrient agar is a growth medium used in microbiology.
- It does not contain nutrient broth.
- Preparation requires specific equipment including conical flasks, measuring cylinders, weighing balances, hot plates, stirrers, autoclaves, ovens, and sterile petri dishes.
- Supplies needed include dehydrated media and distilled water.
Procedure
- Mix 28 grams of nutrient agar with 1000 ml of distilled water. Heat the mixture to a boil to completely dissolve the agar.
- Sterilize the solution by autoclaving at 15 pounds per square inch (psi) pressure and 121°C temperature for 15 minutes.
- Cool the solution to a temperature of 45-50°C.
- Pour the cooled solution into sterile petri dishes until the surface is dry.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Description
Test your knowledge on the preparation of nutrient agar, a key growth medium used in microbiology. This quiz covers the equipment, supplies, and step-by-step procedures necessary for creating this essential laboratory material. Explore the important details to ensure successful agar preparation.
