25 Questions
What is the function of bacterial small RNAs?
Regulate gene expression
What is the role of Hfq in bacterial regulatory RNA function?
Aids binding of short, imperfect matching mRNA-sRNA and stabilizes it
What is the function of riboswitches in gene transcripts?
Control mRNA translation in response to changes in metabolite amount
How do riboswitches respond to changes in metabolite concentration?
By forming anti-termination structures
What is the consequence of activated riboswitch structures?
They block further transcription or any translation
How do regulatory RNAs in bacteria affect mRNA translation?
By binding to key regions and blocking translation
What is the role of σS factor in bacterial cells during stationary phase?
Promotes transcription of survival proteins
What is the function of sRNAs in bacteria?
Repress levels of one protein and promote another
What is the role of 6S RNA in growing E. coli cells during stationary phase?
Binds RNA Pol and down-regulates transcription
What is the function of RNA's single stranded properties in regulatory roles?
Facilitate regulatory roles
What is the role of sRNA binding to key regions in bacterial mRNA translation?
Blocks ribosomes and mRNA translation
What is the consequence of riboswitch structures altering ribosome interactions?
Blocks or increases access to ribosome binding site
Regulatory RNAs exploit Watson-Crick base-pairing to other RNAs, DNAs to block binding, and alter RNA structure
True
Riboswitches are built-in metabolite sensors that use RNA sequences to control mRNA translation in response to changes in metabolite amount.
True
Riboswitches are usually found downstream of genes involved in the synthesis of the metabolite that the riboswitch responds to.
False
Riboswitches can bind and respond to approximately 19 recognized metabolites, each with unique structures.
False
Activated riboswitch structures only block further transcription, but do not affect any translation.
False
Riboswitches exhibit protein-like structure diversity.
True
Small antisense RNAs may be transcribed, bind to, and block target mRNAs in bacterial cells.
True
Bacterial small RNAs can regulate gene expression by binding to complementary target mRNAs to form double-stranded RNAs.
True
6S RNA binds RNA Pol and down-regulates transcription in growing E. coli cells during stationary phase.
True
Bacterial mRNA translation can be regulated by sRNA binding to key regions.
True
Regulatory RNAs in bacteria, such as sRNAs, always serve to repress levels of one protein and promote another.
False
Riboswitches can respond to uncharged tRNAs through the aminoacyl tRNA synthetase gene.
True
A gene's transcripts in bacteria can directly sense changes in metabolite concentration.
True
Test your knowledge of nucleic acids biochemistry, regulatory RNAs, and examples of prokaryotic and eukaryotic promoters with this quiz. Review the concepts of eukaryotic promoter sequences, the role of sigma factors and general transcription factors, as well as the process of pre-replicative complex assembly and promoter escape.
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