Nucleic Acids Biochemistry and Promoter Sequences Quiz
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Questions and Answers

What is the function of bacterial small RNAs?

  • Promote protein synthesis
  • Regulate gene expression (correct)
  • Facilitate DNA replication
  • Inhibit RNA polymerase activity

What is the role of Hfq in bacterial regulatory RNA function?

  • Inhibits sRNA-mRNA interaction
  • Promotes ribosome binding to mRNA
  • Catalyzes mRNA degradation
  • Aids binding of short, imperfect matching mRNA-sRNA and stabilizes it (correct)

What is the function of riboswitches in gene transcripts?

  • Control mRNA translation in response to changes in metabolite amount (correct)
  • Inhibit mRNA transcription
  • Facilitate protein folding
  • Promote DNA replication

How do riboswitches respond to changes in metabolite concentration?

<p>By forming anti-termination structures (A)</p> Signup and view all the answers

What is the consequence of activated riboswitch structures?

<p>They block further transcription or any translation (D)</p> Signup and view all the answers

How do regulatory RNAs in bacteria affect mRNA translation?

<p>By binding to key regions and blocking translation (A)</p> Signup and view all the answers

What is the role of σS factor in bacterial cells during stationary phase?

<p>Promotes transcription of survival proteins (C)</p> Signup and view all the answers

What is the function of sRNAs in bacteria?

<p>Repress levels of one protein and promote another (C)</p> Signup and view all the answers

What is the role of 6S RNA in growing E. coli cells during stationary phase?

<p>Binds RNA Pol and down-regulates transcription (A)</p> Signup and view all the answers

What is the function of RNA's single stranded properties in regulatory roles?

<p>Facilitate regulatory roles (B)</p> Signup and view all the answers

What is the role of sRNA binding to key regions in bacterial mRNA translation?

<p>Blocks ribosomes and mRNA translation (B)</p> Signup and view all the answers

What is the consequence of riboswitch structures altering ribosome interactions?

<p>Blocks or increases access to ribosome binding site (A)</p> Signup and view all the answers

Regulatory RNAs exploit Watson-Crick base-pairing to other RNAs, DNAs to block binding, and alter RNA structure

<p>True (A)</p> Signup and view all the answers

Riboswitches are built-in metabolite sensors that use RNA sequences to control mRNA translation in response to changes in metabolite amount.

<p>True (A)</p> Signup and view all the answers

Riboswitches are usually found downstream of genes involved in the synthesis of the metabolite that the riboswitch responds to.

<p>False (B)</p> Signup and view all the answers

Riboswitches can bind and respond to approximately 19 recognized metabolites, each with unique structures.

<p>False (B)</p> Signup and view all the answers

Activated riboswitch structures only block further transcription, but do not affect any translation.

<p>False (B)</p> Signup and view all the answers

Riboswitches exhibit protein-like structure diversity.

<p>True (A)</p> Signup and view all the answers

Small antisense RNAs may be transcribed, bind to, and block target mRNAs in bacterial cells.

<p>True (A)</p> Signup and view all the answers

Bacterial small RNAs can regulate gene expression by binding to complementary target mRNAs to form double-stranded RNAs.

<p>True (A)</p> Signup and view all the answers

6S RNA binds RNA Pol and down-regulates transcription in growing E. coli cells during stationary phase.

<p>True (A)</p> Signup and view all the answers

Bacterial mRNA translation can be regulated by sRNA binding to key regions.

<p>True (A)</p> Signup and view all the answers

Regulatory RNAs in bacteria, such as sRNAs, always serve to repress levels of one protein and promote another.

<p>False (B)</p> Signup and view all the answers

Riboswitches can respond to uncharged tRNAs through the aminoacyl tRNA synthetase gene.

<p>True (A)</p> Signup and view all the answers

A gene's transcripts in bacteria can directly sense changes in metabolite concentration.

<p>True (A)</p> Signup and view all the answers

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