Normalization in Gene Expression Analysis
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Questions and Answers

What is the standard file format typically used to output gene expression data in RNA sequencing?

  • BAM
  • FASTQ
  • VCF
  • TSV (correct)
  • Which step in RNA sequencing involves organizing and tallying all reads mapped to a specific gene or transcript?

  • Allele fraction calculation
  • Alignment output
  • Gene count (correct)
  • Mutational effects prediction
  • What is the main goal of differential expression analysis in RNA sequencing?

  • Detecting anomalies in the reference genome
  • Predicting allele frequencies
  • Comparing gene expression levels between different conditions (correct)
  • Identifying mutations in the sample
  • Which type of file contains base call alignments to reference sequences in the context of RNA sequencing?

    <p>BAM</p> Signup and view all the answers

    What type of data can impact the calling of mutations in a sample during RNA sequencing?

    <p>Library preparation technique</p> Signup and view all the answers

    Which step is crucial for identifying anomalies that do not match the reference genome in RNA sequencing?

    <p>(Differential) expression data analysis</p> Signup and view all the answers

    What is the primary function of the secondary data analysis step in RNA-Seq?

    <p>To align reads to a reference transcriptome and quantify gene expression</p> Signup and view all the answers

    What is the purpose of the read cleanup step in RNA-Seq data analysis?

    <p>To remove low-quality sequence reads and trim low-quality bases</p> Signup and view all the answers

    Which of the following is an output of the demultiplexing step in RNA-Seq data analysis?

    <p>Multiple FASTQ files, each corresponding to a unique sample</p> Signup and view all the answers

    What is the purpose of sequence alignment in RNA-Seq data analysis?

    <p>To match each sequencing read to its corresponding location in the reference transcriptome</p> Signup and view all the answers

    What is the purpose of the Real-Time Analysis (RTA) software in RNA-Seq data generation?

    <p>To generate base calls and associated quality scores during sequencing</p> Signup and view all the answers

    Which of the following is not a primary processing step in RNA-Seq data analysis?

    <p>Sequence alignment to a reference transcriptome</p> Signup and view all the answers

    What is the purpose of normalization in RNA-Seq data analysis?

    <p>To account for technical biases and make gene expression levels comparable across samples</p> Signup and view all the answers

    Which software is commonly used for differential expression analysis in RNA-Seq?

    <p>Cufflinks</p> Signup and view all the answers

    What is the purpose of using different probability distributions in differential expression analysis?

    <p>To account for different types of biases in the data</p> Signup and view all the answers

    Which of the following is NOT a common challenge in RNA-Seq data analysis?

    <p>Aligning short reads to a reference genome</p> Signup and view all the answers

    What is the purpose of spiking in known quantities of nucleic acid in RNA-Seq experiments?

    <p>To serve as internal controls for normalization</p> Signup and view all the answers

    Which organization proposed a widely accepted nomenclature for reporting mutations or sequence variants?

    <p>Human Genome Variation Society (HGVS)</p> Signup and view all the answers

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