Podcast
Questions and Answers
What principle allows the near-field scanning optical microscope (NSOM) to bypass the diffraction limit?
What principle allows the near-field scanning optical microscope (NSOM) to bypass the diffraction limit?
What determines the resolution of a SNOM?
What determines the resolution of a SNOM?
How does the SNOM ensure that it only probes one pixel at a time?
How does the SNOM ensure that it only probes one pixel at a time?
What is a step involved in preparing the SNOM tip?
What is a step involved in preparing the SNOM tip?
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Why are evanescent waves significant in the context of NSOM?
Why are evanescent waves significant in the context of NSOM?
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In NSOM, multiple exposures to UV light and protein adsorption can be performed. What purpose does this serve?
In NSOM, multiple exposures to UV light and protein adsorption can be performed. What purpose does this serve?
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What role do Maxwell's equations play in the context of evanescent waves in NSOM?
What role do Maxwell's equations play in the context of evanescent waves in NSOM?
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Which of the following statements about SNOM is incorrect?
Which of the following statements about SNOM is incorrect?
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Study Notes
Near-field Scanning Optical Microscope (NSOM/SNOM)
- NSOM/SNOM is a sub-diffraction technique, not limited by the diffraction limit.
- It uses "near-field" evanescent waves to overcome the diffraction limit.
- Evanescent waves arise from the boundary continuity of Maxwell's equations for electromagnetic radiation.
- The technique involves scanning a tip close to the sample surface.
- Light intensity measurements at the end of the tip provide surface data, one pixel at a time.
NSOM/SNOM Tip
- Tip aperture determines the resolution of the SNOM.
- Tips are often created by etching a glass fiber, coating the end with a reflective metal, and then cutting the end.
- The tip is brought close to the sample surface allowing interaction with evanescent waves.
- Multiple UV exposure and protein adsorption cycles are possible with different fluorescent proteins each time.
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Description
This quiz explores the principles and techniques of Near-field Scanning Optical Microscopy (NSOM/SNOM). Learn about how NSOM utilizes evanescent waves to overcome diffraction limits, the importance of the tip aperture, and the processes involved in tip creation and surface data measurement.
