Nature 473, 337 (2011) WS 2023/4 - Lecture 5 Cellular Programs
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Questions and Answers

What is the basis for classifying the cell cycle state in the given experiment?

  • Phosphorylation events
  • Microtubule polymerization
  • DNA content (correct)
  • Protein abundance
  • Why is Nocodazole block considered efficient in synchronizing cells?

  • It alters protein abundance
  • It interferes with microtubule polymerization (correct)
  • It affects phosphorylation events
  • It enhances DNA content
  • What was the threshold used for subsequent analysis in the experiment?

  • 4-fold change in protein abundance (correct)
  • 5-fold change in microtubule polymerization
  • 3-fold change in phosphorylation events
  • 2-fold change in protein abundance
  • How was protein abundance monitored in Experiment 1?

    <p>Using mass spectrometry</p> Signup and view all the answers

    Which technique was used to obtain information from the 6 stages of the cell cycle?

    <p>SILAC peptide triplet</p> Signup and view all the answers

    What was the purpose of quantifying cell cycle markers in different cell populations?

    <p>To understand changes in protein abundance throughout the cell cycle</p> Signup and view all the answers

    What is the purpose of SILAC labeling in the context of the study mentioned?

    <p>To quantify protein expression levels across different stages of the cell cycle.</p> Signup and view all the answers

    Which statement accurately explains the difference between the high-turnover and low-turnover proteins in terms of H/L ratios?

    <p>The H-concentration of high-turnover protein increases over time.</p> Signup and view all the answers

    In the context of the study, what was the role of thymidine in HeLa cell synchronization?

    <p>Blocking cells from entering the S phase of the cell cycle.</p> Signup and view all the answers

    Why were 3 different isotopic forms of arginine and lysine used for SILAC labeling in HeLa cells?

    <p>To allow differentiation between 3 distinct cell populations.</p> Signup and view all the answers

    Which technique was used to quantify protein expression levels in the study discussed?

    <p>Mass Spectrometry</p> Signup and view all the answers

    What was the role of nocodazole in the study involving HeLa cells?

    <p>To block entry into a specific phase of the cell cycle.</p> Signup and view all the answers

    What is the significance of low occupancy phosphorylation of a protein?

    <p>It indicates that only a fraction of the protein copies are phosphorylated at any given time.</p> Signup and view all the answers

    What technique was used to analyze all proteins modified by phosphorylation during different stages of the cell cycle in human HeLa cells?

    <p>Mass spectrometry</p> Signup and view all the answers

    What does SILAC stand for in the context of protein quantification?

    <p>Stable Isotope Labeling by Amino Acids in Cell Culture</p> Signup and view all the answers

    What happens when non-labelled cells are transferred to heavy SILAC growth medium during protein turnover quantification?

    <p>Newly synthesized proteins remain in light form and preexisting proteins incorporate heavy label.</p> Signup and view all the answers

    How many phosphorylated proteins were identified during the analysis of the phospho-proteome in human HeLa cells?

    <p>6695</p> Signup and view all the answers

    What proportion of all proteins was found to be phosphorylated based on the findings presented?

    <p>~70%</p> Signup and view all the answers

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