Podcast
Questions and Answers
What is the difference between hyphae and pseudohyphae?
What is the difference between hyphae and pseudohyphae?
- Hyphae are formed by budding, while pseudohyphae are not.
- Hyphae are found in bacteria, while pseudohyphae are found in fungi.
- Hyphae are tube-like structures, while pseudohyphae are rod-shaped. (correct)
- Hyphae are formed by mitosis, while pseudohyphae are formed by meiosis.
Which phylum produces ascospores in sexual reproduction?
Which phylum produces ascospores in sexual reproduction?
- Mucorales
- Ascomycota (correct)
- Zygomycota
- Basidiomycota
What does the term 'Synanamorphs' refer to?
What does the term 'Synanamorphs' refer to?
- More than one anamorph for the same teleomorph or when a single fungus produces more than one anamorph. (correct)
- Process of sexual reproduction in fungi.
- Asexual reproductive state of a fungus.
- Budding process in fungi.
Which of the following is NOT a mode of transmission for fungal infections?
Which of the following is NOT a mode of transmission for fungal infections?
Which of the following statements accurately describes the term 'colonization' in relation to fungal infections?
Which of the following statements accurately describes the term 'colonization' in relation to fungal infections?
What is the most important factor to consider when collecting a fungal specimen?
What is the most important factor to consider when collecting a fungal specimen?
Which of the following is the best way to collect a blood sample for fungal culture?
Which of the following is the best way to collect a blood sample for fungal culture?
What is the primary purpose of collecting fungal specimens?
What is the primary purpose of collecting fungal specimens?
What is the recommended transport temperature for a corneal scraping specimen?
What is the recommended transport temperature for a corneal scraping specimen?
Which of the following methods are NOT used for temporary mounts?
Which of the following methods are NOT used for temporary mounts?
What is the recommended transport time for a hair specimen?
What is the recommended transport time for a hair specimen?
What is the purpose of using DMSO in the KOH preparation?
What is the purpose of using DMSO in the KOH preparation?
What is the recommended collection method for a prostatic fluid specimen?
What is the recommended collection method for a prostatic fluid specimen?
Why is it important to transport a swab sample for an ear infection immediately?
Why is it important to transport a swab sample for an ear infection immediately?
What is the recommended collection method for a vitreous fluid specimen?
What is the recommended collection method for a vitreous fluid specimen?
Which specimen type can be transported for up to 72 hours at room temperature?
Which specimen type can be transported for up to 72 hours at room temperature?
What are long strands of tube-like structures in fungi called?
What are long strands of tube-like structures in fungi called?
Which characteristic is NOT true regarding the cell structure of fungi?
Which characteristic is NOT true regarding the cell structure of fungi?
In what mode of reproduction do fungi produce spores asexually?
In what mode of reproduction do fungi produce spores asexually?
Which type of mycelia is responsible for nutrient absorption?
Which type of mycelia is responsible for nutrient absorption?
What is a key feature of fungi regarding their oxygen requirement?
What is a key feature of fungi regarding their oxygen requirement?
Which type of hyphae has an antler-shaped appearance?
Which type of hyphae has an antler-shaped appearance?
Which of the following is NOT a portal of entry for fungi?
Which of the following is NOT a portal of entry for fungi?
Which type of hyphae has a club-shaped appearance?
Which type of hyphae has a club-shaped appearance?
What is the primary purpose of using lactophenol blue in specimen preparation?
What is the primary purpose of using lactophenol blue in specimen preparation?
What should be done to the surface before scraping for fungal element collection?
What should be done to the surface before scraping for fungal element collection?
Which of the following statements about urine specimen collection is correct?
Which of the following statements about urine specimen collection is correct?
Which permanent mount stain is useful for differentiating Nocardia from Actinomyces?
Which permanent mount stain is useful for differentiating Nocardia from Actinomyces?
What color does the background appear when using Gomori Methenamine Silver Nitrate?
What color does the background appear when using Gomori Methenamine Silver Nitrate?
Which staining technique is primarily used to detect Histoplasma capsulatum?
Which staining technique is primarily used to detect Histoplasma capsulatum?
What is the fungal element appearance under the India Ink / Nigrosin method?
What is the fungal element appearance under the India Ink / Nigrosin method?
What color do carbohydrates in a fungal wall stain using PAS appear?
What color do carbohydrates in a fungal wall stain using PAS appear?
Which of the following types of hyphae is characterized by frequent cross walls?
Which of the following types of hyphae is characterized by frequent cross walls?
What type of fungi grows both in mold and yeast forms within the same culture?
What type of fungi grows both in mold and yeast forms within the same culture?
What is the primary method of reproduction for yeast organisms?
What is the primary method of reproduction for yeast organisms?
Which type of conidia is formed by the fragmentation of fertile hyphae?
Which type of conidia is formed by the fragmentation of fertile hyphae?
Which structure is a result of asexual reproduction in fungi?
Which structure is a result of asexual reproduction in fungi?
At what temperature do yeast typically grow, especially in increased CO2 concentrations?
At what temperature do yeast typically grow, especially in increased CO2 concentrations?
What type of hyphae is known for possessing few cross walls at irregular intervals?
What type of hyphae is known for possessing few cross walls at irregular intervals?
Which type of spore is formed within a structure called a sporangium?
Which type of spore is formed within a structure called a sporangium?
What is the term for a fungus that reproduces sexually?
What is the term for a fungus that reproduces sexually?
What color of hyphae is indicated by the term 'phaeoid'?
What color of hyphae is indicated by the term 'phaeoid'?
What are the characteristic features of Basidiomycota?
What are the characteristic features of Basidiomycota?
In the collection of sterile fluids, which fluid is not typically included?
In the collection of sterile fluids, which fluid is not typically included?
What is the recommended transport condition for Mucorale specimens?
What is the recommended transport condition for Mucorale specimens?
Which aspect of Fungi Imperfecti is accurate?
Which aspect of Fungi Imperfecti is accurate?
How should bone marrow be collected for testing?
How should bone marrow be collected for testing?
What is the primary mode of asexual reproduction in Mucorales?
What is the primary mode of asexual reproduction in Mucorales?
What is the recommended collection process for catheter samples?
What is the recommended collection process for catheter samples?
What is the collection and transport time guideline for Microsporum spp. and Trichophyton spp.?
What is the collection and transport time guideline for Microsporum spp. and Trichophyton spp.?
Flashcards
Hyphae
Hyphae
Long, thread-like structures that make up the body of a fungus. They can be aerial (above the surface) or vegetative (below the surface).
Mycelia
Mycelia
A dense mass of intertwined hyphae that forms the main body of a fungus.
Aerial Mycelia
Aerial Mycelia
Hyphae that grow above the surface of the culture media, giving a fuzzy or wooly appearance to the fungus.
Vegetative Mycelia
Vegetative Mycelia
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Racquet Hyphae
Racquet Hyphae
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Spiral Hyphae
Spiral Hyphae
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Dimorphism
Dimorphism
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Chitin
Chitin
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Anamorph
Anamorph
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Pseudohyphae
Pseudohyphae
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Synanamorphs
Synanamorphs
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Ascospores
Ascospores
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Basidiospores
Basidiospores
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Zygospores
Zygospores
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Colonization
Colonization
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Infection
Infection
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Rhizoids
Rhizoids
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Yeast
Yeast
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Septate Hyphae
Septate Hyphae
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Sparsely Septate Hyphae
Sparsely Septate Hyphae
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Molds
Molds
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Polymorphic Fungi
Polymorphic Fungi
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Hyaline Hyphae
Hyaline Hyphae
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Phaeoid Hyphae
Phaeoid Hyphae
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Asexual Reproduction
Asexual Reproduction
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Microsporum spp, Trichophyton spp
Microsporum spp, Trichophyton spp
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Scedosporium boydii
Scedosporium boydii
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Basidiomycota
Basidiomycota
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Mucorales
Mucorales
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Fungi Imperfecti
Fungi Imperfecti
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Skin
Skin
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Hair
Hair
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Nails
Nails
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Direct Microscopic Examination
Direct Microscopic Examination
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Permanent Mounts
Permanent Mounts
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Temporary Mounts
Temporary Mounts
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KOH Preparation
KOH Preparation
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KOH with Calcofluor White
KOH with Calcofluor White
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Modified KOH with DMSO
Modified KOH with DMSO
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Ear, External Swab
Ear, External Swab
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Corneal Scraping
Corneal Scraping
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Fungal Stains
Fungal Stains
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Lactophenol Blue
Lactophenol Blue
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PAS (Periodic Acid Schiff)
PAS (Periodic Acid Schiff)
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India Ink / Nigrosin
India Ink / Nigrosin
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Gomori Methenamine Silver Nitrate
Gomori Methenamine Silver Nitrate
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Giemsa
Giemsa
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Gram Stain
Gram Stain
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Acid-Fast Stain
Acid-Fast Stain
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Study Notes
Mycology Part 1 Outline
- General Characteristics:
- Yeasts vs. Molds: Molds have hyphae and mycelia, while yeasts are single-celled.
- Mycelia: A mass of hyphae (long, tubular structures).
- Hyphae: Can be septate (cross-walls) or aseptate (no cross-walls).
- Pigmentation: Hyaline (non-pigmented) or phaeoid (darkly pigmented) hyphae.
- Dimorphism: Some fungi exist as both yeast and mold.
Reproduction
-
Asexual Reproduction:
- Conidia: Asexual reproductive structures formed following mitosis.
- Arthroconidia: Conidia formed by fragmentation of hyphae.
- Phialoconidia: Conidia formed from conidiogenous cells (phialides).
- Blastoconidia: Formed by budding.
- Sporangiospores: Enclosed in a sporangium.
-
Sexual Reproduction (Perfect Fungi):
- Requires two different mating types (plus and minus).
- Involves the fusion of two compatible nuclei leading to a zygospore.
- Teleomorph: Sexual reproductive stage.
- Anamorph: Asexual reproductive stage.
Taxonomy
- Ascomycota: Sexual spores are ascospores.
- Basidiomycota: Sexual spores are basidiospores.
- Zygomycota: Sexual spores are zygospores.
- Fungi Imperfecti (Deuteromycota): No known sexual stage.
Portal of Entry
- Common Entry Points: Skin, hair, nails, respiratory tract, gastrointestinal tract, urinary tract, etc.
Fungal Culture Process
- Specimen Collection and Transport:
- Sterile techniques are important for specimen collection, transportation.
- Various collection methods for different specimens (blood, CSF, fluids, tissue biopsies, etc.).
- Transportation mediums and time limits for maintaining specimen integrity are crucial.
Direct Microscopic Examination
- KOH Preparation: This technique helps to clear away skin cells and tissues, allowing observation of fungal structures.
- Temporary Mounts: Techniques like Lactophenol Cotton Blue help preserve the sample and stain the cell walls for easier visualization.
- Permanent Mounts: Techniques (e.g., Gram, PAS, Giemsa, Silver Nitrate, Acridine Orange) used to prepare stained slides for long-term storage and analysis.
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