Mycology Part 1 Overview

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Questions and Answers

What is the difference between hyphae and pseudohyphae?

  • Hyphae are formed by budding, while pseudohyphae are not.
  • Hyphae are found in bacteria, while pseudohyphae are found in fungi.
  • Hyphae are tube-like structures, while pseudohyphae are rod-shaped. (correct)
  • Hyphae are formed by mitosis, while pseudohyphae are formed by meiosis.

Which phylum produces ascospores in sexual reproduction?

  • Mucorales
  • Ascomycota (correct)
  • Zygomycota
  • Basidiomycota

What does the term 'Synanamorphs' refer to?

  • More than one anamorph for the same teleomorph or when a single fungus produces more than one anamorph. (correct)
  • Process of sexual reproduction in fungi.
  • Asexual reproductive state of a fungus.
  • Budding process in fungi.

Which of the following is NOT a mode of transmission for fungal infections?

<p>Direct contact with infected animals (A)</p> Signup and view all the answers

Which of the following statements accurately describes the term 'colonization' in relation to fungal infections?

<p>Colonization occurs when a fungus multiples within a host, without causing harm. (A)</p> Signup and view all the answers

What is the most important factor to consider when collecting a fungal specimen?

<p>Keeping the specimen moist. (C)</p> Signup and view all the answers

Which of the following is the best way to collect a blood sample for fungal culture?

<p>Collect as bacterial cultures, disinfect with tincture of iodine, and use the maximum amount of blood recommended. (C)</p> Signup and view all the answers

What is the primary purpose of collecting fungal specimens?

<p>To identify the specific fungus causing the infection. (D)</p> Signup and view all the answers

What is the recommended transport temperature for a corneal scraping specimen?

<p>Either Room Temperature or 4°C (C)</p> Signup and view all the answers

Which of the following methods are NOT used for temporary mounts?

<p>Gram Staining (A)</p> Signup and view all the answers

What is the recommended transport time for a hair specimen?

<p>24 hours (A)</p> Signup and view all the answers

What is the purpose of using DMSO in the KOH preparation?

<p>To increase the rate of clearing by breaking down cellular debris (B)</p> Signup and view all the answers

What is the recommended collection method for a prostatic fluid specimen?

<p>Bladder emptying followed by prostatic massage (C)</p> Signup and view all the answers

Why is it important to transport a swab sample for an ear infection immediately?

<p>To prevent the sample from drying out (C)</p> Signup and view all the answers

What is the recommended collection method for a vitreous fluid specimen?

<p>Needle Aspiration (C)</p> Signup and view all the answers

Which specimen type can be transported for up to 72 hours at room temperature?

<p>Nail scraping (B)</p> Signup and view all the answers

What are long strands of tube-like structures in fungi called?

<p>Hyphae (C)</p> Signup and view all the answers

Which characteristic is NOT true regarding the cell structure of fungi?

<p>Chlorophyll present for photosynthesis (A)</p> Signup and view all the answers

In what mode of reproduction do fungi produce spores asexually?

<p>Both B and C (C)</p> Signup and view all the answers

Which type of mycelia is responsible for nutrient absorption?

<p>Vegetative mycelia (C)</p> Signup and view all the answers

What is a key feature of fungi regarding their oxygen requirement?

<p>Obligate aerobes (C)</p> Signup and view all the answers

Which type of hyphae has an antler-shaped appearance?

<p>Antler hyphae (A)</p> Signup and view all the answers

Which of the following is NOT a portal of entry for fungi?

<p>Blood transfusion (C)</p> Signup and view all the answers

Which type of hyphae has a club-shaped appearance?

<p>Racquet hyphae (B)</p> Signup and view all the answers

What is the primary purpose of using lactophenol blue in specimen preparation?

<p>To preserve fungal structures and stain cell walls (B)</p> Signup and view all the answers

What should be done to the surface before scraping for fungal element collection?

<p>Disinfect with 70% alcohol (A)</p> Signup and view all the answers

Which of the following statements about urine specimen collection is correct?

<p>A midstream collection technique should be used (D)</p> Signup and view all the answers

Which permanent mount stain is useful for differentiating Nocardia from Actinomyces?

<p>Acid-Fast Stain (C)</p> Signup and view all the answers

What color does the background appear when using Gomori Methenamine Silver Nitrate?

<p>Green (A)</p> Signup and view all the answers

Which staining technique is primarily used to detect Histoplasma capsulatum?

<p>Giemsa (D)</p> Signup and view all the answers

What is the fungal element appearance under the India Ink / Nigrosin method?

<p>Yeast with a clear halo (C)</p> Signup and view all the answers

What color do carbohydrates in a fungal wall stain using PAS appear?

<p>Magenta (A)</p> Signup and view all the answers

Which of the following types of hyphae is characterized by frequent cross walls?

<p>Septate Hyphae (A)</p> Signup and view all the answers

What type of fungi grows both in mold and yeast forms within the same culture?

<p>Dimorphic Fungi (A)</p> Signup and view all the answers

What is the primary method of reproduction for yeast organisms?

<p>Budding or fission (B)</p> Signup and view all the answers

Which type of conidia is formed by the fragmentation of fertile hyphae?

<p>Arthroconidia (C)</p> Signup and view all the answers

Which structure is a result of asexual reproduction in fungi?

<p>Conidia (D)</p> Signup and view all the answers

At what temperature do yeast typically grow, especially in increased CO2 concentrations?

<p>37°C (A)</p> Signup and view all the answers

What type of hyphae is known for possessing few cross walls at irregular intervals?

<p>Sparsely Septate Hyphae (A)</p> Signup and view all the answers

Which type of spore is formed within a structure called a sporangium?

<p>Sporangiospores (D)</p> Signup and view all the answers

What is the term for a fungus that reproduces sexually?

<p>Teleomorph (B)</p> Signup and view all the answers

What color of hyphae is indicated by the term 'phaeoid'?

<p>Darkly pigmented (D)</p> Signup and view all the answers

What are the characteristic features of Basidiomycota?

<p>Presence of clamp connections (C)</p> Signup and view all the answers

In the collection of sterile fluids, which fluid is not typically included?

<p>Aqueous humor (C)</p> Signup and view all the answers

What is the recommended transport condition for Mucorale specimens?

<p>Never refrigerate (B)</p> Signup and view all the answers

Which aspect of Fungi Imperfecti is accurate?

<p>Includes the largest number of species causing mycoses (A)</p> Signup and view all the answers

How should bone marrow be collected for testing?

<p>Prepared after surgical incision and plated at bedside (B)</p> Signup and view all the answers

What is the primary mode of asexual reproduction in Mucorales?

<p>Sporangiospores (A)</p> Signup and view all the answers

What is the recommended collection process for catheter samples?

<p>Collect 5 cm distal tip of catheter into sterile tubes (A)</p> Signup and view all the answers

What is the collection and transport time guideline for Microsporum spp. and Trichophyton spp.?

<p>≤2 hours at room temperature or ≤24 hours at room temperature (A)</p> Signup and view all the answers

Flashcards

Hyphae

Long, thread-like structures that make up the body of a fungus. They can be aerial (above the surface) or vegetative (below the surface).

Mycelia

A dense mass of intertwined hyphae that forms the main body of a fungus.

Aerial Mycelia

Hyphae that grow above the surface of the culture media, giving a fuzzy or wooly appearance to the fungus.

Vegetative Mycelia

Hyphae that grow below the surface of the culture media, absorbing nutrients for the fungus.

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Racquet Hyphae

Hyphae that have a distinctive swollen or club-shaped appearance.

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Spiral Hyphae

Hyphae that grow in a coiled or spiral shape, giving a distinctive appearance.

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Dimorphism

The ability of some fungi to exist in two different forms: a yeast form (single-celled) and a mold form (multicellular with hyphae).

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Chitin

A substance found in the cell walls of fungi, giving it structural support.

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Anamorph

The asexual reproductive state of a fungus.

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Pseudohyphae

A chain of budding cells that resembles a hypha, but is actually made of individual cells.

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Synanamorphs

When a single fungus produces more than one anamorph or when multiple anamorphs belong to the same teleomorph (sexual stage).

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Ascospores

Sexual spores produced by the Phylum Ascomycota.

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Basidiospores

Sexual spores produced by the Phylum Basidiomycota.

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Zygospores

Sexual spores produced by Zygomycota or Mucorales.

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Colonization

The multiplication of an organism without causing harm to the host.

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Infection

The multiplication of an organism that causes harm and injury to the host.

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Rhizoids

Root-like structures in fungi that lack susceptibility to antibacterial antibiotics.

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Yeast

Single-celled fungi that form smooth, creamy colonies without aerial hyphae. They reproduce through budding or fission.

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Septate Hyphae

Hyphae with frequent cross walls perpendicular to their outer walls.

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Sparsely Septate Hyphae

Hyphae with few cross walls at irregular intervals. Also called coenocytic or aseptate hyphae.

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Molds

Fuzzy or woolly appearance of fungi due to the formation of mycelia. They typically grow at room temperature.

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Polymorphic Fungi

Fungi that can exist in both yeast and mold forms depending on the environment.

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Hyaline Hyphae

Non-pigmented or lightly pigmented hyphae.

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Phaeoid Hyphae

Darkly pigmented hyphae.

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Asexual Reproduction

The process of asexual reproduction in fungi, resulting in the formation of conidia.

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Microsporum spp, Trichophyton spp

A group of fungi that are typically associated with skin, hair, and nail infections. They are commonly known as dermatophytes.

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Scedosporium boydii

A type of fungus that can cause a variety of infections, including skin, lung, and brain infections. Often found in soil and decaying organic matter.

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Basidiomycota

A category of fungi characterized by the presence of clamp connections, which are unique structures found at the septations in the vegetative hyphae.

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Mucorales

A group of fungi that are characterized by sparsely septate hyphae, producing sporangiophores and sporangiospores for asexual reproduction, and zygospores for sexual reproduction.

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Fungi Imperfecti

A category of fungi that lack a known mode of sexual reproduction. They are often referred to as imperfect fungi or Deuteromycota.

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Skin

A common entry point for fungal infections. They are the most common portal of entry for dermatophytes.

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Hair

Fungal infections frequently affect the hair, particularly in cases involving dermatophytes like Microsporum spp. and Trichophyton spp.

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Nails

Fungal infections that affect the nails. They are also a common site for dermatophyte infections.

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Direct Microscopic Examination

A technique used to examine specimens under a microscope by placing a small amount of sample on a glass slide and covering it with a coverslip. This allows for immediate visual inspection of the specimen without culturing.

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Permanent Mounts

This approach involves preserving and mounting a specimen in a medium that allows for long-term observation under a microscope. This is helpful for detailed examination of the specimen's structure and morphology.

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Temporary Mounts

Temporary mounts are useful for the quick examination of specimens to identify features or detect the presence of organisms.

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KOH Preparation

Potassium hydroxide (KOH) is a reagent that helps dissolve keratin and other cell components, making it easier to visualize fungal elements (like hyphae and spores) in specimens.

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KOH with Calcofluor White

This technique involves adding Calcofluor white dye to a KOH preparation, enabling the visualization of fungal elements under a fluorescent microscope. This is a common method for detecting fungal elements with enhanced visibility.

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Modified KOH with DMSO

Dimethyl sulfoxide (DMSO) is a chemical used in a modified KOH procedure. It helps speed up the breakdown of cellular debris, making it easier to see fungi without needing external heat.

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Ear, External Swab

A type of specimen collection that involves swabbing the outer ear canal. The swab is then transported to the lab for further examination and testing.

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Corneal Scraping

A type of specimen collection that involves scraping the cornea of the eye. The scrapings are then inoculated onto growth media and slides prepared for staining.

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Fungal Stains

A technique used to stain fungi in a sample, making them easier to see under a microscope.

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Lactophenol Blue

A type of fungal stain that uses a combination of chemicals to kill fungi, preserve their structure, and stain the cell walls.

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PAS (Periodic Acid Schiff)

A type of fungal stain used to differentiate fungal scrapings from tissue.

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India Ink / Nigrosin

A type of fungal stain used to identify the presence of encapsulated yeast, like Cryptococcus neoformans, in cerebrospinal fluid (CSF).

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Gomori Methenamine Silver Nitrate

A type of fungal stain that causes the fungal element to appear black and the background to appear green.

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Giemsa

A fungal stain used to detect Histoplasma capsulatum, usually in blood or bone marrow.

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Gram Stain

A type of fungal stain that uses crystal violet and ammonium oxalate to stain all fungi.

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Acid-Fast Stain

A fungal stain used to differentiate Nocardia from Actinomyces, showing the hyphae of Nocardia as either partially or fully acid-fast.

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Study Notes

Mycology Part 1 Outline

  • General Characteristics:
    • Yeasts vs. Molds: Molds have hyphae and mycelia, while yeasts are single-celled.
    • Mycelia: A mass of hyphae (long, tubular structures).
    • Hyphae: Can be septate (cross-walls) or aseptate (no cross-walls).
    • Pigmentation: Hyaline (non-pigmented) or phaeoid (darkly pigmented) hyphae.
    • Dimorphism: Some fungi exist as both yeast and mold.

Reproduction

  • Asexual Reproduction:

    • Conidia: Asexual reproductive structures formed following mitosis.
    • Arthroconidia: Conidia formed by fragmentation of hyphae.
    • Phialoconidia: Conidia formed from conidiogenous cells (phialides).
    • Blastoconidia: Formed by budding.
    • Sporangiospores: Enclosed in a sporangium.
  • Sexual Reproduction (Perfect Fungi):

    • Requires two different mating types (plus and minus).
    • Involves the fusion of two compatible nuclei leading to a zygospore.
    • Teleomorph: Sexual reproductive stage.
    • Anamorph: Asexual reproductive stage.

Taxonomy

  • Ascomycota: Sexual spores are ascospores.
  • Basidiomycota: Sexual spores are basidiospores.
  • Zygomycota: Sexual spores are zygospores.
  • Fungi Imperfecti (Deuteromycota): No known sexual stage.

Portal of Entry

  • Common Entry Points: Skin, hair, nails, respiratory tract, gastrointestinal tract, urinary tract, etc.

Fungal Culture Process

  • Specimen Collection and Transport:
    • Sterile techniques are important for specimen collection, transportation.
    • Various collection methods for different specimens (blood, CSF, fluids, tissue biopsies, etc.).
    • Transportation mediums and time limits for maintaining specimen integrity are crucial.

Direct Microscopic Examination

  • KOH Preparation: This technique helps to clear away skin cells and tissues, allowing observation of fungal structures.
  • Temporary Mounts: Techniques like Lactophenol Cotton Blue help preserve the sample and stain the cell walls for easier visualization.
  • Permanent Mounts: Techniques (e.g., Gram, PAS, Giemsa, Silver Nitrate, Acridine Orange) used to prepare stained slides for long-term storage and analysis.

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