Mycobacteriophage D29 and Liposomal Treatment

The time it takes for Mycobacterium tuberculosis (MBT) to double in size.

A type of treatment that uses antibiotics to kill bacteria.

A type of resistance to antibiotics that affects multiple drugs.

A type of virus that specifically infects and kills bacteria.

A type of bacteriophage that destroys bacteria.

A spherical structure that surrounds and protects a substance.

A type of therapy that uses bacteriophages to kill bacteria.

A type of immune response that produces antibodies that neutralize harmful substances.

The ability of a substance, like a bacteriophage, to kill or inhibit the growth of bacteria.

A collection of immune cells, primarily macrophages, that forms around a site of infection to contain the spread of pathogens.

A type of macrophage cell line often used in research to study immune responses. It is useful for assessing the effect of drugs or treatments on these cells.

A method frequently used to separate cells based on their density. Cells are layered over a gradient solution, and different cell types will settle at different levels depending on their weight.

A widely used growth medium suitable for growing mycobacteria, the family of bacteria that includes Mycobacterium tuberculosis. It provides the necessary nutrients for their growth.

Mycobacteriophages are viruses that infect and kill bacteria, specifically Mycobacterium tuberculosis (MTB), the bacterium responsible for tuberculosis.

Mycobacteriophages are being looked at as a promising treatment option for tuberculosis, especially in cases where traditional antibiotics aren't effective.

One challenge in using mycobacteriophages is getting them to the infected areas of the body effectively, where MTB resides.

Liposomes are tiny, spherical sacs that can encapsulate and deliver substances like mycobacteriophages to specific cells or tissues.

The study investigated whether encapsulating mycobacteriophages within liposomes would improve their ability to reach and kill MTB inside macrophages.

Macrophages are white blood cells that engulf and destroy pathogens, including MTB.

The study found that encapsulating mycobacteriophages in liposomes significantly increased their ability to enter macrophages and kill MTB inside them.

Liposomes are a potential way to improve the delivery and effectiveness of mycobacteriophages in treating tuberculosis infections.

A technique used to separate and purify phage particles from a bacterial lysate based on their charge differences.

The process of encapsulating mycobacteriophages within liposomes to enhance their delivery and targeting.

A laboratory model mimicking tuberculosis infection, created by culturing peripheral blood mononuclears from patients with latent or active TB.

A method used to quantify the amount of mycobacteriophages in a sample, employing specific primers and a fluorescent probe.

A ratio used in constructing the tuberculous granuloma model, indicating the number of peripheral blood mononuclears to Mycobacterium tuberculosis bacteria.

The ability of a phage to infect and destroy its host bacteria.

A specific type of phage that targets Mycobacterium smegmatis.