Myco-Composites and Biocomposite Design

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Questions and Answers

What significant characteristic of fungal mycelium contributes to the creation of sustainable composites?

  • High thermal resistance
  • Decomposition of metals
  • Rapid growth (correct)
  • Ability to absorb water

Hybrid-living mycelium composites can be manufactured using conventional mold-based processes.

False (B)

What manufacturing approach is introduced to overcome limitations in the production of myco-composites?

High-resolution biocomposite additive manufacturing

Myco-composites are manufactured utilizing a process that leverages __________ nutritional provision to enhance mycelium growth.

<p>selective</p> Signup and view all the answers

Which of the following factors limit existing hybrid-living mycelium composites?

<p>Conventional mold-based manufacturing processes (B)</p> Signup and view all the answers

Match the myco-composite characteristics with their benefits:

<p>Rapid growth = Faster production time Hydrophobicity = Resistant to water damage Intrinsic regeneration = Sustainability Robust mycelium colonization = Enhanced structure</p> Signup and view all the answers

Myco-composites can only be designed for simple geometries.

<p>False (B)</p> Signup and view all the answers

What is the primary reason cast samples were not tested in compression?

<p>Difficulty in fabricating high-quality compression samples (C)</p> Signup and view all the answers

Anisotropic mechanical properties are influenced by toolpath orientation.

<p>True (A)</p> Signup and view all the answers

What mechanical property was observed to be the highest at a 0º toolpath orientation?

<p>4.88 MPa</p> Signup and view all the answers

Sample geometry and print __________ play a significant role in mechanical performance.

<p>toolpath</p> Signup and view all the answers

Match the following print orientations with their tensile strengths:

<p>0º = 4.88 MPa 90º = 1.45 MPa</p> Signup and view all the answers

What happens to cellulose fibers under shear stress during printing?

<p>They straighten and align (D)</p> Signup and view all the answers

The elastic modulus in tension and compression is different in bulk materials.

<p>False (B)</p> Signup and view all the answers

What does a tangent divergence value of 1 indicate regarding fiber orientation?

<p>Parallel alignment with print direction</p> Signup and view all the answers

Toolpath orientation creates __________ from relatively weak adhesion between parallel paths.

<p>anisotropy</p> Signup and view all the answers

What property of fully colonized composites reduced water absorption?

<p>Hydrophobic mycelial hyphae (A)</p> Signup and view all the answers

Mycelium can bridge gaps up to 10 mm in printed biocomposite panels.

<p>False (B)</p> Signup and view all the answers

What is the main advantage of bio-welded mycelium containers?

<p>They can be opened and resealed as long as the fungi are kept alive.</p> Signup and view all the answers

The addition of mycelium allows for the creation of _____ hinges in biocomposite panels.

<p>flexible</p> Signup and view all the answers

Match the properties of mycelium to their potential benefits:

<p>Insulative = Maintains temperature Flame-resistant = Increases safety Hydrophobic = Reduces water absorption Flexible = Enhances structural performance</p> Signup and view all the answers

What additional feature could a fully coated mycelium skin provide?

<p>Enhanced water resistance (C)</p> Signup and view all the answers

The rigid biocomposite regions in a printed textile-like material bend and stretch more than the mycelium.

<p>False (B)</p> Signup and view all the answers

What nutritional requirement is necessary for the continued growth of mycelium in containers?

<p>Sufficient nutritional media.</p> Signup and view all the answers

The initial water absorption of colonized samples is due to higher _____ levels.

<p>porosity</p> Signup and view all the answers

What unique capability allows mycelium to create waterproof containers?

<p>Bio-welding (D)</p> Signup and view all the answers

Which of the following references discusses advancements in materials science related to myco-composites?

<p>A.Rodrigo-Navarro et al., Nature Reviews Materials, 2021 (C)</p> Signup and view all the answers

The Dunkin’ employees at the Stratton Student Center collected and donated spent coffee grounds for research purposes.

<p>True (A)</p> Signup and view all the answers

Who specifically extended appreciation for assistance with SEM imaging?

<p>Weaver</p> Signup and view all the answers

The reference by M.Jones et al. was published in the journal __________ in 2020.

<p>Mater Des</p> Signup and view all the answers

Match the following publications with their corresponding years:

<p>Prog Mater Sci = 2007 Nature = 2019 Biomaterials Advances = 2022 Sci Total Environ = 2020</p> Signup and view all the answers

Which of the following fungi species was NOT mentioned for mycelium cultivation?

<p>Ganoderma lucidum (C)</p> Signup and view all the answers

The mycelium is cultivated using a 6-quart pressure cooker at 15 psi for 30 minutes.

<p>False (B)</p> Signup and view all the answers

What is one application of myco-composites mentioned in the content?

<p>Novel hybrid-living materials.</p> Signup and view all the answers

The agar solution was prepared using __________ malt extract.

<p>2 wt%</p> Signup and view all the answers

Match the following terms with their descriptions:

<p>P. ostreatus = Oyster mushroom T. versicolor = Turkey tail mushroom Biocompatibility = Compatibility with living tissue Printability = Ability to be fabricated into a structure</p> Signup and view all the answers

What parameter was NOT varied during the composite design?

<p>Temperature (A)</p> Signup and view all the answers

Ecovative culture was purchased as liquid mycelium cultures.

<p>False (B)</p> Signup and view all the answers

How long did it take for the petri dishes to become fully colonized?

<p>Approximately 1 week.</p> Signup and view all the answers

Malt extract agar plates should be __________ before use.

<p>cooled completely</p> Signup and view all the answers

What is the significance of computation-assisted approaches in biomateriomics?

<p>They speed up material discovery. (A)</p> Signup and view all the answers

Flashcards

Myco-composites

Hybrid-living materials using fungal mycelium and other components.

Biocomposite additive manufacturing

A method to create myco-composites with high precision.

Indirect inoculation

Introducing mycelium to a material without direct contact.

Hierarchical composite design

Designing composites with different layers for strength and function.

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Selective nutritional provision

Providing specific nutrients to control mycelium growth.

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Scalable myco-composite

A myco-composite that can be produced in large quantities.

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Tunable myco-composite

A myco-composite with properties that can be adjusted.

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Biocomposite Mechanical Properties

Mechanical properties (like strength) of biocomposites are influenced by factors like the cellulose proportion and print method.

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Anisotropic Mechanical Properties

Material properties that vary depending on the direction of force application.

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Toolpath Orientation

Direction of printing nozzle's movement relative to testing direction.

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Tensile Strength (Toolpath 0º)

Maximum strength of the material when pulled in the direction parallel to the print path.

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Tensile Strength (Toolpath 90º)

Lowest strength of the material when pulled in the direction perpendicular to the print path.

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Cellulose Fiber Alignment

How cellulose fibers are arranged within the printed material.

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Anisotropy (Microscale)

Variations in material properties within a single filament due to fiber alignment caused by shear stress during extrusion.

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Fiber Alignment Quantification

Measuring fiber alignment using tangent divergence to assess how closely fibers align with the print direction.

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Hierarchical Materials

Materials with multiple levels of organization creating superior properties.

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Mycelium

The network of thread-like structures (hyphae) that make up a fungus.

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Biocomposite

A material made from a combination of a biological material (like mycelium) and a synthetic or natural material.

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Mycelium water absorption

Fully colonized biocomposites absorb less water and swell less than non-colonized ones after submersion.

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Initial swelling cause

Initially high porosity of the material leads to faster water absorption and swelling

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Bio-welding

Mycelium's ability to bridge gaps between materials.

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Bio-welded container

A container made by printing biocomposite panels separated by gaps, then letting the mycelium grow to bridge the gaps, forming flexible hinges.

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Self-sealing container

The bio-welded container seals itself completely after colonization, leveraging mycelium's ability to bridge gaps and grow.

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Waterproof container

Mycelium-sealed containers are waterproof, provided with constant nutrition.

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Sustainable packaging

Mycelium-based packaging is a potentially sustainable alternative to traditional methods, due to its insulative and flame-resistant properties.

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Biocomposite textile

Small islands of biocomposite printed with specific spacing, and then colonized, creating a flexible, textile-like material

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Gap bridging (mycelium)

Mycelium can bridge gaps of up to 8 mm.

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Mycelium properties

Mycelium demonstrates insulative and flame-resistant properties.

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What are myco-composites?

Myco-composites are hybrid-living materials that combine fungal mycelium with other components.

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What makes myco-composites unique?

Myco-composites offer a unique combination of biodegradability, sustainability, and potential for custom design.

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What is the design space for myco-composites?

The design space for myco-composites includes factors like material formulation, fungal species, and processing parameters.

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What is biomateriomics?

Biomateriomics is the study of using computational approaches to accelerate the discovery and optimization of biological materials.

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Why is biomateriomics important for myco-composites?

Biomateriomics can help speed up the development of myco-composites with specific properties.

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Malt extract agar

A nutrient-rich medium used to grow mycelium in petri dishes.

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How are mycelium cultures prepared?

Mycelium cultures are prepared by inoculating agar plates with liquid or solid mycelium.

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How can composite design be explored for myco-composites?

Parameters like coffee content, hydration level, and acidity can be varied to investigate myco-composite design.

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What are the advantages of using biocomposites?

Biocomposites offer advantages like biodegradability, renewable resources, and potential for sustainable manufacturing.

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What is the future of myco-composites?

The future of myco-composites holds potential for scalable manufacturing, new applications, and further advancements.

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Study Notes

Robust Myco-Composites: A Biocomposite Platform for Versatile Hybrid-Living Materials

  • Abstract: Fungal mycelium, a network of filamentous threads, thrives on lignocellulosic waste. It exhibits rapid growth, hydrophobicity, and intrinsic regeneration, effectively creating next-generation sustainable and functional composites (myco-composites). Existing myco-composites are limited by conventional mold-based manufacturing processes. A novel platform is introduced that uses high-resolution biocomposite additive manufacturing and indirect inoculation for robust and scalable mycelium colonization. These myco-composites show improved properties, such as tensile strength (up to 160 MPa), compared to existing materials.

Biocomposite Design

  • Composition: The composite is designed using minimally processed, non-toxic, and biodegradable materials from waste streams. Chitosan and cellulose are used as the basic materials, offering mechanical properties and abundance in natural structures. Spent coffee grounds are incorporated for sustainable nutritional support of fungal growth.

  • Mechanical properties: The fully infilled 3D-printed composite demonstrates increased Young's modulus compared to cast samples in tension (up to 340 MPa). However, compressive modulus for printed material was lower than cast material due to mechanical defects in the printing.

  • Indirect inoculation: This method of fungal growth was found more effective and robust on 3D-printed structures, filling voids efficiently and displaying high biocompatibility with multiple fungi.

Impacts of Mycelium Colonization

  • Mechanical Properties: Colonization significantly enhances mechanical properties compared to uncolonized composites, including over a fifteen-fold increase in modulus.

  • Wettability and Absorption: Full colonization improved hydrophobicity (increased contact angle). Water absorption, measured after 24 hours, was substantially lower in colonized composites.

Bio-welded Containers and Textiles

  • Versatile applications: The platform enables the creation of foldable bio-welded containers and flexible textile-like structures. This showcases versatility in structural and textile applications. Mycelium's ability to bridge gaps offers potential for sustainable packaging.

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