Muscle Cell Differentiation Genes Quiz

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Questions and Answers

What is the primary purpose of identifying genes expressed in differentiating muscle cells?

  • To determine the genetic causes of muscle atrophy
  • To understand the mechanisms of muscle formation (correct)
  • To find new therapeutic targets for muscle diseases
  • To explain the function of non-muscle cell types

Which of the following genes is NOT mentioned as being involved in muscle differentiation?

  • MyoB (correct)
  • MyoD
  • MyoA
  • MyoH

What role does calcium phosphate play in the experimental procedure described?

  • It aids in the uptake of DNA by cells (correct)
  • It is required for muscle cell differentiation
  • It enhances muscle cell growth
  • It is a nutrient for fibroblast cells

After incubating fibroblast cells with cloned genes, what is the next step in the procedure?

<p>Allow the cells to grow for 3 to 5 days (D)</p> Signup and view all the answers

What is the aim of examining the cells under a microscope in this experiment?

<p>To observe the cellular morphology for differentiation (B)</p> Signup and view all the answers

Which of the following statements is true regarding the hypothesized gene function?

<p>Particular genes are responsible for inducing muscle differentiation. (A)</p> Signup and view all the answers

What type of cells were used as a model for studying gene-induced muscle differentiation?

<p>Fibroblast cells (C)</p> Signup and view all the answers

Which of the following is a potential outcome of the experimental procedure described?

<p>Non-muscle cells acquire muscle cell morphology (A)</p> Signup and view all the answers

What role do transcription factors play in the development of segments during phase 3?

<p>They establish patterns for each segment and control characteristics. (A)</p> Signup and view all the answers

Which characteristic is NOT associated with stem cells?

<p>Permanent functioning as a specific cell type (D)</p> Signup and view all the answers

What is the primary basis for cell differentiation?

<p>Differential gene expression (B)</p> Signup and view all the answers

During phase 4 of development, cells gain specific functions. What is this process primarily known as?

<p>Cell differentiation (A)</p> Signup and view all the answers

What is the significance of studying cell differentiation in mammalian cell culture lines?

<p>It helps to analyze and manipulate gene expression for therapeutic uses. (A)</p> Signup and view all the answers

What is primarily responsible for the specific characteristics of a differentiated cell?

<p>Differential gene expression (C)</p> Signup and view all the answers

Which statement best describes the genome of differentiated cells?

<p>They contain a complete functional set of genes. (D)</p> Signup and view all the answers

What occurs long before a cell actually differentiates?

<p>Commitment to a specific cell type (B)</p> Signup and view all the answers

How does somatic cell nuclear transfer affect the genome?

<p>It reprograms the genome. (D)</p> Signup and view all the answers

What is the role of cell adhesion molecules (CAMs) in a multicellular organism?

<p>They influence cell positioning within the organism. (B)</p> Signup and view all the answers

In the context of development, what is a zygote?

<p>A single diploid cell formed by the union of sperm and egg. (A)</p> Signup and view all the answers

What central question does developmental genetics seek to answer?

<p>How does gene expression control development? (B)</p> Signup and view all the answers

What defines the function of a specialized cell in a multicellular organism?

<p>Specific combinations of proteins (D)</p> Signup and view all the answers

What type of stem cell can produce all adult cell types?

<p>Totipotent (C)</p> Signup and view all the answers

Which type of stem cell has lost the ability to produce an entire individual?

<p>Pluripotent (C)</p> Signup and view all the answers

Which cell type is considered a totipotent stem cell?

<p>Fertilized egg (B)</p> Signup and view all the answers

Embryonic germ cells are classified as which type of stem cell?

<p>Pluripotent (C)</p> Signup and view all the answers

What type of stem cells come from the inner cell mass of a blastocyst?

<p>Embryonic stem cells (D)</p> Signup and view all the answers

Which category of stem cells can differentiate into multiple cell types but not all?

<p>Multipotent (B)</p> Signup and view all the answers

Which of the following does NOT correctly describe pluripotent stem cells?

<p>Are derived from the fertilized egg (B)</p> Signup and view all the answers

How are totipotent stem cells characterized?

<p>Can develop into an entire organism (A)</p> Signup and view all the answers

What type of stem cells can differentiate into any cell type in the body?

<p>Totipotent (C)</p> Signup and view all the answers

Which type of stem cells are found in adult bone marrow?

<p>Multipotent (A)</p> Signup and view all the answers

What characterizes unipotent stem cells?

<p>Can only produce one type of cell (C)</p> Signup and view all the answers

What type of cells are embryonic germ (EG) cells?

<p>Pluripotent (C)</p> Signup and view all the answers

Which of the following best describes hematopoietic stem cells (HSCs)?

<p>They are multipotent and can differentiate into several blood cell types. (D)</p> Signup and view all the answers

Which of the following statements about fetal cells is accurate?

<p>Fetal cells are either multipotent or unipotent. (C)</p> Signup and view all the answers

What defines the inner cell mass of a blastocyst?

<p>It contains pluripotent embryonic stem cells. (C)</p> Signup and view all the answers

Which cell type produces skin cells exclusively?

<p>Unipotent stem cells (C)</p> Signup and view all the answers

What is the primary function of hematopoietic stem cells?

<p>To differentiate into various blood cell types. (D)</p> Signup and view all the answers

Which cells are derived from myeloid cells?

<p>Osteoclasts and macrophages. (A), Platelets and neutrophils. (D)</p> Signup and view all the answers

Which of the following is NOT a type of cell that a hematopoietic stem cell can differentiate into?

<p>Cardiomyocyte (D)</p> Signup and view all the answers

Hematopoietic stem cells are located primarily in which part of the body?

<p>Bone marrow (A)</p> Signup and view all the answers

What are the two main pathways that hematopoietic stem cells can take during differentiation?

<p>Myeloid and lymphoid. (C)</p> Signup and view all the answers

Which type of lymphoid cell is responsible for adaptive immunity?

<p>T cell (C)</p> Signup and view all the answers

What role do dendritic cells play in the immune system?

<p>They present antigens to T cells. (A)</p> Signup and view all the answers

Which of the following cells is generated from the differentiation of hematopoietic stem cells into the myeloid lineage?

<p>Basophil (D)</p> Signup and view all the answers

Flashcards

Cell Differentiation

The process by which a cell acquires specialized characteristics, becoming a specific cell type.

Developmental Genetics

The study of how genes control the process of development, including cell differentiation and pattern formation.

Differential Gene Expression

Specialized cells contain specific proteins that allow them to perform their unique functions. These proteins are produced by the differential expression of genes.

Complete Functional Set of Genes

All cells in a multicellular organism have the same set of genes, but they express different genes depending on their function, leading to specialization.

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Somatic Cell Nuclear Transfer

A process where the nucleus of a differentiated cell is transferred into an enucleated egg cell, potentially reprogramming the genome to become pluripotent.

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Pluripotent Stem Cells

Cells that are capable of developing into any cell type in the body, like embryonic stem cells.

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Reprogramming

The process of re-programming a differentiated cell back into a pluripotent state, allowing it to develop into different cell types.

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Reprogramming for Transplantation

The use of reprogramming techniques to create tissues for transplantation, potentially offering solutions for treating diseases and injuries.

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Stem cell characteristics

The ability of a stem cell to divide and produce daughter cells that can become various cell types, leading to diverse tissues and organs.

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Pattern formation

Stage in development where cells are organized into patterns and structures based on their position within the embryo.

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Transcription factors

Proteins that bind to DNA and control gene expression, regulating the development and differentiation of cells.

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Totipotent

The initial cell formed by the fusion of a sperm and egg, capable of developing into any cell type in the body.

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Pluripotent

A type of stem cell found in the inner cell mass of a blastocyst (early embryo), capable of developing into almost any cell type in the body.

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Multipotent

Stem cells that can differentiate into multiple cell types, but are limited in their potential compared to totipotent or pluripotent cells.

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Unipotent

Stem cells that can only differentiate into one type of cell, like a blood stem cell that only forms red blood cells.

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Embryonic Stem Cells

Stem cells found in the early embryo, capable of developing into almost any cell type.

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Adult Stem Cells

Stem cells found in adult tissues, capable of differentiating into a limited number of cell types.

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Stem Cell

A cell that can renew itself and produce daughter cells that can specialize into different cell types.

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Hematopoietic Stem Cells

A specific type of adult stem cell in bone marrow that produces various blood cells.

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Cell division

The process by which a stem cell divides and differentiates, becoming a specific cell type.

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Myeloid cell

Stem cells that can differentiate into various blood cells, including red blood cells, white blood cells, and platelets.

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Lymphoid cell

Stem cells that differentiate into lymphocytes, responsible for immune responses.

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White blood cells

Cells that develop from myeloid stem cells and are crucial for fighting infections and inflammation.

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Platelets

Fragments of large cells that help blood clotting and wound healing.

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Master Transcription Factors

Proteins that control the activation of other genes, which in turn control the development of specific cell types. They are essential for guiding cells down a specific developmental pathway.

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Identifying Muscle Differentiation Genes

An experiment designed to identify specific genes responsible for the differentiation of muscle cells. The experiment involved isolating and introducing genes expressed only in differentiating muscle cells into non-muscle cells (fibroblasts) and observing whether they induced muscle cell development.

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Three Candidate Genes

The experiment used three cloned genes, named MyoA, MyoD, and MyoH, which were expressed only in differentiating muscle cell lines. These genes were introduced into fibroblasts (non-muscle cells) to assess their potential to induce muscle differentiation.

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Can Genes Induce Muscle Differentiation?

The central question of this experiment was to determine if any of the three candidate genes (MyoA, MyoD, and MyoH) could trigger the differentiation of non-muscle cells (fibroblasts) into muscle cells.

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Introducing Genes to Fibroblasts

The experiment involved introducing each candidate gene (MyoA, MyoD, or MyoH) into fibroblasts (non-muscle cells) using a calcium phosphate method. This promotes the uptake of DNA into the cells, allowing the cells to express the introduced gene.

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Growing Cells with Candidate Genes

The fibroblasts were then grown in a culture medium for several days. This allowed the introduced genes to express and potentially induce changes in the cells' morphology and function.

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Microscopic Examination for Muscle Cell Morphology

The experiment involved examining the fibroblasts under a microscope to determine if they had developed the characteristic morphology of differentiating muscle cells. Any significant changes in the cells' appearance would suggest successful muscle differentiation.

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The Significance of the Experiment

This experiment aimed to prove or disprove the hypothesis that particular genes are responsible for inducing muscle differentiation. The results of this experiment could provide significant insights into the genetic mechanisms underlying muscle development and could lead to new approaches for treating muscle-related diseases.

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Study Notes

Cell Differentiation

  • Cell differentiation is a series of changes in the state of a cell, tissue, organ, or organism.
  • The process gives rise to the structure and function of living organisms.
  • Developmental genetics studies how gene expression controls this process.

General Themes in Development

  • Sperm and egg unite to create a zygote.
  • The diploid zygote develops into an embryo.
  • Cells divide and arrange themselves.
  • Cells become determined, meaning they are destined to become a particular cell type.
  • Commitment to becoming a specific cell type occurs long before the cell differentiates.

Genome and Development

  • The genome is a set of genes that constitute the program of development..
  • In unicellular organisms, the genome controls the structure and function of the single cell.
  • In multicellular organisms, the genome controls cellular features and the arrangement of cells.

Cell Adhesion

  • Animal cells create their own cell adhesion molecules (CAMs).
  • Cell positioning within a multicellular organism depends on interactions with other cells and the extracellular matrix.
  • Cell-to-cell contact provides positional information.
  • Transcription factors, made of proteins, are a key driving force for cell differentiation.

Hierarchy of Transcription Factors

  • Body formation occurs in four phases:
    • Organising body along major axes.
    • Organising into smaller regions (organs, legs).
    • Cells changing morphology to become differentiated.
  • Differential gene regulation means certain genes are expressed at specific phases of development for a particular cell type.
  • Specific transcription factors are expressed during each phase of body formation.
  • Transcription factors in Phase 1 determine the formation of body axes.
  • Transcription factors in Phase 2 subdivide the embryo into regions with specific properties.
  • Transcription factors in Phase 3 dictate segment and group development.
  • Transcription factors in Phase 4 cause cells differentiate into specific types (skin, nerve, muscle).

Phase 4 - Cell Differentiation

  • Once patterns are established, cells differentiate to carry out their roles.
  • Cell differentiation is studied using mammalian cell culture lines.
  • Differential gene expression is the basis of cell differentiation.
  • Stem cells have the capacity to divide.
  • Daughter cells can differentiate into various cell types.

Stem Cell Categories

  • Totipotent: The ultimate stem cell—the fertilized egg—can produce all adult cell types.
  • Pluripotent: Embryonic stem cells (ES cells) and embryonic germ cells (EG cells) can differentiate into almost any cell type but cannot create a complete individual.
  • Multipotent: Hematopoietic stem cells (HSCs) can differentiate into several blood cell types.
  • Unipotent: Stem cells in skin can only produce skin cells.

Some Potential Uses of Stem Cells in Disease Treatment

  • Nerve: Implanted cells can treat Parkinson's disease and spinal cord injuries.
  • Skin: Used in burn treatments and skin disorders.
  • Cardiac: Used to repair heart damage after heart attacks.
  • Cartilage: Used for repair of damaged joints from injury or arthritis.
  • Bone: Used to repair damaged bone.
  • Liver: Used to repair or replace damaged liver tissue.
  • Skeletal muscle: Used to repair or replace damaged muscle tissue.

Genes That Promote Muscle Cell Differentiation

  • Certain proteins act as master transcription factors to differentiate stem cells into specific cell types.
  • Initial experiments focused on muscle differentiation.
  • Three specific genes (MyoA, MyoD, MyoH) are key.
  • These identified genes were responsible for causing muscle cells to become differentiated.
  • MyoD is the only gene that can cause fibroblasts to differentiate into muscle cells. MyoD belongs to the myogenic bHLH gene family that is found in all vertebrates and activated during skeletal muscle development. MyoD plays a key role in skeletal muscle differentiation because its basic domain binds to an enhancer DNA sequence that is adjacent to genes expressed only in muscle cells.

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