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Questions and Answers

What is the region of the cell that contacts the extracellular matrix (ECM) called?

  • Apical pole
  • Lateral pole
  • Basal pole (correct)
  • Reticular pole

Which part of the basement membrane is closest to the epithelial cells?

  • Basal lamina (correct)
  • Cytoplasmic layer
  • Reticular lamina
  • Interstitial layer

What type of collagen is primarily found in the reticular lamina?

  • Type II collagen
  • Type I collagen
  • Type IV collagen
  • Type III collagen (correct)

What component is predominantly found in the basal lamina and helps in the attachment to transmembrane integrin proteins?

<p>Laminin (A)</p> Signup and view all the answers

Which type of collagen is necessary for anchoring fibrils in the reticular lamina?

<p>Type VII collagen (A)</p> Signup and view all the answers

The function of the basement membrane includes which of the following?

<p>Filtering molecules (D)</p> Signup and view all the answers

What distinguishes the apical pole from the basal pole in epithelial cells?

<p>Their contact with the ECM (B)</p> Signup and view all the answers

Which of the following is NOT a component of the basal lamina?

<p>Elastin (A)</p> Signup and view all the answers

What are the four basic tissue types in the human body?

<p>Epithelial, connective, muscular, and nervous tissues (D)</p> Signup and view all the answers

Which function is NOT associated with epithelial tissues?

<p>Contraction (C)</p> Signup and view all the answers

What characterizes muscle tissue as opposed to epithelial tissue?

<p>It has elongated cells specialized for contraction. (D)</p> Signup and view all the answers

What is the main role of the stroma in organs?

<p>To provide structural support to the organ. (C)</p> Signup and view all the answers

Which type of epithelial cells can be contractile?

<p>Myoepithelial cells (B)</p> Signup and view all the answers

Why do epithelial cells typically not contain blood vessels?

<p>They receive nutrients and oxygen from adjacent connective tissue. (C)</p> Signup and view all the answers

What describes the extracellular matrix (ECM) in epithelial tissues?

<p>Thin and supportive. (A)</p> Signup and view all the answers

Which statement about epithelial cell polarity is true?

<p>Epithelial cells show polarity with uneven distribution of organelles and proteins. (D)</p> Signup and view all the answers

What is the primary function of junctional adhesive molecules (JAMs)?

<p>Support the structure of tight junctions. (A)</p> Signup and view all the answers

Which bacterium is known to disrupt tight junctions by targeting claudin molecules?

<p>Clostridium perfringens (D)</p> Signup and view all the answers

What role do E-cadherins play in adherens junctions?

<p>They mediate cell adhesion. (C)</p> Signup and view all the answers

How do desmosomes differ from zonula adherens?

<p>Desmosomes are disc-shaped and form spot-welds. (B)</p> Signup and view all the answers

What distinguishes fascia adherens from zonula adherens?

<p>Fascia adherens does not encircle the entire circumference of the cell. (C)</p> Signup and view all the answers

In the context of gastric ulcers, which protein does Helicobacter pylori target?

<p>ZO-1 (D)</p> Signup and view all the answers

Which junction forms a structural connection that resembles a belt around epithelial cells?

<p>Zonula adherens (B)</p> Signup and view all the answers

What is the primary role of catenins in adherens junctions?

<p>Bind cadherins to actin filaments. (D)</p> Signup and view all the answers

What is one of the primary functions of basement membranes in relation to epithelial cells?

<p>Provide structural support (A)</p> Signup and view all the answers

How do basal lamina components influence epithelial cell functions?

<p>They help organize proteins in the plasma membrane (D)</p> Signup and view all the answers

Which junction type forms a seal between adjacent epithelial cells?

<p>Tight junctions (D)</p> Signup and view all the answers

What role do basement membrane proteins play during cell migration?

<p>They serve as markers for certain cell migrations (B)</p> Signup and view all the answers

What is a characteristic of tight junctions among epithelial cells?

<p>They form a band completely encircling each cell (B)</p> Signup and view all the answers

Which protein family is primarily involved in forming linear fibrils in tight junctions?

<p>Claudins (D)</p> Signup and view all the answers

What is a primary interaction facilitated by occludin in tight junctions?

<p>Interaction with actin filaments (D)</p> Signup and view all the answers

What is a critical role of the basement membrane in epithelial repair?

<p>It serves as a scaffold for rapid repair and regeneration. (C)</p> Signup and view all the answers

Flashcards

Epithelial Tissue

A tissue type composed of closely aggregated polyhedral cells that cover and line body surfaces and cavities, performing functions like absorption, secretion, and protection.

Epithelial Cell Shape

Epithelial cells are typically polyhedral (many sides), arranged tightly together.

Epithelial Function

Epithelial tissues are responsible for covering, lining, protection, absorption, and secretion in the body.

Epithelial Cell Polarity

Proteins and organelles in epithelial cells are distributed unevenly, reflecting different functions at different cell surfaces.

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Basement Membrane

A thin layer of extracellular matrix that holds epithelial cells together and provides structural support.

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Parenchyma

The cells in an organ that perform its specialized function.

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Stroma

The supportive connective tissue in an organ, holding the parenchyma together.

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Basic Tissue Types

The four fundamental types of tissue: epithelial, connective, muscle, and nervous.

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Extracellular Matrix (ECM)

The non-cellular component of tissues, providing structural support and influencing cell function.

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Basal pole of a cell

The region of a cell contacting the extracellular matrix (ECM) and connective tissue.

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Apical pole of a cell

The end of a cell that usually faces a space, often different in structure and function to the basal pole.

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Lateral surface of a cell

The regions of cuboidal or columnar cells that touch neighboring cells.

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Basement Membrane

A thin extracellular structure beneath the epithelium that acts as a semi-permeable filter.

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Basal lamina

The thin, electron-dense sheet-like layer of fine fibrils in the basement membrane, nearest the epithelial cells.

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Reticular lamina

The more diffuse and fibrous layer beneath the basal lamina in the basement membrane.

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Type IV collagen

A component of the basal lamina that forms a mesh-like network.

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Laminin

A glycoprotein in the basal lamina that attaches to integrin proteins and interacts with type IV collagen.

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Nidogen and perlecan

Components in the basal lamina that cross-link laminin to type IV collagen and influence structure and filtering properties.

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Type III collagen

A component of the reticular lamina, a part of the basement membrane.

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Type VII collagen

A component of the reticular lamina that anchors it to the basal lamina via anchoring fibrils.

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Basement Membrane Function

Basement membrane acts as a structural support for epithelial cells, attaching them to connective tissues.

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Basement Membrane Organization

Basement membrane proteins organize cell proteins in the cell membrane and help cells maintain their structure, direction, and pathways.

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Cell-to-Cell Interactions

Basement membrane proteins guide and support cell-to-cell interactions within and around epithelia.

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Epithelial Repair and Regeneration

Basement membrane proteins provide a scaffold for tissue repair and growth.

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Tight Junctions

Tight junctions, also known as zonula occludens, seal epithelial cells together, preventing the passage of substances between cells.

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Adherent Junctions

Adherent junctions are areas where cells are firmly attached to each other and/or basal lamina.

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Gap Junctions

Gap junctions act as channels connecting cells, allowing communication.

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Zonula Occludens

Another name for tight junctions in epithelia; belt-like structures around cells.

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Claudin

Proteins that form tight junctions and enable paracellular movement of water.

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Occludin

Transmembrane protein involved in the tight junction formation and function.

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ZO proteins

Proteins that link tight junction proteins and actin filaments to maintain spatial organization and function.

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Nectins

Proteins that contribute to tight junction formation and cell adhesion.

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Junctional Adhesive Molecules (JAMs)

Proteins that help form tight junctions and anchor cells to the cytoskeleton.

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Zonula Occludens (Tight Junction)

A belt-like structure that seals the space between cells, preventing leakage.

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Food Poisoning & Tight Junctions

Bacteria like Clostridium perfringes can disrupt tight junctions causing fluid loss and cell death.

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Adherens Junction (Zonula Adherens)

A band of proteins that firmly anchors cells together.

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Cell Adhesion via Cadherins

Cell adhesion depends on proteins called cadherins, which link neighboring cells together.

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Desmosome

A spot-like junction that provides strong mechanical strength between cells.

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Fascia Adherens

Similar to adherens junctions but does not encircle the entire cell.

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Study Notes

Epithelial Tissue Overview

  • Epithelial tissue is one of the four basic tissue types in the human body
  • It comprises specialized cells grouped together to perform specific functions
  • The tissues are epithelial, connective, muscular, and nervous

Basic Tissue Types

  • Epithelial tissue: Composed of closely packed cells that form sheets or layers. Specialized for secretion, absorption, protection, etc.
  • Connective tissue: Supports and connects other tissues; contains a vast matrix of extracellular material. Highly varied cells for various functions.
  • Muscle tissue: Composed of elongated cells specialized for contraction; involved in movement
  • Nervous tissue: Consists of cells with long processes for communication; Involved in signaling, transmission of nerve impulses

Epithelial Tissue Functions

  • Covering, lining, and protection (epidermis)
  • Absorption (intestinal cells)
  • Secretion (salivary glands)
  • Excretion (kidney)

Specialized Epithelial Cell Types

  • Myoepithelial cells: Can contract
  • Cells from taste buds, olfactory epithelium: Sensory cells

Epithelial Cell Polarity

  • Polarity: Organelles and membrane proteins unevenly distributed in the cells
  • Basal pole: Region adjacent to ECM and connective tissue
  • Apical pole: Usually facing a space; Function and structure differ significantly from basal pole

Epithelial Cell Junctions

  • Tight junctions: Form a water-tight seal between adjacent cells
  • Adherens junctions: Strong cell adhesion sites
  • Desmosomes: Anchoring junctions; resist mechanical stress
  • Gap junctions: Channels for communication between adjacent cells

Basement Membrane

  • A thin extracellular layer between epithelium and connective tissue
  • A semi-permeable filter for reaching epithelial cells from below

Basement Membrane Components

  • Type IV collagen: A two-dimensional network, like a window screen, that attaches to integrin proteins in basal cell membranes
  • Laminin: Large glycoprotein that attaches to transmembrane integrin proteins
  • Nidogen and Perlecan: Cross-link laminins to type IV collagen, provide a 3D structure, and determine porosity/size of filtering molecules

Other specialized features

  • Microvilli: Increase surface area for absorption; abundant in the small intestine
  • Stereocilia: Long, immobile structures that increase surface area for absorption, found in the male reproductive system
  • Cilia: Move substances along the epithelial surface, often found in the respiratory system

Epithelial Tissue Classification

  • Based on the number of cell layers and shapes of cells
  • Simple epithelium: Single layer
  • Stratified epithelium: More than one layer
  • Shapes: Squamous (flattened), Cuboidal (cube-like), Columnar (tall and rectangular)
    • Additional shapes: Pseudostratified (appears stratified but is single), Transitional (varies in shapes depending on stretch)

Clinical Correlations

  • Vitamin A Deficiency: Epithelial tissues can be replaced with stratified squamous epithelium, particularly in the bronchi and bladder
  • Chronic Bronchitis: Smokers may exhibit squamous metaplasia, excessive mucus production, and airway obstruction
  • Gastric Ulcer: Helicobacter pylori can bind extracellular regions of tight junction proteins in stomach cells and disrupt the connection.

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