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Questions and Answers
What is a primary limitation of using heat-based paraffin wax infiltration for tissue processing?
What is a primary limitation of using heat-based paraffin wax infiltration for tissue processing?
Which of the following is NOT typically part of the initial steps of tissue processing?
Which of the following is NOT typically part of the initial steps of tissue processing?
What is the main purpose of using ascending concentrations of ethyl alcohol in tissue processing?
What is the main purpose of using ascending concentrations of ethyl alcohol in tissue processing?
What is the typical temperature range for an oven used in manual paraffin wax infiltration?
What is the typical temperature range for an oven used in manual paraffin wax infiltration?
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Which of the following is the most common clearing agent used in tissue processing?
Which of the following is the most common clearing agent used in tissue processing?
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What is a key advantage of using Carbowax in tissue processing?
What is a key advantage of using Carbowax in tissue processing?
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Which embedding medium is specially suitable for producing sections of eye specimens?
Which embedding medium is specially suitable for producing sections of eye specimens?
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Which characteristic of Ester wax differentiates it from paraffin wax?
Which characteristic of Ester wax differentiates it from paraffin wax?
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What is the purpose of the 'embedding' process in tissue preparation?
What is the purpose of the 'embedding' process in tissue preparation?
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What primary advantage does negative atmospheric pressure offer during the infiltration step?
What primary advantage does negative atmospheric pressure offer during the infiltration step?
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What is the primary purpose of infiltration in tissue processing?
What is the primary purpose of infiltration in tissue processing?
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Why is a firm consistency essential for tissue specimens after infiltration?
Why is a firm consistency essential for tissue specimens after infiltration?
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Which of the following is the most commonly used medium for infiltration?
Which of the following is the most commonly used medium for infiltration?
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At what temperature does the paraffin wax used for infiltration typically melt?
At what temperature does the paraffin wax used for infiltration typically melt?
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What does constant agitation accomplish in the infiltration process?
What does constant agitation accomplish in the infiltration process?
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What is the primary function of the autotechnicon in tissue processing?
What is the primary function of the autotechnicon in tissue processing?
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Which of the following methods uses negative atmospheric pressure inside an oven to speed up the paraffin wax infiltration?
Which of the following methods uses negative atmospheric pressure inside an oven to speed up the paraffin wax infiltration?
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How many changes of wax are typically needed during infiltration to ensure complete impregnation?
How many changes of wax are typically needed during infiltration to ensure complete impregnation?
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What is the primary purpose of using a float-out bath in histological procedures?
What is the primary purpose of using a float-out bath in histological procedures?
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Which material is LEAST advisable for stropping a knife, according to the text?
Which material is LEAST advisable for stropping a knife, according to the text?
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What is the recommended temperature range for a float out bath in degrees Celsius?
What is the recommended temperature range for a float out bath in degrees Celsius?
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What type of movement should be used when stropping a knife?
What type of movement should be used when stropping a knife?
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What is the process of 'fishing out' in the context of histology?
What is the process of 'fishing out' in the context of histology?
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Which material is most commonly utilized as an embedding medium?
Which material is most commonly utilized as an embedding medium?
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What is the recommended temperature for rapid cooling of paraffin during embedding?
What is the recommended temperature for rapid cooling of paraffin during embedding?
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What is a key advantage of using plastic embedding rings, such as Tissue-Tek?
What is a key advantage of using plastic embedding rings, such as Tissue-Tek?
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Which substance should be used to coat the mold for easy removal of the paraffin block?
Which substance should be used to coat the mold for easy removal of the paraffin block?
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What is the main purpose of using a compound embedding unit?
What is the main purpose of using a compound embedding unit?
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Which of these options is NOT a type of mold mentioned for embedding?
Which of these options is NOT a type of mold mentioned for embedding?
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What does the term 'infiltration' refer to in the context of embedding?
What does the term 'infiltration' refer to in the context of embedding?
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What is the primary function of formalin when used in gelatin infiltration?
What is the primary function of formalin when used in gelatin infiltration?
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Why is the orientation of the specimen considered easy when using plastic embedding rings or Bass molds?
Why is the orientation of the specimen considered easy when using plastic embedding rings or Bass molds?
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Besides a tissue softener in decalcification, what is another use for phenol?
Besides a tissue softener in decalcification, what is another use for phenol?
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What is the main purpose of the embedding process in tissue preparation?
What is the main purpose of the embedding process in tissue preparation?
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Which of the following describes the standard solution and time required for tissue immersion?
Which of the following describes the standard solution and time required for tissue immersion?
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What are the common embedding mediums used?
What are the common embedding mediums used?
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What characterizes Leuchhart's embedding molds?
What characterizes Leuchhart's embedding molds?
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What is the alternative name for 'embedding' in tissue processing?
What is the alternative name for 'embedding' in tissue processing?
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What is an advantage of using Leuchhart’s embedding molds that is mentioned in the content?
What is an advantage of using Leuchhart’s embedding molds that is mentioned in the content?
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Flashcards
Fixation
Fixation
The initial step in tissue processing that uses formaldehye to preserve cell structures and harden the tissue for easier cutting.
Dehydration
Dehydration
The process of gradually removing water from the tissue using increasing concentrations of alcohol, preparing it for paraffin wax.
Clearing
Clearing
The process of removing alcohol from the tissue using a clearing agent like xylene, making the tissue transparent for wax infiltration.
Infiltration
Infiltration
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Paraffin wax infiltration
Paraffin wax infiltration
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Paraffin Wax
Paraffin Wax
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Melting Point
Melting Point
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Autotechnicon
Autotechnicon
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Heat Assisted Infiltration
Heat Assisted Infiltration
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Vacuum Infiltration
Vacuum Infiltration
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Agitation
Agitation
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Multiple Changes of Wax
Multiple Changes of Wax
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Tissue Embedding
Tissue Embedding
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Ester Wax
Ester Wax
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Paraplast
Paraplast
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Carbowax
Carbowax
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Celloidin Infiltration
Celloidin Infiltration
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Stropping
Stropping
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Float out bath
Float out bath
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Fishing out
Fishing out
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Orientation
Orientation
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Mineral Oil
Mineral Oil
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Embedding
Embedding
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Embedding medium
Embedding medium
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Embedding mold
Embedding mold
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Leuchhart’s embedding molds
Leuchhart’s embedding molds
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Phenol in embedding
Phenol in embedding
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Gelatin embedding
Gelatin embedding
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Formalin in gelatin embedding
Formalin in gelatin embedding
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Casting/Blocking
Casting/Blocking
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Manual embedding
Manual embedding
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Interlocking plate mold
Interlocking plate mold
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Tissue-Tek plastic embedding rings
Tissue-Tek plastic embedding rings
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Bass molds
Bass molds
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Paper boats
Paper boats
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Study Notes
MTAP 2 - Infiltration to Embedding
- Histopathology specimens are processed similarly, regardless of type (autopsy, biopsy, surgical, cytology)
- Fixation preserves tissues to harden them for cutting
- Dehydration uses ascending ethanol concentrations
- Clearing uses xylene as a common agent
- Infiltration (impregnation) fills tissue spaces for consistent firmness for cutting
- Paraffin wax infiltration is the most common method, using an oven for a consistent temperature
Disadvantages of Paraffin Wax Infiltration
- Not suitable for fatty tissues due to melting
- Requires longer time for dense specimens
Methods of Paraffin Wax Infiltration
- Manual: Uses a dissecting pan with melted paraffin wax, placing the tissue in the pan and into an oven set 2–5°C above the wax's melting point. The process involves four 15-minute intervals over an hour.
- Automatic (Autotechnicon): An automatic tissue processor performs fixation, dehydration, clearing, and infiltration
- Requires less paraffin wax
- Rapid embedding
- Easy specimen orientation
Substitutes for Paraffin Wax
- Paraplast: For bones, brain specimens
- Bioloid/Embeddol: For eye specimens
- Carbowax: Water-soluble, eliminates clearing and dehydration, suitable for enzyme studies
Celloidin Infiltration
- No heat required; slow method
- Appropriate for specimens with significant cavities
- Requires 3 containers of increasing celloidin concentrations (2-4%, 4-6%, and 8-12%), immersing the specimens for varying durations
Gelatin Infiltration
- Recommended for eye specimens and enzyme studies
- Immerse the specimen in a gelatin solution for 24 hours (10% Gelatin + 1% phenol), then 12 hours (20% Gelatin + 1% phenol))
Embedding
- Places tissue into a mold with embedding medium.
- Paraffin wax is the most common embedding medium
- Produces tissue blocks suitable for cutting
Embedding Molds
- Disposable and non-disposable molds
- Hulmahan embedding molds
- Leuchhart's embedding molds (“L” pieces)
- Compound embedding unit
Types of Microtomes
- Ultrathin Microtome: For electron microscopy (0.5 µm thickness)
- Rotary Microtome: For paraffin embedded tissues (4-6 µm thickness)
- Rocking Microtome: (10-12 µm thickness)
- Sliding Microtome: (7–9 µm thickness)
- Base Sledge Microtome: Movable part is the block holder
- Cryostat: For immediately hardening fresh tissue using temperature
- Freezing Microtome: For tissues with heat-sensitive structures
- Diamond Knife: For electron microscopy
Types of Blades
- Biconcave: For paraffin tissue
- Plane Concave: Cut tough samples in paraffin
Honing and Stropping
- Honing: Removes nicks and irregularities from the blade
- Stropping: Polishes and sharpens the blade further
Paraffin Removal (Deparaffinization)
- Removes paraffin and clearing agents to ready samples for staining
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Description
Test your knowledge on the processes of histopathology specimens, focusing on paraffin wax infiltration methods and their advantages and disadvantages. This quiz will cover fixation, dehydration, clearing, and the techniques used in embedding tissues for microscopic evaluation.