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Questions and Answers
What is the primary mechanism by which MRSA develops resistance to methicillin and related antibiotics?
What is the primary mechanism by which MRSA develops resistance to methicillin and related antibiotics?
What initial symptom did the 14-year-old boy display before the onset of systemic symptoms?
What initial symptom did the 14-year-old boy display before the onset of systemic symptoms?
In which year was MRSA first discovered?
In which year was MRSA first discovered?
Which of the following antibiotics is MRSA resistant to?
Which of the following antibiotics is MRSA resistant to?
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Which population is most likely to acquire community-associated MRSA infections?
Which population is most likely to acquire community-associated MRSA infections?
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What is the primary characteristic of Staphylococcus species?
What is the primary characteristic of Staphylococcus species?
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Which of the following diseases is commonly associated with Staphylococcus aureus?
Which of the following diseases is commonly associated with Staphylococcus aureus?
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Which feature distinguishes methicillin-resistant Staphylococcus aureus (MRSA) from other strains?
Which feature distinguishes methicillin-resistant Staphylococcus aureus (MRSA) from other strains?
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What role do Staphylococcus species play in the human body?
What role do Staphylococcus species play in the human body?
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Which Staphylococcus species is most commonly linked to opportunistic infections of medical devices?
Which Staphylococcus species is most commonly linked to opportunistic infections of medical devices?
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Which of the following statements regarding Staphylococcus aureus is true?
Which of the following statements regarding Staphylococcus aureus is true?
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What type of oxygen requirement do Staphylococcus species have?
What type of oxygen requirement do Staphylococcus species have?
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Which of the following species is known to cause endocarditis?
Which of the following species is known to cause endocarditis?
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What is the main effect of the capsule produced by Staphylococcus aureus?
What is the main effect of the capsule produced by Staphylococcus aureus?
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Which of these is a virulence factor of Staphylococcus aureus that directly facilitates its spread in tissue?
Which of these is a virulence factor of Staphylococcus aureus that directly facilitates its spread in tissue?
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What role does Protein A play in the virulence of Staphylococcus aureus?
What role does Protein A play in the virulence of Staphylococcus aureus?
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What is the primary laboratory method used for identifying Staphylococcus aureus in a clinical specimen?
What is the primary laboratory method used for identifying Staphylococcus aureus in a clinical specimen?
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Which toxin produced by Staphylococcus aureus acts as a superantigen?
Which toxin produced by Staphylococcus aureus acts as a superantigen?
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What is the function of coagulase produced by Staphylococcus aureus?
What is the function of coagulase produced by Staphylococcus aureus?
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Which of the following toxins is associated with causing nausea and vomiting in Staphylococcus aureus infections?
Which of the following toxins is associated with causing nausea and vomiting in Staphylococcus aureus infections?
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In molecular detection methods for Staphylococcus aureus, which technique is commonly used?
In molecular detection methods for Staphylococcus aureus, which technique is commonly used?
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What type of agar media is used for inoculating clinical specimens of Staphylococcus aureus?
What type of agar media is used for inoculating clinical specimens of Staphylococcus aureus?
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What is the incubation temperature for Staphylococcus aureus during culture?
What is the incubation temperature for Staphylococcus aureus during culture?
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Which test distinguishes Staphylococcus aureus from other Staphylococcus species?
Which test distinguishes Staphylococcus aureus from other Staphylococcus species?
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What describes the typical colony appearance of Staphylococcus aureus after 24 hours of incubation?
What describes the typical colony appearance of Staphylococcus aureus after 24 hours of incubation?
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Which of the following tests is used to identify Staphylococcus aureus through latex agglutination?
Which of the following tests is used to identify Staphylococcus aureus through latex agglutination?
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Which of the following is NOT a disease caused by Staphylococcus aureus?
Which of the following is NOT a disease caused by Staphylococcus aureus?
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Which of the following correctly describes the Gram stain results for Staphylococcus aureus?
Which of the following correctly describes the Gram stain results for Staphylococcus aureus?
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What is the significance of the catalase test in identifying Staphylococcus species?
What is the significance of the catalase test in identifying Staphylococcus species?
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What are common characteristics of MRSA skin infections?
What are common characteristics of MRSA skin infections?
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Which of the following conditions is NOT caused by MRSA?
Which of the following conditions is NOT caused by MRSA?
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What is the drug of choice for intravenous therapy of MRSA?
What is the drug of choice for intravenous therapy of MRSA?
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Which statement about MRSA's antibiotic resistance is accurate?
Which statement about MRSA's antibiotic resistance is accurate?
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How many nosocomial infections are reported annually due to MRSA in the U.S. hospitals?
How many nosocomial infections are reported annually due to MRSA in the U.S. hospitals?
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Which alternative drugs are acceptable for treating non-life-threatening MRSA infections orally?
Which alternative drugs are acceptable for treating non-life-threatening MRSA infections orally?
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Which of the following correctly identifies a common inhabitant of human skin and mucous membranes?
Which of the following correctly identifies a common inhabitant of human skin and mucous membranes?
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What is the estimated annual expenditure in the U.S. associated with MRSA infections?
What is the estimated annual expenditure in the U.S. associated with MRSA infections?
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Flashcards
Staphylococcus
Staphylococcus
A genus of Gram-positive cocci often found in clusters, known for being part of the normal flora of humans.
S.aureus
S.aureus
The most virulent species of Staphylococcus, causing a wide range of diseases from skin infections to bacteremia.
S.epidermidis
S.epidermidis
A part of skin flora, it can cause opportunistic infections, especially in hospital settings.
S.saprophyticus
S.saprophyticus
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MRSA
MRSA
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Diseases caused by S.aureus
Diseases caused by S.aureus
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Normal flora locations
Normal flora locations
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Opportunistic infections
Opportunistic infections
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Acquisition of mec A and mec C
Acquisition of mec A and mec C
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Symptoms of MRSA infection
Symptoms of MRSA infection
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First discovery of MRSA
First discovery of MRSA
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Susceptible populations to MRSA
Susceptible populations to MRSA
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Staphylococcus aureus
Staphylococcus aureus
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S. aureus virulence factors
S. aureus virulence factors
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Capsule
Capsule
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Exfoliative toxins
Exfoliative toxins
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Toxic shock syndrome toxin-1 (TSST-1)
Toxic shock syndrome toxin-1 (TSST-1)
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Coagulase
Coagulase
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Culture and Gram Stain
Culture and Gram Stain
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MRSA skin infections
MRSA skin infections
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MRSA complications
MRSA complications
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MRSA prevalence in hospitals
MRSA prevalence in hospitals
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MRSA treatment resistance
MRSA treatment resistance
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Primary MRSA treatment
Primary MRSA treatment
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Alternatives to Vancomycin
Alternatives to Vancomycin
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Coagulase-Negative Staphylococci (CoNS)
Coagulase-Negative Staphylococci (CoNS)
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S. aureus culture media
S. aureus culture media
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Incubation temperature
Incubation temperature
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S. aureus colony appearance
S. aureus colony appearance
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Hemolysis on sheep blood agar
Hemolysis on sheep blood agar
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Catalase test importance
Catalase test importance
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Coagulase test purpose
Coagulase test purpose
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Rapid Latex Agglutination test
Rapid Latex Agglutination test
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Study Notes
Staphylococcus Overview
- Staphylococcus is a gram-positive cocci bacteria
- They appear in clusters, but can also be single cells (0.5-1.5 µm)
- Staphylococcus species are facultative anaerobes (meaning they can survive with or without oxygen)
- They are non-motile and non-spore forming
- Catalase positive
- There are ~80 species and subspecies of Staphylococcus
Staphylococcus Objectives
- Describe the classification of Staphylococcus and the key attributes of S. aureus, S. epidermidis, and S. saprophyticus
- Link Staphylococcus species to the illnesses they cause
- Identify places in the human body where Staphylococcus commonly resides
- Explain the significance of methicillin-resistant Staphylococcus aureus (MRSA)
Clinically Important Staphylococcus Species
- S. aureus is the most virulent
- S. epidermidis
- S. saprophyticus
- MRSA is a causative agent of serious infections in hospitalized individuals and those outside of hospitals, especially in previously healthy children and adults
Common Staphylococcus Species & Diseases
- S. aureus:
- Toxin-mediated diseases (e.g., food poisoning, scalded skin syndrome, toxic shock syndrome)
- Cutaneous infections (e.g., carbuncles, folliculitis, furuncles, impetigo, wound infections)
- Other infections (e.g., bacteremia, endocarditis, pneumonia, empyema, osteomyelitis, septic arthritis)
- S. epidermidis:
- Bacteremia
- Endocarditis
- Surgical wounds
- Opportunistic infections of catheters, shunts, and prosthetic devices
- S. lugdunensis:
- Endocarditis
- S. saprophyticus:
- Urinary tract infections
Staphylococcus aureus: Biology
- Part of normal microbiome of skin, mucous membranes, and feces
- S. aureus is the species most often associated with serious infections
Staphylococcus aureus: Virulence Factors
- Structural Components:
- Capsule: Inhibits chemotaxis and phagocytosis
- Slime layer: Facilitates adherence to foreign bodies
- Protein A: Inhibits antibody-mediated clearance
- Toxins:
- Cytotoxins: toxic to several cells
- Exfoliative toxins (ETA, ETB): Increase intestinal peristalsis and fluid loss
- Enterotoxins: Increase intestinal symptoms
- Toxic shock syndrome toxin-1: Superantigen, triggers significant immune response that can be harmful
- Enzymes:
- Coagulase: Converts fibrinogen to fibrin
- Hyaluronidase: Hydrolyzes hyaluronic acid
- Lipases: Hydrolyze lipids
- Nucleases: Hydrolyze DNA
Staphylococcus aureus: Laboratory Testing
- Specimen selection is critical for diagnosing S. aureus
- Specimen type depends on the site of infection
- Methods include:
- Culture
- Gram Stain
- Molecular detection (e.g., PCR) for S. aureus and MRSA
Staphylococcus aureus: Culture
- Inoculate clinical specimens onto a sheep blood agar that is well supplemented.
- Incubate at 37° Celsius in an aerobic environment
- S. aureus grows as large, smooth colonies within 24 hours
- Colonies often turn yellow (aureus or gold)
- S. aureus usually results in distinct hemolysis on most sheep blood agars
Staphylococcus Identification (cont.)
- Gram staining: Gram-positive cocci in clusters.
- Catalase test: Staphylococci are positive; streptococci are negative
- Coagulase test: S. aureus is positive; other staphylococcus species are negative
- Rapid Latex Agglutination test is used to identify S. aureus and methicillin-resistant S. aureus (MRSA), coagulase and/or protein A
Diseases caused by Staphylococcus aureus
- Skin and soft tissue infections
- Osteomyelitis
- Pneumonia
- Food poisoning
- Gastroenteritis
- Toxic shock syndrome
- Bacteremia
- Endocarditis
- Other infections (e.g., pneumonia, empyema, osteomyelitis, septic arthritis)
Toxin factors of Staphylococcus aureus for intracellular survival
- α-toxin: Pore-forming toxin that lyses cell membranes
- β-toxin: Hydrolyzes sphingomyelin
- PSMα: Helps escape from phagosomes
- Leukocidins (D, E, M): Kill leukocytes
S. aureus: Disseminated Diseases (Toxins)
- Scalded skin syndrome (in infants)
- Food poisoning (via enterotoxins)
- Toxic shock syndrome (multisystem intoxication)
S. aureus: Localized Diseases (Suppurative Infections)
- Impetigo (localized cutaneous infection)
- Folliculitis (impetigo involving hair follicles)
- Furuncles/Boils (pus filled cutaneous nodules)
- Carbuncles (coalescence of furuncles)
- Other infections (e.g. pneumonia, empyema, osteomyelitis, septic arthritis)
Bacteremia and Endocarditis
- Spread of bacteria into blood from an infection site
- Characterized by damage to the endothelial lining of the heart
- Can result from certain wound and soft tissue infections
Methicillin-Resistant S. aureus (MRSA)
- First discovered in 1961 in England
- First case in the US in the 1980s
- Bacteria now resistant to methicillin, amoxicillin, penicillin, oxacillin, and other common antibiotics
- Bacterial resistance stems from acquisition/gain of a gene (mec A and mec C) that encodes a penicillin-binding protein with low affinity for penicillin
- MRSA may cause various infections such as:
- Bacteremia
- Pneumonia
- Surgical site infections
- Endocarditis
- Bone and joint infections
Coagulase-Negative Staphylococci
- Common inhabitants of human skin and mucous membranes
- S. epidermidis is frequently observed in infections, followed by S. hominis, S. haemolyticus, and S. capitis
- S. epidermidis is a part of the flora of the new-born nasal mucosa and umbilicus.
- Causes various infections, incl. wound infections, urinary tract infections, catheter/shunt infections and prosthetic device infections
Staphylococcus epidermidis - the "accidental" pathogen
- Normal microbiome of skin.
- Catalase positive.
- Coagulase negative staphylococci.
- Low virulence.
- Disease includes: Endocarditis, prosthetics, IV infections
- Can cause a significant amount of nosocomial drain-related cases of meningitis
Staphylococcus saprophyticus
- Gram-positive, catalase positive, coagulase negative, non-hemolytic
- Disease: Second most common cause of UTIs in young and sexually active women
- Resistant to novobiocin
- Nitrofurantoin (Macrobid) or Trimethoprim-sulfamethoxazole (Bactrim) are common treatment.
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Description
Test your knowledge on Methicillin-resistant Staphylococcus aureus (MRSA) and the characteristics of Staphylococcus species. This quiz covers the mechanisms of resistance, symptoms, and associated infections relevant to MRSA. Perfect for students studying microbiology or infectious diseases.