mRNA Processing Techniques Quiz
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Questions and Answers

What is the function of 5' capping?

  • Aids in mRNA degradation
  • Facilitates mRNA transport out of the nucleus
  • Stabilizes the mRNA (correct)
  • Initiates transcription

    • What is the linkage between the cap and the mRNA molecule?

  • 5'→ 3' phosphodiester linkage
  • 3'→ 5' phosphodiester linkage
  • 5'→ 5' triphosphate linkage (correct)
  • 3'→ 3' phosphodiester linkage

    • What is the enzyme responsible for adding the GMP during 5' capping?

  • RNA polymerase
  • Guanine-7-methyltransferase
  • Guanylyltransferase (correct)
  • Polyadenylate polymerase

    • What is the enzyme responsible for catalyzing cap methylation during 5' capping?

    <p>Guanine-7-methyltransferase</p> Signup and view all the answers

    What is the sequence that marks the end of hn-mRNA during polyadenylation?

    <p>AAUAAA</p> Signup and view all the answers

    What is the substrate used by Polyadenylate polymerase during addition of the poly-A tail?

    <p>ATP</p> Signup and view all the answers

    What is the function of the poly-A tail?

    <p>Facilitates mRNA transport out of the nucleus</p> Signup and view all the answers

    How many adenine nucleotides are added to the 3' end during polyadenylation?

    <p>150-200</p> Signup and view all the answers

    Is the poly A tail sequence transcribed from the DNA?

    <p>No</p> Signup and view all the answers

    What is the enzyme responsible for catalyzing addition of the poly-A tail?

    <p>Polyadenylate polymerase</p> Signup and view all the answers

    What is the purpose of 5' capping and polyadenylation?

    <p>To stabilize and protect mRNA</p> Signup and view all the answers

    What is the purpose of cap methylation during 5' capping?

    <p>To stabilize and protect mRNA</p> Signup and view all the answers

    What is the function of 5' capping?

    <p>Stabilizes the mRNA</p> Signup and view all the answers

    What is the linkage between the cap and the mRNA molecule?

    <p>5'→ 5' triphosphate linkage</p> Signup and view all the answers

    What is the enzyme responsible for adding the GMP during 5' capping?

    <p>Guanylyltransferase</p> Signup and view all the answers

    What is the enzyme responsible for catalyzing cap methylation during 5' capping?

    <p>Guanine-7-methyltransferase</p> Signup and view all the answers

    What is the sequence that marks the end of hn-mRNA during polyadenylation?

    <p>AAUAAA</p> Signup and view all the answers

    What is the substrate used by Polyadenylate polymerase during addition of the poly-A tail?

    <p>ATP</p> Signup and view all the answers

    What is the function of the poly-A tail?

    <p>Facilitates mRNA transport out of the nucleus</p> Signup and view all the answers

    How many adenine nucleotides are added to the 3' end during polyadenylation?

    <p>150-200</p> Signup and view all the answers

    Is the poly A tail sequence transcribed from the DNA?

    <p>No</p> Signup and view all the answers

    What is the enzyme responsible for catalyzing addition of the poly-A tail?

    <p>Polyadenylate polymerase</p> Signup and view all the answers

    What is the purpose of 5' capping and polyadenylation?

    <p>To stabilize and protect mRNA</p> Signup and view all the answers

    What is the purpose of cap methylation during 5' capping?

    <p>To stabilize and protect mRNA</p> Signup and view all the answers

    What is the 5' cap made of?

    <p>A 7-methylguanosine</p> Signup and view all the answers

    What enzyme catalyzes the addition of the 5' cap?

    <p>Guanylyltransferase</p> Signup and view all the answers

    What is the function of the 5' cap?

    <p>Aids in translation</p> Signup and view all the answers

    What is the polyadenylation signal sequence?

    <p>The cleavage site for hn-mRNA</p> Signup and view all the answers

    How many adenine nucleotides are added to the 3' end during polyadenylation?

    <p>40-200</p> Signup and view all the answers

    What enzyme catalyzes the addition of the poly A tail?

    <p>Polyadenylate polymerase</p> Signup and view all the answers

    What substrate is used during polyadenylation?

    <p>ATP</p> Signup and view all the answers

    Is the poly A tail sequence transcribed from the DNA?

    <p>No</p> Signup and view all the answers

    What is the function of the poly A tail?

    <p>Stabilizes the mRNA</p> Signup and view all the answers

    What is the role of Guanine-7-methyltransferase?

    <p>Catalyzes the cap methylation</p> Signup and view all the answers

    What is the linkage of the 5' cap?

    <p>5'→ 5' triphosphate linkage</p> Signup and view all the answers

    What is the role of hn-mRNA?

    <p>Is the precursor to mRNA</p> Signup and view all the answers

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