mRNA Processing Techniques Quiz
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Questions and Answers

What is the function of 5' capping?

  • Aids in mRNA degradation
  • Facilitates mRNA transport out of the nucleus
  • Stabilizes the mRNA (correct)
  • Initiates transcription

What is the linkage between the cap and the mRNA molecule?

  • 5'→ 3' phosphodiester linkage
  • 3'→ 5' phosphodiester linkage
  • 5'→ 5' triphosphate linkage (correct)
  • 3'→ 3' phosphodiester linkage

What is the enzyme responsible for adding the GMP during 5' capping?

  • RNA polymerase
  • Guanine-7-methyltransferase
  • Guanylyltransferase (correct)
  • Polyadenylate polymerase

What is the enzyme responsible for catalyzing cap methylation during 5' capping?

<p>Guanine-7-methyltransferase (B)</p> Signup and view all the answers

What is the sequence that marks the end of hn-mRNA during polyadenylation?

<p>AAUAAA (D)</p> Signup and view all the answers

What is the substrate used by Polyadenylate polymerase during addition of the poly-A tail?

<p>ATP (A)</p> Signup and view all the answers

What is the function of the poly-A tail?

<p>Facilitates mRNA transport out of the nucleus (A)</p> Signup and view all the answers

How many adenine nucleotides are added to the 3' end during polyadenylation?

<p>150-200 (C)</p> Signup and view all the answers

Is the poly A tail sequence transcribed from the DNA?

<p>No (B)</p> Signup and view all the answers

What is the enzyme responsible for catalyzing addition of the poly-A tail?

<p>Polyadenylate polymerase (B)</p> Signup and view all the answers

What is the purpose of 5' capping and polyadenylation?

<p>To stabilize and protect mRNA (B)</p> Signup and view all the answers

What is the purpose of cap methylation during 5' capping?

<p>To stabilize and protect mRNA (C)</p> Signup and view all the answers

What is the function of 5' capping?

<p>Stabilizes the mRNA (A)</p> Signup and view all the answers

What is the linkage between the cap and the mRNA molecule?

<p>5'→ 5' triphosphate linkage (D)</p> Signup and view all the answers

What is the enzyme responsible for adding the GMP during 5' capping?

<p>Guanylyltransferase (C)</p> Signup and view all the answers

What is the enzyme responsible for catalyzing cap methylation during 5' capping?

<p>Guanine-7-methyltransferase (A)</p> Signup and view all the answers

What is the sequence that marks the end of hn-mRNA during polyadenylation?

<p>AAUAAA (C)</p> Signup and view all the answers

What is the substrate used by Polyadenylate polymerase during addition of the poly-A tail?

<p>ATP (D)</p> Signup and view all the answers

What is the function of the poly-A tail?

<p>Facilitates mRNA transport out of the nucleus (D)</p> Signup and view all the answers

How many adenine nucleotides are added to the 3' end during polyadenylation?

<p>150-200 (D)</p> Signup and view all the answers

Is the poly A tail sequence transcribed from the DNA?

<p>No (A)</p> Signup and view all the answers

What is the enzyme responsible for catalyzing addition of the poly-A tail?

<p>Polyadenylate polymerase (B)</p> Signup and view all the answers

What is the purpose of 5' capping and polyadenylation?

<p>To stabilize and protect mRNA (D)</p> Signup and view all the answers

What is the purpose of cap methylation during 5' capping?

<p>To stabilize and protect mRNA (B)</p> Signup and view all the answers

What is the 5' cap made of?

<p>A 7-methylguanosine (A)</p> Signup and view all the answers

What enzyme catalyzes the addition of the 5' cap?

<p>Guanylyltransferase (C)</p> Signup and view all the answers

What is the function of the 5' cap?

<p>Aids in translation (A)</p> Signup and view all the answers

What is the polyadenylation signal sequence?

<p>The cleavage site for hn-mRNA (A)</p> Signup and view all the answers

How many adenine nucleotides are added to the 3' end during polyadenylation?

<p>40-200 (A)</p> Signup and view all the answers

What enzyme catalyzes the addition of the poly A tail?

<p>Polyadenylate polymerase (A)</p> Signup and view all the answers

What substrate is used during polyadenylation?

<p>ATP (B)</p> Signup and view all the answers

Is the poly A tail sequence transcribed from the DNA?

<p>No (C)</p> Signup and view all the answers

What is the function of the poly A tail?

<p>Stabilizes the mRNA (C)</p> Signup and view all the answers

What is the role of Guanine-7-methyltransferase?

<p>Catalyzes the cap methylation (A)</p> Signup and view all the answers

What is the linkage of the 5' cap?

<p>5'→ 5' triphosphate linkage (A)</p> Signup and view all the answers

What is the role of hn-mRNA?

<p>Is the precursor to mRNA (D)</p> Signup and view all the answers

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