mRNA Processing and Transport
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Questions and Answers

Which type of RNA carries the amino acid to the translation machinery?

  • mRNA
  • microRNA
  • rRNA
  • tRNA (correct)

Which type of RNA is the most stable and makes up most of the ribosome?

  • rRNA (correct)
  • microRNA
  • tRNA
  • mRNA

Which type of RNA is rapidly degraded and necessary for regulating enzyme production?

  • tRNA
  • mRNA (correct)
  • microRNA
  • rRNA

Which type of RNA can have enzymatic activity and act as an enzyme to speed up reactions?

<p>rRNA (B)</p> Signup and view all the answers

Which type of RNA can interact with itself due to its single-stranded nature?

<p>tRNA (D)</p> Signup and view all the answers

What is the main difference between transcription and replication?

<p>Transcription copies the regulatory parts of DNA, while replication copies the building blocks of DNA. (D)</p> Signup and view all the answers

What is the function of the anticodon in tRNA?

<p>To bind to the mRNA in the ribosome (A)</p> Signup and view all the answers

What is the enzyme responsible for linking the correct amino acid to the correct tRNA?

<p>tRNA synthetase (A)</p> Signup and view all the answers

Which type of RNA is the most abundant in the cell?

<p>rRNA (C)</p> Signup and view all the answers

What is the term for the post-transcriptional modification of tRNA, where normal bases are modified?

<p>RNA modification (B)</p> Signup and view all the answers

Which process occurs after the formation of mature mRNA?

<p>Translation (B)</p> Signup and view all the answers

What is the purpose of post-transcriptional modifications?

<p>To process the mRNA for translation (C)</p> Signup and view all the answers

What is the direction of formation for the new strands in transcription?

<p>5 prime to 3 prime (B)</p> Signup and view all the answers

What is the function of RNA polymerase in transcription?

<p>To synthesize RNA from DNA template (A)</p> Signup and view all the answers

What is the main difference between DNA replication and transcription?

<p>Use of RNA polymerase instead of DNA polymerase (D)</p> Signup and view all the answers

What occurs during the initiation phase of transcription?

<p>RNA polymerase binds to the DNA promoter region (A)</p> Signup and view all the answers

What occurs during the elongation phase of transcription?

<p>RNA polymerase synthesizes RNA (C)</p> Signup and view all the answers

What occurs during the termination phase of transcription?

<p>RNA polymerase detaches from the DNA template (B)</p> Signup and view all the answers

What is the purpose of the 5 prime capping, splicing, and poly A tail formation?

<p>To make the mRNA mature and ready for translation (C)</p> Signup and view all the answers

What is the role of proteins in transcription?

<p>To assist in the initiation, elongation, and termination phases (B)</p> Signup and view all the answers

Which part of the cell is responsible for the production of R RNA?

<p>Nucleolus (D)</p> Signup and view all the answers

What is the role of the nucleolus in the production of ribosomes?

<p>Processing R RNA (D)</p> Signup and view all the answers

What is the effect of R RNA transcription on the structure of DNA during cell replication?

<p>DNA condenses (D)</p> Signup and view all the answers

What is the term for the uncondensed part of a chromosome where R RNA genes are located?

<p>Satellites (D)</p> Signup and view all the answers

What is the role of mRNA in protein synthesis?

<p>Bringing the message from DNA to ribosomes (C)</p> Signup and view all the answers

What is the starting point for RNA polymerase in transcription?

<p>Open region of DNA (C)</p> Signup and view all the answers

What are the cofactors required by RNA polymerase in transcription?

<p>Transcription factors (A)</p> Signup and view all the answers

What is the role of transcription factors in transcription?

<p>Regulating gene transcription (A)</p> Signup and view all the answers

What is the term for the region recognized by the sigma factor in prokaryotes?

<p>Minus 10 region (D)</p> Signup and view all the answers

What is the main difference between transcription in prokaryotes and eukaryotes?

<p>Number of transcription factors required (D)</p> Signup and view all the answers

Which enzyme catalyzes the reaction of attaching the correct amino acid to tRNA?

<p>Amino-acid tRNA synthetase (B)</p> Signup and view all the answers

In prokaryotes, what subunits form the 70S ribosome?

<p>50S and 30S (D)</p> Signup and view all the answers

What is the shape of the tRNA molecule when viewed in 3D?

<p>L-shaped (D)</p> Signup and view all the answers

Which type of RNA is mainly composed of ribosomal RNA (rRNA)?

<p>rRNA (A)</p> Signup and view all the answers

In eukaryotes, what are the subunits that form the ribosome?

<p>40S and 60S (C)</p> Signup and view all the answers

What is the purpose of post-transcriptional modifications of tRNA?

<p>To increase stability (D)</p> Signup and view all the answers

What is the position indicated by the 'S' in the 70S ribosome?

<p>Size (B)</p> Signup and view all the answers

Which type of RNA is made inside the nucleus?

<p>rRNA (D)</p> Signup and view all the answers

In prokaryotes, what are the subunits that form the ribosome?

<p>30S and 50S (B)</p> Signup and view all the answers

How many different amino-acid tRNA synthetases are there?

<p>20 (C)</p> Signup and view all the answers

Which of the following accurately describes the speed of RNA polymerases during transcription?

<p>RNA polymerases speed up the positioning of the RNA polymerase during transcription (B)</p> Signup and view all the answers

What is the role of RNA polymerase in transcription?

<p>RNA polymerase binds to the promoter region and initiates transcription (B)</p> Signup and view all the answers

What happens during the termination phase of transcription?

<p>RNA polymerase binds to the terminator region and terminates transcription (C)</p> Signup and view all the answers

What is the function of the poly A tail in post-transcriptional modifications?

<p>The poly A tail enhances the stability of the mRNA molecule (A)</p> Signup and view all the answers

What is the purpose of 5' prime capping in post-transcriptional modifications?

<p>5' prime capping enhances the stability of the mRNA molecule (A)</p> Signup and view all the answers

What is the purpose of splicing in post-transcriptional modifications?

<p>Splicing enhances the translation process (A)</p> Signup and view all the answers

Which of the following accurately describes the speed of RNA polymerases during transcription?

<p>RNA polymerases speed up the positioning of the RNA polymerase during transcription (D)</p> Signup and view all the answers

What is the main difference between transcription and replication?

<p>Transcription produces RNA molecules, while replication produces DNA molecules (C)</p> Signup and view all the answers

What is the purpose of post-transcriptional modifications?

<p>Post-transcriptional modifications enhance the stability of the mRNA molecule (C)</p> Signup and view all the answers

What occurs during the initiation phase of transcription?

<p>RNA polymerase recognizes and binds to the promoter region of the gene (D)</p> Signup and view all the answers

Which of the following is responsible for speeding up the positioning of RNA polymerase during transcription in prokaryotes?

<p>The sigma factor (D)</p> Signup and view all the answers

What is the purpose of the consensus sequence in prokaryotic transcription?

<p>To start transcription at the correct position (A)</p> Signup and view all the answers

Which region of the DNA is recognized by the sigma factor in prokaryotic transcription?

<p>The promoter region (A)</p> Signup and view all the answers

What is the role of the sigma factor in prokaryotic transcription?

<p>To bind the RNA polymerase to the DNA (D)</p> Signup and view all the answers

What is the function of the promoter region in prokaryotic transcription?

<p>To scan the DNA for the sigma factor (B)</p> Signup and view all the answers

What is the purpose of the consensus sequence in prokaryotic transcription?

<p>To start transcription at the correct position (B)</p> Signup and view all the answers

Which of the following is responsible for recognizing different consensus sequences and transcribing different genes in prokaryotes?

<p>The sigma factor (C)</p> Signup and view all the answers

What is the function of the promoter region in prokaryotic transcription?

<p>To scan the DNA for the sigma factor (C)</p> Signup and view all the answers

What is the role of the sigma factor in prokaryotic transcription?

<p>To bind the RNA polymerase to the DNA (C)</p> Signup and view all the answers

Which region of the DNA is recognized by the sigma factor in prokaryotic transcription?

<p>The promoter region (A)</p> Signup and view all the answers

Which term is often used interchangeably with 'sense strand' in the context of DNA replication?

<p>Minus strand (D)</p> Signup and view all the answers

What is the other name for the 'anti-sense strand' in the context of DNA replication?

<p>Plus strand (B)</p> Signup and view all the answers

Which of the following terms is NOT used interchangeably with 'anti-sense strand' in the context of DNA replication?

<p>Non-coding strand (D)</p> Signup and view all the answers

In prokaryotes, where does transcription take place?

<p>Cytoplasm (A)</p> Signup and view all the answers

What happens to the mRNA produced during transcription in prokaryotes?

<p>It is immediately available for translation (D)</p> Signup and view all the answers

Which of the following terms refers to the same thing as 'anti-sense strand', 'non-coding strand', and 'plus strand'?

<p>Template strand (B)</p> Signup and view all the answers

What is the main difference between transcription in prokaryotes and eukaryotes?

<p>Presence of a nucleus (C)</p> Signup and view all the answers

What is the main difference between transcription and replication?

<p>Direction of synthesis (B)</p> Signup and view all the answers

What is the purpose of the poly A tail in post-transcriptional modifications?

<p>To protect the mRNA from degradation (B)</p> Signup and view all the answers

Which cellular component is responsible for the production of rRNA?

<p>Nucleolus (A)</p> Signup and view all the answers

What is the function of the sigma factor in transcription?

<p>To find the consensus sequence and bind to the promoter (A)</p> Signup and view all the answers

What is the role of the RNA polymerase in transcription?

<p>To help the polymerase slide along the DNA and make RNA (B)</p> Signup and view all the answers

What is the speed at which RNA is produced during transcription?

<p>30 to 50 nucleotides per second (D)</p> Signup and view all the answers

What is the role of topoisomerases in transcription?

<p>To relieve tensions in the DNA molecule caused by supercoiling (C)</p> Signup and view all the answers

What is the ultimate RNA strand produced during transcription complementary to?

<p>The coding strand (B)</p> Signup and view all the answers

What is the term used to describe the non-template strand during transcription?

<p>The non-coding strand (B)</p> Signup and view all the answers

What is the term used to describe the non-template strand during transcription?

<p>The non-coding strand (D)</p> Signup and view all the answers

What is the main difference between DNA replication and transcription?

<p>DNA replication produces DNA molecules, while transcription produces RNA molecules (A)</p> Signup and view all the answers

What is the function of the poly A tail in post-transcriptional modifications?

<p>To protect the mRNA from degradation (C)</p> Signup and view all the answers

What is the purpose of splicing in post-transcriptional modifications?

<p>To remove introns and join exons (B)</p> Signup and view all the answers

Which of the following is the purpose of the five prime cap in mRNA?

<p>All of the above (D)</p> Signup and view all the answers

What is the main function of the poly-A tail in mRNA?

<p>To protect the mRNA against degradation (B)</p> Signup and view all the answers

Which of the following is true about the formation of the five prime cap in mRNA?

<p>All of the above (D)</p> Signup and view all the answers

What is the purpose of the termination phase in transcription?

<p>To stop RNA synthesis (D)</p> Signup and view all the answers

Which of the following is true about termination in prokaryotes?

<p>Termination is done by hairpin loops in the RNA (B)</p> Signup and view all the answers

What is the main difference between termination in prokaryotes and eukaryotes?

<p>Termination is more complex in eukaryotes (A)</p> Signup and view all the answers

What is the function of the five prime cap in mRNA transport?

<p>To facilitate proper transport of the mRNA (B)</p> Signup and view all the answers

What is the main purpose of splicing in mRNA processing?

<p>To remove introns from the mRNA (B)</p> Signup and view all the answers

What is the role of the poly-A tail in mRNA processing?

<p>To protect the mRNA against degradation (B)</p> Signup and view all the answers

What is the purpose of the guanin nucleotide in the formation of the five prime cap?

<p>To stabilize the RNA polymerase (B)</p> Signup and view all the answers

Which of the following is true about row dependent termination in transcription?

<p>The row factor recognizes a sequence coming out of the RNA polymerase and binds to it. (A)</p> Signup and view all the answers

What is the result of row factor binding to the RNA in row dependent termination?

<p>Destabilization of the RNA polymerase bound to the DNA. (B)</p> Signup and view all the answers

What are the two types of termination in transcription?

<p>Row independent and row dependent termination. (C)</p> Signup and view all the answers

What is the role of the row factor in transcription termination?

<p>To recognize a sequence coming out of the RNA polymerase and bind to it. (A)</p> Signup and view all the answers

What happens when the row factor binds to the RNA in transcription termination?

<p>The RNA polymerase bound to the DNA is destabilized and falls off. (D)</p> Signup and view all the answers

What is the result of row independent termination in transcription?

<p>The RNA polymerase reaches a termination sequence and stops transcription. (D)</p> Signup and view all the answers

What is the difference between row independent and row dependent termination?

<p>Row independent termination occurs when the RNA polymerase reaches a termination sequence, while row dependent termination involves the row factor binding to the RNA and destabilizing the RNA polymerase bound to the DNA. (D)</p> Signup and view all the answers

What is the role of the row factor in transcription?

<p>To recognize a sequence coming out of the RNA polymerase and bind to it. (A)</p> Signup and view all the answers

What are the two factors involved in transcription termination?

<p>Row factor and RNA polymerase. (D)</p> Signup and view all the answers

What is the result of row dependent termination in transcription?

<p>The RNA polymerase bound to the DNA is destabilized and falls off. (A)</p> Signup and view all the answers

What is the main difference between transcription in prokaryotes and eukaryotes?

<p>In prokaryotes, transcription and translation are simultaneous, while in eukaryotes, transcription and translation are separate processes. (B)</p> Signup and view all the answers

What is the purpose of post-transcriptional modifications of mRNA?

<p>To remove introns and splice together exons. (B)</p> Signup and view all the answers

What is the role of the ribosomes in protein synthesis?

<p>To catalyze the formation of peptide bonds between amino acids. (B)</p> Signup and view all the answers

What is the function of the promoter region in prokaryotic transcription?

<p>To provide a binding site for RNA polymerase. (B)</p> Signup and view all the answers

What is the term for the region recognized by the sigma factor in prokaryotes?

<p>Promoter region (A)</p> Signup and view all the answers

What is the starting point for RNA polymerase in transcription?

<p>Promoter region (B)</p> Signup and view all the answers

What occurs during the elongation phase of transcription?

<p>RNA polymerase synthesizes the mRNA molecule. (D)</p> Signup and view all the answers

Which type of RNA is made inside the nucleus?

<p>rRNA (B)</p> Signup and view all the answers

What is the other name for the 'anti-sense strand' in the context of DNA replication?

<p>Template strand (A)</p> Signup and view all the answers

What is the main difference between transcription and replication?

<p>Transcription involves the synthesis of a complementary RNA strand, while replication involves the synthesis of a complementary DNA strand. (C)</p> Signup and view all the answers

Which enzyme is responsible for synthesizing the poly-A tail at the end of mRNA?

<p>Poly-A polymerase (C)</p> Signup and view all the answers

What is the main reason for adding a longer poly-A tail to mRNA?

<p>Improving stability of mRNA (A)</p> Signup and view all the answers

Which enzyme is responsible for removing introns during mRNA processing?

<p>Spliceosome (D)</p> Signup and view all the answers

What happens to the introns after they are removed during mRNA processing?

<p>They are degraded by exonucleases (C)</p> Signup and view all the answers

What is the function of the five prime cap on mRNA?

<p>Preventing degradation of mRNA (B)</p> Signup and view all the answers

What is the role of ribonucleoproteins in mRNA processing?

<p>Binding to intron sequences (A)</p> Signup and view all the answers

What happens to the RNA polymerase during mRNA processing?

<p>It falls off the DNA (D)</p> Signup and view all the answers

What is the purpose of splicing in mRNA processing?

<p>To remove introns (A)</p> Signup and view all the answers

What is the function of the loop formed by the mRNA during mRNA processing?

<p>Enhancing translation efficiency (A)</p> Signup and view all the answers

What happens to the introns that are removed during mRNA processing?

<p>They are degraded by exonucleases (B)</p> Signup and view all the answers

Which of the following is true about termination in transcription?

<p>Termination occurs when the RNA polymerase recognizes a specific sequence on the RNA. (A)</p> Signup and view all the answers

What is the role of exon duplicates in eukaryotic transcription termination?

<p>Exon duplicates recognize specific sequences on the RNA. (C)</p> Signup and view all the answers

What is the function of enzymes in the formation of the poly A tail?

<p>Enzymes recognize specific sequences on the RNA and form the poly A tail. (B)</p> Signup and view all the answers

What happens after the RNA is cut in half during the formation of the poly A tail?

<p>Another enzyme binds to the RNA and forms the poly A tail. (A)</p> Signup and view all the answers

What is the purpose of the poly A tail in mRNA?

<p>To stabilize the mRNA. (B)</p> Signup and view all the answers

What is the main difference between termination in prokaryotes and eukaryotes?

<p>Prokaryotes terminate transcription when the RNA polymerase recognizes a specific sequence on the DNA, while eukaryotes terminate when the RNA polymerase recognizes a specific sequence on the RNA. (C)</p> Signup and view all the answers

What is the result of row factor binding to the RNA in row dependent termination?

<p>The RNA polymerase falls off the DNA. (A)</p> Signup and view all the answers

What is the purpose of splicing in post-transcriptional modifications?

<p>To remove introns from the RNA. (B)</p> Signup and view all the answers

What is the purpose of the 5' prime cap in mRNA?

<p>To protect the mRNA from degradation. (B)</p> Signup and view all the answers

Which process occurs after the formation of mature mRNA?

<p>Translation. (C)</p> Signup and view all the answers

Which level of regulation controls the formation of proteins by determining whether or not a gene is transcribed?

<p>Transcriptional control (C)</p> Signup and view all the answers

Which process involves the degradation of RNA molecules, thereby regulating the formation of proteins?

<p>RNA processing control (B)</p> Signup and view all the answers

Which process involves the regulation of the movement of RNA molecules within a cell?

<p>Transport control (C)</p> Signup and view all the answers

Which process involves the regulation of the synthesis of proteins from mRNA molecules?

<p>Translation control (B)</p> Signup and view all the answers

Which level of regulation involves the decision of whether or not to make alternative splicing of RNA molecules?

<p>RNA processing control (B)</p> Signup and view all the answers

Which level of regulation determines the amount of mRNA produced, thus influencing protein formation?

<p>Transcriptional control (D)</p> Signup and view all the answers

Which level of regulation involves the degradation of mRNA molecules?

<p>RNA processing control (D)</p> Signup and view all the answers

Which level of regulation involves the movement of RNA molecules within a cell?

<p>Transport control (B)</p> Signup and view all the answers

Which level of regulation involves the synthesis of proteins from mRNA molecules?

<p>Translation control (C)</p> Signup and view all the answers

Which level of regulation involves the decision of whether or not to make alternative splicing of RNA molecules?

<p>RNA processing control (A)</p> Signup and view all the answers

What is the purpose of alternative splicing in gene expression?

<p>To increase the complexity of proteins produced from a single gene (B)</p> Signup and view all the answers

What is the approximate number of genes found in the human DNA?

<p>30 to 40,000 (B)</p> Signup and view all the answers

What is the function of the poly A tail in mature mRNA?

<p>To protect the mRNA from degradation (B)</p> Signup and view all the answers

What is the role of export factors in mRNA transport?

<p>They facilitate the transport of mRNA out of the nucleus (A)</p> Signup and view all the answers

What is the purpose of the five prime cap in mature mRNA?

<p>To protect the mRNA from degradation (C)</p> Signup and view all the answers

What is the significance of tissue-specific splicing variants?

<p>They allow for the production of different proteins in different tissues (B)</p> Signup and view all the answers

What is the function of introns in gene expression?

<p>To increase the complexity of proteins produced from a single gene (D)</p> Signup and view all the answers

What is the significance of the DS-CAN gene in fly genetics?

<p>It produces over 38,000 different proteins (D)</p> Signup and view all the answers

What is the main difference between prokaryotic and eukaryotic gene expression?

<p>The presence of introns in eukaryotic genes (A)</p> Signup and view all the answers

What is the role of proteins in mRNA transport?

<p>To assist in the transport of mRNA out of the nucleus (D)</p> Signup and view all the answers

What is the role of SNURPs in mRNA processing?

<p>They recognize and bind to specific sequences at the beginning and end of introns (D)</p> Signup and view all the answers

What happens when SNURPs bind at the beginning and end of an intron?

<p>They create a loop by bringing the two ends of the intron together (D)</p> Signup and view all the answers

What is the function of the spliceosome in mRNA processing?

<p>To remove introns and glue exons together (B)</p> Signup and view all the answers

What happens to the RNA from the intron during mRNA processing?

<p>It can be cut in pieces and reused (D)</p> Signup and view all the answers

What is the purpose of cutting off the intron during mRNA processing?

<p>To remove non-coding regions and join the coding regions (D)</p> Signup and view all the answers

What is the name of the complex formed by SNURPs, RNA, and proteins during mRNA processing?

<p>Spliceosome (C)</p> Signup and view all the answers

What happens to the SNURPs after mRNA processing?

<p>They can be reused (D)</p> Signup and view all the answers

What is the function of the spliceosome in mRNA processing?

<p>To remove introns and glue exons together (C)</p> Signup and view all the answers

What happens to the RNA from the intron during mRNA processing?

<p>It can be cut in pieces and reused (C)</p> Signup and view all the answers

What is the purpose of cutting off the intron during mRNA processing?

<p>To remove non-coding regions and join the coding regions (A)</p> Signup and view all the answers

What is the name of the complex formed by SNURPs, RNA, and proteins during mRNA processing?

<p>Spliceosome (A)</p> Signup and view all the answers

What happens to the SNURPs after mRNA processing?

<p>They can be reused (C)</p> Signup and view all the answers

Which molecule is believed to have played a role in the origin of life, according to the lecture by Gerhard Johan and Haftog?

<p>RNA (C)</p> Signup and view all the answers

What is the function of tRNA in protein synthesis?

<p>It carries the amino acids to the ribosome (B)</p> Signup and view all the answers

Are the satellites on the chromosomes the same as telomeres?

<p>No (B)</p> Signup and view all the answers

What is the Shine-Dalgano sequence involved in?

<p>Translation (A)</p> Signup and view all the answers

What is the role of activators in transcription?

<p>They help position the RNA polymerase (B)</p> Signup and view all the answers

Where are the ribosomal RNA genes located in a chromosome?

<p>In the secondary constrictions (B)</p> Signup and view all the answers

Do 70S ribosomes occur in the cytoplasm of eukaryotes?

<p>No (A)</p> Signup and view all the answers

What is the role of topoisomerase in fixing coils in DNA?

<p>It turns the DNA around and glues it back together (D)</p> Signup and view all the answers

Does prokaryotic mRNA contain a poly-A tail?

<p>No (A)</p> Signup and view all the answers

Which molecule is responsible for bringing the amino acids to the ribosome during translation?

<p>tRNA (B)</p> Signup and view all the answers

What are the three main ways in which the formation of proteins can be regulated?

<p>RNA processing control, protein transport, and protein activity (B)</p> Signup and view all the answers

What are the three post-transcriptional modifications mentioned in the text?

<p>Poly-A tail, 5-prime cap, and splicing (B)</p> Signup and view all the answers

What is the purpose of the poly-A tail in post-transcriptional modifications?

<p>To protect the mRNA from degradation (A)</p> Signup and view all the answers

What is the purpose of the 5-prime cap on mRNA?

<p>To protect the mRNA from degradation (B)</p> Signup and view all the answers

What is the main difference between transcription in prokaryotes and eukaryotes?

<p>Prokaryotes have introns in their mRNA, while eukaryotes do not (B)</p> Signup and view all the answers

What is the function of the spliceosome in mRNA processing?

<p>To remove introns from the mRNA (D)</p> Signup and view all the answers

What happens when SNURPs bind at the beginning and end of an intron?

<p>The intron is spliced out of the mRNA (B)</p> Signup and view all the answers

What is the function of the loop formed by the mRNA during mRNA processing?

<p>To protect the mRNA from degradation (A)</p> Signup and view all the answers

What is the term for the post-transcriptional modification of tRNA, where normal bases are modified?

<p>Methylation (C)</p> Signup and view all the answers

What is the role of transcription factors in transcription?

<p>To bind to the promoter region and initiate transcription (A)</p> Signup and view all the answers

Which cellular component is responsible for breaking down damaged proteins?

<p>Proteasome (C)</p> Signup and view all the answers

What is the function of topoisomerases in DNA replication?

<p>To remove super coils (B)</p> Signup and view all the answers

Why does a longer poly A tail result in more stability of RNA?

<p>It prevents exonucleases from reaching the coding sequence (B)</p> Signup and view all the answers

What is the role of the Kozak rules in translation?

<p>To initiate translation (C)</p> Signup and view all the answers

What is the primary function of the poly A tail in post-transcriptional modifications?

<p>To prevent exonucleases from degrading RNA (B)</p> Signup and view all the answers

What is the role of exonucleases in RNA degradation?

<p>To degrade RNA from the end (C)</p> Signup and view all the answers

What happens when there are too many super coils in DNA?

<p>DNA becomes less accessible for proteins (B)</p> Signup and view all the answers

What is the main function of SNURPs in mRNA processing?

<p>To facilitate splicing (D)</p> Signup and view all the answers

What is the primary role of export factors in mRNA transport?

<p>To transport mRNA from the nucleus to the cytoplasm (D)</p> Signup and view all the answers

What is the function of proteins in transcription?

<p>To assist in the transcription process (A)</p> Signup and view all the answers

Which polymerase is responsible for transcribing the mRNA in eukaryotes?

<p>Polymerase 2 (A)</p> Signup and view all the answers

What is the function of exonucleases during mRNA synthesis?

<p>They degrade the RNA from the beginning (B)</p> Signup and view all the answers

What is the purpose of the poly A tail in mRNA?

<p>To degrade the mRNA (C)</p> Signup and view all the answers

What is the difference between pre-mRNA and hnRNA?

<p>Pre-mRNA contains introns while hnRNA does not (C)</p> Signup and view all the answers

What is the role of transcription factors in mRNA production?

<p>To help polymerase 2 bind correctly (A)</p> Signup and view all the answers

What is the purpose of alternative splicing?

<p>To produce different proteins from the same gene (C)</p> Signup and view all the answers

What is the function of the invariable domain of tRNA?

<p>To bind to the ribosome (A)</p> Signup and view all the answers

Can a protein survive forever?

<p>No, proteins have a very short lifespan (A)</p> Signup and view all the answers

What is the main difference between transcription and replication?

<p>Transcription produces RNA while replication produces DNA (C)</p> Signup and view all the answers

What happens to the RNA from the intron during mRNA processing?

<p>It is degraded by exonucleases (C)</p> Signup and view all the answers

Which type of DNA does not have a poly A tail?

<p>Prokaryotic DNA (A)</p> Signup and view all the answers

What is the function of topoisomerase one?

<p>Makes a single strand break, turns the DNA around, and glues it back together (B)</p> Signup and view all the answers

What is the function of topoisomerase two?

<p>Makes a double strand break, turns one side, and puts it back together (C)</p> Signup and view all the answers

What is the function of the five prime cap?

<p>Prevents RNA degradation by exonucleases (A)</p> Signup and view all the answers

What is the role of exonucleases in DNA and RNA degradation?

<p>They degrade DNA and RNA from the ends (C)</p> Signup and view all the answers

What is the shape of the tRNA molecule when viewed in 3D?

<p>Cloverleaf (A)</p> Signup and view all the answers

What is the function of the promoter region in prokaryotic transcription?

<p>Binds transcription factors (C)</p> Signup and view all the answers

What is the function of activators in transcription?

<p>Enhance transcription (B)</p> Signup and view all the answers

What is the purpose of the guanine nucleotide in the formation of the five prime cap?

<p>To prevent RNA degradation (B)</p> Signup and view all the answers

What is the significance of the DS-CAN gene in fly genetics?

<p>It is not mentioned in the text (D)</p> Signup and view all the answers

Which enzyme is responsible for removing super coils in DNA?

<p>Topoisomerase (C)</p> Signup and view all the answers

Why is a longer poly A tail believed to increase the stability of mRNA?

<p>It delays the reach of exonucleases to the coding sequence (A)</p> Signup and view all the answers

Which nucleotides are required for RNA synthesis?

<p>ATP, GTP, CTP, UTP (C)</p> Signup and view all the answers

What is the function of satellite and secondary constriction in chromosomes?

<p>They are consequences of DNA compaction and do not have a specific function (A)</p> Signup and view all the answers

Why is ATP important for RNA synthesis?

<p>It provides energy for the formation of phosphodiester bonds (C)</p> Signup and view all the answers

Why are GTP, TTP, and CTP not used for RNA synthesis?

<p>They are only used for DNA synthesis (D)</p> Signup and view all the answers

What is the role of exonucleases in RNA degradation?

<p>They remove nucleotides from the end of RNA molecules (B)</p> Signup and view all the answers

Which nucleotide is not used for RNA synthesis?

<p>TTP (D)</p> Signup and view all the answers

What is the consequence of DNA compaction in chromosomes?

<p>Formation of satellite and secondary constriction (A)</p> Signup and view all the answers

Which enzyme is responsible for removing super coils in DNA?

<p>Topoisomerase (A)</p> Signup and view all the answers

Which part of the chromosome is referred to as a satellite?

<p>The part of the chromosome after the constriction (C)</p> Signup and view all the answers

What is the purpose of the five prime cap?

<p>To protect against exonucleases (D)</p> Signup and view all the answers

Which RNA component makes up the ribosome?

<p>rRNA (A)</p> Signup and view all the answers

In eukaryotes, where does transcription end?

<p>At the polyA tail (B)</p> Signup and view all the answers

What is the function of the sigma factor in prokaryotic transcription?

<p>To bind to the promoter region (D)</p> Signup and view all the answers

In prokaryotes, where does translation start?

<p>At the non-coding sequence before the Shindal Garno (A)</p> Signup and view all the answers

What is the role of topoisomerases in DNA?

<p>To fix coils in the DNA (C)</p> Signup and view all the answers

Why are the coding and non-coding DNA strands referred to as minus and plus strands?

<p>Because the coding strand is not used for making mRNA (A)</p> Signup and view all the answers

What is the purpose of the polyA tail in mRNA?

<p>To stabilize the mRNA (B)</p> Signup and view all the answers

What is the main difference between prokaryotic and eukaryotic transcription termination?

<p>Prokaryotes use exonucleases to degrade the RNA chain, while eukaryotes use polyA enzymes (C)</p> Signup and view all the answers

Which of the following is a method by which cells can get rid of proteins they no longer need?

<p>Breaking them down (D)</p> Signup and view all the answers

What is the purpose of a poly-A tail in mRNA?

<p>To stabilize the mRNA molecule (C)</p> Signup and view all the answers

What happens when a hairpin structure forms in the RNA during transcription?

<p>The RNA synthesis stops (A)</p> Signup and view all the answers

Why is a poly-A tail added to mRNA instead of another nitrogen base?

<p>To enhance stability (B)</p> Signup and view all the answers

What is the main role of topoisomerases in DNA replication?

<p>To unwind the DNA strands (D)</p> Signup and view all the answers

What is the primary function of the poly-A tail in post-transcriptional modifications?

<p>To stabilize the mRNA molecule (C)</p> Signup and view all the answers

What is the main difference between termination in prokaryotes and eukaryotes?

<p>The presence of a hairpin structure in eukaryotes (B)</p> Signup and view all the answers

What is the role of mRNA in protein synthesis?

<p>To carry the genetic information from DNA to the ribosomes (C)</p> Signup and view all the answers

What happens to the introns that are removed during mRNA processing?

<p>They are destroyed (B)</p> Signup and view all the answers

What is the main reason for adding a longer poly-A tail to mRNA?

<p>To enhance stability (B)</p> Signup and view all the answers

Which process destabilizes the binding of RNA polymerase to DNA during transcription termination?

<p>Single strand binding proteins (B)</p> Signup and view all the answers

Why is the zone where transcription of rRNA occurs denser compared to the rest of the nucleus?

<p>It undergoes more processing (B)</p> Signup and view all the answers

Are single strand binding proteins used during transcription or translation?

<p>Transcription (D)</p> Signup and view all the answers

How does the sigma factor recognize the template strand during transcription?

<p>By binding to a specific sequence (B)</p> Signup and view all the answers

Which of the following is the main function of the poly A tail in post-transcriptional modifications?

<p>To stabilize the mRNA molecule (C)</p> Signup and view all the answers

What is the role of exonucleases in RNA degradation?

<p>To break down RNA from the ends (B)</p> Signup and view all the answers

Are the satellites on the chromosomes the same as telomeres?

<p>No (A)</p> Signup and view all the answers

Which enzyme is responsible for removing introns during mRNA processing?

<p>Spliceosome (C)</p> Signup and view all the answers

What is the primary function of the poly A tail in post-transcriptional modifications?

<p>To protect the mRNA from degradation (B)</p> Signup and view all the answers

What is the purpose of the poly A tail in mRNA?

<p>To stabilize the mRNA molecule (B)</p> Signup and view all the answers

Which direction does RNA polymerase always read in?

<p>3 prime, 5 prime direction (B)</p> Signup and view all the answers

How does RNA polymerase know which template to use for synthesizing mRNA?

<p>It reads the strand in the 3 prime, 5 prime direction (D)</p> Signup and view all the answers

Does the sigma factor recognize the template or non-template strand?

<p>It does not recognize either strand (C)</p> Signup and view all the answers

Would a eukaryotic mRNA yield the same polypeptide chain if it was translated by a 70S prokaryotic ribosome?

<p>Yes, because the codons are the same in prokaryotes and eukaryotes (A)</p> Signup and view all the answers

What is the purpose of the polyA tail in mRNA?

<p>It helps in mRNA stability (A)</p> Signup and view all the answers

What is the main difference between prokaryotic and eukaryotic gene expression?

<p>Prokaryotes have introns, while eukaryotes do not (C)</p> Signup and view all the answers

Which enzyme catalyzes the reaction of attaching the correct amino acid to tRNA?

<p>Aminoacyl-tRNA synthetase (B)</p> Signup and view all the answers

What is the role of the sigma factor in prokaryotic transcription?

<p>It recognizes the template strand (B)</p> Signup and view all the answers

What are the two factors involved in transcription termination?

<p>Rho factor and RNA polymerase (B)</p> Signup and view all the answers

What is the function of the five prime cap in mRNA transport?

<p>It helps in mRNA transport (B)</p> Signup and view all the answers

Flashcards

What is tRNA?

Transfer RNA (tRNA) is a type of RNA molecule that carries amino acids to the ribosome during protein synthesis.

What is rRNA?

Ribosomal RNA (rRNA) is a type of RNA that is the most stable and makes up the majority of the ribosome.

What is mRNA?

Messenger RNA (mRNA) is a type of RNA that carries genetic information from DNA to the ribosome, where it is translated into protein.

What is a ribozyme?

Some RNA molecules can act as enzymes, catalyzing specific biochemical reactions.

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What is the anticodon?

The anticodon is a three-nucleotide sequence on tRNA that is complementary to a specific codon on mRNA.

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What is tRNA synthetase?

tRNA synthetase is an enzyme that specifically attaches the correct amino acid to its corresponding tRNA molecule.

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What is transcription?

Transcription is the process of copying genetic information from DNA to RNA.

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What is DNA replication?

Replication is the process of copying DNA to produce two identical DNA molecules.

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What are post-transcriptional modifications?

Post-transcriptional modifications are changes that are made to RNA molecules after they have been transcribed.

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What is translation?

Translation is the process of converting the genetic code from mRNA into a protein.

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What is the nucleolus?

The nucleolus is a structure within the nucleus of a cell where ribosomal RNA (rRNA) is synthesized and ribosomes are assembled.

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What is the minus 10 region?

The minus 10 region is a specific sequence of DNA that is recognized by the sigma factor in prokaryotes, marking the starting point for transcription.

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What are transcription factors?

Transcription factors are proteins that bind to DNA and regulate the rate of transcription by controlling the activity of RNA polymerase.

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What is the 5' cap?

The 5' cap is a modified guanine nucleotide added to the 5' end of a pre-mRNA molecule during post-transcriptional modification.

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What is splicing?

Splicing is a process in post-transcriptional modification where non-coding sequences (introns) are removed from a pre-mRNA molecule, leaving only the coding sequences (exons).

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What is the poly A tail?

The poly A tail is a string of adenine nucleotides added to the 3' end of a pre-mRNA molecule during post-transcriptional modification.

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What is the promoter?

The promoter is a region of DNA that serves as a binding site for RNA polymerase and other proteins, initiating transcription.

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What is the terminator?

The terminator is a region of DNA that signals the end of transcription.

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What is the sigma factor?

The sigma factor is a protein in prokaryotes that helps RNA polymerase bind to the promoter and initiate transcription.

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What is RNA polymerase?

RNA polymerase is an enzyme that uses DNA as a template to synthesize an RNA molecule.

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What does the 'S' in the 70S ribosome stand for?

The 'S' in the 70S ribosome stands for Svedberg unit, a measure of sedimentation rate.

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What is amino-acid tRNA synthetase?

Amino-acid tRNA synthetase is an enzyme that catalyzes the attachment of the correct amino acid to its corresponding tRNA molecule.

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Where is rRNA synthesized?

rRNA is synthesized in the nucleolus and transported to the cytoplasm, where it forms a ribosomal subunit.

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What are the subunits of the ribosome in prokaryotes?

The small subunit of the ribosome in prokaryotes is 30S, while the large subunit is 50S.

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How many amino-acid tRNA synthetases are there?

There are 20 different amino-acid tRNA synthetases, one for each of the 20 amino acids.

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How fast does RNA polymerase move during transcription?

The speed of RNA polymerases during transcription is not constant.

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