Molecular Plant Pathology Lecture 5
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Questions and Answers

Which of the following pathogens are known to produce pectinases?

  • Verticillium alboatrum
  • Fusarium oxysporum
  • N. haematococca
  • All of the above (correct)
  • Pectinases are primarily involved in breaking down cellulose in plant cell walls.

    False (B)

    What is the primary function of pectinases in plant pathogens?

    Pectinases break down pectin, a component of the plant cell wall, which allows pathogens to penetrate and spread within plant tissues.

    The pathogens ________ and ________ are known to produce pectinases.

    <p>Verticillium dahliae, Fusarium oxysporum</p> Signup and view all the answers

    Match the plant pathogens with their primary target cell wall component:

    <p>Verticillium alboatrum = Pectin Fusarium oxysporum = Pectin N. haematococca = Pectin Cellulomonas = Cellulose</p> Signup and view all the answers

    Which of the following statements is TRUE regarding the role of pectinases in pathogenesis?

    <p>Pectinases help pathogens penetrate and spread within plant tissues. (D)</p> Signup and view all the answers

    Hemicelluloses consist only of xylan, mannan, and xyloglucan.

    <p>False (B)</p> Signup and view all the answers

    Describe the role of pectin in plant cell walls.

    <p>Pectin acts as a glue-like substance that cements plant cells together, providing structural support and rigidity to the cell wall.</p> Signup and view all the answers

    Which of the following fungal pathogens use cutinase for degradation and penetration of cutin?

    <p>All of the above (D)</p> Signup and view all the answers

    Lipases are involved in the germination of some pathogens by degrading waxy layers.

    <p>True (A)</p> Signup and view all the answers

    What is the primary function of cutinases in fungal pathogens?

    <p>Cutinases degrade the cutin layer of plant surfaces, allowing pathogens to penetrate and infect the host.</p> Signup and view all the answers

    The active site of cutinases consists of a catalytic triad of Ser, Asp, and ______.

    <p>His</p> Signup and view all the answers

    Match the cell wall degrading enzymes with their corresponding substrates:

    <p>Cellulases = Cellulose Hemicellulases = Hemicellulose Pectinases = Pectin Ligninases = Lignin</p> Signup and view all the answers

    Give an example of a fungal pathogen that utilizes pectinases to degrade plant cell walls.

    <p>Botrytis cinerea</p> Signup and view all the answers

    Which of the following statements is NOT true about cell wall degrading enzymes (CWDEs) in fungal pathogens?

    <p>CWDEs are only produced by necrotrophic fungal pathogens. (E)</p> Signup and view all the answers

    Basidiomycetes typically have a higher abundance of cellulolytic enzymes compared to Ascomycetes.

    <p>True (A)</p> Signup and view all the answers

    What is the primary function of pectinases in the context of plant cell walls?

    <p>Pectinases break down pectin, a major component of the plant cell wall, facilitating the degradation and softening of plant tissues.</p> Signup and view all the answers

    Pectinases are enzymes produced solely by plants for their own metabolic processes.

    <p>False (B)</p> Signup and view all the answers

    Which of the following enzymes preferentially attacks heavily methyl-esterified pectin substrates?

    <p>Pectin lyase (A)</p> Signup and view all the answers

    The enzyme ____ cleaves the α-1,4-linkage between galacturonosyl residues in pectate, which is a form of ____.

    Signup and view all the answers

    What is the primary function of pectinases in plant cell walls?

    <p>Pectinases break down the pectin in plant cell walls, which helps to weaken the cell wall structure and allows pathogens to penetrate into the plant tissue.</p> Signup and view all the answers

    Match the pectin-degrading enzymes with their preferred substrates.

    <p>Pectin Lyase = Heavily methyl-esterified pectin Pectate Lyase = Pectate (homogalacturonan) Polygalacturonase = Pectin, pectinic acid, pectate Pectin methyl esterase = Pectin and pectinic acid, to form pectate</p> Signup and view all the answers

    Flashcards

    Fusarium wilt

    A disease in tomatoes that causes vascular discoloration.

    Cutinase

    An enzyme that helps fungi degrade cutin to penetrate plant surfaces.

    Surface layer degradation

    The process where enzymes break down plant protective layers for infection.

    Constitutive-type cutinases

    Cutinases that help fungi adhere to plant surfaces during infection.

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    Germ tube assembly

    A stage in fungal infection where structures form to penetrate the host.

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    Cell Wall Degrading Enzymes (CWDEs)

    Enzymes that degrade plant cell walls for fungal growth.

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    Major classes of cellulases

    Different types of enzymes that break down cellulose.

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    Exoglucanases

    Enzymes that break down cellulose from the ends of chains.

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    Primary plant cell wall

    The outermost layer of a plant cell made of cellulose and other polysaccharides.

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    Middle lamella

    The layer between plant cell walls that contains pectin and acts as a cementing substance.

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    Cell wall-degrading enzymes

    Enzymes that break down components of the plant cell wall, aiding pathogens in invasion.

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    Hemicellulose

    A group of polysaccharides in the cell wall, varying by plant species and including xyloglucans, xylans, and mannans.

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    Pectin

    A gel-forming polysaccharide found in the primary cell wall, mainly composed of D-galacturonic acid.

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    Homogalacturonan (HG)

    A type of pectin that forms a linear polymer, responsible for cell wall rigidity.

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    Vascular wilt pathogens

    Pathogens affecting vascular systems in plants, often utilizing more pectinases to invade.

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    Pectic enzymes

    Enzymes that degrade pectin, facilitating parasite infection in plants.

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    Pectinase

    An enzyme that breaks down pectin into smaller units.

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    Pectin lyase

    An enzyme cleaving α-1,4-linkages in methylated pectin.

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    Pectin methylesterase (PME)

    An enzyme that converts pectin into pectate by removing methyl groups.

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    Pectate lyase

    An enzyme that breaks down pectate by cleaving α-(1-4) linkages.

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    Polygalacturonase (PG)

    An enzyme that hydrolyzes α-1,4-glycosidic bonds in pectin.

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    Pectate degradation

    The breakdown of pectate resulting in plant disease symptoms.

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    Fusarium oxysporum

    A fungus causing symptoms in infected plants via pectate degrading enzymes.

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    Study Notes

    Molecular Plant Pathology: Lecture 5 - Biochemical Methods: Cell Wall Degrading Enzymes

    • Learning Outcomes: Students should be able to describe primary plant cell wall and middle lamella components, list cell wall-degrading enzymes used by plant pathogens, explain the role of these and pectic enzymes in pathogenesis, and give pathogen examples that use these enzymes.

    Plant Cell Wall Structure

    • Primary Cell Wall: Contains cellulose microfibrils, hemicellulose, and pectin as major components.
    • Middle Lamella: Contains pectin, acts as a glue between adjacent plant cells.

    Cell Wall-Degrading Enzymes

    • Types: Cellulases, hemicellulases, pectinases, ligninases, endo- and exo-acting enzymes, and proteases are used by plant pathogens.
    • Functions: Degrade plant cell wall components, facilitating pathogen entry and colonization. This enables the release of carbohydrates for pathogen growth and reproduction.

    Hemicellulose

    • Classification: Based on the main sugar in the polymer backbone: xylan (β-1,4-linked D-xylose), mannan (β-1,4-linked D-mannose), and xyloglucan (β-1,4-linked D-glucose).
    • Structure Variation: Hemicellulose structure varies by plant species.

    Pectin

    • Backbone: Primarily composed of alpha-1,4-linked D-galacturonic acid residues, which can be methyl-esterified or substituted with acetyl groups.
    • Classification: Pectins are categorized into homogalacturonan (HG), xylogalacturonan, and rhamnogalacturonan (RG-I or RG-II) based on their structure.
    • Role: HG is responsible for plant cell wall rigidity, while RG-I potentially influences cell wall plasticity.

    Pathogens and CWDEs

    • Specific Pathogens: Many vascular wilt and root pathogens (e.g., Verticillium alboatrum, Verticillium dahlia, N. haematococca, Fusarium oxysporum) often exhibit higher pectinase activity.
    • Effect on Vascular Bundle: Pathogens might block or collapse vascular bundles during disease development.

    Surface Layer Degradation

    • Cutinase: Involved in cutin degradation and penetration in some pathogens (e.g., Botrytis cinerea, Fusarium, Colletotrichum).
    • Cutin: Hydrolyzes the ester bonds of the plant polymer cutin.
    • Lipases: Used by some pathogens to degrade waxy layers, crucial for germination, exemplified by Sclerotinia sclerotiorum.

    Cutinase Mechanism

    • Active Site: Serine, aspartic acid, and histidine residues comprise the highly conserved catalytic triad of cutinases.
    • Fungal Spores: Recognising cutin monomers and initiating cutinases production is vital for spores in order to successfully infect plants.

    Cellulose Degradation

    • Cellulose: Composed of glucose linked by β-(1,4') glycosidic bonds. Degraded through sequential cleavage by different classes of enzymes, including endoglucanase, exoglucanase, and β-glucosidase.
    • Classes of Cellulases: Exoglucanases (cellulose 1,4-β-cellobiosidases) , endoglucanases, and β-glucosidases.

    Hemicellulose Hydrolysis

    • Fungi: Use xylanases to degrade the linear β-1,4-xylan polysaccharide into xylose.
    • Enzymes: Additional enzymes facilitate the process (e.g., arabino-furanosidases, acetylxylan esterases, ferulic acid esterases, and a-glucuronidases)

    Hydrolysis of Hemicellulose Backbone

    • Xylan, Xyloglucan, Mannan: Specific endoglucanases for xylan, β-1,4-endoglucanases for xyloglucan, and β-1,4-endomannanases for mannan.
    • Other Enzymes: Xylosidases, glucosidases, or mannosidases may complete the degradation process.

    Pectin Hydrolysis

    • PGs or Pectin Depolymerases (PG), pectin lyase and pectate lyase : Involved in cleaving a-1,4 glycosidic linkages between D-galacturonic acid, crucial for their degradation.

    Pectinases

    • Polygalacturonidases (PGs): Typically part of the GH28 family, they specifically hydrolyze a-1,4-glycosidic linkages within a-galacturonic acid chains.
    • Pectin Lyases: Preferentially hydrolyze heavily methyl-esterified substrates, optimal pH around 5.5.
    • Pectate Lyases: Favor lower degrees of esterification, have optimal pH around 8.5, and require calcium ions for activity.

    Pectate Degradation

    • Symptoms (Fusarium oxysporum): Cause wilting of shoots and leaves, chlorosis of vascular system, and decrease in transpiration in infected plants.

    Other Important Enzymes and Concepts

    • CAZymes: Carbohydrate-active enzymes, instrumental in degrading plant polysaccharides by fungi during infection, essential for pathogenicity.
    • PGIPs: Polygalacturonase-inhibiting proteins, produced by plants, which restrain microbial CWDEs in a defense mechanism.
    • Necrotrophs and CWDEs: Necrotic pathogens can display a broad host range due to their extensive CWDE repertoire.

    Pathogen Erwinia chrysanthemi

    • Wide Host Range: Causes tissue rots in many fruits and vegetables; high pectinase production enables widespread tissue maceration.
    • Operon Required for Pathogenicity: Operons encode critical pectin-degrading enzymes (PG, PMEs, PLs) for effective pathogenicity.

    Bacterial Infection of Plant Tubers

    • Spread Mechanisms: Bacteria from infected tubers can spread to new plants through various means (insects, soil, etc). Infection often through wounds.

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    Description

    This quiz covers biochemical methods related to plant pathology, focusing on cell wall degrading enzymes. Students will explore the components of the primary plant cell wall and middle lamella, as well as the role of various enzymes in pathogen interactions. Understanding these concepts is crucial for comprehending plant disease mechanisms.

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