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Questions and Answers
Which of the following pathogens are known to produce pectinases?
Which of the following pathogens are known to produce pectinases?
Pectinases are primarily involved in breaking down cellulose in plant cell walls.
Pectinases are primarily involved in breaking down cellulose in plant cell walls.
False (B)
What is the primary function of pectinases in plant pathogens?
What is the primary function of pectinases in plant pathogens?
Pectinases break down pectin, a component of the plant cell wall, which allows pathogens to penetrate and spread within plant tissues.
The pathogens ________ and ________ are known to produce pectinases.
The pathogens ________ and ________ are known to produce pectinases.
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Match the plant pathogens with their primary target cell wall component:
Match the plant pathogens with their primary target cell wall component:
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Which of the following statements is TRUE regarding the role of pectinases in pathogenesis?
Which of the following statements is TRUE regarding the role of pectinases in pathogenesis?
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Hemicelluloses consist only of xylan, mannan, and xyloglucan.
Hemicelluloses consist only of xylan, mannan, and xyloglucan.
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Describe the role of pectin in plant cell walls.
Describe the role of pectin in plant cell walls.
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Which of the following fungal pathogens use cutinase for degradation and penetration of cutin?
Which of the following fungal pathogens use cutinase for degradation and penetration of cutin?
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Lipases are involved in the germination of some pathogens by degrading waxy layers.
Lipases are involved in the germination of some pathogens by degrading waxy layers.
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What is the primary function of cutinases in fungal pathogens?
What is the primary function of cutinases in fungal pathogens?
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The active site of cutinases consists of a catalytic triad of Ser, Asp, and ______.
The active site of cutinases consists of a catalytic triad of Ser, Asp, and ______.
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Match the cell wall degrading enzymes with their corresponding substrates:
Match the cell wall degrading enzymes with their corresponding substrates:
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Give an example of a fungal pathogen that utilizes pectinases to degrade plant cell walls.
Give an example of a fungal pathogen that utilizes pectinases to degrade plant cell walls.
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Which of the following statements is NOT true about cell wall degrading enzymes (CWDEs) in fungal pathogens?
Which of the following statements is NOT true about cell wall degrading enzymes (CWDEs) in fungal pathogens?
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Basidiomycetes typically have a higher abundance of cellulolytic enzymes compared to Ascomycetes.
Basidiomycetes typically have a higher abundance of cellulolytic enzymes compared to Ascomycetes.
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What is the primary function of pectinases in the context of plant cell walls?
What is the primary function of pectinases in the context of plant cell walls?
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Pectinases are enzymes produced solely by plants for their own metabolic processes.
Pectinases are enzymes produced solely by plants for their own metabolic processes.
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Which of the following enzymes preferentially attacks heavily methyl-esterified pectin substrates?
Which of the following enzymes preferentially attacks heavily methyl-esterified pectin substrates?
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The enzyme ____ cleaves the α-1,4-linkage between galacturonosyl residues in pectate, which is a form of ____.
The enzyme ____ cleaves the α-1,4-linkage between galacturonosyl residues in pectate, which is a form of ____.
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What is the primary function of pectinases in plant cell walls?
What is the primary function of pectinases in plant cell walls?
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Match the pectin-degrading enzymes with their preferred substrates.
Match the pectin-degrading enzymes with their preferred substrates.
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Flashcards
Fusarium wilt
Fusarium wilt
A disease in tomatoes that causes vascular discoloration.
Cutinase
Cutinase
An enzyme that helps fungi degrade cutin to penetrate plant surfaces.
Surface layer degradation
Surface layer degradation
The process where enzymes break down plant protective layers for infection.
Constitutive-type cutinases
Constitutive-type cutinases
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Germ tube assembly
Germ tube assembly
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Cell Wall Degrading Enzymes (CWDEs)
Cell Wall Degrading Enzymes (CWDEs)
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Major classes of cellulases
Major classes of cellulases
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Exoglucanases
Exoglucanases
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Primary plant cell wall
Primary plant cell wall
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Middle lamella
Middle lamella
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Cell wall-degrading enzymes
Cell wall-degrading enzymes
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Hemicellulose
Hemicellulose
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Pectin
Pectin
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Homogalacturonan (HG)
Homogalacturonan (HG)
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Vascular wilt pathogens
Vascular wilt pathogens
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Pectic enzymes
Pectic enzymes
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Pectinase
Pectinase
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Pectin lyase
Pectin lyase
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Pectin methylesterase (PME)
Pectin methylesterase (PME)
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Pectate lyase
Pectate lyase
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Polygalacturonase (PG)
Polygalacturonase (PG)
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Pectate degradation
Pectate degradation
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Fusarium oxysporum
Fusarium oxysporum
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Study Notes
Molecular Plant Pathology: Lecture 5 - Biochemical Methods: Cell Wall Degrading Enzymes
- Learning Outcomes: Students should be able to describe primary plant cell wall and middle lamella components, list cell wall-degrading enzymes used by plant pathogens, explain the role of these and pectic enzymes in pathogenesis, and give pathogen examples that use these enzymes.
Plant Cell Wall Structure
- Primary Cell Wall: Contains cellulose microfibrils, hemicellulose, and pectin as major components.
- Middle Lamella: Contains pectin, acts as a glue between adjacent plant cells.
Cell Wall-Degrading Enzymes
- Types: Cellulases, hemicellulases, pectinases, ligninases, endo- and exo-acting enzymes, and proteases are used by plant pathogens.
- Functions: Degrade plant cell wall components, facilitating pathogen entry and colonization. This enables the release of carbohydrates for pathogen growth and reproduction.
Hemicellulose
- Classification: Based on the main sugar in the polymer backbone: xylan (β-1,4-linked D-xylose), mannan (β-1,4-linked D-mannose), and xyloglucan (β-1,4-linked D-glucose).
- Structure Variation: Hemicellulose structure varies by plant species.
Pectin
- Backbone: Primarily composed of alpha-1,4-linked D-galacturonic acid residues, which can be methyl-esterified or substituted with acetyl groups.
- Classification: Pectins are categorized into homogalacturonan (HG), xylogalacturonan, and rhamnogalacturonan (RG-I or RG-II) based on their structure.
- Role: HG is responsible for plant cell wall rigidity, while RG-I potentially influences cell wall plasticity.
Pathogens and CWDEs
- Specific Pathogens: Many vascular wilt and root pathogens (e.g., Verticillium alboatrum, Verticillium dahlia, N. haematococca, Fusarium oxysporum) often exhibit higher pectinase activity.
- Effect on Vascular Bundle: Pathogens might block or collapse vascular bundles during disease development.
Surface Layer Degradation
- Cutinase: Involved in cutin degradation and penetration in some pathogens (e.g., Botrytis cinerea, Fusarium, Colletotrichum).
- Cutin: Hydrolyzes the ester bonds of the plant polymer cutin.
- Lipases: Used by some pathogens to degrade waxy layers, crucial for germination, exemplified by Sclerotinia sclerotiorum.
Cutinase Mechanism
- Active Site: Serine, aspartic acid, and histidine residues comprise the highly conserved catalytic triad of cutinases.
- Fungal Spores: Recognising cutin monomers and initiating cutinases production is vital for spores in order to successfully infect plants.
Cellulose Degradation
- Cellulose: Composed of glucose linked by β-(1,4') glycosidic bonds. Degraded through sequential cleavage by different classes of enzymes, including endoglucanase, exoglucanase, and β-glucosidase.
- Classes of Cellulases: Exoglucanases (cellulose 1,4-β-cellobiosidases) , endoglucanases, and β-glucosidases.
Hemicellulose Hydrolysis
- Fungi: Use xylanases to degrade the linear β-1,4-xylan polysaccharide into xylose.
- Enzymes: Additional enzymes facilitate the process (e.g., arabino-furanosidases, acetylxylan esterases, ferulic acid esterases, and a-glucuronidases)
Hydrolysis of Hemicellulose Backbone
- Xylan, Xyloglucan, Mannan: Specific endoglucanases for xylan, β-1,4-endoglucanases for xyloglucan, and β-1,4-endomannanases for mannan.
- Other Enzymes: Xylosidases, glucosidases, or mannosidases may complete the degradation process.
Pectin Hydrolysis
- PGs or Pectin Depolymerases (PG), pectin lyase and pectate lyase : Involved in cleaving a-1,4 glycosidic linkages between D-galacturonic acid, crucial for their degradation.
Pectinases
- Polygalacturonidases (PGs): Typically part of the GH28 family, they specifically hydrolyze a-1,4-glycosidic linkages within a-galacturonic acid chains.
- Pectin Lyases: Preferentially hydrolyze heavily methyl-esterified substrates, optimal pH around 5.5.
- Pectate Lyases: Favor lower degrees of esterification, have optimal pH around 8.5, and require calcium ions for activity.
Pectate Degradation
- Symptoms (Fusarium oxysporum): Cause wilting of shoots and leaves, chlorosis of vascular system, and decrease in transpiration in infected plants.
Other Important Enzymes and Concepts
- CAZymes: Carbohydrate-active enzymes, instrumental in degrading plant polysaccharides by fungi during infection, essential for pathogenicity.
- PGIPs: Polygalacturonase-inhibiting proteins, produced by plants, which restrain microbial CWDEs in a defense mechanism.
- Necrotrophs and CWDEs: Necrotic pathogens can display a broad host range due to their extensive CWDE repertoire.
Pathogen Erwinia chrysanthemi
- Wide Host Range: Causes tissue rots in many fruits and vegetables; high pectinase production enables widespread tissue maceration.
- Operon Required for Pathogenicity: Operons encode critical pectin-degrading enzymes (PG, PMEs, PLs) for effective pathogenicity.
Bacterial Infection of Plant Tubers
- Spread Mechanisms: Bacteria from infected tubers can spread to new plants through various means (insects, soil, etc). Infection often through wounds.
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Description
This quiz covers biochemical methods related to plant pathology, focusing on cell wall degrading enzymes. Students will explore the components of the primary plant cell wall and middle lamella, as well as the role of various enzymes in pathogen interactions. Understanding these concepts is crucial for comprehending plant disease mechanisms.