Molecular Genetics and Control Systems Quiz

Prof. Wood teaches ______ 340: Introduction to Biomolecular ______ngineering Lecture 8: Molecular Genetics & Control Systems (Chapter 4 Shuler) I. Overview Discuss the synthesis of protein, DNA, RNA regulation of the systems which control cellular dynamics. recombinant engineering. II.Protein Synthesis in Procaryotes A. Review of Replication for each step: DNA + dNTP DNA-dNMP + PPi Where does ______ come from for polymerization: from the two phosphate bonds that are removed as PPi. DNA ds (A-T, G-C) RNA single-stranded (A-U, G-C) replication: synthesis of DNA catalyzed by DNA polymerase add nucleotides in 5' to 3' direction each new ds piece of DNA has one new & one old strand How is 2nd strand made. schematic of replication: Okazaki fragments formed, series of short pieces of DNA (1000-2000 nt long bacteria, 100-200 nt long in eucaryotes (Cell & p90 IMN)) bidirectional replication at each of two forks rate = 1000 bp/sec at each fork or 500 nt/sec for ea enzyme (p93 IMN) make 1 mistake (incorrect pairing.

Prof. Wood teaches ______ 340

Review of Replication for each step: DNA + dNTP DNA-dNMP + PPi Where does ______ come from for polymerization

DNA ds (A-T, G-C) RNA single-stranded (A-U, G-C) replication: synthesis of DNA catalyzed by DNA ______

Schematic of replication: Okazaki fragments formed, series of short pieces of DNA (1000-2000 nt long bacteria, 100-200 nt long in eucaryotes (Cell & p90 IMN)) bidirectional replication at each of two forks rate = 1000 bp/sec at each fork or 500 nt/sec for ea enzyme (p93 IMN) make 1 mistake (incorrect ______)

Protein Synthesis in Procaryotes A. ______ of Replication for each step