Questions and Answers
Which technique uses antibodies to detect specific proteins in a sample?
Which technique is used to amplify specific DNA sequences?
Which technique involves separating DNA fragments based on size?
Which technique determines the order of nucleotides in DNA?
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Which technique is used to join DNA fragments together?
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Which technique is used to generate DNA fragments for cloning and analysis?
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Which technique separates proteins based on size?
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Which technique identifies proteins based on their mass-to-charge ratio?
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Study Notes
Molecular Biology Techniques in Medical Biochemistry
DNA Manipulation Techniques
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Restriction Enzymes: enzymes that cut DNA at specific recognition sites
- Used to generate DNA fragments for cloning and analysis
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DNA Ligase: enzyme that seals gaps in DNA
- Used to join DNA fragments together
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PCR (Polymerase Chain Reaction): amplifies specific DNA sequences
- Used to generate multiple copies of DNA for analysis or cloning
DNA Analysis Techniques
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Agarose Gel Electrophoresis: separates DNA fragments based on size
- Used to analyze DNA fragment lengths and detect genetic mutations
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Southern Blot: transfers DNA fragments from gel to membrane for hybridization
- Used to detect specific DNA sequences
-
DNA Sequencing: determines the order of nucleotides in DNA
- Used to identify genetic mutations and diagnose genetic diseases
Protein Analysis Techniques
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SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis): separates proteins based on size
- Used to analyze protein structure and identify protein biomarkers
-
Western Blot: transfers proteins from gel to membrane for antibody detection
- Used to detect specific proteins and diagnose diseases
-
Mass Spectrometry: identifies proteins based on mass-to-charge ratio
- Used to analyze protein structure and identify protein biomarkers
Genomics and Proteomics
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Microarray Analysis: analyzes gene expression levels on a large scale
- Used to identify genes involved in disease pathways
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Next-Generation Sequencing (NGS): analyzes entire genomes or transcriptomes
- Used to identify genetic mutations and diagnose genetic diseases
-
Protein Microarrays: analyzes protein-protein interactions on a large scale
- Used to identify protein biomarkers and understand disease mechanisms
Molecular Biology Techniques in Medical Biochemistry
DNA Manipulation Techniques
- Restriction enzymes cut DNA at specific recognition sites to generate fragments for cloning and analysis.
- DNA ligase seals gaps in DNA to join fragments together.
- PCR (Polymerase Chain Reaction) amplifies specific DNA sequences to generate multiple copies for analysis or cloning.
DNA Analysis Techniques
- Agarose gel electrophoresis separates DNA fragments based on size to analyze fragment lengths and detect genetic mutations.
- Southern blot transfers DNA fragments from gel to membrane for hybridization to detect specific DNA sequences.
- DNA sequencing determines the order of nucleotides in DNA to identify genetic mutations and diagnose genetic diseases.
Protein Analysis Techniques
- SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) separates proteins based on size to analyze protein structure and identify protein biomarkers.
- Western blot transfers proteins from gel to membrane for antibody detection to diagnose diseases.
- Mass spectrometry identifies proteins based on mass-to-charge ratio to analyze protein structure and identify protein biomarkers.
Genomics and Proteomics
- Microarray analysis examines gene expression levels on a large scale to identify genes involved in disease pathways.
- Next-Generation Sequencing (NGS) analyzes entire genomes or transcriptomes to identify genetic mutations and diagnose genetic diseases.
- Protein microarrays analyze protein-protein interactions on a large scale to identify protein biomarkers and understand disease mechanisms.
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Description
This quiz covers DNA manipulation and analysis techniques used in medical biochemistry, including restriction enzymes, DNA ligase, PCR, and more.
