Podcast
Questions and Answers
Which molecular biology technique is utilized to amplify DNA for studying gene expression and mutations?
Which molecular biology technique is utilized to amplify DNA for studying gene expression and mutations?
- Polymerase Chain Reaction (PCR) (correct)
- Western Blotting
- ELISA
- Flow Cytometry
Quantitative PCR (qPCR) measures protein levels in real time.
Quantitative PCR (qPCR) measures protein levels in real time.
False (B)
Which molecular biology technique is used to detect specific proteins in a sample, often utilized to confirm HIV infections?
Which molecular biology technique is used to detect specific proteins in a sample, often utilized to confirm HIV infections?
Western Blotting
__________ is a molecular biology technique that analyzes and sorts cells based on size, shape, and surface markers.
__________ is a molecular biology technique that analyzes and sorts cells based on size, shape, and surface markers.
Match the following techniques with their primary function in cell analysis:
Match the following techniques with their primary function in cell analysis:
What is the primary function of Fluorescence Microscopy in cell analysis?
What is the primary function of Fluorescence Microscopy in cell analysis?
CRISPR-Cas9 is used to amplify genes for studying gene expression.
CRISPR-Cas9 is used to amplify genes for studying gene expression.
What technique is used to examine gene expression patterns in cells to understand the differences between healthy and tumor cells?
What technique is used to examine gene expression patterns in cells to understand the differences between healthy and tumor cells?
___________ are used in personalized medicine to identify genetic mutations that influence drug responses.
___________ are used in personalized medicine to identify genetic mutations that influence drug responses.
Which of the following techniques is used to detect antibodies in HIV and COVID-19 diagnostic tests?
Which of the following techniques is used to detect antibodies in HIV and COVID-19 diagnostic tests?
Mass Spectrometry is primarily used to amplify DNA in forensic science.
Mass Spectrometry is primarily used to amplify DNA in forensic science.
What technique is used to edit genes to study gene function and disease mechanisms?
What technique is used to edit genes to study gene function and disease mechanisms?
The function of __________ is to measure DNA or RNA levels in real time.
The function of __________ is to measure DNA or RNA levels in real time.
In cancer research, comparing gene activity between healthy and tumor cells can be done using which technique?
In cancer research, comparing gene activity between healthy and tumor cells can be done using which technique?
Flow Cytometry is primarily used to visualize the movement of proteins in live cells.
Flow Cytometry is primarily used to visualize the movement of proteins in live cells.
__________ is used to identify and quantify proteins and metabolites in cells.
__________ is used to identify and quantify proteins and metabolites in cells.
Which molecular biology technique is applied in genetic therapy to correct mutations responsible for sickle cell anemia?
Which molecular biology technique is applied in genetic therapy to correct mutations responsible for sickle cell anemia?
What is the primary function of ELISA?
What is the primary function of ELISA?
Which technique is commonly used to study biomarkers for diseases like diabetes?
Which technique is commonly used to study biomarkers for diseases like diabetes?
Polymerase Chain Reaction (PCR) is used only in medical diagnostics to detect viral infections.
Polymerase Chain Reaction (PCR) is used only in medical diagnostics to detect viral infections.
Flashcards
Molecular Biology
Molecular Biology
Molecular biology is the study of biology at a molecular level. It focuses on understanding the interactions between various systems of a cell.
Polymerase Chain Reaction (PCR)
Polymerase Chain Reaction (PCR)
A technique used to amplify DNA, creating many copies from a single template.
Quantitative PCR (qPCR)
Quantitative PCR (qPCR)
A method used to measure the amount of DNA or RNA in real time during PCR amplification.
Western Blotting
Western Blotting
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Flow Cytometry
Flow Cytometry
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Fluorescence Microscopy
Fluorescence Microscopy
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CRISPR-Cas9
CRISPR-Cas9
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RNA Sequencing (RNA-seq)
RNA Sequencing (RNA-seq)
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Microarrays
Microarrays
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ELISA
ELISA
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Mass Spectrometry
Mass Spectrometry
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Study Notes
- Molecular biology techniques are essential for analyzing cells at the genetic, protein, and functional levels.
Techniques used in cell analysis
- Polymerase Chain Reaction (PCR) amplifies DNA to study gene expression and mutations.
- PCR can be used to analyze DNA from crime scenes in forensic science.
- PCR can be used in medical diagnostics to detect viral infections like COVID-19.
- Quantitative PCR (qPCR) measures DNA or RNA levels in real time.
- qPCR is used in cancer research to quantify gene expression changes in tumor cells.
- Western Blotting detects specific proteins in a sample.
- Western blotting is commonly used to confirm HIV infections by detecting viral proteins in blood samples.
- Flow Cytometry analyzes and sorts cells based on size, shape, and surface markers.
- Flow Cytometry can be used in immunology to count and classify different types of immune cells in leukemia patients.
- Fluorescence Microscopy uses fluorescent dyes to visualize cellular structures and processes.
- Fluorescence Microscopy can be used to track the movement of proteins within live cells in neuroscience research.
- CRISPR-Cas9 edits genes to study gene function and disease mechanisms.
- CRISPR-Cas9 can be used in genetic therapy to correct mutations responsible for sickle cell anemia.
- RNA Sequencing (RNA-seq) examines gene expression patterns in cells.
- RNA-seq can be used in cancer research to compare gene activity between healthy and tumor cells.
- Microarrays analyzes gene expression or genetic variations.
- Microarrays can be used in personalized medicine to identify genetic mutations that influence drug responses.
- ELISA (Enzyme-Linked Immunosorbent Assay) detects and quantifies proteins, hormones, or antibodies.
- ELISA can be used in medical diagnostics to detect antibodies in HIV and COVID-19 tests.
- Mass Spectrometry identifies and quantifies proteins and metabolites in cells.
- Mass Spectrometry can be used in metabolomics to study biomarkers for diseases like diabetes.
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