12 Questions
What is the function of the DNA segment known as an exon?
It codes for protein and remains in the mRNA after processing.
What is the term for the DNA sequence that could potentially be translated into a protein?
Open Reading Frame (ORF)
What is the term for the mRNA that carries the information for multiple proteins?
Polycistronic mRNA
What is the co-factor required by DNA-dependent RNA polymerase?
Mg2+
What is the term for the DNA sequence that does not code for proteins or functional RNA molecules?
Noncoding DNA
What is the location of the RNA polymerase?
Cytosol
What is the approximate molecular weight of the RNA polymerase holoenzyme?
460Kda
What is the consensus sequence for the -10 element in prokaryotic promoters?
5' TATAAT 3'
What is the role of the σ factor in the formation of the RNA polymerase holoenzyme?
It binds to the core enzyme and forms the holoenzyme.
What is the approximate length of the transcription bubble formed during the initiation of transcription?
12-14 bp
Which model of transcription initiation is currently believed to reflect what actually happens?
Scrunching
How much DNA does the RNA polymerase holoenzyme cover during the formation of the closed binary complex?
75-80 bp
Test your knowledge on DNA-dependent RNA polymerase, its requirements, and the process of RNA synthesis. Learn about the different stages of RNA synthesis, including the formation of pre-messenger RNA and the production of mature mRNA. Evaluate your understanding of gene expression and its related concepts.
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