Molecular Biology: RNA Synthesis and Gene Expression

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Questions and Answers

What is the function of the DNA segment known as an exon?

  • It is a type of promoter sequence that binds to RNA polymerase.
  • It codes for protein and remains in the mRNA after processing. (correct)
  • It does not code for protein and is removed during processing.
  • It remains in the primary transcript but is removed during processing.

What is the term for the DNA sequence that could potentially be translated into a protein?

  • Operon
  • Promoter sequence
  • Open Reading Frame (ORF) (correct)
  • Intergenic DNA

What is the term for the mRNA that carries the information for multiple proteins?

  • Monocistronic mRNA
  • mRNA
  • Polycistronic mRNA (correct)
  • tRNA

What is the co-factor required by DNA-dependent RNA polymerase?

<p>Mg2+ (B)</p> Signup and view all the answers

What is the term for the DNA sequence that does not code for proteins or functional RNA molecules?

<p>Noncoding DNA (A)</p> Signup and view all the answers

What is the location of the RNA polymerase?

<p>Cytosol (D)</p> Signup and view all the answers

What is the approximate molecular weight of the RNA polymerase holoenzyme?

<p>460Kda (C)</p> Signup and view all the answers

What is the consensus sequence for the -10 element in prokaryotic promoters?

<p>5' TATAAT 3' (A)</p> Signup and view all the answers

What is the role of the σ factor in the formation of the RNA polymerase holoenzyme?

<p>It binds to the core enzyme and forms the holoenzyme. (C)</p> Signup and view all the answers

What is the approximate length of the transcription bubble formed during the initiation of transcription?

<p>12-14 bp (C)</p> Signup and view all the answers

Which model of transcription initiation is currently believed to reflect what actually happens?

<p>Scrunching (C)</p> Signup and view all the answers

How much DNA does the RNA polymerase holoenzyme cover during the formation of the closed binary complex?

<p>75-80 bp (D)</p> Signup and view all the answers

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