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Molecular Biology: RNA Synthesis and Gene Expression

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Molecular Biology: RNA Synthesis and Gene Expression

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Questions and Answers

What is the function of the DNA segment known as an exon?

It is a type of promoter sequence that binds to RNA polymerase.

It codes for protein and remains in the mRNA after processing. (correct)

It does not code for protein and is removed during processing.

It remains in the primary transcript but is removed during processing.

What is the term for the DNA sequence that could potentially be translated into a protein?

Operon

Promoter sequence

Open Reading Frame (ORF) (correct)

Intergenic DNA

What is the term for the mRNA that carries the information for multiple proteins?

Monocistronic mRNA

mRNA

Polycistronic mRNA (correct)

tRNA

What is the co-factor required by DNA-dependent RNA polymerase?

Mg2+ (B) Signup and view all the answers

What is the term for the DNA sequence that does not code for proteins or functional RNA molecules?

Noncoding DNA (A) Signup and view all the answers

What is the location of the RNA polymerase?

Cytosol (D) Signup and view all the answers

What is the approximate molecular weight of the RNA polymerase holoenzyme?

460Kda (C) Signup and view all the answers

What is the consensus sequence for the -10 element in prokaryotic promoters?

5' TATAAT 3' (A) Signup and view all the answers

What is the role of the σ factor in the formation of the RNA polymerase holoenzyme?

It binds to the core enzyme and forms the holoenzyme. (C) Signup and view all the answers

What is the approximate length of the transcription bubble formed during the initiation of transcription?

12-14 bp (C) Signup and view all the answers

Which model of transcription initiation is currently believed to reflect what actually happens?

Scrunching (C) Signup and view all the answers

How much DNA does the RNA polymerase holoenzyme cover during the formation of the closed binary complex?

75-80 bp (D) Signup and view all the answers