Molecular Biology: RNA Synthesis and Gene Expression
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Questions and Answers

What is the function of the DNA segment known as an exon?

  • It is a type of promoter sequence that binds to RNA polymerase.
  • It codes for protein and remains in the mRNA after processing. (correct)
  • It does not code for protein and is removed during processing.
  • It remains in the primary transcript but is removed during processing.
  • What is the term for the DNA sequence that could potentially be translated into a protein?

  • Operon
  • Promoter sequence
  • Open Reading Frame (ORF) (correct)
  • Intergenic DNA
  • What is the term for the mRNA that carries the information for multiple proteins?

  • Monocistronic mRNA
  • mRNA
  • Polycistronic mRNA (correct)
  • tRNA
  • What is the co-factor required by DNA-dependent RNA polymerase?

    <p>Mg2+</p> Signup and view all the answers

    What is the term for the DNA sequence that does not code for proteins or functional RNA molecules?

    <p>Noncoding DNA</p> Signup and view all the answers

    What is the location of the RNA polymerase?

    <p>Cytosol</p> Signup and view all the answers

    What is the approximate molecular weight of the RNA polymerase holoenzyme?

    <p>460Kda</p> Signup and view all the answers

    What is the consensus sequence for the -10 element in prokaryotic promoters?

    <p>5' TATAAT 3'</p> Signup and view all the answers

    What is the role of the σ factor in the formation of the RNA polymerase holoenzyme?

    <p>It binds to the core enzyme and forms the holoenzyme.</p> Signup and view all the answers

    What is the approximate length of the transcription bubble formed during the initiation of transcription?

    <p>12-14 bp</p> Signup and view all the answers

    Which model of transcription initiation is currently believed to reflect what actually happens?

    <p>Scrunching</p> Signup and view all the answers

    How much DNA does the RNA polymerase holoenzyme cover during the formation of the closed binary complex?

    <p>75-80 bp</p> Signup and view all the answers

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