Molecular Biology Quiz
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Questions and Answers

What is the primary function of ribozymes in RNA?

  • To store genetic information
  • To transport amino acids
  • To catalyze chemical reactions (correct)
  • To replicate DNA

Which of the following statements accurately describes the structure of DNA?

  • DNA contains ribose sugar in its backbone.
  • DNA base pairs A-U and C-G.
  • DNA is made up of a single strand of nucleotides.
  • DNA consists of two anti-parallel strands coiled around histones. (correct)

In Meselson and Stahl's experiment, what was the purpose of using heavy nitrogen (15N)?

  • To create a density gradient for separating DNA molecules (correct)
  • To facilitate faster DNA replication
  • To reduce the activity of bacteria
  • To label newly synthesized nucleotides only

What is the key feature of hydrogen bonds in the DNA structure?

<p>They are weak individually but collectively strong (D)</p> Signup and view all the answers

Which type of RNA carries genetic information from DNA to the ribosome for protein synthesis?

<p>mRNA (C)</p> Signup and view all the answers

What is the primary role of helicase during DNA replication?

<p>To unwind the DNA double helix (B)</p> Signup and view all the answers

Which of the following statements about leading and lagging strands is correct?

<p>Lagging strand is made in fragments called Okazaki fragments. (D)</p> Signup and view all the answers

What is the function of ligase in DNA replication?

<p>To join Okazaki fragments together (A)</p> Signup and view all the answers

How does DNA polymerase III initiate replication?

<p>It requires existing nucleotides to start extending. (A)</p> Signup and view all the answers

What typically marks the starting point of DNA replication?

<p>AT-rich regions due to weaker bonds (C)</p> Signup and view all the answers

Which event occurs during transcription in protein synthesis?

<p>mRNA copies the code of a gene (D)</p> Signup and view all the answers

What is the outcome of a slippage error during DNA replication?

<p>An additional gene is created (D)</p> Signup and view all the answers

Which protein is responsible for cutting out RNA primers during DNA replication?

<p>DNA Polymerase I (D)</p> Signup and view all the answers

What distinguishes the process of transcription from translation?

<p>Transcription produces mRNA from DNA. (B)</p> Signup and view all the answers

In the central dogma of molecular biology, what is the correct order of information flow?

<p>DNA → mRNA → Protein (D)</p> Signup and view all the answers

Flashcards

Nucleotide

The monomer unit of DNA and RNA, composed of a base, a phosphate group, and a sugar.

Complementary Bases

Specific pairs of bases (A-T, C-G) that bond together in DNA and RNA, holding the double helix together.

Semi-conservative Replication

DNA replication method where each daughter DNA molecule consists of one original strand and one new strand.

DNA Replication - Meselson and Stahl Experiment

A crucial experiment proving the semi-conservative model of DNA replication. It used isotopes to track DNA strands across cell generations.

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Nucleosome

A structural unit of DNA packaging in eukaryotes, where DNA coils around histone proteins.

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DNA Replication

The process of creating two identical DNA molecules from one original DNA molecule.

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Helicase

Enzyme that unwinds the DNA double helix during replication, creating replication forks.

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Replication Forks

Y-shaped regions where the DNA double helix separates during replication.

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Single-strand binding proteins

Proteins that bind to the separated DNA strands to prevent them from re-annealing.

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DNA Polymerase III

Enzyme that synthesizes new DNA strands by adding nucleotides to the 3' end of a pre-existing primer.

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Okazaki Fragments

Short DNA fragments synthesized on the lagging strand during DNA replication.

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DNA Polymerase I

Enzyme that removes RNA primers and replaces them with DNA during replication.

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Ligase

Enzyme that joins Okazaki fragments together on the lagging strand.

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Leading Strand

New DNA strand synthesized continuously in the 5' to 3' direction.

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Lagging Strand

New DNA strand synthesized discontinuously in short fragments (Okazaki fragments).

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