Molecular Biology Quiz
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Questions and Answers

What is the correct flow of genetic information in cells?

  • DNA to RNA to protein (correct)
  • RNA to DNA to protein
  • DNA to protein to RNA
  • Protein to DNA to RNA
  • Which of the following would be synthesized using 5'-CAGTTCGGA-3' as a template?

  • 3'-GTCAAGCCT-5'
  • 3'-AGGCTTGAC-4'
  • 3'-CAGTTCGGA-5'
  • 3'-TCCGAACTG-5' (correct)
  • During elongation in translation, to which ribosomal site does an incoming charged tRNA molecule bind?

  • A site (correct)
  • E site
  • P site
  • B site
  • During which stage of bacterial transcription is the sigma subunit of the RNA polymerase involved?

    <p>Initiation</p> Signup and view all the answers

    Which site initiates the immune response?

    <p>Lymph node</p> Signup and view all the answers

    Which cells are the source of Interferon-γ (IFN-γ)?

    <p>Natural killer cells</p> Signup and view all the answers

    Which compound is produced by activated macrophages through an oxygen-dependent mechanism?

    <p>Nitric oxide</p> Signup and view all the answers

    Which immunoglobulin is the first produced after antigen exposure (e.g., bacteria)?

    <p>Ig M</p> Signup and view all the answers

    What is the correct flow of genetic information in cells?

    <p>DNA to RNA to protein</p> Signup and view all the answers

    Which enzymes do not need water for reactions?

    <p>Layases</p> Signup and view all the answers

    Which sequence would be synthesized using 5'-CAGTTCGGA-3' as a template?

    <p>3'-TCCGAACTG-5'</p> Signup and view all the answers

    During elongation in translation, to which ribosomal site does an incoming charged tRNA molecule bind?

    <p>A site</p> Signup and view all the answers

    What is the important nutrient for bacterial teichoic acid?

    <p>PO4</p> Signup and view all the answers

    Which bacterial group could not grow below 55°C?

    <p>Hyperthermophile</p> Signup and view all the answers

    During which stage of bacterial transcription is the σ subunit of the RNA polymerase involved?

    <p>Initiation</p> Signup and view all the answers

    Which type of transcriptional control can regulate both inducible and repressible genes?

    <p>Both negative and positive transcriptional control</p> Signup and view all the answers

    Which metabolic pathway is used by bacteria but not by eukaryotes?

    <p>Entner-Doudoroff</p> Signup and view all the answers

    Which of the following are storage inclusions?

    <p>Polyhydroxyalkonate</p> Signup and view all the answers

    Which cytokine is primarily produced by natural killer cells?

    <p>IFN-γ</p> Signup and view all the answers

    All of the following statements regarding penicillins are true EXCEPT:

    <p>Penicillins don't require dosage adjustment in renal failure.</p> Signup and view all the answers

    Which of the following is considered to be bacteriostatic?

    <p>Chloramphenicol</p> Signup and view all the answers

    Which among the following antibiotics functions as a protein synthesis inhibitor?

    <p>Tetracycline</p> Signup and view all the answers

    Benzylpenicillin is the chemical name for which of the following penicillin?

    <p>Penicillin G</p> Signup and view all the answers

    What is a common feature among penicillin, cephalosporins, carbapenems, and monobactams?

    <p>They all have a β-lactam ring as a part of their biological interaction</p> Signup and view all the answers

    Which of the following fermentation processes is used in the production of penicillin?

    <p>Aerobic fermentation</p> Signup and view all the answers

    Which of the following combinations would most likely contribute to the development of a superinfection?

    <p>Long-term use of broad-spectrum antimicrobials</p> Signup and view all the answers

    The F plasmid is involved in which of the following processes?

    <p>Conjugation</p> Signup and view all the answers

    The formation of pyrimidine dimers results from which of the following?

    <p>Exposure to ultraviolet radiation</p> Signup and view all the answers

    Which of the following is not involved in the initiation of replication?

    <p>Ligase</p> Signup and view all the answers

    Which of the following conditions leads to maximal expression of the lac operon?

    <p>Lactose present, glucose absent</p> Signup and view all the answers

    Which type of agar is used for growing Salmonella?

    <p>Desoxycholate agar</p> Signup and view all the answers

    What does E.coli secrete?

    <p>Heat stable toxin</p> Signup and view all the answers

    What types of antigens are present in Enterobacteriaceae?

    <p>All of above</p> Signup and view all the answers

    Klebsiella has which antigens?

    <p>K antigen</p> Signup and view all the answers

    Which sugar conversion is associated with Lactobacillus?

    <p>Lactic</p> Signup and view all the answers

    What types of diseases can Serratia cause?

    <p>All of them</p> Signup and view all the answers

    Which types of anthrax exist?

    <p>All of the above</p> Signup and view all the answers

    What type of media is EMB?

    <p>Differential media</p> Signup and view all the answers

    Study Notes

    Flow of Genetic Information in Cells

    • DNA to RNA to protein is the correct sequence of genetic information flow
    • The other options are incorrect

    Synthesized Sequence

    • The correct synthesized sequence is 3'-AGGCTTGAC-4'

    Ribosomal Site

    • During elongation in translation, an incoming charged tRNA molecule binds to the A site of the ribosome

    Bacterial Transcription Stage

    • The sigma subunit of the RNA polymerase is involved in the initiation stage of bacterial transcription

    Transcriptional Control

    • Negative transcriptional control represses gene expression, whereas positive transcriptional control activates gene expression
    • Both types of control can regulate inducible and repressible genes

    Immunity

    • Innate immunity is the first defense and is antigen-independent
    • Thymus is the site for maturation of T cells, but not B cells
    • Peyer patch is a mini lymph node
    • Macrophages are not the only source of interleukin-6 (IL-6)
    • Ig M antibodies play a role in protection against helminth infection

    Immune Response

    • The site where immune response is initiated is the thymus
    • Natural killer cells are the source of Interferon-y (IFN-y)
    • Macrophages activated to produce oxygen-dependent compounds produce nitric oxide
    • The first immunoglobulin produced after antigen exposure is Ig A
    • Antigen is the substance capable of eliciting a specific immune response

    Antibiotics

    • Benzylpenicillin is the chemical name for Penicillin G
    • A common feature among the antibiotics like penicillin, cephalosporins, carbapenems, and monobactams is that they all have a β-lactam ring as a part of their biological interaction
    • Aerobic fermentation is used in the production of penicillin
    • Long-term use of broad-spectrum antimicrobials can contribute to the development of a superinfection

    Fermentation

    • Spherooplast and protoplast cells can live in a medium containing a high concentration of sucrose solution
    • RNA POLYMERASE enzyme is an ideal target for antibiotics such as REFA, which leads to the killing of bacteria

    Genetics

    • The F plasmid is involved in the process of conjugation
    • The formation of pyrimidine dimers results from exposure to ultraviolet radiation
    • Ligase is not involved in the initiation of replication
    • The conditions for maximal expression of the lac operon are lactose present and glucose absent
    • A deletion of a codon is an example of a frameshift mutation
    • The promoter is involved in the initiation of transcription

    Biology Exam

    • Transferases are the enzymes that do not need water for reactions
    • Mg is the important nutrient for bacterial techoic acid
    • Thermophel is the bacterial group that cannot grow at less than 55°C
    • The Enter-Doudoroff pathway is used by bacteria but not by eukaryotes
    • Carboxysomes are an example of storage inclusions

    Antibiotics

    • Most penicillins only cross the blood-brain barrier when the meninges are inflamed
    • Penicillins inhibit the cross-linkage of peptidoglycans in the cell wall
    • Piperacillin is a penicillin active against pseudomonas
    • Only about 5 to 10% of people with a past history of penicillin allergy have a reaction on re-exposure
    • Norfloxacillin, Chloramphenicol, Trimethoprim, and Rifampicin are all antibiotics that inhibit nucleic acid synthesis
    • Sulfasalazin is not an antibiotic that inhibits nucleic acid synthesis
    • Concerning toxicity of antibiotics, enamel dysplasia is common with aminoglycosides, Grey Baby Syndrome occurs with rifampicin use, a disulfiram-like reaction can occur with macrolides, and haemolytic anaemias can occur with sulphonamide use
    • Chloramphenicol is a bacteriostatic antibiotic
    • Metronidazole is used to treat giardia, causes a metallic taste in the mouth, inhibits alcohol dehydrogenase, and is used to treat gardnerella and trichomonas vaginalis
    • Tetracycline is an antibiotic that functions as a protein synthesis inhibitor

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    Test your understanding of genetic information flow, transcription, and translation in cells. Questions cover molecular biology concepts, including DNA, RNA, and protein synthesis.

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