Molecular Biology Quiz
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Questions and Answers

What is the primary function of histones in DNA packaging?

  • They catalyze the formation of phosphodiester bonds in DNA replication.
  • They act as enzymes that unwind DNA.
  • They are involved in the synthesis of mRNA.
  • They bind to DNA and help condense it into chromatin. (correct)
  • Which of the following statements accurately describes the process of DNA replication?

  • It is a random process, with no specific pattern for the distribution of old and new DNA strands.
  • It is a semi-conservative process, where each new DNA molecule contains one original strand and one newly synthesized strand. (correct)
  • It is a conservative process, producing two identical DNA molecules, one completely new and one completely old.
  • It is a dispersive process, with each new DNA molecule containing a mix of old and new DNA segments.
  • Why is a primer required for DNA polymerase to initiate DNA synthesis?

  • The primer provides the necessary energy for the reaction.
  • The primer helps to stabilize the DNA molecule during replication.
  • DNA polymerase can only add nucleotides to an existing 3'-OH group. (correct)
  • The primer provides a template strand for DNA polymerase to use.
  • What is the significance of the wobble position in tRNA?

    <p>It allows for a single tRNA to recognize multiple codons. (C)</p> Signup and view all the answers

    Which of the following statements is TRUE about introns?

    <p>Introns are transcribed but not translated. (A)</p> Signup and view all the answers

    What is the main difference between the structure of DNA and RNA?

    <p>DNA contains ribose sugar, while RNA contains deoxyribose sugar. (B), DNA contains thymine (T), while RNA contains uracil (U). (C)</p> Signup and view all the answers

    What is the significance of the experiment conducted by Avery, MacLeod, and McCarty?

    <p>They demonstrated that DNA is the genetic material. (A)</p> Signup and view all the answers

    If a sample of DNA contains 20% adenine (A), what percentage of guanine (G) would you expect to find?

    <p>40% (D)</p> Signup and view all the answers

    Which enzyme is responsible for unwinding the DNA double helix during replication?

    <p>DNA helicase (A)</p> Signup and view all the answers

    What is the primary function of primase in DNA replication?

    <p>To provide a starting point for DNA polymerase (D)</p> Signup and view all the answers

    What is the main difference between the leading and lagging strands during DNA replication?

    <p>The leading strand is synthesized continuously, while the lagging strand is synthesized in fragments. (B)</p> Signup and view all the answers

    What role does DNA ligase play in DNA replication?

    <p>It connects the Okazaki fragments together (B)</p> Signup and view all the answers

    Which of the following is NOT a characteristic of RNA polymerase?

    <p>It can synthesize DNA during replication (D)</p> Signup and view all the answers

    Which of the following experiments provided evidence for the structure of DNA as a double helix?

    <p>Rosalind Franklin's X-ray diffraction data revealing the helical nature of DNA (A)</p> Signup and view all the answers

    What is the complementary sequence of 5'-AATTCGCTTA-3'?

    <p>5'-TAAGCGAATT-3' (A)</p> Signup and view all the answers

    What is the function of the sigma factor in bacterial transcription?

    <p>It binds to the promoter region and helps RNA polymerase initiate transcription (D)</p> Signup and view all the answers

    Which of the following is NOT a consensus sequence involved in prokaryotic transcription initiation?

    <p>Shine-Dalgarno (C)</p> Signup and view all the answers

    What is the primary function of the sigma factor in prokaryotic transcription initiation?

    <p>It recognizes and binds to the promoter region. (B)</p> Signup and view all the answers

    Which of the following is the correct order of events during DNA replication?

    <p>DNA helicase, primase, DNA polymerase, DNA ligase (D)</p> Signup and view all the answers

    Which of the following statements accurately describes the role of telomerase in maintaining chromosome length?

    <p>Telomerase prevents the shortening of chromosomes by adding telomeric repeats to the 3' end. (A)</p> Signup and view all the answers

    Which of the following is NOT a characteristic of telomerase?

    <p>It is involved in DNA repair. (C)</p> Signup and view all the answers

    In the replication fork diagram, which component represents the leading strand?

    <p>E (B)</p> Signup and view all the answers

    Where does splicing of transcripts normally occur?

    <p>Only in the nucleus of eukaryotes. (D)</p> Signup and view all the answers

    What is the likely consequence of a mutation in the telomerase enzyme that renders it nonfunctional?

    <p>Chromosome length will gradually decrease. (C)</p> Signup and view all the answers

    What is the primary function of the spliceosome?

    <p>It removes introns from primary mRNA. (A)</p> Signup and view all the answers

    What did Watson and Crick suggest about the structure of DNA in their 1953 Nature paper?

    <p>A copying mechanism for the genetic material. (D)</p> Signup and view all the answers

    How many amino acids are specified by the following mRNA sequence: 5’-ACCGACAUCGUCGUAGCAACUUUUUGGGGACCC-3’?

    <p>10 (B)</p> Signup and view all the answers

    Given the template strand of a gene is 3’-TAGCAGAATCGGCA-5’, what is the corresponding mRNA sequence?

    <p>5’-ACGGCUAAGACGAU-3’ (A)</p> Signup and view all the answers

    What is the primary reason for the discrepancy between the number of protein-coding genes in the human genome (approximately 20,000) and the number of gene products (several hundred thousand)?

    <p>The ability of a single gene to produce multiple proteins through alternative splicing. (A)</p> Signup and view all the answers

    Which of the following is NOT a function of the 5' cap on eukaryotic mRNA?

    <p>It facilitates 5' intron excision. (A)</p> Signup and view all the answers

    What is the name of the sequence that ribosomes bind to in prokaryotes?

    <p>Shine-Dalgarno sequence (B)</p> Signup and view all the answers

    Which of these is NOT a characteristic of the spliceosome?

    <p>It binds to the 5' cap of mRNA. (C)</p> Signup and view all the answers

    What is the role of single-stranded binding proteins in DNA replication?

    <p>They prevent the unwound DNA strands from re-annealing. (C)</p> Signup and view all the answers

    Which of the following describes the role of heterochromatin in eukaryotic chromosomes?

    <p>Heterochromatin is highly condensed and transcriptionally inactive. (D)</p> Signup and view all the answers

    What is the function of the promoter in gene expression?

    <p>The promoter is the binding site for RNA polymerase, initiating transcription. (B)</p> Signup and view all the answers

    What is the difference in function between DNA helicases and topoisomerases in DNA replication?

    <p>Helicases unwind the DNA double helix, while topoisomerases prevent supercoiling. (C)</p> Signup and view all the answers

    Which of the following is true regarding tRNA?

    <p>tRNA contains an anticodon loop that pairs with mRNA codons during translation. (B)</p> Signup and view all the answers

    The genetic code is considered to be degenerate. What does this mean?

    <p>Multiple codons can code for the same amino acid. (C)</p> Signup and view all the answers

    Which of the following is NOT a component involved in the compaction of eukaryotic chromosomes to form a metaphase chromosome?

    <p>Coiling of the DNA double helix. (A)</p> Signup and view all the answers

    Which of the following statements best describes the Shine-Dalgarno sequence?

    <p>It is a sequence found in prokaryotic mRNA that helps align the ribosome for translation initiation. (C)</p> Signup and view all the answers

    Flashcards

    Histones

    Proteins that help package DNA and are positively charged, interacting with negatively charged DNA.

    tRNA Anticodon Matching

    At least 3 bases of tRNA's anticodon must pair with mRNA's codon for translation.

    DNA Polymerases Requirements

    DNA polymerases need a primer, template DNA, and dNTPs to synthesize DNA.

    E. coli Genetic Material

    E. coli has circular, double-stranded DNA as its genetic material.

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    DNase Treatment in Transformation

    DNase treatment showed that DNA is the transforming principle by preventing transformation.

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    Adenine Content Calculation

    If adenine is 25%, then G+C content in fungus DNA is also 50%, according to Chargaff's rules.

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    Introns

    Introns are non-coding regions removed from mRNA during splicing by snRNPs.

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    Griffith’s Experiment Observation

    Griffith found that mice died when injected with a mix of dead virulent and live non-virulent bacteria.

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    Complementary DNA sequence

    The complementary sequence of 5'-AATTCGCTTA-3' is 5'-TAAGCGAATT-3'.

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    Prokaryotic transcription initiation

    Consensus sequences -10 and -35 are important for RNA polymerase binding with sigma factor.

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    Telomerase function

    Telomerase synthesizes telomeric repeats at chromosome ends and is made of RNA and protein.

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    Replication fork components

    Leading strand, Okazaki fragment, RNA primer, lagging strand, template strand.

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    Splicing location

    Splicing of transcripts occurs only in the nucleus of eukaryotes.

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    Effect of telomerase mutation

    A nonfunctional telomerase leads to gradual decrease in chromosome length over mitosis.

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    General transcription factors

    They recognize the TATA box and recruit RNA polymerase II in eukaryotes.

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    Function of RNA polymerase sigma factor

    The sigma factor aids in the initiation of transcription in prokaryotes by binding to promoter regions.

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    Shine-Dalgarno sequence

    A ribosomal binding site in bacterial mRNA that initiates translation.

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    Spliceosome

    A complex that removes introns from pre-mRNA and performs splicing.

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    Watson and Crick's DNA structure

    Proposed the double helix mechanism explaining DNA replication.

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    Amino acids in mRNA translation

    The number of amino acids coded in a specific mRNA transcript.

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    mRNA transcript from DNA

    The sequence of RNA synthesized from a given DNA coding strand.

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    Alternative splicing

    A process that allows a single gene to produce multiple proteins by varying which exons are included.

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    5' cap function

    Protects eukaryotic mRNA and aids translation initiation.

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    Gene vs. gene products ratio

    The phenomenon where one gene can lead to many proteins due to processes like splicing.

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    Virulent bacteria transformation

    Non-virulent bacteria can become virulent when they take up DNA from virulent bacteria.

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    Compaction of metaphase chromosomes

    Compaction involves nucleosome formation and further compaction of radial loops.

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    Promoter region of a gene

    It contains cis-acting sequences and consensus sequences for RNA polymerase binding.

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    Maintaining open DNA template

    Single-stranded binding proteins keep the DNA template open during replication.

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    Eukaryotic chromosome domains

    Chromosomes have heterochromatin (tightly packed) and euchromatin (loosely packed) regions.

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    Characteristics of tRNA

    tRNA is encoded by tRNA genes, has an anticodon loop, and accepts amino acids.

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    Genetic code codons

    There can be multiple codons for a single amino acid, but not vice versa.

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    Lagging vs. Leading strand

    The leading strand synthesizes continuously 5' to 3'; the lagging strand synthesizes discontinuously.

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    Leading Strand

    The DNA strand that is synthesized continuously during replication.

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    Lagging Strand

    The DNA strand that is synthesized in short segments, requiring multiple primers.

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    Okazaki Fragments

    Short DNA segments formed on the lagging strand during replication.

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    DNA Helicase

    An enzyme that unwinds the DNA double helix during replication.

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    Sigma Factor

    A protein that helps RNA polymerase bind to the promoter to initiate transcription.

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    RNA Polymerase Features

    Enzymes that transcribe DNA into RNA and do not require a primer.

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    Chargaff's Rules

    Observations that DNA has equal amounts of purines and pyrimidines.

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    Helicase Speed

    The observation that helicase operates faster on the leading strand than on the lagging strand.

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    Study Notes

    Histones and DNA Charge

    • Histones have a positive charge, which helps their interaction with DNA, which has a negative charge.

    tRNA Structure and Codon Pairing

    • tRNA molecules have stems and loops.
    • The anticodon loop of tRNA pairs with a codon on mRNA.
    • At least one, or possibly two, bases on the anticodon loop must pair exactly with the bases of the codon, due to the wobble position.

    DNA Polymerase Requirements

    • DNA polymerases need a primer, template DNA, and deoxynucleoside triphosphates (dNTPs) to synthesize DNA.

    E. coli Genetic Material

    • The genetic material in E. coli is composed of circular, double-stranded DNA.

    Avery, MacLeod, and McCarty Experiment

    • The DNase treatment, used by Avery, MacLeod, and McCarty, allowed them to isolate pure DNA samples.
    • It demonstrated that removing DNA prevents transformation.

    Adenine Content and G+C Percentage

    • If DNA has a 25% adenine content, the G+C content will be 50%.

    Introns

    • Introns are removed from primary mRNA before translation.
    • Introns form a lariat structure during splicing.
    • Introns have specific nucleotide sequences at their 5' and 3' ends.

    Griffith's 1928 Experiment

    • Griffith's experiment demonstrated genetic transformation of non-virulent bacteria into virulent bacteria.
    • Mice injected with a mixture of dead virulent bacteria and live non-virulent bacteria died.

    Eukaryotic Chromosome Compaction

    • Eukaryotic chromosomes involve the formation of nucleosomes, a 30nm fiber, and further compaction of radial loops.

    Promoter Region

    • The promoter region in a gene contains cis-acting sequences and consensus sequences for RNA polymerase binding.

    DNA Replication

    • Single-stranded binding proteins maintain the DNA template "open" during replication.
    • DNA polymerases are also involved.

    RNA

    • tRNA is an RNA molecule that is encoded by tRNA genes.
    • It is translated into protein in the cytoplasm.
    • tRNA has secondary structure and an amino acid accepting site.

    Genetic Code

    • The genetic code can have multiple (more than one) codons that code for a single amino acid.
    • Multiple start codons may exist.
    • The genetic code is ambiguous when the same codon codes for two or more amino acids.

    RNA Polymerase

    • RNA polymerase needs a promoter sequence for binding to initiate transcription and synthesize mRNA.
    • Sigma factor helps RNA polymerase find and bind to promoter elements.

    RNA Polymerase and DNA

    • RNA polymerase is responsible for transcribing DNA into RNA.
    • It does not require a primer to synthesize in the 5' to 3' direction.
    • RNA polymerase uses ribonucleotides as substrate.

    DNA Polymerases

    • DNA polymerases require a primer and template for replication to start.

    Mutations and DNA Synthesis

    • DNA mutations in polymerases may affect the directional synthesis of DNA (the 3' to 5' direction).

    Telomerase

    • Telomerase synthesizes telomeric repeats at the ends of chromosomes.
    • It uses RNA as a template for DNA synthesis.
    • It binds to the 3’ overhang of the telomere.

    Replication Fork

    • DNA replication occurs at a replication fork.

    Splicing in Prokaryotes and Eukaryotes

    • Splicing of transcripts occurs in the nucleus of eukaryotes but not in the cytoplasm of prokaryotes.

    Telomere Mutations

    • Mutations in telomerase result in a gradual decrease of chromosome length during cell division.

    Transcription Factors

    • General transcription factors in eukaryotes recognize the TATA box and recruit RNA polymerase II.

    Spliceosome and Introns

    • The spliceosome removes introns from primary mRNA.
    • The spliceosome recognizes nucleotides within the introns, forming a lariat structure during splicing.

    DNA Replication and RNA Enzymes

    • RNA polymerases copy genetic information during DNA replication, using RNA-based primers where applicable.

    Eukaryotic mRNA Structure

    • Eukaryotic mRNA is capped at the 5' end to protect it and aid in transport from the nucleus.

    mRNA Translation

    • A critical part of mRNA involved in translation is the 5' cap.

    Eukaryotic Gene Regulation

    • Eukaryotic genes have a large number of introns, that interrupt the coding sequences of exons.
    • This contributes to the vast number and diversity in gene products from a relatively lower number of genes.

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    Description

    Test your knowledge on key concepts of molecular biology, including histones, tRNA structure, DNA polymerase, and genetic material in E. coli. This quiz covers essential experiments and nucleotide composition, along with the implications of introns. Dive into the intricate world of genetics and molecular interactions!

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