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Questions and Answers
What is the primary function of histones in DNA packaging?
What is the primary function of histones in DNA packaging?
Which of the following statements accurately describes the process of DNA replication?
Which of the following statements accurately describes the process of DNA replication?
Why is a primer required for DNA polymerase to initiate DNA synthesis?
Why is a primer required for DNA polymerase to initiate DNA synthesis?
What is the significance of the wobble position in tRNA?
What is the significance of the wobble position in tRNA?
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Which of the following statements is TRUE about introns?
Which of the following statements is TRUE about introns?
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What is the main difference between the structure of DNA and RNA?
What is the main difference between the structure of DNA and RNA?
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What is the significance of the experiment conducted by Avery, MacLeod, and McCarty?
What is the significance of the experiment conducted by Avery, MacLeod, and McCarty?
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If a sample of DNA contains 20% adenine (A), what percentage of guanine (G) would you expect to find?
If a sample of DNA contains 20% adenine (A), what percentage of guanine (G) would you expect to find?
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Which enzyme is responsible for unwinding the DNA double helix during replication?
Which enzyme is responsible for unwinding the DNA double helix during replication?
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What is the primary function of primase in DNA replication?
What is the primary function of primase in DNA replication?
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What is the main difference between the leading and lagging strands during DNA replication?
What is the main difference between the leading and lagging strands during DNA replication?
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What role does DNA ligase play in DNA replication?
What role does DNA ligase play in DNA replication?
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Which of the following is NOT a characteristic of RNA polymerase?
Which of the following is NOT a characteristic of RNA polymerase?
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Which of the following experiments provided evidence for the structure of DNA as a double helix?
Which of the following experiments provided evidence for the structure of DNA as a double helix?
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What is the complementary sequence of 5'-AATTCGCTTA-3'?
What is the complementary sequence of 5'-AATTCGCTTA-3'?
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What is the function of the sigma factor in bacterial transcription?
What is the function of the sigma factor in bacterial transcription?
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Which of the following is NOT a consensus sequence involved in prokaryotic transcription initiation?
Which of the following is NOT a consensus sequence involved in prokaryotic transcription initiation?
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What is the primary function of the sigma factor in prokaryotic transcription initiation?
What is the primary function of the sigma factor in prokaryotic transcription initiation?
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Which of the following is the correct order of events during DNA replication?
Which of the following is the correct order of events during DNA replication?
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Which of the following statements accurately describes the role of telomerase in maintaining chromosome length?
Which of the following statements accurately describes the role of telomerase in maintaining chromosome length?
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Which of the following is NOT a characteristic of telomerase?
Which of the following is NOT a characteristic of telomerase?
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In the replication fork diagram, which component represents the leading strand?
In the replication fork diagram, which component represents the leading strand?
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Where does splicing of transcripts normally occur?
Where does splicing of transcripts normally occur?
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What is the likely consequence of a mutation in the telomerase enzyme that renders it nonfunctional?
What is the likely consequence of a mutation in the telomerase enzyme that renders it nonfunctional?
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What is the primary function of the spliceosome?
What is the primary function of the spliceosome?
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What did Watson and Crick suggest about the structure of DNA in their 1953 Nature paper?
What did Watson and Crick suggest about the structure of DNA in their 1953 Nature paper?
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How many amino acids are specified by the following mRNA sequence: 5’-ACCGACAUCGUCGUAGCAACUUUUUGGGGACCC-3’?
How many amino acids are specified by the following mRNA sequence: 5’-ACCGACAUCGUCGUAGCAACUUUUUGGGGACCC-3’?
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Given the template strand of a gene is 3’-TAGCAGAATCGGCA-5’, what is the corresponding mRNA sequence?
Given the template strand of a gene is 3’-TAGCAGAATCGGCA-5’, what is the corresponding mRNA sequence?
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What is the primary reason for the discrepancy between the number of protein-coding genes in the human genome (approximately 20,000) and the number of gene products (several hundred thousand)?
What is the primary reason for the discrepancy between the number of protein-coding genes in the human genome (approximately 20,000) and the number of gene products (several hundred thousand)?
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Which of the following is NOT a function of the 5' cap on eukaryotic mRNA?
Which of the following is NOT a function of the 5' cap on eukaryotic mRNA?
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What is the name of the sequence that ribosomes bind to in prokaryotes?
What is the name of the sequence that ribosomes bind to in prokaryotes?
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Which of these is NOT a characteristic of the spliceosome?
Which of these is NOT a characteristic of the spliceosome?
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What is the role of single-stranded binding proteins in DNA replication?
What is the role of single-stranded binding proteins in DNA replication?
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Which of the following describes the role of heterochromatin in eukaryotic chromosomes?
Which of the following describes the role of heterochromatin in eukaryotic chromosomes?
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What is the function of the promoter in gene expression?
What is the function of the promoter in gene expression?
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What is the difference in function between DNA helicases and topoisomerases in DNA replication?
What is the difference in function between DNA helicases and topoisomerases in DNA replication?
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Which of the following is true regarding tRNA?
Which of the following is true regarding tRNA?
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The genetic code is considered to be degenerate. What does this mean?
The genetic code is considered to be degenerate. What does this mean?
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Which of the following is NOT a component involved in the compaction of eukaryotic chromosomes to form a metaphase chromosome?
Which of the following is NOT a component involved in the compaction of eukaryotic chromosomes to form a metaphase chromosome?
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Which of the following statements best describes the Shine-Dalgarno sequence?
Which of the following statements best describes the Shine-Dalgarno sequence?
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Flashcards
Histones
Histones
Proteins that help package DNA and are positively charged, interacting with negatively charged DNA.
tRNA Anticodon Matching
tRNA Anticodon Matching
At least 3 bases of tRNA's anticodon must pair with mRNA's codon for translation.
DNA Polymerases Requirements
DNA Polymerases Requirements
DNA polymerases need a primer, template DNA, and dNTPs to synthesize DNA.
E. coli Genetic Material
E. coli Genetic Material
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DNase Treatment in Transformation
DNase Treatment in Transformation
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Adenine Content Calculation
Adenine Content Calculation
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Introns
Introns
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Griffith’s Experiment Observation
Griffith’s Experiment Observation
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Complementary DNA sequence
Complementary DNA sequence
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Prokaryotic transcription initiation
Prokaryotic transcription initiation
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Telomerase function
Telomerase function
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Replication fork components
Replication fork components
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Splicing location
Splicing location
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Effect of telomerase mutation
Effect of telomerase mutation
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General transcription factors
General transcription factors
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Function of RNA polymerase sigma factor
Function of RNA polymerase sigma factor
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Shine-Dalgarno sequence
Shine-Dalgarno sequence
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Spliceosome
Spliceosome
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Watson and Crick's DNA structure
Watson and Crick's DNA structure
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Amino acids in mRNA translation
Amino acids in mRNA translation
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mRNA transcript from DNA
mRNA transcript from DNA
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Alternative splicing
Alternative splicing
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5' cap function
5' cap function
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Gene vs. gene products ratio
Gene vs. gene products ratio
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Virulent bacteria transformation
Virulent bacteria transformation
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Compaction of metaphase chromosomes
Compaction of metaphase chromosomes
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Promoter region of a gene
Promoter region of a gene
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Maintaining open DNA template
Maintaining open DNA template
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Eukaryotic chromosome domains
Eukaryotic chromosome domains
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Characteristics of tRNA
Characteristics of tRNA
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Genetic code codons
Genetic code codons
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Lagging vs. Leading strand
Lagging vs. Leading strand
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Leading Strand
Leading Strand
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Lagging Strand
Lagging Strand
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Okazaki Fragments
Okazaki Fragments
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DNA Helicase
DNA Helicase
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Sigma Factor
Sigma Factor
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RNA Polymerase Features
RNA Polymerase Features
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Chargaff's Rules
Chargaff's Rules
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Helicase Speed
Helicase Speed
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Study Notes
Histones and DNA Charge
- Histones have a positive charge, which helps their interaction with DNA, which has a negative charge.
tRNA Structure and Codon Pairing
- tRNA molecules have stems and loops.
- The anticodon loop of tRNA pairs with a codon on mRNA.
- At least one, or possibly two, bases on the anticodon loop must pair exactly with the bases of the codon, due to the wobble position.
DNA Polymerase Requirements
- DNA polymerases need a primer, template DNA, and deoxynucleoside triphosphates (dNTPs) to synthesize DNA.
E. coli Genetic Material
- The genetic material in E. coli is composed of circular, double-stranded DNA.
Avery, MacLeod, and McCarty Experiment
- The DNase treatment, used by Avery, MacLeod, and McCarty, allowed them to isolate pure DNA samples.
- It demonstrated that removing DNA prevents transformation.
Adenine Content and G+C Percentage
- If DNA has a 25% adenine content, the G+C content will be 50%.
Introns
- Introns are removed from primary mRNA before translation.
- Introns form a lariat structure during splicing.
- Introns have specific nucleotide sequences at their 5' and 3' ends.
Griffith's 1928 Experiment
- Griffith's experiment demonstrated genetic transformation of non-virulent bacteria into virulent bacteria.
- Mice injected with a mixture of dead virulent bacteria and live non-virulent bacteria died.
Eukaryotic Chromosome Compaction
- Eukaryotic chromosomes involve the formation of nucleosomes, a 30nm fiber, and further compaction of radial loops.
Promoter Region
- The promoter region in a gene contains cis-acting sequences and consensus sequences for RNA polymerase binding.
DNA Replication
- Single-stranded binding proteins maintain the DNA template "open" during replication.
- DNA polymerases are also involved.
RNA
- tRNA is an RNA molecule that is encoded by tRNA genes.
- It is translated into protein in the cytoplasm.
- tRNA has secondary structure and an amino acid accepting site.
Genetic Code
- The genetic code can have multiple (more than one) codons that code for a single amino acid.
- Multiple start codons may exist.
- The genetic code is ambiguous when the same codon codes for two or more amino acids.
RNA Polymerase
- RNA polymerase needs a promoter sequence for binding to initiate transcription and synthesize mRNA.
- Sigma factor helps RNA polymerase find and bind to promoter elements.
RNA Polymerase and DNA
- RNA polymerase is responsible for transcribing DNA into RNA.
- It does not require a primer to synthesize in the 5' to 3' direction.
- RNA polymerase uses ribonucleotides as substrate.
DNA Polymerases
- DNA polymerases require a primer and template for replication to start.
Mutations and DNA Synthesis
- DNA mutations in polymerases may affect the directional synthesis of DNA (the 3' to 5' direction).
Telomerase
- Telomerase synthesizes telomeric repeats at the ends of chromosomes.
- It uses RNA as a template for DNA synthesis.
- It binds to the 3’ overhang of the telomere.
Replication Fork
- DNA replication occurs at a replication fork.
Splicing in Prokaryotes and Eukaryotes
- Splicing of transcripts occurs in the nucleus of eukaryotes but not in the cytoplasm of prokaryotes.
Telomere Mutations
- Mutations in telomerase result in a gradual decrease of chromosome length during cell division.
Transcription Factors
- General transcription factors in eukaryotes recognize the TATA box and recruit RNA polymerase II.
Spliceosome and Introns
- The spliceosome removes introns from primary mRNA.
- The spliceosome recognizes nucleotides within the introns, forming a lariat structure during splicing.
DNA Replication and RNA Enzymes
- RNA polymerases copy genetic information during DNA replication, using RNA-based primers where applicable.
Eukaryotic mRNA Structure
- Eukaryotic mRNA is capped at the 5' end to protect it and aid in transport from the nucleus.
mRNA Translation
- A critical part of mRNA involved in translation is the 5' cap.
Eukaryotic Gene Regulation
- Eukaryotic genes have a large number of introns, that interrupt the coding sequences of exons.
- This contributes to the vast number and diversity in gene products from a relatively lower number of genes.
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Description
Test your knowledge on key concepts of molecular biology, including histones, tRNA structure, DNA polymerase, and genetic material in E. coli. This quiz covers essential experiments and nucleotide composition, along with the implications of introns. Dive into the intricate world of genetics and molecular interactions!