Molecular Biology Lecture 1: Genome

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Questions and Answers

What is the reason why A-T base pairs are easier to unzip?

  • They have one hydrogen bond
  • They have four hydrogen bonds
  • They have two hydrogen bonds (correct)
  • They have three hydrogen bonds

What is the function of helicase in the replication process?

  • To synthesize RNA primers
  • To unwind and separate the DNA helix into single-stranded DNA (correct)
  • To synthesize DNA continuously
  • To join DNA fragments

How many RNA primers are needed on the leading strand?

  • Two
  • One (correct)
  • None
  • Multiple

What is the direction in which DNA polymerase synthesizes new strands?

<p>5′ to 3′ direction (C)</p> Signup and view all the answers

What is the purpose of the replication fork?

<p>To provide a Y-shaped structure for DNA replication (A)</p> Signup and view all the answers

What is the function of DNA ligase in the replication process?

<p>To join DNA fragments (D)</p> Signup and view all the answers

What is the primary function of genetic material?

<p>To encode the information for the production of proteins (C)</p> Signup and view all the answers

What are the four different monomers that make up DNA?

<p>Adenine, guanine, cytosine, and thymine (A)</p> Signup and view all the answers

What is the term for the entire genetic complement of a living organism?

<p>Genome (A)</p> Signup and view all the answers

What is the characteristic structure of DNA?

<p>Double-stranded helix (D)</p> Signup and view all the answers

What are the two types of nitrogenous bases found in DNA?

<p>Purine and pyrimidine (D)</p> Signup and view all the answers

What is the component of a nucleotide that contains a phosphorus atom bonded to four oxygen atoms?

<p>Phosphate group (C)</p> Signup and view all the answers

What is the direction of synthesis of the lagging strand relative to the replication fork?

<p>Away from the replication fork (D)</p> Signup and view all the answers

Why does the lagging strand need to be synthesized in pieces?

<p>Because the DNA polymerase can only synthesize in the 5' to 3' direction (A)</p> Signup and view all the answers

What is the role of the RNA primer in the synthesis of Okazaki fragments?

<p>To initiate the synthesis of each Okazaki fragment (C)</p> Signup and view all the answers

What determines which of the four DNA nucleotides is added to the new strand during elongation?

<p>The template strand (C)</p> Signup and view all the answers

What happens when DNA polymerase reaches a section of DNA template that has already been replicated?

<p>It halts and drops off (A)</p> Signup and view all the answers

What are the unattached sections of the sugar-phosphate backbone in an otherwise full-replicated DNA strand called?

<p>Nicks (C)</p> Signup and view all the answers

What type of bonds hold the nitrogenous bases together between DNA strands?

<p>Hydrogen bonds (C)</p> Signup and view all the answers

How many hydrogen bonds form between guanine and cytosine?

<p>Three (D)</p> Signup and view all the answers

What is the direction of the strands in a DNA double helix?

<p>One strand is 5’ to 3’ and the other is 3’ to 5’ (A)</p> Signup and view all the answers

What is the term for the process of producing two identical replicas from one original DNA molecule?

<p>DNA replication (B)</p> Signup and view all the answers

What is the name of the complex that targets the origins of DNA replication?

<p>Origin recognition complex (ORC) (B)</p> Signup and view all the answers

In which direction does DNA replication proceed from the origin of replication?

<p>In both directions (D)</p> Signup and view all the answers

Which enzymes are responsible for removing RNA primers during DNA replication?

<p>FEN1 and RNase H (A)</p> Signup and view all the answers

What is the purpose of FEN1 and RNase H in DNA replication?

<p>To remove RNA primers (B)</p> Signup and view all the answers

What fills in the gaps left by RNA primer removal?

<p>DNA polymerase (A)</p> Signup and view all the answers

What is the final stage of DNA replication?

<p>Ligation of sugar-phosphate backbones (D)</p> Signup and view all the answers

What is responsible for the high fidelity of DNA replication?

<p>Ability of DNA polymerase to efficiently select correct nucleotides (A)</p> Signup and view all the answers

What is the estimated frequency of incorrect nucleotide incorporation during DNA replication?

<p>One wrong nucleotide per 10^8-10^10 nucleotides (D)</p> Signup and view all the answers

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