Molecular Basis of Inheritance Quiz

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Questions and Answers

What are the two types of nucleic acids found in living systems?

Deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)

What is the primary function of DNA in most organisms?

DNA acts as the genetic material.

What are the three components of a nucleotide?

  • A nitrogenous base, a hexose sugar, and a phosphate group
  • A nitrogenous base, a pentose sugar, and a phosphate group (correct)
  • A nitrogenous base, a lipid, and a phosphate group
  • A nitrogenous base, a protein, and a phosphate group

What is the name of the bond that links a nitrogenous base to the OH of 1'C pentose sugar?

<p>N-glycosidic linkage</p> Signup and view all the answers

What is the name of the bond that links a phosphate group to the OH of 5'C of a nucleoside?

<p>Phosphoester linkage.</p> Signup and view all the answers

What is the term used for the end of a polynucleotide chain that has a free phosphate moiety at the 5'-end of sugar?

<p>5'-end of polynucleotide chain.</p> Signup and view all the answers

In which nucleic acid is the uracil base found?

<p>RNA</p> Signup and view all the answers

Who first identified DNA as an acidic substance present in the nucleus?

<p>Friedrich Meischer</p> Signup and view all the answers

Who proposed the Double Helix model for the structure of DNA, based on X-ray diffraction data?

<p>James Watson and Francis Crick</p> Signup and view all the answers

What is the main characteristic of the base pairing in the double-stranded DNA?

<p>They are said to be complementary to each other.</p> Signup and view all the answers

What is the term for the process of copying genetic information from one strand of the DNA into RNA?

<p>Transcription</p> Signup and view all the answers

What is the name of the enzyme that catalyzes the polymerization of deoxynucleotides during DNA replication?

<p>DNA-dependent DNA polymerase</p> Signup and view all the answers

What is the name of the region in E. coli DNA where replication originates?

<p>Origin of replication</p> Signup and view all the answers

What are the three major types of RNA found in bacteria?

<p>mRNA, tRNA, and rRNA</p> Signup and view all the answers

What are the three steps involved in the transcription process?

<p>Initiation, elongation, and termination</p> Signup and view all the answers

What is the term used for a polycistronic structural gene that is regulated by a common promoter and regulatory genes?

<p>Operon</p> Signup and view all the answers

What is the name of the enzyme that hydrolyses lactose into galactose and glucose?

<p>Beta-galactosidase</p> Signup and view all the answers

What is the name of the molecule that binds to the operator region of the lac operon and prevents transcription?

<p>Lac repressor protein</p> Signup and view all the answers

What is the name of the molecule that inactivates the lac repressor protein and allows transcription to proceed?

<p>Inducer</p> Signup and view all the answers

Which type of regulation is involved in the lac operon, where the repressor prevents transcription in the absence of lactose?

<p>Negative regulation</p> Signup and view all the answers

What is the main goal of the Human Genome Project?

<p>To sequence the entire human genome and identify all the genes in human DNA.</p> Signup and view all the answers

What is the name of the technique used to identify differences in specific regions of DNA sequences, often used in forensic science and paternity testing?

<p>DNA fingerprinting</p> Signup and view all the answers

What is the term for variations in DNA sequences that occur at a frequency greater than 0.01 in a population?

<p>DNA polymorphism</p> Signup and view all the answers

What is the name of the field of biology that deals with the rapid development of new technologies, such as computational tools for data storage, retrieval, and analysis, fueled by the Human Genome Project?

<p>Bioinformatics</p> Signup and view all the answers

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Flashcards

What is DNA?

Deoxyribonucleic acid, a long polymer of deoxyribonucleotides that carries genetic information in most organisms.

What is the length of DNA?

The number of nucleotides (or base pairs) in a DNA molecule, determining its length.

What is a nucleotide?

A nitrogenous base (Adenine, Guanine, Cytosine, or Thymine) linked to a pentose sugar (deoxyribose in DNA) and a phosphate group.

What is a dinucleotide?

Two nucleotides linked by a 3'-5' phosphodiester bond. This bond forms the backbone of DNA.

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What is chromatin?

The arrangement of DNA in eukaryotes where it's wrapped around positively charged histone proteins forming a 'beads-on-string' structure.

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What is DNA replication?

The process by which DNA makes an identical copy of itself. This ensures that each daughter cell receives a complete copy of the genome.

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What is the semiconservative model of DNA replication?

The theory stating that DNA replication produces two new DNA molecules, each containing one parental strand and one newly synthesized strand.

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What is DNA polymerase?

The enzyme responsible for catalyzing the polymerization of deoxyribonucleotides during DNA replication. It uses a DNA template to create a new strand.

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What is the origin of replication?

A specific region in DNA where replication begins. This region contains sequences that initiate the process.

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What are Okazaki fragments?

Short, discontinuous fragments of DNA synthesized on the lagging strand during DNA replication. They are later joined by DNA ligase.

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What is transcription?

The process of copying genetic information from DNA to RNA, following the principle of complementarity.

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What is a transcription unit?

A segment of DNA that contains the information for a specific RNA molecule, consisting of a promoter, structural gene, and terminator.

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What is a promoter?

A DNA sequence located upstream of the structural gene in a transcription unit. It provides a binding site for RNA polymerase, initiating transcription.

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What is a terminator?

A DNA sequence located downstream of the structural gene in a transcription unit. It signals the end of transcription.

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What is a cistron?

A segment of DNA coding for a polypeptide, the building block of a protein.

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What are exons?

Coding sequences in a gene that are present in mature RNA, determining the amino acid sequence of a protein.

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What are introns?

Non-coding sequences within a gene that are removed during RNA processing. These sequences are not translated into proteins.

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What is splicing?

The process of removing introns and joining exons in a defined order to produce a functional RNA molecule.

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What is mRNA (messenger RNA)?

A type of RNA that carries the genetic code from DNA to ribosomes, where it serves as a template for protein synthesis.

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What is tRNA (transfer RNA)?

A type of RNA molecule that brings specific amino acids to ribosomes during protein synthesis, matching the genetic code on mRNA.

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What is rRNA (ribosomal RNA)?

A type of RNA molecule that forms part of the ribosome structure, serving as a catalyst for peptide bond formation in protein synthesis.

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What is translation?

The process of forming a protein from a sequence of amino acids, guided by the genetic code on mRNA.

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What is a ribosome?

The cellular factory responsible for protein synthesis. It consists of ribosomal RNA and proteins, and has sites for mRNA and tRNA binding.

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What is gene regulation?

The control of gene expression, determining which genes are activated (on) or deactivated (off) in a cell. This regulates the production of proteins.

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What is an operon?

A cluster of genes under the control of a single promoter, commonly found in bacteria, where related genes are transcribed together.

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What is the Lac Operon?

A system of gene regulation in bacteria, where lactose acts as an inducer, switching on the expression of genes for lactose metabolism.

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What is an inducer?

A molecule that binds to a repressor protein and inactivates it, allowing gene expression. In the Lac Operon, lactose acts as an inducer.

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What is a repressor protein?

A protein that binds to the operator region of an operon, preventing RNA polymerase from transcribing the genes. In the Lac Operon, the repressor protein normally blocks lactose metabolism.

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What is an operator?

A sequence of DNA located near the promoter region of an operon, where repressor proteins can bind.

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What is the Human Genome Project?

A research project with the goal to map and sequence the entire human genome, providing valuable information about human biology and disease.

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What is DNA fingerprinting?

The process of identifying differences in DNA sequences between individuals, often using repetitive DNA regions, to create a unique pattern for identification.

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What are repetitive DNA sequences?

Regions of DNA containing repetitive sequences, often used in DNA fingerprinting to identify genetic differences.

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What is DNA polymorphism?

Variations in DNA sequences that occur commonly in a population. These differences can be used for genetic mapping and understanding disease inheritance.

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Study Notes

Molecular Basis of Inheritance

  • DNA is the genetic material for most organisms
  • DNA and RNA are nucleic acids
  • Nucleotides are the monomers of nucleic acids
  • DNA is a double helix with two antiparallel strands
  • DNA polymerase is the main enzyme in DNA replication
  • Replication is semi-conservative
  • Transcription is the process of making RNA from DNA
  • The genetic code is a triplet code that determines the amino acid sequence in proteins
  • Translation is the process of making proteins from mRNA
  • Gene expression is regulated at various levels
  • The lac operon is a model system for gene regulation in prokaryotes

DNA

  • DNA is a polymer of nucleotides
  • Each nucleotide has a phosphate group, a sugar (deoxyribose), and a nitrogenous base (A, T, G, C)
  • The double helix structure of DNA is stabilized by hydrogen bonds between complementary base pairs (A-T and G-C)
  • The two strands of the DNA run antiparallel (5' to 3' and 3' to 5')

Replication

  • DNA replication is a semi-conservative process
  • DNA polymerase is the key enzyme in this process
  • The replication fork is the region where the two strands of DNA separate
  • New DNA strands are synthesized using the parental strands as templates
  • Several proteins and enzymes are involved in DNA replication

Transcription

  • Transcription is the process of making RNA from a DNA template
  • RNA polymerase is the key enzyme
  • The process involves three stages: initiation, elongation, and termination
  • Different types of RNA are produced (mRNA, tRNA, rRNA)

Translation

  • Translation is the process of making proteins from mRNA
  • Ribosomes are the site of translation
  • Transfer RNA (tRNA) molecules bring amino acids to the ribosome
  • The genetic code is a triplet code, specifying the sequence of amino acids in a protein
  • Multiple enzymes and factors are involved in this process

Gene Regulation

  • Gene expression is the process by which information from a gene is used to synthesize a functional gene product
  • Gene regulation is the control of gene expression
  • Prokaryotes often regulate gene expression through operons (e.g., the lac operon)
  • Eukaryotes regulate gene expression at multiple levels

Human Genome Project (HGP)

  • The HGP was an international project to determine the complete DNA sequence of the human genome
  • A major goal was to identify all human genes and determine their sequences
  • The project revealed that the human genome has a relatively small number of genes (compared to previous estimates)
  • Many genes are represented by multiple copies of DNA segments
  • Many of the discovered genes were not known to function.

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