MLS 404 Laboratory Materials & Instrumentation Quiz: Centrifuge

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Questions and Answers

What is the purpose of calibrating the centrifuge using a tachometer every 3 months?

To adjust the angle of the tubes during centrifugation
To disinfect the centrifuge and prevent contamination
To ensure the speed of the centrifuge is accurate and consistent (correct)
To generate high speeds for tight cell packing

Which type of centrifuge is recommended for serum separator tubes?

Swinging bucket type
Horizontal Centrifuge (correct)
Angle Head Centrifuge
Ultracentrifuge

What is the purpose of an ultracentrifuge being refrigerated?

To disinfect the centrifuge and prevent contamination
To generate the highest speeds
To adjust the angle of the tubes during centrifugation
To reduce heat build up produced by friction (correct)

What is RCF (Relative Centrifugal Force) in a centrifuge?

Force needed to separate the components of a sample or solution (B) Signup and view all the answers

What type of centrifuge generates less heat due to air friction during the centrifugation process?

Angle Head Centrifuge (C) Signup and view all the answers

What type of glassware has the highest thermal resistance?

Boron-free glassware (A) Signup and view all the answers

Which type of pipet allows the exact volume to be obtained by blowing out the last drop?

Serologic pipet (D) Signup and view all the answers

What is the primary structure of a protein?

Linear sequence of amino acids (C) Signup and view all the answers

Which type of glassware is suitable for drastic heat shock and extreme chemical treatment?

Flint glassware (A) Signup and view all the answers

What does a self-draining pipet allow the liquid to do?

Drain by gravity (D) Signup and view all the answers

What is the purpose of calibrating the centrifuge using a tachometer every 3 months?

To ensure that the centrifuge reaches the recommended RPM for efficient separation (B) Signup and view all the answers

What is RCF (Relative Centrifugal Force) in a centrifuge?

The force needed to separate the components of a sample or solution (B) Signup and view all the answers

Which type of centrifuge is recommended for lipoprotein separation?

Ultracentrifuge (A) Signup and view all the answers

What type of glassware has the highest thermal resistance?

Borosilicate glassware (B) Signup and view all the answers

Which type of centrifuge generates less heat due to air friction during the centrifugation process?

Angle Head Centrifuge (B) Signup and view all the answers

What is the purpose of a self-draining pipet?

To allow the liquid to drain by gravity without etched rings (B) Signup and view all the answers

Which type of glassware has less thermal resistance than borosilicate glass?

Flint glass (C) Signup and view all the answers

What is the tertiary structure of a protein responsible for?

Folding pattern of the protein affecting physical and chemical properties (B) Signup and view all the answers

What does a blown out pipet with continuous etched rings allow?

Obtaining an exact volume by blowing out the last drop (D) Signup and view all the answers

What is the primary function of alumina-silicate glass (Vycor) in comparison to borosilicate glass?

It is 6 times stronger than borosilicate glass and chemically strengthened (D) Signup and view all the answers

Flashcards

Centrifuge Calibration

The process of verifying the centrifuge's speed accuracy using a tachometer every three months.

Horizontal Centrifuge

A type of centrifuge that spins tubes horizontally, ideal for serum separation tubes.

Ultracentrifuge

A centrifuge that uses high speeds to separate components based on density, typically used for lipoprotein separation.