MLS 404 Laboratory Materials & Instrumentation Quiz: Centrifuge
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Questions and Answers

What is the purpose of calibrating the centrifuge using a tachometer every 3 months?

  • To adjust the angle of the tubes during centrifugation
  • To disinfect the centrifuge and prevent contamination
  • To ensure the speed of the centrifuge is accurate and consistent (correct)
  • To generate high speeds for tight cell packing
  • Which type of centrifuge is recommended for serum separator tubes?

  • Swinging bucket type
  • Horizontal Centrifuge (correct)
  • Angle Head Centrifuge
  • Ultracentrifuge
  • What is the purpose of an ultracentrifuge being refrigerated?

  • To disinfect the centrifuge and prevent contamination
  • To generate the highest speeds
  • To adjust the angle of the tubes during centrifugation
  • To reduce heat build up produced by friction (correct)
  • What is RCF (Relative Centrifugal Force) in a centrifuge?

    <p>Force needed to separate the components of a sample or solution (B)</p> Signup and view all the answers

    What type of centrifuge generates less heat due to air friction during the centrifugation process?

    <p>Angle Head Centrifuge (C)</p> Signup and view all the answers

    What type of glassware has the highest thermal resistance?

    <p>Boron-free glassware (A)</p> Signup and view all the answers

    Which type of pipet allows the exact volume to be obtained by blowing out the last drop?

    <p>Serologic pipet (D)</p> Signup and view all the answers

    What is the primary structure of a protein?

    <p>Linear sequence of amino acids (C)</p> Signup and view all the answers

    Which type of glassware is suitable for drastic heat shock and extreme chemical treatment?

    <p>Flint glassware (A)</p> Signup and view all the answers

    What does a self-draining pipet allow the liquid to do?

    <p>Drain by gravity (D)</p> Signup and view all the answers

    What is the purpose of calibrating the centrifuge using a tachometer every 3 months?

    <p>To ensure that the centrifuge reaches the recommended RPM for efficient separation (B)</p> Signup and view all the answers

    What is RCF (Relative Centrifugal Force) in a centrifuge?

    <p>The force needed to separate the components of a sample or solution (B)</p> Signup and view all the answers

    Which type of centrifuge is recommended for lipoprotein separation?

    <p>Ultracentrifuge (A)</p> Signup and view all the answers

    What type of glassware has the highest thermal resistance?

    <p>Borosilicate glassware (B)</p> Signup and view all the answers

    Which type of centrifuge generates less heat due to air friction during the centrifugation process?

    <p>Angle Head Centrifuge (B)</p> Signup and view all the answers

    What is the purpose of a self-draining pipet?

    <p>To allow the liquid to drain by gravity without etched rings (B)</p> Signup and view all the answers

    Which type of glassware has less thermal resistance than borosilicate glass?

    <p>Flint glass (C)</p> Signup and view all the answers

    What is the tertiary structure of a protein responsible for?

    <p>Folding pattern of the protein affecting physical and chemical properties (B)</p> Signup and view all the answers

    What does a blown out pipet with continuous etched rings allow?

    <p>Obtaining an exact volume by blowing out the last drop (D)</p> Signup and view all the answers

    What is the primary function of alumina-silicate glass (Vycor) in comparison to borosilicate glass?

    <p>It is 6 times stronger than borosilicate glass and chemically strengthened (D)</p> Signup and view all the answers

    Flashcards

    Centrifuge Calibration

    The process of verifying the centrifuge's speed accuracy using a tachometer every three months.

    Horizontal Centrifuge

    A type of centrifuge that spins tubes horizontally, ideal for serum separation tubes.

    Ultracentrifuge

    A centrifuge that uses high speeds to separate components based on density, typically used for lipoprotein separation.

    Relative Centrifugal Force (RCF)

    The force applied to separate components in a centrifuge, measured as g-force.

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    Angle Head Centrifuge

    A type of centrifuge where tubes are angled, reducing heat generation due to air friction.

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    Borosilicate Glassware

    A type of glassware known for its high resistance to heat, chemicals, and thermal shock.

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    Serologic Pipet

    A type of pipet with continuous etched rings that allows for precise volume measurement by blowing out the last drop.

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    Primary Structure of a protein

    The linear sequence of amino acids in a protein, forming its basic structure.

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    Flint Glassware

    A type of glassware with lower thermal resistance compared to borosilicate glass.

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    Self-Draining Pipet

    A feature in some pipets that allows the liquid to drain by gravity without the need for blowing out.

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    Blown Out Pipet with Etched Rings

    A type of pipet with etched rings that allows for precise volume measurement by blowing out the last drop.

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    Alumina-Silicate Glass (Vycor)

    A type of glass known for its high strength and chemical resistance, six times stronger than borosilicate glass.

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    Tertiary Structure of a Protein

    The three-dimensional folding pattern of a protein that impacts its physical and chemical properties.

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    Ultracentrifuge

    A centrifuge designed for ultra-high speeds, used to separate particles based on their density.

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    Relative Centrifugal Force (RCF)

    The force exerted on particles in a centrifuge, measured as g-force, used to determine the separation efficiency.

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    Angle Head Centrifuge

    A type of centrifuge where tubes are positioned at an angle, reducing heat generation.

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    Borosilicate Glassware

    A type of glassware renowned for its high resistance to heat, chemicals, and thermal shock.

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    Serologic Pipet

    A type of pipet with continuous etched markings indicating the precise volume, requiring blowing out the last drop.

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    Flint Glassware

    A type of glassware with lower heat resistance compared to borosilicate glass.

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    Self-Draining Pipet

    A feature in pipets that allows for automatic drainage without blowing, ideal for precise fluid dispensing.

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