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MLS 221/L-CC1: Glucose-6-Phosphate Reaction

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MLS 221/L-CC1: Glucose-6-Phosphate Reaction

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Questions and Answers

What is the product of the reaction catalyzed by glucose oxidase?

Glucose-6-phosphate and NADPH

Gluconic acid and hydrogen peroxide (correct)

6-Phosphogluconate and NADP

Gluconic acid and NADPH

What is the purpose of adding peroxidase and a chromogenic oxygen acceptor in the glucose oxidase-peroxidase method?

To reduce NADP to NADPH

To convert gluconic acid to glucose

To oxidize glucose to gluconic acid

To form a colored compound proportional to the amount of glucose present (correct)

What is the significance of the formation of NADPH in the glucose-6-phosphate reaction?

It is proportional to the amount of glucose present (correct)

It indicates the presence of gluconic acid

It is the substrate for the glucose oxidase reaction

It reacts with peroxidase to form a colored compound

Why are anticoagulants such as fluoride, heparin, oxalate, and EDTA commonly used in glucose analysis?

To inhibit glucose metabolism in the sample Signup and view all the answers

What is the advantage of the glucose-6-phosphate method compared to the glucose oxidase-peroxidase method?

It is less subject to interference Signup and view all the answers

What is the specific substrate for the enzyme Glucose Dehydrogenase?

β-D-glucose Signup and view all the answers

Why is mutarotase required in the glucose dehydrogenase reaction?

To convert α-D-glucose to β-D-glucose Signup and view all the answers

In which specimen type is the glucose dehydrogenase reaction not suitable?

Urine Signup and view all the answers

What is the purpose of the oral glucose tolerance test (OGTT)?

To diagnose diabetes mellitus in patients with symptoms of hyperglycemia Signup and view all the answers

What is the cut-off value for fasting blood glucose to diagnose diabetes mellitus?

126 mg/dL Signup and view all the answers

What is the preparation required for the oral glucose tolerance test (OGTT)?

Fast of at least 8 hours Signup and view all the answers

What percentage of plasma glucose concentration is approximately found in CSF glucose?

60% Signup and view all the answers

Why should CSF glucose be analyzed immediately?

To prevent contamination with bacteria Signup and view all the answers

What is the recommended storage temperature for urine during collection to prevent glucose loss?

4 °C Signup and view all the answers

What is the principle behind the quantitative measurement of glucose in blood?

Hexokinase ATP is simultaneously converted to adenosine diphosphate (ADP) Signup and view all the answers

What is the purpose of citrate buffer in collection tubes?

To inhibit glycolysis Signup and view all the answers

What is the relationship between peritoneal fluid glucose and plasma glucose levels?

Peritoneal fluid glucose is equal to plasma glucose Signup and view all the answers