Podcast
Questions and Answers
What is the product of the reaction catalyzed by glucose oxidase?
What is the product of the reaction catalyzed by glucose oxidase?
- Glucose-6-phosphate and NADPH
- Gluconic acid and hydrogen peroxide (correct)
- 6-Phosphogluconate and NADP
- Gluconic acid and NADPH
What is the purpose of adding peroxidase and a chromogenic oxygen acceptor in the glucose oxidase-peroxidase method?
What is the purpose of adding peroxidase and a chromogenic oxygen acceptor in the glucose oxidase-peroxidase method?
- To reduce NADP to NADPH
- To convert gluconic acid to glucose
- To oxidize glucose to gluconic acid
- To form a colored compound proportional to the amount of glucose present (correct)
What is the significance of the formation of NADPH in the glucose-6-phosphate reaction?
What is the significance of the formation of NADPH in the glucose-6-phosphate reaction?
- It is proportional to the amount of glucose present (correct)
- It indicates the presence of gluconic acid
- It is the substrate for the glucose oxidase reaction
- It reacts with peroxidase to form a colored compound
Why are anticoagulants such as fluoride, heparin, oxalate, and EDTA commonly used in glucose analysis?
Why are anticoagulants such as fluoride, heparin, oxalate, and EDTA commonly used in glucose analysis?
What is the advantage of the glucose-6-phosphate method compared to the glucose oxidase-peroxidase method?
What is the advantage of the glucose-6-phosphate method compared to the glucose oxidase-peroxidase method?
What is the specific substrate for the enzyme Glucose Dehydrogenase?
What is the specific substrate for the enzyme Glucose Dehydrogenase?
Why is mutarotase required in the glucose dehydrogenase reaction?
Why is mutarotase required in the glucose dehydrogenase reaction?
In which specimen type is the glucose dehydrogenase reaction not suitable?
In which specimen type is the glucose dehydrogenase reaction not suitable?
What is the purpose of the oral glucose tolerance test (OGTT)?
What is the purpose of the oral glucose tolerance test (OGTT)?
What is the cut-off value for fasting blood glucose to diagnose diabetes mellitus?
What is the cut-off value for fasting blood glucose to diagnose diabetes mellitus?
What is the preparation required for the oral glucose tolerance test (OGTT)?
What is the preparation required for the oral glucose tolerance test (OGTT)?
What percentage of plasma glucose concentration is approximately found in CSF glucose?
What percentage of plasma glucose concentration is approximately found in CSF glucose?
Why should CSF glucose be analyzed immediately?
Why should CSF glucose be analyzed immediately?
What is the recommended storage temperature for urine during collection to prevent glucose loss?
What is the recommended storage temperature for urine during collection to prevent glucose loss?
What is the principle behind the quantitative measurement of glucose in blood?
What is the principle behind the quantitative measurement of glucose in blood?
What is the purpose of citrate buffer in collection tubes?
What is the purpose of citrate buffer in collection tubes?
What is the relationship between peritoneal fluid glucose and plasma glucose levels?
What is the relationship between peritoneal fluid glucose and plasma glucose levels?
